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The map label for this gene is fixL [H]

Identifier: 116624907

GI number: 116624907

Start: 7306125

End: 7307519

Strand: Direct

Name: fixL [H]

Synonym: Acid_5833

Alternate gene names: 116624907

Gene position: 7306125-7307519 (Clockwise)

Preceding gene: 116624899

Following gene: 116624908

Centisome position: 73.31

GC content: 58.28

Gene sequence:

>1395_bases
ATGAGTGGATCCGCACGTGCGAAGGTCACCGGCGCGGAGGAGCGCTTCCGCCTCCTTTTGGAAGCCGCCCCGGATGCCGT
GGTGGTAACGAAGCAGAGTGGAAAGATCGTCCTGGTCAATAGCCAGACCGAGAGGCTGTTTGGCTATGGCCGCGAAGAGC
TGATAGGGCGAATGGTGGACGTATTGCTGCCTCAGCGCGTGCAGCGCCGCCACGTGAAAAGCCGTGCAGCTTACATTCAG
CATCCGGCCGTGAGGCCCATGGGTACAGGCGTTGAATTGTACGCCCGGCGCAAGGATGGCACGGAGTTTCCGATCGACGT
CAGCCTCAGCCCTGTGGAAACGGGAGGGGAGGTTCTGATTTCCAGCTCCATTCGCGACATCACCGAGCGCAAGAGGTCCG
AAGAGTTGGTCTCGCATCTGGCTGCAATTGTCGCGGCATCCGACGATGCCATAATCGGCCGGTCACTCAATGGCACGATA
GTGAGTTGGAACTCCGGCGCCGAAAGGCTCTACGGGTACCAGGCGGGAGAGATGATCGGGCAATCCGGCTCAGCGCTTTT
GCCACCGGGTAAGACAGACGAAACGGCACAATTCACCAAAGTGTTGCGGCATGGGGATCACATCGAACACTACGAAACTA
CGCGGCTACACAAGGACGGGCACCCTATCGAAGTTTCGGTAACGGTGTCACCGGTCAAAGGCAGCGCGGGCACGGTGATC
GGCAGTTCGGTGATTGGCCGTGATATTACGAAGCAAAAGACGGCTGAAGAGGCCCTGCGCCAAAGCGAGGAACGTTTCCG
GCTGGCTCTCAAACATGCGCCTATCGCGGTATTCAACCAGGACAAGCAATTACGGTATACGTGGATCAATTCGCCCGCCC
TGGCGTGGGGAAAACAAGAATATCTCGGCCATACCGACGCGGAAATATTCGGCGGCGAAGAAGGCGCCCGGCTCACAGCC
ATCAAGCGGGAAGTACTGCGGAGCGGAATTGGGACGCGCACCGAGATTGAGGCCACGTCCCAAGGCGAGACGTACTATTT
GGACATGGTAGTGGAACCGCTCCGCGACGTACGAGGGACTCTGGCGGGTCTTACGTGCTCGGCCAGCGACATCACTCACA
CGAAGAAGAGCCTCCTGGAAAGGGAACGCCTGGTCGCACAACTGCAAGAGGCGCTGGAAGAGGTGAAACGGCTGAGCGGG
CTACTGTCCATTTGTGCCGGCTGCAAGAAAATCACGAATGAGCGCGGAGACTGGGAACCCCTGGAAAGCTATCTGCAGGC
GCATTCGGAGGCGAAATTCTCGCATGGGTTGTGTCCGGAATGCCTGCGAAAACTGTACCCTGAACAATTTCGCGCATGGG
AACAGTCTCAATCGAAGGACCGAACCAAGAAGTGA

Upstream 100 bases:

>100_bases
GCCAAAGGTTCATGTGATTGTACATACAAAGGGAAGGGCGGTGGGTTGCGAATTGGGGTGAATTCGATCGACCGGGGGAA
CGGAGTCGAATCCAACAGGC

Downstream 100 bases:

>100_bases
CGGGTAGCCGCGTTGGATGAAAGTGGCGTGCGCGTTGAAGTTCTTTCTGATCCGCGGGCCATGTACGGTGAGCAACAGTG
CGAGAGGAACAGGCCATAAA

Product: putative PAS/PAC sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSGSARAKVTGAEERFRLLLEAAPDAVVVTKQSGKIVLVNSQTERLFGYGREELIGRMVDVLLPQRVQRRHVKSRAAYIQ
HPAVRPMGTGVELYARRKDGTEFPIDVSLSPVETGGEVLISSSIRDITERKRSEELVSHLAAIVAASDDAIIGRSLNGTI
VSWNSGAERLYGYQAGEMIGQSGSALLPPGKTDETAQFTKVLRHGDHIEHYETTRLHKDGHPIEVSVTVSPVKGSAGTVI
GSSVIGRDITKQKTAEEALRQSEERFRLALKHAPIAVFNQDKQLRYTWINSPALAWGKQEYLGHTDAEIFGGEEGARLTA
IKREVLRSGIGTRTEIEATSQGETYYLDMVVEPLRDVRGTLAGLTCSASDITHTKKSLLERERLVAQLQEALEEVKRLSG
LLSICAGCKKITNERGDWEPLESYLQAHSEAKFSHGLCPECLRKLYPEQFRAWEQSQSKDRTKK

