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The map label for this gene is aprF [H]

Identifier: 116625057

GI number: 116625057

Start: 7511411

End: 7512745

Strand: Direct

Name: aprF [H]

Synonym: Acid_5987

Alternate gene names: 116625057

Gene position: 7511411-7512745 (Clockwise)

Preceding gene: 116625056

Following gene: 116625058

Centisome position: 75.37

GC content: 66.29

Gene sequence:

>1335_bases
ATGGGACGGGTTTGGATTCTATTGGCGGCCGCGGCACTGCCTGCGACACTGCCCGCCATTCTGGCGGCGGCTCCGGTGGA
GCTGAGTTTGAAGCGGGCGGTGCAACTGGCAGTTTCGCCGGAGGGCAACACGCGCGTGCAACTCTCGGGCGAGGCGTTGA
AGCAGGCGCAATCGAGGTCGCTCGAATCGCGCGCGGCACTGCTGCCCGATTTGTCGGCTGCCTACACGCAGCAGAACATG
ACGCGCAACCTGGGAGCGATGGGAATCCATTTGACATCGCCGTTCCCGGGATTTCAGATTCCGCAATTCGTAGGGCCGTT
CACGACGCTGGACGGGCGGGTTTCGGCGACGCAAAGCGTGTTCGATATCAGCGCGTACCGGCGCTACCAGGCATCGAAGG
CGGCGGTGGGCGCGGCGCAGGGCGATTTGAAGTCTTCGGGAGAGCAGGTGGCGGCGCTGGTGGCGCGAGCCTATCTGGCT
GCGTTGCGCGCGGATGCGGACGTGGAGACGGCCAGGGCTAATGTAGCGCTCTCAGAGGCGGTGCGGAAGCAGGCGGAAGA
CCAGAAAGCGGCGGGCACCGGCACCGGAATTGAGATCACGCGGACGCGGGTGCAGTTGGCCAACGACCGGCAGCGTTTGC
TGGTGGCGGAAAATGCGCGGCGCAGCGCGGCGCTGCAGTTGTTGCGCGCTATCGGGATGCGGCTGGATACGGAACTGGTG
CTGACGGATAAGCTCGAGGCCACGCCGGTGGATGCGATGACGATTCAGCAGGCCAAGGCGATGGCGCTCGAAAGCCGGCC
AGACTACCAGGCGCAGCGCGAGCGCGAGGCCACGGCGCGGATTTCGGCGGATGCCGTGAAGCTGGAGCGCCTGCCCACCG
TAGCGGCATTCGGAGATTACGGGGAGAGCGGCACGGGATTCAACAGCTCGCTGCCCACGCGCACGATCGGGATCACAGTA
CGGATCCCGGTGTTCGACGGCGGGAGGCGCGAGGCGTGGCGAGCGGAATCGGCATCGCAGGCGCGGTCGGAGAAGGTGAA
GACCAGCGACCTGAAAGAGCAGATCGAGTTGGACGTTCGCCTGGCGCTGGATGCGCTCCGGTCGGCGGAGGAACAGGTGA
AGGTCTCGAGGGAAGGGCTGGAACTGGCCGAGAACGAGGTTACGCAAGCGCGCAGGCGCTACGACGCCGGCGTAGCGGCG
GGCCTCGAGGTGACCGACGCGCAGACGCGGCTGGAGCGCGCGCGGGACAACCAGACGGCGGCGCTCTACAACTACAACGT
GGCGAGAATCGACCTGGCGCAATCCGTAGGCGCGGTACGCAGGATGGTGGAGTGA

Upstream 100 bases:

>100_bases
GGCGATGGAACCGAACGCGGACTATGCCCTGCGGATGAAGCGGCTGGTAGCGTTCTTGAGCGCGGGTTTTCGTGCTCCGG
CGGCAAGCGAGGAGAAGTAG

Downstream 100 bases:

>100_bases
CGAAATGAAGAAACGAATTCCGATTCTATTGGCCGCGCTGGCGGTGATCGGGGCCGCGACATATTGGTTTCGCAGCGCGG
GGAAAGCGCCCGACAACCGG

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MGRVWILLAAAALPATLPAILAAAPVELSLKRAVQLAVSPEGNTRVQLSGEALKQAQSRSLESRAALLPDLSAAYTQQNM
TRNLGAMGIHLTSPFPGFQIPQFVGPFTTLDGRVSATQSVFDISAYRRYQASKAAVGAAQGDLKSSGEQVAALVARAYLA
ALRADADVETARANVALSEAVRKQAEDQKAAGTGTGIEITRTRVQLANDRQRLLVAENARRSAALQLLRAIGMRLDTELV
LTDKLEATPVDAMTIQQAKAMALESRPDYQAQREREATARISADAVKLERLPTVAAFGDYGESGTGFNSSLPTRTIGITV
RIPVFDGGRREAWRAESASQARSEKVKTSDLKEQIELDVRLALDALRSAEEQVKVSREGLELAENEVTQARRRYDAGVAA
GLEVTDAQTRLERARDNQTAALYNYNVARIDLAQSVGAVRRMVE

