Click here to switch to the map view.

The map label for this gene is nolF [H]

Identifier: 116624595

GI number: 116624595

Start: 6920551

End: 6921774

Strand: Direct

Name: nolF [H]

Synonym: Acid_5519

Alternate gene names: 116624595

Gene position: 6920551-6921774 (Clockwise)

Preceding gene: 116624594

Following gene: 116624596

Centisome position: 69.44

GC content: 66.58

Gene sequence:

>1224_bases
ATGCTCCTTCGCCCTGGGAATGCCCTCATCAGAGCCGCGACCGTTACGGAGCAATCGCTTCTTACGGCCTGCCGCACTGT
GGGGCAGGCAATCCTGCCTGCAGCCGGCTTTCAGCCGGCTTGCCCCGCGCCAACGCGGCGTGTCCCTCTTCATAGGTTCG
CAAGCATCGCGCTGCCTCTCCTGCTCGCTTGCGCCCTCTTCGCGCAGCAGCCCCCCGAACTCGTGGCCGTGGTCGCCAAG
CCCCTCGATCGCAAGCTCCCCCTCCCGGGCGAGATCTCCCCCTATCAGGACGTCGAGATCCACGCCAAGATCGCGGGCTT
CGTCGAGCGCGTCCTGGTCGATCGCGGCAGTGCCGTGAAGAAGGGCGAACCGCTCGCCACCCTCGACGCCCCCGAGCTGA
AGACCCAGCGCGCCGAAGCCCAGTCCAAGGTGCAGGCGATGCAAGCCCAACGCGCCGAGGCCGAGGCCCGCCTGGTCGGC
GAGCAGGGTACTTACGATCACCTCAAGGCCGCCTCCGCCACTCCCGGTGTCATCGCCGGTATCGACCTCATCGTCGCTGA
AAAACGCGTGGACGCCGCCCGCGCCCAGGTTACCGCCGTCGAAGCCGGCATCAACGCCGCGCAGTCCGCCGTCAAAGTCA
TCGAGGACATGCAGCAGTATCTCGAACTCACCGCGCCCTTCGACGGAGTCATCACGGAGCGCGGCGTCTCCCCCGGTGCG
CTCGTGGGACCGAACAGCAAGAGCCTCTTTCGCCTGGAACAAAACTCCCGCCTGCGCCTCGTGGTCGCGGTGCCCGAGCC
CGACGTCTCCGGCATCGCCGCCGGCGCCCACGTCACCTTCACCGTCCCTGCCTACCCCGGCCAGCAGTTTACCGGCACCA
TCGCGCGCGTAGCGCATTCCGTGGACGTGAAGACGCGCTCCATGCCGGTGGAACTCGACGTCGATAACGCACACCGCCAG
CTGTCTCCCGGAATGTATCCCACCGTGAACTGGCCCGTGAAGCGCTCCCGCCCCACGCTCATCGTGCCCGGCTCCGCCGT
CGCGGGCAACACCGAGCGCACCTTCGTCATCCGCGCTAACAACGGCGTCGCCGAGTGGGTCAACGTCTCAAAAGGCGTCA
CCGTCACCCCAGATACCATCGAAGTCTTCGGCCCCCTGCAACCCGGCGACATGATCGTCAAACGTGCCACCGACGAAATC
CGCGAAGGAACGAAACTGAAGTGA

Upstream 100 bases:

>100_bases
TCGCTCTACACAATCCTTCAGCGCCGCGCTGGAACCCAATCGCCCTCTCTCGACCCCGATGATTCCGCCAGCAGGTATTA
CGACCATGCGTAATTCGTTA

Downstream 100 bases:

>100_bases
CCCGGCCTGAAATGTACCTTCGGCTCCGCGAGCACAACGGCCCGTGGGACATGATCGTCATCGGAGGCGGCGCTACCGGC
GCCGGCGTCGCCGTGGACGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MLLRPGNALIRAATVTEQSLLTACRTVGQAILPAAGFQPACPAPTRRVPLHRFASIALPLLLACALFAQQPPELVAVVAK
PLDRKLPLPGEISPYQDVEIHAKIAGFVERVLVDRGSAVKKGEPLATLDAPELKTQRAEAQSKVQAMQAQRAEAEARLVG
EQGTYDHLKAASATPGVIAGIDLIVAEKRVDAARAQVTAVEAGINAAQSAVKVIEDMQQYLELTAPFDGVITERGVSPGA
LVGPNSKSLFRLEQNSRLRLVVAVPEPDVSGIAAGAHVTFTVPAYPGQQFTGTIARVAHSVDVKTRSMPVELDVDNAHRQ
LSPGMYPTVNWPVKRSRPTLIVPGSAVAGNTERTFVIRANNGVAEWVNVSKGVTVTPDTIEVFGPLQPGDMIVKRATDEI
REGTKLK

