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The map label for this gene is mls [H]

Identifier: 116624778

GI number: 116624778

Start: 7143063

End: 7144640

Strand: Direct

Name: mls [H]

Synonym: Acid_5702

Alternate gene names: 116624778

Gene position: 7143063-7144640 (Clockwise)

Preceding gene: 116624776

Following gene: 116624779

Centisome position: 71.68

GC content: 63.62

Gene sequence:

>1578_bases
ATGTTTGCCACACAAGAGAAGTCCATCCAGATCACCGCCCCCGTCACTGGCGCGCAGCAACTTATCCTCAGCGAAGAGGC
CCTCCGCTTTCTCGCCAGGCTGGCGCGCTCGGTCGAACCCCGCCGCCGTCAACTGCTCGATGACCGTGAGCGGCGCCAGC
AGGAAATCGATGCCGGCGCCCTGCCCGAATTTCTCCCCGCCACCGCGCGCATCCGCGCCCGCGAGTGGTACGTGGCGCCC
ATCCCCGCCGACCTCCAGGATCGCCGCGTGGAAATCACCGGCCCGGTCGATCGTAAGATGATCATCAACGCCCTCAACTC
CGGCGCCAATGTCTTCATGGCGGATTTTGAGGATTCCAACTCGCCCACCTGGGGCAACGTGATGCAGGGCCAGGTAAACC
TCCGCGACGCCGTACGCGGCGTCATCACCTACACCAGCCCCGAAGGCAAGCGCTACGAGCTCTGTGAAACGCCCGCAGTC
CTCATGGTCCGCCCGCGGGGCTGGCACCTGGTTGAGAAGCATGTCCTGGTGGATGGCGAACCGATCTCCGCCTCCCTCTT
CGATTTTGGCCTCTTCTTCTTCCACAACGCCCGGACGCTGATCGCCAACGGCACCGGCCCGTATTTCTACCTCCCGAAAA
TGGAGAGCCATCTCGAAGCCCGCCTCTGGAATGAGGTGTTCATCCTCGCCCAGCAGGAACTCGGCATCTCGCGCGGCACC
ATCCGCGCCACCGCCCTTATCGAGACCATCTTCGCCGCCTTCGAAATGGACGAGATCCTCTGGGAACTCCGCGAGCACTC
GGCCGGCCTGAATTGCGGCCGCTGGGACTACATCTTCAGCTTCATCAAAAAATTCCGCAACCACTCCGGCTTCGTGCTGC
CGGACCGCGTCCAGGTCGGCATGGACCGCCATTTCCTGCGCTCCTATGCCGATCTGCTGATCCACACCTGCCATCGCCGC
GGCGCGCATGCCATGGGCGGCATGGCCGCGCAGATCCCCATCAAAAACGATCCCGAGGCCAATGCCGCCGCGCTCGAAAA
GGTGCGGCTCGATAAGCTTCGCGAAGTTTGGGCCGGCCACGATGGCACCTGGGTTGCCCATCCCGCCCTCGTATCGCTCG
CCCGCGATGTCTTCGACACCTACATGCCGTTCCCCAACCAGATCGATAAGAAGACGTCCATCGACGTCAACCCCGCCGAC
CTGCTGACCGTCCCCACCGGCGAGATCACCCTCGAAGGCCTGAGCCGCAATATCGATATCGGCCTCCAGTACCTCACGAC
CTGGCTGTGCGGCAACGGCTGCGTTCCCATCTATAACCTGATGGAGGATGCCGCCACCGCCGAAATCTCCCGCGCCCAGG
TCTGGCAGTGGCTGACCCACGGCGCGCACCTCTCGAATGGCCAGCGCGTCACCCCGGTCCTCGTACGCGAAACCCTGGAG
CACCACCGCCGCCGCCTCGAATCCCAATTCCCGGCGCGCGAACTCTCCCATGCCGTCCGCCTCTTCGAGACCCTGATCAT
CAACCCCGAATTCACCGATTTCCTGACCGCGCCGGCCTACGACCTGTTAGATGCGTAA