Sequences:

>Translated_464_residues
MSGSARAKVTGAEERFRLLLEAAPDAVVVTKQSGKIVLVNSQTERLFGYGREELIGRMVDVLLPQRVQRRHVKSRAAYIQ
HPAVRPMGTGVELYARRKDGTEFPIDVSLSPVETGGEVLISSSIRDITERKRSEELVSHLAAIVAASDDAIIGRSLNGTI
VSWNSGAERLYGYQAGEMIGQSGSALLPPGKTDETAQFTKVLRHGDHIEHYETTRLHKDGHPIEVSVTVSPVKGSAGTVI
GSSVIGRDITKQKTAEEALRQSEERFRLALKHAPIAVFNQDKQLRYTWINSPALAWGKQEYLGHTDAEIFGGEEGARLTA
IKREVLRSGIGTRTEIEATSQGETYYLDMVVEPLRDVRGTLAGLTCSASDITHTKKSLLERERLVAQLQEALEEVKRLSG
LLSICAGCKKITNERGDWEPLESYLQAHSEAKFSHGLCPECLRKLYPEQFRAWEQSQSKDRTKK
>Mature_463_residues
SGSARAKVTGAEERFRLLLEAAPDAVVVTKQSGKIVLVNSQTERLFGYGREELIGRMVDVLLPQRVQRRHVKSRAAYIQH
PAVRPMGTGVELYARRKDGTEFPIDVSLSPVETGGEVLISSSIRDITERKRSEELVSHLAAIVAASDDAIIGRSLNGTIV
SWNSGAERLYGYQAGEMIGQSGSALLPPGKTDETAQFTKVLRHGDHIEHYETTRLHKDGHPIEVSVTVSPVKGSAGTVIG
SSVIGRDITKQKTAEEALRQSEERFRLALKHAPIAVFNQDKQLRYTWINSPALAWGKQEYLGHTDAEIFGGEEGARLTAI
KREVLRSGIGTRTEIEATSQGETYYLDMVVEPLRDVRGTLAGLTCSASDITHTKKSLLERERLVAQLQEALEEVKRLSGL
LSICAGCKKITNERGDWEPLESYLQAHSEAKFSHGLCPECLRKLYPEQFRAWEQSQSKDRTKK

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=271, Percent_Identity=25.4612546125461, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51354; Mature: 51223

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50112 PAS ; PS50113 PAC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSARAKVTGAEERFRLLLEAAPDAVVVTKQSGKIVLVNSQTERLFGYGREELIGRMVD
CCCCCCEEECCHHHHHHHHHHCCCCEEEEEECCCCEEEEECCHHHHHCCCHHHHHHHHHH
VLLPQRVQRRHVKSRAAYIQHPAVRPMGTGVELYARRKDGTEFPIDVSLSPVETGGEVLI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCEEE
SSSIRDITERKRSEELVSHLAAIVAASDDAIIGRSLNGTIVSWNSGAERLYGYQAGEMIG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEEECCCHHHHHCCCHHHHHC
QSGSALLPPGKTDETAQFTKVLRHGDHIEHYETTRLHKDGHPIEVSVTVSPVKGSAGTVI
CCCCEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCH
GSSVIGRDITKQKTAEEALRQSEERFRLALKHAPIAVFNQDKQLRYTWINSPALAWGKQE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEECCCHHHCCCHH
YLGHTDAEIFGGEEGARLTAIKREVLRSGIGTRTEIEATSQGETYYLDMVVEPLRDVRGT
HCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHH
LAGLTCSASDITHTKKSLLERERLVAQLQEALEEVKRLSGLLSICAGCKKITNERGDWEP
HHCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LESYLQAHSEAKFSHGLCPECLRKLYPEQFRAWEQSQSKDRTKK
HHHHHHHHHHCHHCCCCCHHHHHHHCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SGSARAKVTGAEERFRLLLEAAPDAVVVTKQSGKIVLVNSQTERLFGYGREELIGRMVD
CCCCCEEECCHHHHHHHHHHCCCCEEEEEECCCCEEEEECCHHHHHCCCHHHHHHHHHH
VLLPQRVQRRHVKSRAAYIQHPAVRPMGTGVELYARRKDGTEFPIDVSLSPVETGGEVLI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCEEE
SSSIRDITERKRSEELVSHLAAIVAASDDAIIGRSLNGTIVSWNSGAERLYGYQAGEMIG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEEECCCHHHHHCCCHHHHHC
QSGSALLPPGKTDETAQFTKVLRHGDHIEHYETTRLHKDGHPIEVSVTVSPVKGSAGTVI
CCCCEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCH
GSSVIGRDITKQKTAEEALRQSEERFRLALKHAPIAVFNQDKQLRYTWINSPALAWGKQE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEECCCHHHCCCHH
YLGHTDAEIFGGEEGARLTAIKREVLRSGIGTRTEIEATSQGETYYLDMVVEPLRDVRGT
HCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHH
LAGLTCSASDITHTKKSLLERERLVAQLQEALEEVKRLSGLLSICAGCKKITNERGDWEP
HHCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LESYLQAHSEAKFSHGLCPECLRKLYPEQFRAWEQSQSKDRTKK
HHHHHHHHHHCHHCCCCCHHHHHHHCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]