Sequences:

>Translated_407_residues
MLLRPGNALIRAATVTEQSLLTACRTVGQAILPAAGFQPACPAPTRRVPLHRFASIALPLLLACALFAQQPPELVAVVAK
PLDRKLPLPGEISPYQDVEIHAKIAGFVERVLVDRGSAVKKGEPLATLDAPELKTQRAEAQSKVQAMQAQRAEAEARLVG
EQGTYDHLKAASATPGVIAGIDLIVAEKRVDAARAQVTAVEAGINAAQSAVKVIEDMQQYLELTAPFDGVITERGVSPGA
LVGPNSKSLFRLEQNSRLRLVVAVPEPDVSGIAAGAHVTFTVPAYPGQQFTGTIARVAHSVDVKTRSMPVELDVDNAHRQ
LSPGMYPTVNWPVKRSRPTLIVPGSAVAGNTERTFVIRANNGVAEWVNVSKGVTVTPDTIEVFGPLQPGDMIVKRATDEI
REGTKLK
>Mature_407_residues
MLLRPGNALIRAATVTEQSLLTACRTVGQAILPAAGFQPACPAPTRRVPLHRFASIALPLLLACALFAQQPPELVAVVAK
PLDRKLPLPGEISPYQDVEIHAKIAGFVERVLVDRGSAVKKGEPLATLDAPELKTQRAEAQSKVQAMQAQRAEAEARLVG
EQGTYDHLKAASATPGVIAGIDLIVAEKRVDAARAQVTAVEAGINAAQSAVKVIEDMQQYLELTAPFDGVITERGVSPGA
LVGPNSKSLFRLEQNSRLRLVVAVPEPDVSGIAAGAHVTFTVPAYPGQQFTGTIARVAHSVDVKTRSMPVELDVDNAHRQ
LSPGMYPTVNWPVKRSRPTLIVPGSAVAGNTERTFVIRANNGVAEWVNVSKGVTVTPDTIEVFGPLQPGDMIVKRATDEI
REGTKLK

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=346, Percent_Identity=21.9653179190751, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789929, Length=388, Percent_Identity=23.7113402061856, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87081796, Length=312, Percent_Identity=24.0384615384615, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43318; Mature: 43318

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRPGNALIRAATVTEQSLLTACRTVGQAILPAAGFQPACPAPTRRVPLHRFASIALPL
CEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LLACALFAQQPPELVAVVAKPLDRKLPLPGEISPYQDVEIHAKIAGFVERVLVDRGSAVK
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCC
KGEPLATLDAPELKTQRAEAQSKVQAMQAQRAEAEARLVGEQGTYDHLKAASATPGVIAG
CCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHCCCCCCHHCC
IDLIVAEKRVDAARAQVTAVEAGINAAQSAVKVIEDMQQYLELTAPFDGVITERGVSPGA
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
LVGPNSKSLFRLEQNSRLRLVVAVPEPDVSGIAAGAHVTFTVPAYPGQQFTGTIARVAHS
EECCCCCCEEEEECCCCEEEEEEECCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHHHH
VDVKTRSMPVELDVDNAHRQLSPGMYPTVNWPVKRSRPTLIVPGSAVAGNTERTFVIRAN
CCCEECCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCCCEEECCCCEECCCCEEEEEEEC
NGVAEWVNVSKGVTVTPDTIEVFGPLQPGDMIVKRATDEIREGTKLK
CCHHHHHHCCCCCEECCCCEEEECCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLLRPGNALIRAATVTEQSLLTACRTVGQAILPAAGFQPACPAPTRRVPLHRFASIALPL
CEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LLACALFAQQPPELVAVVAKPLDRKLPLPGEISPYQDVEIHAKIAGFVERVLVDRGSAVK
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCC
KGEPLATLDAPELKTQRAEAQSKVQAMQAQRAEAEARLVGEQGTYDHLKAASATPGVIAG
CCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHCCCCCCHHCC
IDLIVAEKRVDAARAQVTAVEAGINAAQSAVKVIEDMQQYLELTAPFDGVITERGVSPGA
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
LVGPNSKSLFRLEQNSRLRLVVAVPEPDVSGIAAGAHVTFTVPAYPGQQFTGTIARVAHS
EECCCCCCEEEEECCCCEEEEEEECCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHHHH
VDVKTRSMPVELDVDNAHRQLSPGMYPTVNWPVKRSRPTLIVPGSAVAGNTERTFVIRAN
CCCEECCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCCCEEECCCCEECCCCEEEEEEEC
NGVAEWVNVSKGVTVTPDTIEVFGPLQPGDMIVKRATDEIREGTKLK
CCHHHHHHCCCCCEECCCCEEEECCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]