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The map label for this gene is trpB2 [H]

Identifier: 116624824

GI number: 116624824

Start: 7199513

End: 7200871

Strand: Reverse

Name: trpB2 [H]

Synonym: Acid_5748

Alternate gene names: 116624824

Gene position: 7200871-7199513 (Counterclockwise)

Preceding gene: 116624826

Following gene: 116624823

Centisome position: 72.26

GC content: 64.09

Gene sequence:

>1359_bases
ATGTCCGAGCATTCCAAGTTTCTGCTTGCGGATAGCCAGATTCCCACCGCGTGGGTCAATGTTCTGCCGTCGTTGCCGGA
ACCGCTTGATCCGCCTCTCCATCCCGCCACGCGCGAGCCGCTGACACCGCAGGATCTGCTGCCGATCTTTCCAATGGAGC
TGATCGAGCAGGAATTCTCGCCGCAACCGCTGATCGATATTCCGGGAGAAGTGATGGATATCTACCGGCTGTGGCGGGCG
ACTCCGCTGTATCGCGCGCGGCGGCTGGAAAAGGCGCTGGATACTCCAGCCCACATCTATTACAAGTACGAGGGCACCAG
TCCGGCCGGGAGCCACAAGCCCAATACGGCGGTGGCGCAGGCGTATTACAACAAGCGCGCGGGGATCAAGAGGCTGGCGA
CGGAGACCGGGGCGGGGCAGTGGGGCAGCGCGCTTTCGCTGGCTTGTAAGTTGTTCGGCCTCGAGTGCACGGTCTACATG
GTCCGCTGCAGCTACGACCAGAAGCCGTACCGGCGGATGATGATGCACGTGTGGGGCGGAGAGGTCTTCGCGAGTCCGAG
CGAGCACACGCATGCCGGCCGCGCGGTGCTGGCGCAGACCCCCGATTCACCGGGCAGCCTGGGCATCGCGATCAGCGAGG
CGGTGGAGGACGCGGCCACGCATGGCGACACCAACTACGCACTGGGCAGCGTGCTCAACCACGTGATGCTGCACCAGACC
GTGATCGGGCAGGAGGCGAAACTGCAGATGGCCCTGGCGGGTGAGGAGCCGGAGGTCGTGATCGGCTGTTTTGGCGGCGG
CAGCAACTTCGGCGGACTCGCGCTGCCATACGTGCTGGACCGGCTGGCCGGCAAGGGACCGCGCATCGTGGCGGTGGAAC
CGGCGGCCTGCCCCAGCCTGACCAAGGGCCGGCTGGCGTATGACTACGGCGACACTGCTAAACTTACCCCGCTGATTAAG
ATGTACACGCTGGGGCACGATTTTGTGCCGCCCGGGATTCACGCGGGTGGATTGCGGTATCACGGCGCCTCTCCTTTGCT
AAGCCACCTGCTGAAACTGGGCCAGATCGAGGCGCGCTCCTATACCCAGGTACCGGTTTTCGACGCCGCACTGCAATTTG
CGCAAACCGAGGGCATTTTACCGGCGCCGGAATCCTCGCATGCCATTCGAGCGGCCGTGGACGAGGCGCTGGCGGCGCGG
GAAGAGGGCAAGGCGCGCACCATCCTCTTCTGTCTGAGCGGCCACGGGCATTTCGACCTAAGCGCGTACGAGAATTACCT
CACCGGCAAGCTGCAGGACTACGAGTACCCCGGGCAGGATGTGGCCAGGGCTATGGAGGGAATTCCGGCCGGAAGATGA

Upstream 100 bases:

>100_bases
CACTTCCGTGCGGACGGGATGCCAGGTAAGAAGCAAGGCCGGGCAACCGCTGGAAGGGCCGGGAGGCCGGTCCTACGGTT
TGTTATAGTAGGCCTTCCCT

Downstream 100 bases:

>100_bases
GCGATTCGCAGCCGGAAGGCGGGTCAAACCAAAGAAAAGGTTAGCGTTCACATTCTTCCGCCTAACGCTACCCTCAAATG
AGGGGGTACTGGTTTGTAAG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSEHSKFLLADSQIPTAWVNVLPSLPEPLDPPLHPATREPLTPQDLLPIFPMELIEQEFSPQPLIDIPGEVMDIYRLWRA
TPLYRARRLEKALDTPAHIYYKYEGTSPAGSHKPNTAVAQAYYNKRAGIKRLATETGAGQWGSALSLACKLFGLECTVYM
VRCSYDQKPYRRMMMHVWGGEVFASPSEHTHAGRAVLAQTPDSPGSLGIAISEAVEDAATHGDTNYALGSVLNHVMLHQT
VIGQEAKLQMALAGEEPEVVIGCFGGGSNFGGLALPYVLDRLAGKGPRIVAVEPAACPSLTKGRLAYDYGDTAKLTPLIK
MYTLGHDFVPPGIHAGGLRYHGASPLLSHLLKLGQIEARSYTQVPVFDAALQFAQTEGILPAPESSHAIRAAVDEALAAR
EEGKARTILFCLSGHGHFDLSAYENYLTGKLQDYEYPGQDVARAMEGIPAGR

