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The map label for this gene is pyrG [H]

Identifier: 116625270

GI number: 116625270

Start: 7792676

End: 7794316

Strand: Reverse

Name: pyrG [H]

Synonym: Acid_6215

Alternate gene names: 116625270

Gene position: 7794316-7792676 (Counterclockwise)

Preceding gene: 116625271

Following gene: 116625269

Centisome position: 78.21

GC content: 61.0

Gene sequence:

>1641_bases
ATGGCTAAATACATATTCGTAACCGGCGGCGTGGTCTCCTCTTTGGGGAAAGGGATTGCGGCCGCGTCGATCGGTTGCCT
GCTGGAGAGCCGCGGACTCAAAGTCACGTGCCAGAAGTTCGATCCGTACCTGAATGTAGACCCGGGCACAATGAGCCCGT
TCCAGCACGGCGAAGTCTTTGTCACCGACGATGGGGCGGAGACCGATCTCGACCTGGGCCACTACGAGCGCTTCACGCAC
TCCAAGCTGACGCAGAGCAACAACCTGACCAGCGGCCGAATCTACGAGCAGATCATCGCGCGCGAACGCCGCGGCGATTA
TCTCGGCAAGACGGTGCAAGTAATTCCGCACGTCACCAATGAAATCAAAGCGGCGATGAAAAAAGTGTCCCAGGATGTGG
ACGTGGTGATCGCCGAAATCGGCGGCACCGTGGGCGACATCGAATCCCTGCCGTTCCTCGAGGCCATCCGCCAGATGCGC
CACGAGCAGGGCCGCGACAACTGTCTGTTCGTGCACGTCACTCTGGTGCCGTGGATCGCCGCCGCACAGGAATTGAAGAC
CAAGCCGACGCAGCACAGCGTGAAGGAGCTGCGCGCGCTCGGTATCCAACCGGACATTCTGCTTTGCCGCTGCGAGCGCT
TTATCCCCGAGGACATGAAGGAGAAGATCGCGCTCTTCTGCGACGTGGACGTACAGGCCGTGATCACGGCGCGCGACGTG
AAGAGCGTGTACGAAGTTCCGGTGGGCTTCGCCGCCGAGGGCGTGGACGAGATCGCCATGCGGCTGCTCAAACTGGAAGC
CGGGCCGCGGGACCTCGCCAAATGGAATGCGATGCTGGATCGCATCGAACAGCCGAAGGGCGAAGTTTCCATCGCGCTGG
TCGGCAAATACGTGGAGTATGAGGACTCCTACAAGAGCCTCAAGGAAGCACTGCTGCACGGCGGGCTGGCGCATCAATTG
AAGGTCAACATTCACTGGATCGAGGCCGAGGGCGTCGCCGGTGAAAATTGGGAGCGGCAGCTGGAGGACTACGATGGCGT
GCTGGTGCCGGGCGGTTTCGGCAAGCGCGGCATCGACGGCATGCTCAACGCGATCCGCTTCGCGCGCGAGAACAAGGTGC
CGTACTTCGGCATCTGCCTCGGTATGCAGACGCTCGTGATCGAGTTCGCACGCAACGTCTGCGGTCTCGAAGCAGCCGAT
TCCACCGAGTTCAACAGCGGCACCCCGCATCGTGTGATTTTCAAGCTGCGTGAGCTGAAGGGCATCGACGAGTTGGGCGG
CACCATGCGGCTGGGCAGTTGGGCCTGCAAACTGGCCGAAGGCAGCTTCGCGCACAAGGCCTACGGCACGCGCGAGATTA
ACGAACGCCATCGCCACCGCTATGAGTTCAATCGCGAATATGAAGACCGCCTGAAAGCCGGCGGCCTGAAGATCACCGGC
GAAACGCCCGACAACACCTACGTCGAGATCTGCGAGCTCGGCGACCACCCCTGGTTCCTGGGCTGCCAGTTCCATCCCGA
ATTCAAGAGCAAGCCGATGGAACCGCATCCGTTGTTCAAGGCCTTCATCGGCGCCGCATGGGAATATCGCATGCGAAGGC
TATCGGCGATTGAGGAGCCCCTGTTCTCCCGTGCCGATTGA

