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The map label for this gene is ilvE [H]

Identifier: 116624649

GI number: 116624649

Start: 6985714

End: 6986652

Strand: Reverse

Name: ilvE [H]

Synonym: Acid_5573

Alternate gene names: 116624649

Gene position: 6986652-6985714 (Counterclockwise)

Preceding gene: 116624650

Following gene: 116624648

Centisome position: 70.11

GC content: 60.49

Gene sequence:

>939_bases
ATGTTGACGCCCACTGAAAAGATCTGGCATAACGGCCGCCTCATTGCATGGCAGGAGGCGACCATTCACGTACTGTCGCA
TGTAGTGAGTTACGGCAGCAGCGTATTCGAGGGCATTCGGTGTTATTCGACGCCGAGCGGACCGGCGGTATTCCGGCTGC
GCGAGCATGTGCGCCGGATGACGGATTCGGCCAAGATTTACCGGATGGACAACCTCGGTTTCAGCGCCGCGGAACTCGCG
GACGCGATGCTGGAAGTGGTGCGGGTGAACAAAATGGATGCCTGTTACATCCGGCCGATCGTGATGCGCGGATATGGCGA
TATCGGGGTGAACGGCATGAAGAACCCGATCGATGTGTACATCGCATGCTGGAGCTGGGGCAAGTATCTGGGCGAGGAAG
CGCTGGCGGAAGGCGTGGATGTTTGCGTTTCCAGCTGGAACCGGATGGCGCCGAACACGCTGCCTGCGCTGGCCAAGGCG
GGCGCGAATTACATGAATTCGCAGCTCATGAAGATGGAGGCGATCACCAACGGGTATGCGGAAGGGATCGCGCTGGATGT
GACGGGCTACGTGAGCGAAGGCTCGGGCGAGAACATCTTCCTGGTACGCGATGGCAAGATCCACACTCCGCCGCTGGGGG
CGAGCGTGTTGCCGGGAATCACGCGCGACGCCATCCTGACGCTGGCGCGGGACGCGGGAATCCCGGTTGTAGAGACGATC
ATTCCGCGGGAACTGCTGTACATCGCCGACGAAGTATTCTTCAGCGGGACGGCGGCTGAGATCACGCCGATCCGGTCGAT
CGACCGGATCACGATCGGCAACGGACGGCGCGGACCCATCGCGGAGCAGTTGCAGAAGGAATTCTTCGGAGTGATCAACG
GCACTAAGAAGGATACGTACGGCTGGCTCTCGCACGTACACGCCGCTGCTAACGCGTAA

Upstream 100 bases:

>100_bases
GCCTTGCTTTGGACGCGAAATCGGAAGCGCTACCCGATGCAGGATGTGAAGTTTTTCTGAGCCGGGACAGCGGCGGGACC
GCCCAAGTTATAATCGATCC

Downstream 100 bases:

>100_bases
GCGTTGGCAATGCTCCTGGGGCCGGAGACACCGGCCCTTAAAATACAGGAGGGAATGGAGGACGCCCCGGCTGAACTGCG
CGACCAGAATGACTACCTCC

Product: branched chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MLTPTEKIWHNGRLIAWQEATIHVLSHVVSYGSSVFEGIRCYSTPSGPAVFRLREHVRRMTDSAKIYRMDNLGFSAAELA
DAMLEVVRVNKMDACYIRPIVMRGYGDIGVNGMKNPIDVYIACWSWGKYLGEEALAEGVDVCVSSWNRMAPNTLPALAKA
GANYMNSQLMKMEAITNGYAEGIALDVTGYVSEGSGENIFLVRDGKIHTPPLGASVLPGITRDAILTLARDAGIPVVETI
IPRELLYIADEVFFSGTAAEITPIRSIDRITIGNGRRGPIAEQLQKEFFGVINGTKKDTYGWLSHVHAAANA

Sequences:

