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The map label for this gene is folC [H]

Identifier: 116624736

GI number: 116624736

Start: 7093787

End: 7095055

Strand: Reverse

Name: folC [H]

Synonym: Acid_5660

Alternate gene names: 116624736

Gene position: 7095055-7093787 (Counterclockwise)

Preceding gene: 116624737

Following gene: 116624735

Centisome position: 71.2

GC content: 66.9

Gene sequence:

>1269_bases
TTGAACTACCCTGATTCGGTCCAATTCCTCTACGCGCTCGGCAACGAGGTCAAGACCGCCAAGCTCGGTCTGGAACGCAT
CGCCCGCGTGCTCGAAGCTCTTGGCAATCCGCACCGCCGGACCCGCATCGTCCACGTCGCGGGGACGAACGGCAAAGGTT
CCACCTGCGCCATGATTGAATCGGGACTGCGCGCGTCCGGGCGAAGCACGGGACTTTTCACCTCGCCCCACCTCGCCGAA
CCCACGGAGCGCATCCGCATCAACGGCGTCCCGGTCTCCGCCGAGCGCTTCTCCGCCGCCTTCGACCGCGTTCACGCCGC
CGTGGAACAACTGCTCGCCGTCAACGCCATCGATCTCCACACCACGTACTTCGAGACCGTGACCGCGATGGCATTCCTGA
TCTTCGCCGAAGAGGCCGTCGATATCGTGGTGCTCGAAGTCGGACTCGGCGGCCGGCTTGACGCCACCAATGTCGTCCTG
CCCGAACTCTGCGTGATCACGCCGGTGGATTTCGATCACGAAGCCTTCCTGGGCCGCAGCCTTCAGGAGATCGCCGGGGA
GAAGGCCGGCATCCTGAAAGCGGGAGTGCCCGCCGTCTTCGCCCGCCAGCGCGCGGAAGCCTCCGCCGTTCTCGATCAGC
GCGCGGCGCAGCTTTCGATTCCGATTGCCCGTACCGTCGCCTGGCCGGTGGATGATCTCGTACTCGACTCCCGCGGCAGC
CGTTTCCTGCTCAGCGGCGAGCGCGACCTCCACATTGCGTGTCCCCTGGCCGGCGAACACCAGGTGGAAAATGCGGCCAC
GGCCGCGATCGCCCTGACCCGGCTCGGCGTCTCCGACCACAGCATCGAGACCGGTATCGCCCAAACCCGCTGGCCGGGCC
GCCTCGAGCGCGTCTCCGAACATCCCGAGATCATCCTCGATGGCGCCCACAATCCCGCCGGAGCCCGCGCGCTCGCCGCC
TACATCGAGCGCTTCTACGCCGGCCGCCGCGTCCGCCTGATCTATGGCGCCATGCGCGATAAGGCCATCGACGAGATCGC
CGGCATTCTGTTCCCGCTCGCGCACCAGGTGATTCTCACCGCGCCGCGCCAGGCGCGGGCGCTGGCCCCCGAAGCCATCC
GCGAGATTGCCGACCATCCCGACGTCCAGGTGGCGCCCGATCTCGCGCAGGCAATCGCGCTGCTCTGCGATGCCGCATCC
AACGACGCCGTCTTCATCACCGGCTCGCTCTTTCTTGTCGCCGAGGCGCGAGCTCTACTGCTGGTATGA

Upstream 100 bases:

>100_bases
ATGACCGACGAGCCGCGACCGTGAGGGAACGCGACCCGCACGTCACCGGTGGTTGCGCTCCCTTTCGGTCGCGGCTCGTT
GCGGCCATGAGGGCGCCTTT

Downstream 100 bases:

>100_bases
TTGGTGCGAAACGATGAGTCTTGTCCGGTCCCTGCTTTTCTCTACTCCTCTTATTGTCCTTTGCACGATCGTGATGGGCA
CCCTCTCCCTGATCGCGTCC

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNYPDSVQFLYALGNEVKTAKLGLERIARVLEALGNPHRRTRIVHVAGTNGKGSTCAMIESGLRASGRSTGLFTSPHLAE
PTERIRINGVPVSAERFSAAFDRVHAAVEQLLAVNAIDLHTTYFETVTAMAFLIFAEEAVDIVVLEVGLGGRLDATNVVL
PELCVITPVDFDHEAFLGRSLQEIAGEKAGILKAGVPAVFARQRAEASAVLDQRAAQLSIPIARTVAWPVDDLVLDSRGS
RFLLSGERDLHIACPLAGEHQVENAATAAIALTRLGVSDHSIETGIAQTRWPGRLERVSEHPEIILDGAHNPAGARALAA
YIERFYAGRRVRLIYGAMRDKAIDEIAGILFPLAHQVILTAPRQARALAPEAIREIADHPDVQVAPDLAQAIALLCDAAS
NDAVFITGSLFLVAEARALLLV

Sequences:

>Translated_422_residues
MNYPDSVQFLYALGNEVKTAKLGLERIARVLEALGNPHRRTRIVHVAGTNGKGSTCAMIESGLRASGRSTGLFTSPHLAE
PTERIRINGVPVSAERFSAAFDRVHAAVEQLLAVNAIDLHTTYFETVTAMAFLIFAEEAVDIVVLEVGLGGRLDATNVVL
PELCVITPVDFDHEAFLGRSLQEIAGEKAGILKAGVPAVFARQRAEASAVLDQRAAQLSIPIARTVAWPVDDLVLDSRGS
RFLLSGERDLHIACPLAGEHQVENAATAAIALTRLGVSDHSIETGIAQTRWPGRLERVSEHPEIILDGAHNPAGARALAA
YIERFYAGRRVRLIYGAMRDKAIDEIAGILFPLAHQVILTAPRQARALAPEAIREIADHPDVQVAPDLAQAIALLCDAAS
NDAVFITGSLFLVAEARALLLV
>Mature_422_residues
MNYPDSVQFLYALGNEVKTAKLGLERIARVLEALGNPHRRTRIVHVAGTNGKGSTCAMIESGLRASGRSTGLFTSPHLAE
PTERIRINGVPVSAERFSAAFDRVHAAVEQLLAVNAIDLHTTYFETVTAMAFLIFAEEAVDIVVLEVGLGGRLDATNVVL
PELCVITPVDFDHEAFLGRSLQEIAGEKAGILKAGVPAVFARQRAEASAVLDQRAAQLSIPIARTVAWPVDDLVLDSRGS
RFLLSGERDLHIACPLAGEHQVENAATAAIALTRLGVSDHSIETGIAQTRWPGRLERVSEHPEIILDGAHNPAGARALAA
YIERFYAGRRVRLIYGAMRDKAIDEIAGILFPLAHQVILTAPRQARALAPEAIREIADHPDVQVAPDLAQAIALLCDAAS
NDAVFITGSLFLVAEARALLLV

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=420, Percent_Identity=32.1428571428571, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI66932990, Length=361, Percent_Identity=34.0720221606648, Blast_Score=176, Evalue=5e-44,
Organism=Escherichia coli, GI1788654, Length=405, Percent_Identity=34.8148148148148, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71984923, Length=436, Percent_Identity=30.7339449541284, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17553148, Length=436, Percent_Identity=30.7339449541284, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17553150, Length=436, Percent_Identity=30.7339449541284, Blast_Score=175, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6323760, Length=427, Percent_Identity=32.5526932084309, Blast_Score=200, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6324815, Length=333, Percent_Identity=28.8288288288288, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6322718, Length=295, Percent_Identity=26.4406779661017, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24641571, Length=311, Percent_Identity=32.475884244373, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24581568, Length=204, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 45296; Mature: 45296

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYPDSVQFLYALGNEVKTAKLGLERIARVLEALGNPHRRTRIVHVAGTNGKGSTCAMIE
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCCHHHHH
SGLRASGRSTGLFTSPHLAEPTERIRINGVPVSAERFSAAFDRVHAAVEQLLAVNAIDLH
HCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHH
TTYFETVTAMAFLIFAEEAVDIVVLEVGLGGRLDATNVVLPELCVITPVDFDHEAFLGRS
HHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCEECCHHHEEECCCCCHHHHHHHH
LQEIAGEKAGILKAGVPAVFARQRAEASAVLDQRAAQLSIPIARTVAWPVDDLVLDSRGS
HHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHCCCCCC
RFLLSGERDLHIACPLAGEHQVENAATAAIALTRLGVSDHSIETGIAQTRWPGRLERVSE
EEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCHHHHHHCC
HPEIILDGAHNPAGARALAAYIERFYAGRRVRLIYGAMRDKAIDEIAGILFPLAHQVILT
CCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHC
APRQARALAPEAIREIADHPDVQVAPDLAQAIALLCDAASNDAVFITGSLFLVAEARALL
CCHHHHHCCHHHHHHHHCCCCCEECHHHHHHHHHHHCCCCCCEEEEECCEEEEECCHHHH
LV
CC
>Mature Secondary Structure
MNYPDSVQFLYALGNEVKTAKLGLERIARVLEALGNPHRRTRIVHVAGTNGKGSTCAMIE
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCCHHHHH
SGLRASGRSTGLFTSPHLAEPTERIRINGVPVSAERFSAAFDRVHAAVEQLLAVNAIDLH
HCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHH
TTYFETVTAMAFLIFAEEAVDIVVLEVGLGGRLDATNVVLPELCVITPVDFDHEAFLGRS
HHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCEECCHHHEEECCCCCHHHHHHHH
LQEIAGEKAGILKAGVPAVFARQRAEASAVLDQRAAQLSIPIARTVAWPVDDLVLDSRGS
HHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHCCCCCC
RFLLSGERDLHIACPLAGEHQVENAATAAIALTRLGVSDHSIETGIAQTRWPGRLERVSE
EEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCHHHHHHCC
HPEIILDGAHNPAGARALAAYIERFYAGRRVRLIYGAMRDKAIDEIAGILFPLAHQVILT
CCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHC
APRQARALAPEAIREIADHPDVQVAPDLAQAIALLCDAASNDAVFITGSLFLVAEARALL
CCHHHHHCCHHHHHHHHCCCCCEECHHHHHHHHHHHCCCCCCEEEEECCEEEEECCHHHH
LV
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]