Sequences:

>Translated_444_residues
MGRVWILLAAAALPATLPAILAAAPVELSLKRAVQLAVSPEGNTRVQLSGEALKQAQSRSLESRAALLPDLSAAYTQQNM
TRNLGAMGIHLTSPFPGFQIPQFVGPFTTLDGRVSATQSVFDISAYRRYQASKAAVGAAQGDLKSSGEQVAALVARAYLA
ALRADADVETARANVALSEAVRKQAEDQKAAGTGTGIEITRTRVQLANDRQRLLVAENARRSAALQLLRAIGMRLDTELV
LTDKLEATPVDAMTIQQAKAMALESRPDYQAQREREATARISADAVKLERLPTVAAFGDYGESGTGFNSSLPTRTIGITV
RIPVFDGGRREAWRAESASQARSEKVKTSDLKEQIELDVRLALDALRSAEEQVKVSREGLELAENEVTQARRRYDAGVAA
GLEVTDAQTRLERARDNQTAALYNYNVARIDLAQSVGAVRRMVE
>Mature_443_residues
GRVWILLAAAALPATLPAILAAAPVELSLKRAVQLAVSPEGNTRVQLSGEALKQAQSRSLESRAALLPDLSAAYTQQNMT
RNLGAMGIHLTSPFPGFQIPQFVGPFTTLDGRVSATQSVFDISAYRRYQASKAAVGAAQGDLKSSGEQVAALVARAYLAA
LRADADVETARANVALSEAVRKQAEDQKAAGTGTGIEITRTRVQLANDRQRLLVAENARRSAALQLLRAIGMRLDTELVL
TDKLEATPVDAMTIQQAKAMALESRPDYQAQREREATARISADAVKLERLPTVAAFGDYGESGTGFNSSLPTRTIGITVR
IPVFDGGRREAWRAESASQARSEKVKTSDLKEQIELDVRLALDALRSAEEQVKVSREGLELAENEVTQARRRYDAGVAAG
LEVTDAQTRLERARDNQTAALYNYNVARIDLAQSVGAVRRMVE

Specific function: Involved in the secretion of alkaline protease [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

None

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 47881; Mature: 47750

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRVWILLAAAALPATLPAILAAAPVELSLKRAVQLAVSPEGNTRVQLSGEALKQAQSRS
CCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEECCCCCCEEEECHHHHHHHHHHC
LESRAALLPDLSAAYTQQNMTRNLGAMGIHLTSPFPGFQIPQFVGPFTTLDGRVSATQSV
HHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCHHHHHHH
FDISAYRRYQASKAAVGAAQGDLKSSGEQVAALVARAYLAALRADADVETARANVALSEA
HHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VRKQAEDQKAAGTGTGIEITRTRVQLANDRQRLLVAENARRSAALQLLRAIGMRLDTELV
HHHHHHHHHCCCCCCCEEEEEHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEE
LTDKLEATPVDAMTIQQAKAMALESRPDYQAQREREATARISADAVKLERLPTVAAFGDY
EECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
GESGTGFNSSLPTRTIGITVRIPVFDGGRREAWRAESASQARSEKVKTSDLKEQIELDVR
CCCCCCCCCCCCCEEEEEEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LALDALRSAEEQVKVSREGLELAENEVTQARRRYDAGVAAGLEVTDAQTRLERARDNQTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEECHHHHHHHHHCCCCCE
ALYNYNVARIDLAQSVGAVRRMVE
EEEECCEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GRVWILLAAAALPATLPAILAAAPVELSLKRAVQLAVSPEGNTRVQLSGEALKQAQSRS
CEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEECCCCCCEEEECHHHHHHHHHHC
LESRAALLPDLSAAYTQQNMTRNLGAMGIHLTSPFPGFQIPQFVGPFTTLDGRVSATQSV
HHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCHHHHHHH
FDISAYRRYQASKAAVGAAQGDLKSSGEQVAALVARAYLAALRADADVETARANVALSEA
HHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VRKQAEDQKAAGTGTGIEITRTRVQLANDRQRLLVAENARRSAALQLLRAIGMRLDTELV
HHHHHHHHHCCCCCCCEEEEEHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEE
LTDKLEATPVDAMTIQQAKAMALESRPDYQAQREREATARISADAVKLERLPTVAAFGDY
EECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
GESGTGFNSSLPTRTIGITVRIPVFDGGRREAWRAESASQARSEKVKTSDLKEQIELDVR
CCCCCCCCCCCCCEEEEEEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LALDALRSAEEQVKVSREGLELAENEVTQARRRYDAGVAAGLEVTDAQTRLERARDNQTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEECHHHHHHHHHCCCCCE
ALYNYNVARIDLAQSVGAVRRMVE
EEEECCEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1427098; 10984043 [H]