Upstream 100 bases:

>100_bases
AAATGCGCCAACCGGCAATCGCGCTGGGACGAATCGGACCAGTCGTCCGGAGCGGACCAGCGCGCATCGCTTTTTCGCAA
TTCCACGCTACATTGCTCAC

Downstream 100 bases:

>100_bases
TCTCTTGCCGAGAAGGCACGGCTCACCGCATGGTATTTACCGTGCATTTGTCTGCTTGCAGCCCGCGAAGATTTCTCCAA
TCAGCTTGGATGCCCTCTTT

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MFATQEKSIQITAPVTGAQQLILSEEALRFLARLARSVEPRRRQLLDDRERRQQEIDAGALPEFLPATARIRAREWYVAP
IPADLQDRRVEITGPVDRKMIINALNSGANVFMADFEDSNSPTWGNVMQGQVNLRDAVRGVITYTSPEGKRYELCETPAV
LMVRPRGWHLVEKHVLVDGEPISASLFDFGLFFFHNARTLIANGTGPYFYLPKMESHLEARLWNEVFILAQQELGISRGT
IRATALIETIFAAFEMDEILWELREHSAGLNCGRWDYIFSFIKKFRNHSGFVLPDRVQVGMDRHFLRSYADLLIHTCHRR
GAHAMGGMAAQIPIKNDPEANAAALEKVRLDKLREVWAGHDGTWVAHPALVSLARDVFDTYMPFPNQIDKKTSIDVNPAD
LLTVPTGEITLEGLSRNIDIGLQYLTTWLCGNGCVPIYNLMEDAATAEISRAQVWQWLTHGAHLSNGQRVTPVLVRETLE
HHRRRLESQFPARELSHAVRLFETLIINPEFTDFLTAPAYDLLDA

Sequences:

>Translated_525_residues
MFATQEKSIQITAPVTGAQQLILSEEALRFLARLARSVEPRRRQLLDDRERRQQEIDAGALPEFLPATARIRAREWYVAP
IPADLQDRRVEITGPVDRKMIINALNSGANVFMADFEDSNSPTWGNVMQGQVNLRDAVRGVITYTSPEGKRYELCETPAV
LMVRPRGWHLVEKHVLVDGEPISASLFDFGLFFFHNARTLIANGTGPYFYLPKMESHLEARLWNEVFILAQQELGISRGT
IRATALIETIFAAFEMDEILWELREHSAGLNCGRWDYIFSFIKKFRNHSGFVLPDRVQVGMDRHFLRSYADLLIHTCHRR
GAHAMGGMAAQIPIKNDPEANAAALEKVRLDKLREVWAGHDGTWVAHPALVSLARDVFDTYMPFPNQIDKKTSIDVNPAD
LLTVPTGEITLEGLSRNIDIGLQYLTTWLCGNGCVPIYNLMEDAATAEISRAQVWQWLTHGAHLSNGQRVTPVLVRETLE
HHRRRLESQFPARELSHAVRLFETLIINPEFTDFLTAPAYDLLDA
>Mature_525_residues
MFATQEKSIQITAPVTGAQQLILSEEALRFLARLARSVEPRRRQLLDDRERRQQEIDAGALPEFLPATARIRAREWYVAP
IPADLQDRRVEITGPVDRKMIINALNSGANVFMADFEDSNSPTWGNVMQGQVNLRDAVRGVITYTSPEGKRYELCETPAV
LMVRPRGWHLVEKHVLVDGEPISASLFDFGLFFFHNARTLIANGTGPYFYLPKMESHLEARLWNEVFILAQQELGISRGT
IRATALIETIFAAFEMDEILWELREHSAGLNCGRWDYIFSFIKKFRNHSGFVLPDRVQVGMDRHFLRSYADLLIHTCHRR
GAHAMGGMAAQIPIKNDPEANAAALEKVRLDKLREVWAGHDGTWVAHPALVSLARDVFDTYMPFPNQIDKKTSIDVNPAD
LLTVPTGEITLEGLSRNIDIGLQYLTTWLCGNGCVPIYNLMEDAATAEISRAQVWQWLTHGAHLSNGQRVTPVLVRETLE
HHRRRLESQFPARELSHAVRLFETLIINPEFTDFLTAPAYDLLDA