Upstream 100 bases:

>100_bases
GTGGTGGAAACCGAATACGAGTCGTTGGGCGTGGATACGCCGGAGGATCTGGAAAGGGTGAGCAGATTGTTCAAAGCCTC
AATCTTACAAGGGATGGGAA

Downstream 100 bases:

>100_bases
GTTCAGCGATTTCGGCAAGCGGCTGGTCCTGATCGCGGGACCGTGCGTGATCGAGAGCGAAGAGCACGTCCACCGCATGG
CGCGCGGCATCCGCGCCGCG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MAKYIFVTGGVVSSLGKGIAAASIGCLLESRGLKVTCQKFDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTH
SKLTQSNNLTSGRIYEQIIARERRGDYLGKTVQVIPHVTNEIKAAMKKVSQDVDVVIAEIGGTVGDIESLPFLEAIRQMR
HEQGRDNCLFVHVTLVPWIAAAQELKTKPTQHSVKELRALGIQPDILLCRCERFIPEDMKEKIALFCDVDVQAVITARDV
KSVYEVPVGFAAEGVDEIAMRLLKLEAGPRDLAKWNAMLDRIEQPKGEVSIALVGKYVEYEDSYKSLKEALLHGGLAHQL
KVNIHWIEAEGVAGENWERQLEDYDGVLVPGGFGKRGIDGMLNAIRFARENKVPYFGICLGMQTLVIEFARNVCGLEAAD
STEFNSGTPHRVIFKLRELKGIDELGGTMRLGSWACKLAEGSFAHKAYGTREINERHRHRYEFNREYEDRLKAGGLKITG
ETPDNTYVEICELGDHPWFLGCQFHPEFKSKPMEPHPLFKAFIGAAWEYRMRRLSAIEEPLFSRAD

Sequences:

>Translated_546_residues
MAKYIFVTGGVVSSLGKGIAAASIGCLLESRGLKVTCQKFDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTH
SKLTQSNNLTSGRIYEQIIARERRGDYLGKTVQVIPHVTNEIKAAMKKVSQDVDVVIAEIGGTVGDIESLPFLEAIRQMR
HEQGRDNCLFVHVTLVPWIAAAQELKTKPTQHSVKELRALGIQPDILLCRCERFIPEDMKEKIALFCDVDVQAVITARDV
KSVYEVPVGFAAEGVDEIAMRLLKLEAGPRDLAKWNAMLDRIEQPKGEVSIALVGKYVEYEDSYKSLKEALLHGGLAHQL
KVNIHWIEAEGVAGENWERQLEDYDGVLVPGGFGKRGIDGMLNAIRFARENKVPYFGICLGMQTLVIEFARNVCGLEAAD
STEFNSGTPHRVIFKLRELKGIDELGGTMRLGSWACKLAEGSFAHKAYGTREINERHRHRYEFNREYEDRLKAGGLKITG
ETPDNTYVEICELGDHPWFLGCQFHPEFKSKPMEPHPLFKAFIGAAWEYRMRRLSAIEEPLFSRAD
>Mature_545_residues
AKYIFVTGGVVSSLGKGIAAASIGCLLESRGLKVTCQKFDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTHS
KLTQSNNLTSGRIYEQIIARERRGDYLGKTVQVIPHVTNEIKAAMKKVSQDVDVVIAEIGGTVGDIESLPFLEAIRQMRH
EQGRDNCLFVHVTLVPWIAAAQELKTKPTQHSVKELRALGIQPDILLCRCERFIPEDMKEKIALFCDVDVQAVITARDVK
SVYEVPVGFAAEGVDEIAMRLLKLEAGPRDLAKWNAMLDRIEQPKGEVSIALVGKYVEYEDSYKSLKEALLHGGLAHQLK
VNIHWIEAEGVAGENWERQLEDYDGVLVPGGFGKRGIDGMLNAIRFARENKVPYFGICLGMQTLVIEFARNVCGLEAADS
TEFNSGTPHRVIFKLRELKGIDELGGTMRLGSWACKLAEGSFAHKAYGTREINERHRHRYEFNREYEDRLKAGGLKITGE
TPDNTYVEICELGDHPWFLGCQFHPEFKSKPMEPHPLFKAFIGAAWEYRMRRLSAIEEPLFSRAD