>Translated_312_residues
MLTPTEKIWHNGRLIAWQEATIHVLSHVVSYGSSVFEGIRCYSTPSGPAVFRLREHVRRMTDSAKIYRMDNLGFSAAELA
DAMLEVVRVNKMDACYIRPIVMRGYGDIGVNGMKNPIDVYIACWSWGKYLGEEALAEGVDVCVSSWNRMAPNTLPALAKA
GANYMNSQLMKMEAITNGYAEGIALDVTGYVSEGSGENIFLVRDGKIHTPPLGASVLPGITRDAILTLARDAGIPVVETI
IPRELLYIADEVFFSGTAAEITPIRSIDRITIGNGRRGPIAEQLQKEFFGVINGTKKDTYGWLSHVHAAANA
>Mature_312_residues
MLTPTEKIWHNGRLIAWQEATIHVLSHVVSYGSSVFEGIRCYSTPSGPAVFRLREHVRRMTDSAKIYRMDNLGFSAAELA
DAMLEVVRVNKMDACYIRPIVMRGYGDIGVNGMKNPIDVYIACWSWGKYLGEEALAEGVDVCVSSWNRMAPNTLPALAKA
GANYMNSQLMKMEAITNGYAEGIALDVTGYVSEGSGENIFLVRDGKIHTPPLGASVLPGITRDAILTLARDAGIPVVETI
IPRELLYIADEVFFSGTAAEITPIRSIDRITIGNGRRGPIAEQLQKEFFGVINGTKKDTYGWLSHVHAAANA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=301, Percent_Identity=54.485049833887, Blast_Score=344, Evalue=5e-96,
Organism=Saccharomyces cerevisiae, GI6322608, Length=328, Percent_Identity=26.8292682926829, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6322002, Length=272, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34138; Mature: 34138

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPTEKIWHNGRLIAWQEATIHVLSHVVSYGSSVFEGIRCYSTPSGPAVFRLREHVRRM
CCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHCHHHHHCCEEECCCCCHHHHHHHHHHHHH
TDSAKIYRMDNLGFSAAELADAMLEVVRVNKMDACYIRPIVMRGYGDIGVNGMKNPIDVY
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCCCCCEEEE
IACWSWGKYLGEEALAEGVDVCVSSWNRMAPNTLPALAKAGANYMNSQLMKMEAITNGYA
EEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHCHHH
EGIALDVTGYVSEGSGENIFLVRDGKIHTPPLGASVLPGITRDAILTLARDAGIPVVETI
CCEEEEEEEEEECCCCCEEEEEECCEECCCCCCHHHCCCCHHHHHHHHHHCCCCCHHHHH
IPRELLYIADEVFFSGTAAEITPIRSIDRITIGNGRRGPIAEQLQKEFFGVINGTKKDTY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHH
GWLSHVHAAANA
HHHHHHHHHCCC
>Mature Secondary Structure
MLTPTEKIWHNGRLIAWQEATIHVLSHVVSYGSSVFEGIRCYSTPSGPAVFRLREHVRRM
CCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHCHHHHHCCEEECCCCCHHHHHHHHHHHHH
TDSAKIYRMDNLGFSAAELADAMLEVVRVNKMDACYIRPIVMRGYGDIGVNGMKNPIDVY
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCCCCCEEEE
IACWSWGKYLGEEALAEGVDVCVSSWNRMAPNTLPALAKAGANYMNSQLMKMEAITNGYA
EEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHCHHH
EGIALDVTGYVSEGSGENIFLVRDGKIHTPPLGASVLPGITRDAILTLARDAGIPVVETI
CCEEEEEEEEEECCCCCEEEEEECCEECCCCCCHHHCCCCHHHHHHHHHHCCCCCHHHHH
IPRELLYIADEVFFSGTAAEITPIRSIDRITIGNGRRGPIAEQLQKEFFGVINGTKKDTY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHH
GWLSHVHAAANA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]