Sequences:

>Translated_452_residues
MSEHSKFLLADSQIPTAWVNVLPSLPEPLDPPLHPATREPLTPQDLLPIFPMELIEQEFSPQPLIDIPGEVMDIYRLWRA
TPLYRARRLEKALDTPAHIYYKYEGTSPAGSHKPNTAVAQAYYNKRAGIKRLATETGAGQWGSALSLACKLFGLECTVYM
VRCSYDQKPYRRMMMHVWGGEVFASPSEHTHAGRAVLAQTPDSPGSLGIAISEAVEDAATHGDTNYALGSVLNHVMLHQT
VIGQEAKLQMALAGEEPEVVIGCFGGGSNFGGLALPYVLDRLAGKGPRIVAVEPAACPSLTKGRLAYDYGDTAKLTPLIK
MYTLGHDFVPPGIHAGGLRYHGASPLLSHLLKLGQIEARSYTQVPVFDAALQFAQTEGILPAPESSHAIRAAVDEALAAR
EEGKARTILFCLSGHGHFDLSAYENYLTGKLQDYEYPGQDVARAMEGIPAGR
>Mature_451_residues
SEHSKFLLADSQIPTAWVNVLPSLPEPLDPPLHPATREPLTPQDLLPIFPMELIEQEFSPQPLIDIPGEVMDIYRLWRAT
PLYRARRLEKALDTPAHIYYKYEGTSPAGSHKPNTAVAQAYYNKRAGIKRLATETGAGQWGSALSLACKLFGLECTVYMV
RCSYDQKPYRRMMMHVWGGEVFASPSEHTHAGRAVLAQTPDSPGSLGIAISEAVEDAATHGDTNYALGSVLNHVMLHQTV
IGQEAKLQMALAGEEPEVVIGCFGGGSNFGGLALPYVLDRLAGKGPRIVAVEPAACPSLTKGRLAYDYGDTAKLTPLIKM
YTLGHDFVPPGIHAGGLRYHGASPLLSHLLKLGQIEARSYTQVPVFDAALQFAQTEGILPAPESSHAIRAAVDEALAARE
EGKARTILFCLSGHGHFDLSAYENYLTGKLQDYEYPGQDVARAMEGIPAGR

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=407, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6321412, Length=371, Percent_Identity=29.9191374663073, Blast_Score=119, Evalue=8e-28,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 49051; Mature: 48919

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHSKFLLADSQIPTAWVNVLPSLPEPLDPPLHPATREPLTPQDLLPIFPMELIEQEFS
CCCCCCEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHCC
PQPLIDIPGEVMDIYRLWRATPLYRARRLEKALDTPAHIYYKYEGTSPAGSHKPNTAVAQ
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHH
AYYNKRAGIKRLATETGAGQWGSALSLACKLFGLECTVYMVRCSYDQKPYRRMMMHVWGG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHCCC
EVFASPSEHTHAGRAVLAQTPDSPGSLGIAISEAVEDAATHGDTNYALGSVLNHVMLHQT
CEECCCCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VIGQEAKLQMALAGEEPEVVIGCFGGGSNFGGLALPYVLDRLAGKGPRIVAVEPAACPSL
HHCCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCC
TKGRLAYDYGDTAKLTPLIKMYTLGHDFVPPGIHAGGLRYHGASPLLSHLLKLGQIEARS
CCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCC
YTQVPVFDAALQFAQTEGILPAPESSHAIRAAVDEALAAREEGKARTILFCLSGHGHFDL
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEH
SAYENYLTGKLQDYEYPGQDVARAMEGIPAGR
HHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
SEHSKFLLADSQIPTAWVNVLPSLPEPLDPPLHPATREPLTPQDLLPIFPMELIEQEFS
CCCCCEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHCC
PQPLIDIPGEVMDIYRLWRATPLYRARRLEKALDTPAHIYYKYEGTSPAGSHKPNTAVAQ
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHH
AYYNKRAGIKRLATETGAGQWGSALSLACKLFGLECTVYMVRCSYDQKPYRRMMMHVWGG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHCCC
EVFASPSEHTHAGRAVLAQTPDSPGSLGIAISEAVEDAATHGDTNYALGSVLNHVMLHQT
CEECCCCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VIGQEAKLQMALAGEEPEVVIGCFGGGSNFGGLALPYVLDRLAGKGPRIVAVEPAACPSL
HHCCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCC
TKGRLAYDYGDTAKLTPLIKMYTLGHDFVPPGIHAGGLRYHGASPLLSHLLKLGQIEARS
CCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCC
YTQVPVFDAALQFAQTEGILPAPESSHAIRAAVDEALAAREEGKARTILFCLSGHGHFDL
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEH
SAYENYLTGKLQDYEYPGQDVARAMEGIPAGR
HHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]