Click here to switch to the map view.

The map label for this gene is srpG [H]

Identifier: 116624955

GI number: 116624955

Start: 7376924

End: 7377883

Strand: Direct

Name: srpG [H]

Synonym: Acid_5884

Alternate gene names: 116624955

Gene position: 7376924-7377883 (Clockwise)

Preceding gene: 116624954

Following gene: 116624956

Centisome position: 74.02

GC content: 60.42

Gene sequence:

>960_bases
TTGCGCGTCTACGACGACAATTCGCTCAGCATTGGCCGCACCCCGCTGGTTCGTCTGAACCGCGTCATCGATGGCGGAAA
AGCCATTGTTCTCGCCAAGATCGAAGGTCGCAACCCGGCCTACAGCGTCAAATGCCGCATCGGCGCCGCCATGGTCTGGG
ATGCCGAAAAACGCGGCGTTCTCCACGCCGGCAAAGAGCTGGTGGAACCCACGAGCGGCAACACCGGCATTGCTCTCGCC
TTCGTCGCCGCCGCCCGCGGCTACCCCATCCACCTCACCATGCCCGAAACCATGTCGGTGGAGCGGCGCAAGGTACTGAA
AGCCCTCGGCGCCAACCTCATTCTGACCCCCGGCTCCGACGGCATGGCCGGCTCTATCGCCAAGGCAGAGGAGATGGTGG
CCGGCGATCCCCGCCGTTATATTCTCCTGCAACAATTCAAAAATCCCGCCAACCCTGAAATCCATTTCCGCACTACCGGA
CCCGAAATCTGGGAAGACACCGAAGGTACCATCGACGTCCTCGTCTCCGGCATCGGCACCGGCGGCACCATCAGCGGCGT
CTCCCGTTACATAAAGAAAGATCGCGGACGCAAGATACTCAGCGTGGGAGTAGAACCATTCAGCAGTCAGGTACTTACCC
AGCACATGGCGGGTAAGCCCCTGAAACCCGGCCCCCACAAGATTCAAGGCATCGGCGCAGGCTTCATTCCGGATACGCTG
GATTTGACTATGGTGGACCGCGTCGAGCTGATCAGCAACGAGGAATCCATCGAAATGGCACGCCGCCTCGCTAAGGAGGA
AGGTATCTTGAGTGGTATCTCCTGCGGCGCCGCCGCCGCGGTCGCCGTTCGCCTTGGCAAGCTGCCGGAGTTCGCCGGCA
AAGTGATCGTCACCGTACTGCCAGACGCCGCGGAACGTTATCTGAGCACGACGTTATTTGAAGGTATGTTCGACGAGTGA

Upstream 100 bases:

>100_bases
CCGCCGTCAGCAGCGTCATCGATAAAACCACCTTCGCCGACTTGCAGCGCGCGTGGCAGGAACAACAGAACAAATACGTC
TTGAATTGGGAGATTTAACT

Downstream 100 bases:

>100_bases
GAGTGTGTAGCGGGCGCGTTTGGGTGATAGGGGTGAGTGCGCCCGCTGTCGTAACGGGGATTTAATGAGAAGACGAGGTC
TTTCTGCGAGACGTTACACC

Product: cysteine synthase

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRVYDDNSLSIGRTPLVRLNRVIDGGKAIVLAKIEGRNPAYSVKCRIGAAMVWDAEKRGVLHAGKELVEPTSGNTGIALA
FVAAARGYPIHLTMPETMSVERRKVLKALGANLILTPGSDGMAGSIAKAEEMVAGDPRRYILLQQFKNPANPEIHFRTTG
PEIWEDTEGTIDVLVSGIGTGGTISGVSRYIKKDRGRKILSVGVEPFSSQVLTQHMAGKPLKPGPHKIQGIGAGFIPDTL
DLTMVDRVELISNEESIEMARRLAKEEGILSGISCGAAAAVAVRLGKLPEFAGKVIVTVLPDAAERYLSTTLFEGMFDE

Sequences:

