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The map label for this gene is spsK [H]

Identifier: 116625364

GI number: 116625364

Start: 7926272

End: 7927156

Strand: Reverse

Name: spsK [H]

Synonym: Acid_6309

Alternate gene names: 116625364

Gene position: 7927156-7926272 (Counterclockwise)

Preceding gene: 116625365

Following gene: 116625362

Centisome position: 79.54

GC content: 64.52

Gene sequence:

>885_bases
ATGAGTAGACGCGCTGTAGTATTCGGAGCTGCCGGCCAGCTGGGGATCGAGCTGGTCCGCGAACTTCAGCAGCGGCGGTA
CACCGTGATCTCATGGGATCGCGCCCAGGTAGACATCACGGATCCGGTGGCGGTGGAAAATGCGCTGGCCAACTACGACG
CCGAGGTAGTCTTCAATGCAGCCGCCTACAATCAGGTCGATGTAGCGGAGAAGGAGCCCCAGGCGGCGTTCCTGGTGAAC
GCTCTGGCGGTTCGCAACCTGGCCCTCGCATGCCGTCAGGCGGACGCGCAACTGGTACATTTCTCAACGGATTACGTTTT
CGATGGGGCCGCTCGGCATCCCTACGTGGAGGAGGACCCCACGCACCCGCTGGGGGCGTATGCGGTCTCCAAGCTTGCGG
GCGAACTCTATGCGCAGGCCTACCTGGACCGGGCCCTGGTGGTGCGGACCTCCGGAGTTTTCGGTCCCGGGGGGTTAACC
ACGGCCCGCGGCAATTTCGTGGAGCTGATGCTGCGCCTGGCGGCTTCGCCCAATCCGATCCGGGTGGTCGAGGACCACGT
CGCCAGCCCGACTTTCGCGCCGCTGCTGGCATCGCGCACCATCGATCTGGTGGAGCGCGGGATTGCAGGACTGTTTCATA
TCGGCGGCGGCGCCCCGGTATCCTGGTTCCAGTTTGCCCGGCTGATCTTCGAGGCTGCGGGATTGAAGCCCACGCTGCTG
GCTACCAACGAGCGCGAATACCGGACTCCCGCGCGCCGGCCGAAGTACAGTGCGCTTTCCAATGCGAAGCTGGAGCGCAA
TGGAGTCGCGGCCATGCCGGCACTGAAGGACGTGTTGGTTACCTACTTCGAAGCGCGGGGACAGGCCGTGACCCGGGAGC
GGTGA

Upstream 100 bases:

>100_bases
TTGCTCCCTATTCTGACTAGAATAACGCATTAGAAATGCTTCCGGTTACATACAAGGGTGCTGCCGCACGCGTTCAGGTT
GCGCTATCGTGGTGTTTCAT

Downstream 100 bases:

>100_bases
GTTTCACGCGAGCTTCCTTTTCTCGCAGAGCCGCAGAGCCGCAGAGAAACAAGGCAAGAATTCCTTCAAGGTTTTTCTTC
TTGGCGTTACTCTGCGGCTC

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSRRAVVFGAAGQLGIELVRELQQRRYTVISWDRAQVDITDPVAVENALANYDAEVVFNAAAYNQVDVAEKEPQAAFLVN
ALAVRNLALACRQADAQLVHFSTDYVFDGAARHPYVEEDPTHPLGAYAVSKLAGELYAQAYLDRALVVRTSGVFGPGGLT
TARGNFVELMLRLAASPNPIRVVEDHVASPTFAPLLASRTIDLVERGIAGLFHIGGGAPVSWFQFARLIFEAAGLKPTLL
ATNEREYRTPARRPKYSALSNAKLERNGVAAMPALKDVLVTYFEARGQAVTRER

Sequences:

>Translated_294_residues
MSRRAVVFGAAGQLGIELVRELQQRRYTVISWDRAQVDITDPVAVENALANYDAEVVFNAAAYNQVDVAEKEPQAAFLVN
ALAVRNLALACRQADAQLVHFSTDYVFDGAARHPYVEEDPTHPLGAYAVSKLAGELYAQAYLDRALVVRTSGVFGPGGLT
TARGNFVELMLRLAASPNPIRVVEDHVASPTFAPLLASRTIDLVERGIAGLFHIGGGAPVSWFQFARLIFEAAGLKPTLL
ATNEREYRTPARRPKYSALSNAKLERNGVAAMPALKDVLVTYFEARGQAVTRER
>Mature_293_residues
SRRAVVFGAAGQLGIELVRELQQRRYTVISWDRAQVDITDPVAVENALANYDAEVVFNAAAYNQVDVAEKEPQAAFLVNA
LAVRNLALACRQADAQLVHFSTDYVFDGAARHPYVEEDPTHPLGAYAVSKLAGELYAQAYLDRALVVRTSGVFGPGGLTT
ARGNFVELMLRLAASPNPIRVVEDHVASPTFAPLLASRTIDLVERGIAGLFHIGGGAPVSWFQFARLIFEAAGLKPTLLA
TNEREYRTPARRPKYSALSNAKLERNGVAAMPALKDVLVTYFEARGQAVTRER

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=286, Percent_Identity=30.4195804195804, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI33519455, Length=286, Percent_Identity=30.4195804195804, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1788352, Length=283, Percent_Identity=36.0424028268551, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32040; Mature: 31909

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRAVVFGAAGQLGIELVRELQQRRYTVISWDRAQVDITDPVAVENALANYDAEVVFNA
CCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCEEECCCCHHHHHHHHCCCCEEEEEE
AAYNQVDVAEKEPQAAFLVNALAVRNLALACRQADAQLVHFSTDYVFDGAARHPYVEEDP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCCCCCCCCCCC
THPLGAYAVSKLAGELYAQAYLDRALVVRTSGVFGPGGLTTARGNFVELMLRLAASPNPI
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCE
RVVEDHVASPTFAPLLASRTIDLVERGIAGLFHIGGGAPVSWFQFARLIFEAAGLKPTLL
EHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCCCEEE
ATNEREYRTPARRPKYSALSNAKLERNGVAAMPALKDVLVTYFEARGQAVTRER
ECCCHHHCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SRRAVVFGAAGQLGIELVRELQQRRYTVISWDRAQVDITDPVAVENALANYDAEVVFNA
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCEEECCCCHHHHHHHHCCCCEEEEEE
AAYNQVDVAEKEPQAAFLVNALAVRNLALACRQADAQLVHFSTDYVFDGAARHPYVEEDP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCCCCCCCCCCC
THPLGAYAVSKLAGELYAQAYLDRALVVRTSGVFGPGGLTTARGNFVELMLRLAASPNPI
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCE
RVVEDHVASPTFAPLLASRTIDLVERGIAGLFHIGGGAPVSWFQFARLIFEAAGLKPTLL
EHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCCCEEE
ATNEREYRTPARRPKYSALSNAKLERNGVAAMPALKDVLVTYFEARGQAVTRER
ECCCHHHCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]