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The map label for this gene is aceA [H]

Identifier: 116624777

GI number: 116624777

Start: 7141637

End: 7142929

Strand: Reverse

Name: aceA [H]

Synonym: Acid_5701

Alternate gene names: 116624777

Gene position: 7142929-7141637 (Counterclockwise)

Preceding gene: 116624789

Following gene: 116624773

Centisome position: 71.68

GC content: 65.58

Gene sequence:

>1293_bases
ATGACGAATCGTGCCGACGAGATTTTGCGGGAGTGGGAGACCGACCCGCGGTGGAGAGGAATCAAACGGCCTTACAGCGC
GGAAAATGTTCTGCGGCTGCGCGGCTCGGTGCGGGTGGAGTACACGCTGGCGCGCATGGGCGCGGAGCGGCTGTGGCATC
TGCTGCACACCGAACCCTACGTGGCGGCGCTGGGGGCGCTGACGGGAAACCAGGCGGTGCAACAGGTGCAGGCAGGGCTA
AAGGCTATCTATTTGAGCGGCTGGCAGGTAGCCGGCGACGCCAACCTGGGCGGCCAGATGTACCCGGACCAGAGCCTGTA
TCCGGCGAACAGCGTGCCGGCGGTGGTGCGGGCGATCAATAACGCGCTGCAGCGGGCCGACCAGATCCACAGCGCGGAGG
GCGCGCATGGGCACTGCTGGTTCGCGCCGATCGTGGCCGATGCGGAGGCAGGCTTCGGCGGACCGCTCAATGCTTTCGAA
CTCATGAAGGGAATGATCGAGGCGGGTGCGGGCGCGGTGCACTTCGAGGATCAGCTTTCCTCGGCGAAGAAGTGCGGGCA
CCTGGGCGGCAAGGTAGTGGTGCCGATTGCCGAATTCGTGCAGAAGCTGGTTGCAGCACGGCTGGCAGCGGACGTGATGG
GGATTCCGACGGTGCTGGTGGCGCGTACGGATGCGAATAGCGCGGGCCTGCTGCTGAGCGACATCGACTCGCGCGATATG
CCGTTTTTGAGCGGCGGGCGGACCAGCGAGGGCTTCTTCCATTTCCGCGGCGGGATTCAGGCGGCGATCGCGCGCGGCGT
GGCGTATGCGCCCTATGCGGACATGCTGTGGTGCGAGACGTCCGAGCCGAATTTGGCGGAGGCGCGGGAGTTCGCGGAGG
CGATCCACGCGCGGTTCCCCGGGAAACTGCTGGCCTACAACTGCTCGCCGAGCTTTCACTGGAGGAAGAAACTGGACGAC
GCCACGATCGGGCGCTTCCAACAGGAGCTGGGTGCGATGGGTTACAAGTTCCAGTTCGTGACGCTGGCGGGATTCCACGC
GCTCAACCTGAGCATGTTTTCCCTGGCGCGCGCGTATGCGGAGGTGGGAATGGCGGCGTACGCGAGTTTGCAGGAGCACG
AGTTCGAGGTGTCGCGCGAACATGGGTACTCGGCGGTTCGGCACCAGCGGTTCGTGGGCACGGGATACTTCGACGAAGTG
CAGCAGGTGATCTCCGGCGGACAGAGCTCCACGGCGGCGCTGGCGGGTTCGACGGAGGCGGCGCAGTTCAATGGCGTGGA
GGTGACGCCGTAG

Upstream 100 bases:

>100_bases
ATGCGCGCTGGTCCGCTCCGGACGACTGGTCCGATTCGTCCCAGCGCGATTGCCGGTTGGCGCATTTCGGCATCTACTAC
GACACAGGAGAGGAACCAAA

Downstream 100 bases:

>100_bases
CAGCAACGGACGCTCCCTCACGGTCGCGGCTCTGATTGAGGGCGACGCACTTGGGCGAGGGCTCGTTGCGCTCGTTGGCG
GCGGAGGTATCAGACTGGCG

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTNRADEILREWETDPRWRGIKRPYSAENVLRLRGSVRVEYTLARMGAERLWHLLHTEPYVAALGALTGNQAVQQVQAGL
KAIYLSGWQVAGDANLGGQMYPDQSLYPANSVPAVVRAINNALQRADQIHSAEGAHGHCWFAPIVADAEAGFGGPLNAFE
LMKGMIEAGAGAVHFEDQLSSAKKCGHLGGKVVVPIAEFVQKLVAARLAADVMGIPTVLVARTDANSAGLLLSDIDSRDM
PFLSGGRTSEGFFHFRGGIQAAIARGVAYAPYADMLWCETSEPNLAEAREFAEAIHARFPGKLLAYNCSPSFHWRKKLDD
ATIGRFQQELGAMGYKFQFVTLAGFHALNLSMFSLARAYAEVGMAAYASLQEHEFEVSREHGYSAVRHQRFVGTGYFDEV
QQVISGGQSSTAALAGSTEAAQFNGVEVTP

Sequences:

>Translated_430_residues
MTNRADEILREWETDPRWRGIKRPYSAENVLRLRGSVRVEYTLARMGAERLWHLLHTEPYVAALGALTGNQAVQQVQAGL
KAIYLSGWQVAGDANLGGQMYPDQSLYPANSVPAVVRAINNALQRADQIHSAEGAHGHCWFAPIVADAEAGFGGPLNAFE
LMKGMIEAGAGAVHFEDQLSSAKKCGHLGGKVVVPIAEFVQKLVAARLAADVMGIPTVLVARTDANSAGLLLSDIDSRDM
PFLSGGRTSEGFFHFRGGIQAAIARGVAYAPYADMLWCETSEPNLAEAREFAEAIHARFPGKLLAYNCSPSFHWRKKLDD
ATIGRFQQELGAMGYKFQFVTLAGFHALNLSMFSLARAYAEVGMAAYASLQEHEFEVSREHGYSAVRHQRFVGTGYFDEV
QQVISGGQSSTAALAGSTEAAQFNGVEVTP
>Mature_429_residues
TNRADEILREWETDPRWRGIKRPYSAENVLRLRGSVRVEYTLARMGAERLWHLLHTEPYVAALGALTGNQAVQQVQAGLK
AIYLSGWQVAGDANLGGQMYPDQSLYPANSVPAVVRAINNALQRADQIHSAEGAHGHCWFAPIVADAEAGFGGPLNAFEL
MKGMIEAGAGAVHFEDQLSSAKKCGHLGGKVVVPIAEFVQKLVAARLAADVMGIPTVLVARTDANSAGLLLSDIDSRDMP
FLSGGRTSEGFFHFRGGIQAAIARGVAYAPYADMLWCETSEPNLAEAREFAEAIHARFPGKLLAYNCSPSFHWRKKLDDA
TIGRFQQELGAMGYKFQFVTLAGFHALNLSMFSLARAYAEVGMAAYASLQEHEFEVSREHGYSAVRHQRFVGTGYFDEVQ
QVISGGQSSTAALAGSTEAAQFNGVEVTP

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=432, Percent_Identity=63.1944444444444, Blast_Score=553, Evalue=1e-158,
Organism=Escherichia coli, GI1786525, Length=261, Percent_Identity=29.5019157088123, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17561814, Length=414, Percent_Identity=61.8357487922705, Blast_Score=536, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI71982926, Length=414, Percent_Identity=61.8357487922705, Blast_Score=536, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6320908, Length=289, Percent_Identity=35.9861591695502, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325263, Length=251, Percent_Identity=34.2629482071713, Blast_Score=140, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 46625; Mature: 46494

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRADEILREWETDPRWRGIKRPYSAENVLRLRGSVRVEYTLARMGAERLWHLLHTEPY
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCHH
VAALGALTGNQAVQQVQAGLKAIYLSGWQVAGDANLGGQMYPDQSLYPANSVPAVVRAIN
HHHHHHHCCHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHH
NALQRADQIHSAEGAHGHCWFAPIVADAEAGFGGPLNAFELMKGMIEAGAGAVHFEDQLS
HHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHH
SAKKCGHLGGKVVVPIAEFVQKLVAARLAADVMGIPTVLVARTDANSAGLLLSDIDSRDM
HHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCC
PFLSGGRTSEGFFHFRGGIQAAIARGVAYAPYADMLWCETSEPNLAEAREFAEAIHARFP
CCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
GKLLAYNCSPSFHWRKKLDDATIGRFQQELGAMGYKFQFVTLAGFHALNLSMFSLARAYA
CCEEEEECCCCCHHHHHCCHHHHHHHHHHHCCCCEEEEEEEEECHHHHHHHHHHHHHHHH
EVGMAAYASLQEHEFEVSREHGYSAVRHQRFVGTGYFDEVQQVISGGQSSTAALAGSTEA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEECCCCCC
AQFNGVEVTP
CCCCCEEECC
>Mature Secondary Structure 
TNRADEILREWETDPRWRGIKRPYSAENVLRLRGSVRVEYTLARMGAERLWHLLHTEPY
CCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCHH
VAALGALTGNQAVQQVQAGLKAIYLSGWQVAGDANLGGQMYPDQSLYPANSVPAVVRAIN
HHHHHHHCCHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHH
NALQRADQIHSAEGAHGHCWFAPIVADAEAGFGGPLNAFELMKGMIEAGAGAVHFEDQLS
HHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHH
SAKKCGHLGGKVVVPIAEFVQKLVAARLAADVMGIPTVLVARTDANSAGLLLSDIDSRDM
HHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCC
PFLSGGRTSEGFFHFRGGIQAAIARGVAYAPYADMLWCETSEPNLAEAREFAEAIHARFP
CCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
GKLLAYNCSPSFHWRKKLDDATIGRFQQELGAMGYKFQFVTLAGFHALNLSMFSLARAYA
CCEEEEECCCCCHHHHHCCHHHHHHHHHHHCCCCEEEEEEEEECHHHHHHHHHHHHHHHH
EVGMAAYASLQEHEFEVSREHGYSAVRHQRFVGTGYFDEVQQVISGGQSSTAALAGSTEA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEECCCCCC
AQFNGVEVTP
CCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]