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=548, Percent_Identity=47.0802919708029, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI28559085, Length=548, Percent_Identity=45.985401459854, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI28559083, Length=548, Percent_Identity=45.985401459854, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI221316689, Length=548, Percent_Identity=45.985401459854, Blast_Score=473, Evalue=1e-133,
Organism=Escherichia coli, GI1789142, Length=542, Percent_Identity=53.1365313653137, Blast_Score=581, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI25148299, Length=603, Percent_Identity=38.971807628524, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6322563, Length=560, Percent_Identity=43.75, Blast_Score=461, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6319432, Length=561, Percent_Identity=42.9590017825312, Blast_Score=453, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24664469, Length=548, Percent_Identity=45.4379562043796, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI21357815, Length=494, Percent_Identity=43.5222672064777, Blast_Score=395, Evalue=1e-110,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 61217; Mature: 61086

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYIFVTGGVVSSLGKGIAAASIGCLLESRGLKVTCQKFDPYLNVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTHSKLTQSNNLTSGRIYEQIIARERRGDYLGKTVQVIPHVTN
EECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHCCHHHHHHHHHH
EIKAAMKKVSQDVDVVIAEIGGTVGDIESLPFLEAIRQMRHEQGRDNCLFVHVTLVPWIA
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
AAQELKTKPTQHSVKELRALGIQPDILLCRCERFIPEDMKEKIALFCDVDVQAVITARDV
HHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHEEEEECCCCEEEEEHHHH
KSVYEVPVGFAAEGVDEIAMRLLKLEAGPRDLAKWNAMLDRIEQPKGEVSIALVGKYVEY
HHHHHCCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHH
EDSYKSLKEALLHGGLAHQLKVNIHWIEAEGVAGENWERQLEDYDGVLVPGGFGKRGIDG
HHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCCHHHHHHCCCCEEECCCCCCCCHHH
MLNAIRFARENKVPYFGICLGMQTLVIEFARNVCGLEAADSTEFNSGTPHRVIFKLRELK
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHC
GIDELGGTMRLGSWACKLAEGSFAHKAYGTREINERHRHRYEFNREYEDRLKAGGLKITG
CHHHHCCCEEECHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCEEEEC
ETPDNTYVEICELGDHPWFLGCQFHPEFKSKPMEPHPLFKAFIGAAWEYRMRRLSAIEEP
CCCCCCEEEEEECCCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LFSRAD
HHCCCC
>Mature Secondary Structure 
AKYIFVTGGVVSSLGKGIAAASIGCLLESRGLKVTCQKFDPYLNVDPGTMSPFQHGEVF
CEEEEEECHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTHSKLTQSNNLTSGRIYEQIIARERRGDYLGKTVQVIPHVTN
EECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHCCHHHHHHHHHH
EIKAAMKKVSQDVDVVIAEIGGTVGDIESLPFLEAIRQMRHEQGRDNCLFVHVTLVPWIA
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
AAQELKTKPTQHSVKELRALGIQPDILLCRCERFIPEDMKEKIALFCDVDVQAVITARDV
HHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHEEEEECCCCEEEEEHHHH
KSVYEVPVGFAAEGVDEIAMRLLKLEAGPRDLAKWNAMLDRIEQPKGEVSIALVGKYVEY
HHHHHCCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHH
EDSYKSLKEALLHGGLAHQLKVNIHWIEAEGVAGENWERQLEDYDGVLVPGGFGKRGIDG
HHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCCHHHHHHCCCCEEECCCCCCCCHHH
MLNAIRFARENKVPYFGICLGMQTLVIEFARNVCGLEAADSTEFNSGTPHRVIFKLRELK
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHC
GIDELGGTMRLGSWACKLAEGSFAHKAYGTREINERHRHRYEFNREYEDRLKAGGLKITG
CHHHHCCCEEECHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCEEEEC
ETPDNTYVEICELGDHPWFLGCQFHPEFKSKPMEPHPLFKAFIGAAWEYRMRRLSAIEEP
CCCCCCEEEEEECCCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LFSRAD
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA