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The map label for this gene is nusA [H]

Identifier: 116624262

GI number: 116624262

Start: 6505721

End: 6507349

Strand: Direct

Name: nusA [H]

Synonym: Acid_5180

Alternate gene names: 116624262

Gene position: 6505721-6507349 (Clockwise)

Preceding gene: 116624261

Following gene: 116624263

Centisome position: 65.28

GC content: 59.24

Gene sequence:

>1629_bases
TTGAGTGGTAGCCCGCCGGGGAAGATTTTAGCCAACACACATTTTGAATTGGATAACGAGGGCACTTTGGAAACGATTTT
TCAATCCATCGAAATCTTGAGCAAGGAAAAAGGCATCGACCCGCAGATCGTGCTCGACGCGGTCAAGGACGCGATGCTGA
TTGCCGCCCGGAAACACTATCGGAGCACGGAAGATTACGTGGCCGATATGGATCCGAAGACCGGGGCAATCAAGCTGTAC
GCGGTCAAGAAAGTGGTCGAGGCCGTGGAGGATCCTTCGCACGAGATGACCCTGGCGGAAGCCAGGCGGATCGATTCCAC
CGCCGAAGTCGGCGGCGAGGTCCGGATTCCCAAGAACACAGACGCCCTGGGTCGCATCTCGGCGCAGACCGCAAAACAGG
TCATCTTCCAGAAGGTCCGCGAGGCCGAACGCGAAACAGTTTTCAACGAGTACTCCGGTCGCACCGGGGAACTCGTCAAC
TGCACCATCAAGCGAGTCGAGGGACCCGATTACATTCTGGATCTGGGGAAGACCGAAGCCAAGCTGCCCAAAAAGGAACA
GTCGCGCCTGGAAGGCTATAGCGTGGGCGACCGCGTGCGCTGCATCATCAAGCTGGTGGATAAATCGAGCAAGGGACCGG
GCGTGCTGGTTTCGCGCGCGGCTCCGGAACTGGTCATGCGCCTGTTCGAACAGGAAGTACCCGAAATTTACGATGGCACC
GTCGCCATTCGCGGCTGCGCGCGCGAAGCCGGGGAGCGCACCAAGATCGCGGTGCAGAGCCGCGACCGCGACGTGGATAG
CGTGGGCGCCTGCGTCGGCATGAAGGGCATGCGCGTGCAGTCCATCATCCGCGAACTGCGCGGCGAGAAGATCGACATCA
TCGAGTACTCGGAGGATTCGGTGGTCTTCGCCACCCACGCCCTGAGCCCGGCCAAGGTCAGTCGCATCACCGTCATCGAT
CCGCTCGAAAAGCACATGGAAGTGATCGTGGACGATTCGCAGCTCTCGCTTGCCATCGGCAAGAAGGGCCAGAACGTCCG
ATTGGCGGCGAAATTGCTCGGATGGAAGATCGATATCAAGAGCGAGGAAGAAAAGCGCCAGGAGGTGGAGTCGCAGATGG
CCGCCCTGGTTGCGCCCGGCGCTCCTGTATCGGTGCTGATCGATTACGGCATGGCGGAAGAGATGGCCGTGAAGCTACTC
GAAAACGGCGTCGCCACAATCGAAAAGCTGGGCGCGATGACTCCGGAAGAGCTCGAAGTGCTGCCGGGCATCGGACCCGA
ATCGGTGGAAGGAATTCAAACGGCTGTGGTGGCATATTACAGTCAGTTCGAAGATCCGGCTTCGGAGAATGCGGTATCGG
AAACCGCGGGATCCGAAAATACGGAACAGGCGACGGCCGAGACTACTTTCGAAACCGCGTCTGAACCCGCGCCGGCGGAT
GACGACCTGTACAATCCGGAGAGCGGGGAGGAACGCTTCGAGCCCGGCTCAGAAGCGGCTGAAGCAGTGAGCGAGGAAGA
ATCGGAGAGCACGGAACAGTTTGGTACGATAGTTGACGCGGGTTCTCCTCACAAACAAACCGACATGGCGGTTCCGGAGA
GCACCCCGGAAAAAGGTAGCGGCGAGTAA

Upstream 100 bases:

>100_bases
ACGCTCGCGGGATTCGCGGACGGTGTGATCTCCCTGGAAGTCGCGCCCGGCGAAATCCGCCGGCTTCCGTTCGATCAAGT
CAAGAAGGCCAACCTGAAGT

Downstream 100 bases:

>100_bases
GTGCGCCGTCCTCAAGTAGTAGCAGATTGCTTTCTCTATGAAGAAGATTCGGATCAACGAGCTGGCGCGGGAATTGGAAG
TCAAAGCTCACGAAATACTG

Product: NusA antitermination factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MSGSPPGKILANTHFELDNEGTLETIFQSIEILSKEKGIDPQIVLDAVKDAMLIAARKHYRSTEDYVADMDPKTGAIKLY
AVKKVVEAVEDPSHEMTLAEARRIDSTAEVGGEVRIPKNTDALGRISAQTAKQVIFQKVREAERETVFNEYSGRTGELVN
CTIKRVEGPDYILDLGKTEAKLPKKEQSRLEGYSVGDRVRCIIKLVDKSSKGPGVLVSRAAPELVMRLFEQEVPEIYDGT
VAIRGCAREAGERTKIAVQSRDRDVDSVGACVGMKGMRVQSIIRELRGEKIDIIEYSEDSVVFATHALSPAKVSRITVID
PLEKHMEVIVDDSQLSLAIGKKGQNVRLAAKLLGWKIDIKSEEEKRQEVESQMAALVAPGAPVSVLIDYGMAEEMAVKLL
ENGVATIEKLGAMTPEELEVLPGIGPESVEGIQTAVVAYYSQFEDPASENAVSETAGSENTEQATAETTFETASEPAPAD
DDLYNPESGEERFEPGSEAAEAVSEEESESTEQFGTIVDAGSPHKQTDMAVPESTPEKGSGE

Sequences:

>Translated_542_residues
MSGSPPGKILANTHFELDNEGTLETIFQSIEILSKEKGIDPQIVLDAVKDAMLIAARKHYRSTEDYVADMDPKTGAIKLY
AVKKVVEAVEDPSHEMTLAEARRIDSTAEVGGEVRIPKNTDALGRISAQTAKQVIFQKVREAERETVFNEYSGRTGELVN
CTIKRVEGPDYILDLGKTEAKLPKKEQSRLEGYSVGDRVRCIIKLVDKSSKGPGVLVSRAAPELVMRLFEQEVPEIYDGT
VAIRGCAREAGERTKIAVQSRDRDVDSVGACVGMKGMRVQSIIRELRGEKIDIIEYSEDSVVFATHALSPAKVSRITVID
PLEKHMEVIVDDSQLSLAIGKKGQNVRLAAKLLGWKIDIKSEEEKRQEVESQMAALVAPGAPVSVLIDYGMAEEMAVKLL
ENGVATIEKLGAMTPEELEVLPGIGPESVEGIQTAVVAYYSQFEDPASENAVSETAGSENTEQATAETTFETASEPAPAD
DDLYNPESGEERFEPGSEAAEAVSEEESESTEQFGTIVDAGSPHKQTDMAVPESTPEKGSGE
>Mature_541_residues
SGSPPGKILANTHFELDNEGTLETIFQSIEILSKEKGIDPQIVLDAVKDAMLIAARKHYRSTEDYVADMDPKTGAIKLYA
VKKVVEAVEDPSHEMTLAEARRIDSTAEVGGEVRIPKNTDALGRISAQTAKQVIFQKVREAERETVFNEYSGRTGELVNC
TIKRVEGPDYILDLGKTEAKLPKKEQSRLEGYSVGDRVRCIIKLVDKSSKGPGVLVSRAAPELVMRLFEQEVPEIYDGTV
AIRGCAREAGERTKIAVQSRDRDVDSVGACVGMKGMRVQSIIRELRGEKIDIIEYSEDSVVFATHALSPAKVSRITVIDP
LEKHMEVIVDDSQLSLAIGKKGQNVRLAAKLLGWKIDIKSEEEKRQEVESQMAALVAPGAPVSVLIDYGMAEEMAVKLLE
NGVATIEKLGAMTPEELEVLPGIGPESVEGIQTAVVAYYSQFEDPASENAVSETAGSENTEQATAETTFETASEPAPADD
DLYNPESGEERFEPGSEAAEAVSEEESESTEQFGTIVDAGSPHKQTDMAVPESTPEKGSGE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=410, Percent_Identity=34.390243902439, Blast_Score=239, Evalue=3e-64,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 58860; Mature: 58729

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS50084 KH_TYPE_1 ; PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSPPGKILANTHFELDNEGTLETIFQSIEILSKEKGIDPQIVLDAVKDAMLIAARKHY
CCCCCCCCEEEECEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
RSTEDYVADMDPKTGAIKLYAVKKVVEAVEDPSHEMTLAEARRIDSTAEVGGEVRIPKNT
CCCHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHCCCEEECCCCC
DALGRISAQTAKQVIFQKVREAERETVFNEYSGRTGELVNCTIKRVEGPDYILDLGKTEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEECCCCHH
KLPKKEQSRLEGYSVGDRVRCIIKLVDKSSKGPGVLVSRAAPELVMRLFEQEVPEIYDGT
CCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCHHHHCCC
VAIRGCAREAGERTKIAVQSRDRDVDSVGACVGMKGMRVQSIIRELRGEKIDIIEYSEDS
EEEEHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCC
VVFATHALSPAKVSRITVIDPLEKHMEVIVDDSQLSLAIGKKGQNVRLAAKLLGWKIDIK
EEEEEECCCCHHHCEEEEECHHHHHHHEEECCCCEEEEECCCCCCEEEEHHHHCEEEECC
SEEEKRQEVESQMAALVAPGAPVSVLIDYGMAEEMAVKLLENGVATIEKLGAMTPEELEV
CHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LPGIGPESVEGIQTAVVAYYSQFEDPASENAVSETAGSENTEQATAETTFETASEPAPAD
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCC
DDLYNPESGEERFEPGSEAAEAVSEEESESTEQFGTIVDAGSPHKQTDMAVPESTPEKGS
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCC
GE
CC
>Mature Secondary Structure 
SGSPPGKILANTHFELDNEGTLETIFQSIEILSKEKGIDPQIVLDAVKDAMLIAARKHY
CCCCCCCEEEECEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
RSTEDYVADMDPKTGAIKLYAVKKVVEAVEDPSHEMTLAEARRIDSTAEVGGEVRIPKNT
CCCHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHCCCEEECCCCC
DALGRISAQTAKQVIFQKVREAERETVFNEYSGRTGELVNCTIKRVEGPDYILDLGKTEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEECCCCHH
KLPKKEQSRLEGYSVGDRVRCIIKLVDKSSKGPGVLVSRAAPELVMRLFEQEVPEIYDGT
CCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCHHHHCCC
VAIRGCAREAGERTKIAVQSRDRDVDSVGACVGMKGMRVQSIIRELRGEKIDIIEYSEDS
EEEEHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCC
VVFATHALSPAKVSRITVIDPLEKHMEVIVDDSQLSLAIGKKGQNVRLAAKLLGWKIDIK
EEEEEECCCCHHHCEEEEECHHHHHHHEEECCCCEEEEECCCCCCEEEEHHHHCEEEECC
SEEEKRQEVESQMAALVAPGAPVSVLIDYGMAEEMAVKLLENGVATIEKLGAMTPEELEV
CHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LPGIGPESVEGIQTAVVAYYSQFEDPASENAVSETAGSENTEQATAETTFETASEPAPAD
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCC
DDLYNPESGEERFEPGSEAAEAVSEEESESTEQFGTIVDAGSPHKQTDMAVPESTPEKGS
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCC
GE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]