>Translated_319_residues
MRVYDDNSLSIGRTPLVRLNRVIDGGKAIVLAKIEGRNPAYSVKCRIGAAMVWDAEKRGVLHAGKELVEPTSGNTGIALA
FVAAARGYPIHLTMPETMSVERRKVLKALGANLILTPGSDGMAGSIAKAEEMVAGDPRRYILLQQFKNPANPEIHFRTTG
PEIWEDTEGTIDVLVSGIGTGGTISGVSRYIKKDRGRKILSVGVEPFSSQVLTQHMAGKPLKPGPHKIQGIGAGFIPDTL
DLTMVDRVELISNEESIEMARRLAKEEGILSGISCGAAAAVAVRLGKLPEFAGKVIVTVLPDAAERYLSTTLFEGMFDE
>Mature_319_residues
MRVYDDNSLSIGRTPLVRLNRVIDGGKAIVLAKIEGRNPAYSVKCRIGAAMVWDAEKRGVLHAGKELVEPTSGNTGIALA
FVAAARGYPIHLTMPETMSVERRKVLKALGANLILTPGSDGMAGSIAKAEEMVAGDPRRYILLQQFKNPANPEIHFRTTG
PEIWEDTEGTIDVLVSGIGTGGTISGVSRYIKKDRGRKILSVGVEPFSSQVLTQHMAGKPLKPGPHKIQGIGAGFIPDTL
DLTMVDRVELISNEESIEMARRLAKEEGILSGISCGAAAAVAVRLGKLPEFAGKVIVTVLPDAAERYLSTTLFEGMFDE

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=309, Percent_Identity=40.7766990291262, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI295821200, Length=309, Percent_Identity=40.7766990291262, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI4557415, Length=309, Percent_Identity=40.7766990291262, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI1788754, Length=319, Percent_Identity=65.5172413793103, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI2367138, Length=305, Percent_Identity=41.9672131147541, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17535051, Length=310, Percent_Identity=47.741935483871, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17562970, Length=315, Percent_Identity=46.984126984127, Blast_Score=265, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI115535073, Length=305, Percent_Identity=51.4754098360656, Blast_Score=260, Evalue=9e-70,
Organism=Caenorhabditis elegans, GI32566674, Length=305, Percent_Identity=47.8688524590164, Blast_Score=258, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17561720, Length=315, Percent_Identity=47.3015873015873, Blast_Score=242, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI32566672, Length=226, Percent_Identity=46.4601769911504, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17534315, Length=319, Percent_Identity=39.8119122257053, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25147552, Length=329, Percent_Identity=36.7781155015198, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17561716, Length=131, Percent_Identity=45.0381679389313, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=38.4126984126984, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321449, Length=322, Percent_Identity=32.2981366459627, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24643623, Length=313, Percent_Identity=37.6996805111821, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI20129101, Length=313, Percent_Identity=37.6996805111821, Blast_Score=168, Evalue=4e-42,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34187; Mature: 34187

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVYDDNSLSIGRTPLVRLNRVIDGGKAIVLAKIEGRNPAYSVKCRIGAAMVWDAEKRGV
CEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECEEEEECCCCCCH
LHAGKELVEPTSGNTGIALAFVAAARGYPIHLTMPETMSVERRKVLKALGANLILTPGSD
HHHCHHHCCCCCCCCCEEEEEEHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEECCCC
GMAGSIAKAEEMVAGDPRRYILLQQFKNPANPEIHFRTTGPEIWEDTEGTIDVLVSGIGT
CCCCCHHHHHHHHCCCCHHEEEHHHCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCC
GGTISGVSRYIKKDRGRKILSVGVEPFSSQVLTQHMAGKPLKPGPHKIQGIGAGFIPDTL
CCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCC
DLTMVDRVELISNEESIEMARRLAKEEGILSGISCGAAAAVAVRLGKLPEFAGKVIVTVL
CCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCHHHCCCEEEEEC
PDAAERYLSTTLFEGMFDE
CHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVYDDNSLSIGRTPLVRLNRVIDGGKAIVLAKIEGRNPAYSVKCRIGAAMVWDAEKRGV
CEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECEEEEECCCCCCH
LHAGKELVEPTSGNTGIALAFVAAARGYPIHLTMPETMSVERRKVLKALGANLILTPGSD
HHHCHHHCCCCCCCCCEEEEEEHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEECCCC
GMAGSIAKAEEMVAGDPRRYILLQQFKNPANPEIHFRTTGPEIWEDTEGTIDVLVSGIGT
CCCCCHHHHHHHHCCCCHHEEEHHHCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCC
GGTISGVSRYIKKDRGRKILSVGVEPFSSQVLTQHMAGKPLKPGPHKIQGIGAGFIPDTL
CCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCC
DLTMVDRVELISNEESIEMARRLAKEEGILSGISCGAAAAVAVRLGKLPEFAGKVIVTVL
CCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCHHHCCCEEEEEC
PDAAERYLSTTLFEGMFDE
CHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7603442 [H]