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=529, Percent_Identity=52.5519848771267, Blast_Score=532, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17561814, Length=528, Percent_Identity=54.5454545454545, Blast_Score=568, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI71982926, Length=425, Percent_Identity=56, Blast_Score=472, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6324212, Length=511, Percent_Identity=54.2074363992172, Blast_Score=546, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6322222, Length=511, Percent_Identity=52.0547945205479, Blast_Score=524, Evalue=1e-149,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 59499; Mature: 59499

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFATQEKSIQITAPVTGAQQLILSEEALRFLARLARSVEPRRRQLLDDRERRQQEIDAGA
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
LPEFLPATARIRAREWYVAPIPADLQDRRVEITGPVDRKMIINALNSGANVFMADFEDSN
CHHHCCCHHEEEECEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCCCC
SPTWGNVMQGQVNLRDAVRGVITYTSPEGKRYELCETPAVLMVRPRGWHLVEKHVLVDGE
CCCCCCCEECCEEHHHHHCEEEEEECCCCCEEEECCCCEEEEECCCCCEEEEEEEEECCC
PISASLFDFGLFFFHNARTLIANGTGPYFYLPKMESHLEARLWNEVFILAQQELGISRGT
CCCHHHHHHHHHHHHCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHEEEHHHCCCCCCH
IRATALIETIFAAFEMDEILWELREHSAGLNCGRWDYIFSFIKKFRNHSGFVLPDRVQVG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHC
MDRHFLRSYADLLIHTCHRRGAHAMGGMAAQIPIKNDPEANAAALEKVRLDKLREVWAGH
CCHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCC
DGTWVAHPALVSLARDVFDTYMPFPNQIDKKTSIDVNPADLLTVPTGEITLEGLSRNIDI
CCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEEECCCCEEEECCCCCHHH
GLQYLTTWLCGNGCVPIYNLMEDAATAEISRAQVWQWLTHGAHLSNGQRVTPVLVRETLE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHH
HHRRRLESQFPARELSHAVRLFETLIINPEFTDFLTAPAYDLLDA
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHCC
>Mature Secondary Structure
MFATQEKSIQITAPVTGAQQLILSEEALRFLARLARSVEPRRRQLLDDRERRQQEIDAGA
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
LPEFLPATARIRAREWYVAPIPADLQDRRVEITGPVDRKMIINALNSGANVFMADFEDSN
CHHHCCCHHEEEECEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCCCC
SPTWGNVMQGQVNLRDAVRGVITYTSPEGKRYELCETPAVLMVRPRGWHLVEKHVLVDGE
CCCCCCCEECCEEHHHHHCEEEEEECCCCCEEEECCCCEEEEECCCCCEEEEEEEEECCC
PISASLFDFGLFFFHNARTLIANGTGPYFYLPKMESHLEARLWNEVFILAQQELGISRGT
CCCHHHHHHHHHHHHCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHEEEHHHCCCCCCH
IRATALIETIFAAFEMDEILWELREHSAGLNCGRWDYIFSFIKKFRNHSGFVLPDRVQVG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHC
MDRHFLRSYADLLIHTCHRRGAHAMGGMAAQIPIKNDPEANAAALEKVRLDKLREVWAGH
CCHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCC
DGTWVAHPALVSLARDVFDTYMPFPNQIDKKTSIDVNPADLLTVPTGEITLEGLSRNIDI
CCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEEECCCCEEEECCCCCHHH
GLQYLTTWLCGNGCVPIYNLMEDAATAEISRAQVWQWLTHGAHLSNGQRVTPVLVRETLE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHH
HHRRRLESQFPARELSHAVRLFETLIINPEFTDFLTAPAYDLLDA
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA