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The map label for this gene is yjfH [C]

Identifier: 116624472

GI number: 116624472

Start: 6758015

End: 6758845

Strand: Reverse

Name: yjfH [C]

Synonym: Acid_5394

Alternate gene names: 116624472

Gene position: 6758845-6758015 (Counterclockwise)

Preceding gene: 116624475

Following gene: 116624471

Centisome position: 67.82

GC content: 67.51

Gene sequence:

>831_bases
TTGTGCGTTATTCTGATCGTTCAGAATCATTGTAAGCAGATGGCGAAGCCGGAAACGATTACGAGCGCGGCCAACCCGCT
GTTGAAGGACGTGCGCCGCGCCATTTCCCACGGCGCCCTTACCGAACAAGGCTGGTGCGTGGCCGAGACTCCCCACCTGC
TCGAAGAGGCGCTCCACAGCCAGTGCGAGGTCAAGACCGTGCTCGCTTCCGAAAGCGCGCTCGACGGTGCCTCGGCGCAT
TTGAGGAGCCTGCCGCTGAAGCTGGTCTCCCTGCCGGACGCGCTGTTCGCCGGCATCGCCGGCACCGAGACCACCCAGGG
AGTGATCGCGCTGGTGCAGCCGCCCGCGTGGAAACTGGAGCAGCTCTTCCAGCGGCCGGCGCTCGTGGTGGTGCTGGACG
GTCTGCAGGACCCGGGCAACGCCGGGACCATTGTGCGCGCGGCCGAAGCCTTCGGCGCTACCGGCGTCGTCTTCCTGAAG
GGCGCAGTGAGCCCGTACAATCCGAAGACGCTGCGCGCCTCGGCAGGCTCGCTCTTCCGCGTCCCCTTCCTGCACGGCGT
GGATGCCGCGGTGGCCTGCACGGCACTCGCGCAGCACCAGGTCGAGCTCTTCGCCGGCGTTCCGGCCGGGGCGTCGTCGC
GCACGCTGGCCGCCGTCGATCTCGCTCGTCCCTGCGGCCTGGTCATTGGCAATGAGGCGCATGGCGTCAGCCGCGGTCTG
CGCGCCGCTGCCCATGACGTGAGCATTCCGACAGTGGGCGTGGAATCGCTGAATGCGGCGATGGCGGCCTCCATTCTGCT
CTATGAAGCGCGCCGCCAGAGGATGCTGTGA

Upstream 100 bases:

>100_bases
TCGCGGACGCTCTGCTGTTCGATCCTCAGCGAAAGGAACGCGACGTAGAACATGACCACCACAGCCGCCAGCGCGATCCC
GTTCCATAGGAGCCTTTTCA

Downstream 100 bases:

>100_bases
GCCTGTTCGATACCACACCCCCGGATGTCCGCGACGAGAAGCGCCCGCTGGCCGATCGCATGCGACCCACCAGCCTGGAC
GAGTACATCGGCCAGGACCA

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MCVILIVQNHCKQMAKPETITSAANPLLKDVRRAISHGALTEQGWCVAETPHLLEEALHSQCEVKTVLASESALDGASAH
LRSLPLKLVSLPDALFAGIAGTETTQGVIALVQPPAWKLEQLFQRPALVVVLDGLQDPGNAGTIVRAAEAFGATGVVFLK
GAVSPYNPKTLRASAGSLFRVPFLHGVDAAVACTALAQHQVELFAGVPAGASSRTLAAVDLARPCGLVIGNEAHGVSRGL
RAAAHDVSIPTVGVESLNAAMAASILLYEARRQRML

Sequences:

>Translated_276_residues
MCVILIVQNHCKQMAKPETITSAANPLLKDVRRAISHGALTEQGWCVAETPHLLEEALHSQCEVKTVLASESALDGASAH
LRSLPLKLVSLPDALFAGIAGTETTQGVIALVQPPAWKLEQLFQRPALVVVLDGLQDPGNAGTIVRAAEAFGATGVVFLK
GAVSPYNPKTLRASAGSLFRVPFLHGVDAAVACTALAQHQVELFAGVPAGASSRTLAAVDLARPCGLVIGNEAHGVSRGL
RAAAHDVSIPTVGVESLNAAMAASILLYEARRQRML
>Mature_276_residues
MCVILIVQNHCKQMAKPETITSAANPLLKDVRRAISHGALTEQGWCVAETPHLLEEALHSQCEVKTVLASESALDGASAH
LRSLPLKLVSLPDALFAGIAGTETTQGVIALVQPPAWKLEQLFQRPALVVVLDGLQDPGNAGTIVRAAEAFGATGVVFLK
GAVSPYNPKTLRASAGSLFRVPFLHGVDAAVACTALAQHQVELFAGVPAGASSRTLAAVDLARPCGLVIGNEAHGVSRGL
RAAAHDVSIPTVGVESLNAAMAASILLYEARRQRML

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=255, Percent_Identity=32.5490196078431, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1790623, Length=179, Percent_Identity=30.7262569832402, Blast_Score=68, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24666840, Length=201, Percent_Identity=29.3532338308458, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28806; Mature: 28806

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVILIVQNHCKQMAKPETITSAANPLLKDVRRAISHGALTEQGWCVAETPHLLEEALHS
CEEEEEECHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHH
QCEVKTVLASESALDGASAHLRSLPLKLVSLPDALFAGIAGTETTQGVIALVQPPAWKLE
HHHHHHHHHCCCCCCCHHHHHHHCCHHHHCCCHHHHHCCCCCCCCCCEEEEECCCCHHHH
QLFQRPALVVVLDGLQDPGNAGTIVRAAEAFGATGVVFLKGAVSPYNPKTLRASAGSLFR
HHHHCCEEEEEEECCCCCCCCCHHEEEHHHCCCCCEEEEECCCCCCCCCEEECCCCCEEE
VPFLHGVDAAVACTALAQHQVELFAGVPAGASSRTLAAVDLARPCGLVIGNEAHGVSRGL
CHHHHCCHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEHHCCCCCEEECCCCHHHHHHH
RAAAHDVSIPTVGVESLNAAMAASILLYEARRQRML
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCVILIVQNHCKQMAKPETITSAANPLLKDVRRAISHGALTEQGWCVAETPHLLEEALHS
CEEEEEECHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHH
QCEVKTVLASESALDGASAHLRSLPLKLVSLPDALFAGIAGTETTQGVIALVQPPAWKLE
HHHHHHHHHCCCCCCCHHHHHHHCCHHHHCCCHHHHHCCCCCCCCCCEEEEECCCCHHHH
QLFQRPALVVVLDGLQDPGNAGTIVRAAEAFGATGVVFLKGAVSPYNPKTLRASAGSLFR
HHHHCCEEEEEEECCCCCCCCCHHEEEHHHCCCCCEEEEECCCCCCCCCEEECCCCCEEE
VPFLHGVDAAVACTALAQHQVELFAGVPAGASSRTLAAVDLARPCGLVIGNEAHGVSRGL
CHHHHCCHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEHHCCCCCEEECCCCHHHHHHH
RAAAHDVSIPTVGVESLNAAMAASILLYEARRQRML
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]