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The map label for this gene is hoxH [H]

Identifier: 116624100

GI number: 116624100

Start: 6330919

End: 6332343

Strand: Reverse

Name: hoxH [H]

Synonym: Acid_5015

Alternate gene names: 116624100

Gene position: 6332343-6330919 (Counterclockwise)

Preceding gene: 116624101

Following gene: 116624099

Centisome position: 63.54

GC content: 61.19

Gene sequence:

>1425_bases
ATGCCCCGGCAAAAGATCGTTATCGACCCGGTCACTCGCATCGAAGGCCACTCCAAAATCACTATCCACCTGGATGAGGA
GGGGCACGTGGAAGAGGCGTACTTCCACGTTACGCAATTCCGCGGTTTCGAAAAATTCTGCGAGGGCCGGCCCTACTACG
AGCTGCCGTCGCTCATGGCGCGCATCTGCGGAATCTGCCCCGTGAGCCACCTGATCGCCTCTGCCAAGGCCTGCGACGCA
ATTCTGGCGGTGCGCATTCCCGATGCGGCGATCCGCCTTCGCCGTGTGCTGAACCTGGCGCAGATTCTGCAATCGCACGC
GCTGAGCTTCTTCTACCTCTCGAGCCCGGACCTGCTGCTCGGCATGGATGCCGACCCGGCAGATCGCAACATCTTCGGCG
TGCTGCGCGCCAACCCGGCAGTGGCGAAGGATGGAATCCGTCTCCGCCAGTTCGGACAACAGGTCATCGAATGGCTCGCC
GGCAAGCGGATTCATCCGGCGTGGGTGGTGCCCGGCGGAGTCAGCAATCCGCTGACTGCGGAGAATCGCGACCGCATTCT
GGCGGGGATCCCCGAAGTCATGGCGATTGCCACGCGCACGCTCGATTGGTTCAAGAGCGAGCTCGAAGGCTTCCGTGAGG
AGATCCGCAGCTTCGCCAATTTCCCTACGCTGTTCATGGGACTGGTAGGGGAAGATGGCGGCCTGGAACACTACAACGGC
AAATTGCGTTTGGTGGATGCCGGCGGCCACATCGTCGCGGATCTTCCCGCCACCCGCTTCCCCGAATATCTGGCTGAGAT
TGCGCACCCATGGACCTACCTGAAGACCAGCTTCTACAAACCGCTCGGCTATCCCGACGGCGTATACCGAGTGGGTCCGC
TCGCGCGCCTCAACGTCATCGATGGCATCGGCACTCCGCGCGCCAGCCAGGAATGGGCCGAGTTTCGTGAACTCGATCGG
GGCCCCGTGCTGAGCAGTTTTTACTTCCACTACGCGCGTCTTATCGAACTGCTCTACGCCGTGGAAAAAATCGAAATGCT
GCTGACTCACGCGGACGCACTCGACTCCAATGTGCGCGCCCACGCCAGTGTCAACAACTCCGAGGGCATCGGCGTTTCGG
AGGCGCCGCGCGGCACCCTGATGCATCACTACAAGGTGGATCGCGACGGCCTGATCACCTGGGCCAACCTGATCATCGCC
ACCGGCCACAACAATGCCGCCATGAATCGGGGAGTGCTTCAGGTTGCCCGCCATTTCGTGCATGGCAACAAGCTCACCCA
AGGAATGTTAAATCGCGTGGAGGCCGTGATCCGCACCTTCGATCCGTGCCTGAGCTGCTCTACTCACTCCGATGGCCGCC
TGGCGCTTGAAGTGGAACTTCGCTCGCACGAAGGGGAGATGTTGGATCGATGCCGGACACTCTGA

Upstream 100 bases:

>100_bases
CGTGCCCGGATGCCCGCCGTCTGCCGACCTGATCTTCTCGGTCCTCAGCGAGTTGCTCGAAGGCAGAATGCCTAACCCCA
CCGCAAAATTCGGATAAATT

Downstream 100 bases:

>100_bases
TTATCGGGTACGGCAATGCGCTCCGCGCCGACGATGGCGCGGGCTACATCGCGGCGGAGCTGCTGCGTGAACGCATCCAC
AATCCCGCCGTTGAAGTCCT

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MPRQKIVIDPVTRIEGHSKITIHLDEEGHVEEAYFHVTQFRGFEKFCEGRPYYELPSLMARICGICPVSHLIASAKACDA
ILAVRIPDAAIRLRRVLNLAQILQSHALSFFYLSSPDLLLGMDADPADRNIFGVLRANPAVAKDGIRLRQFGQQVIEWLA
GKRIHPAWVVPGGVSNPLTAENRDRILAGIPEVMAIATRTLDWFKSELEGFREEIRSFANFPTLFMGLVGEDGGLEHYNG
KLRLVDAGGHIVADLPATRFPEYLAEIAHPWTYLKTSFYKPLGYPDGVYRVGPLARLNVIDGIGTPRASQEWAEFRELDR
GPVLSSFYFHYARLIELLYAVEKIEMLLTHADALDSNVRAHASVNNSEGIGVSEAPRGTLMHHYKVDRDGLITWANLIIA
TGHNNAAMNRGVLQVARHFVHGNKLTQGMLNRVEAVIRTFDPCLSCSTHSDGRLALEVELRSHEGEMLDRCRTL

Sequences:

>Translated_474_residues
MPRQKIVIDPVTRIEGHSKITIHLDEEGHVEEAYFHVTQFRGFEKFCEGRPYYELPSLMARICGICPVSHLIASAKACDA
ILAVRIPDAAIRLRRVLNLAQILQSHALSFFYLSSPDLLLGMDADPADRNIFGVLRANPAVAKDGIRLRQFGQQVIEWLA
GKRIHPAWVVPGGVSNPLTAENRDRILAGIPEVMAIATRTLDWFKSELEGFREEIRSFANFPTLFMGLVGEDGGLEHYNG
KLRLVDAGGHIVADLPATRFPEYLAEIAHPWTYLKTSFYKPLGYPDGVYRVGPLARLNVIDGIGTPRASQEWAEFRELDR
GPVLSSFYFHYARLIELLYAVEKIEMLLTHADALDSNVRAHASVNNSEGIGVSEAPRGTLMHHYKVDRDGLITWANLIIA
TGHNNAAMNRGVLQVARHFVHGNKLTQGMLNRVEAVIRTFDPCLSCSTHSDGRLALEVELRSHEGEMLDRCRTL
>Mature_473_residues
PRQKIVIDPVTRIEGHSKITIHLDEEGHVEEAYFHVTQFRGFEKFCEGRPYYELPSLMARICGICPVSHLIASAKACDAI
LAVRIPDAAIRLRRVLNLAQILQSHALSFFYLSSPDLLLGMDADPADRNIFGVLRANPAVAKDGIRLRQFGQQVIEWLAG
KRIHPAWVVPGGVSNPLTAENRDRILAGIPEVMAIATRTLDWFKSELEGFREEIRSFANFPTLFMGLVGEDGGLEHYNGK
LRLVDAGGHIVADLPATRFPEYLAEIAHPWTYLKTSFYKPLGYPDGVYRVGPLARLNVIDGIGTPRASQEWAEFRELDRG
PVLSSFYFHYARLIELLYAVEKIEMLLTHADALDSNVRAHASVNNSEGIGVSEAPRGTLMHHYKVDRDGLITWANLIIAT
GHNNAAMNRGVLQVARHFVHGNKLTQGMLNRVEAVIRTFDPCLSCSTHSDGRLALEVELRSHEGEMLDRCRTL

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=144, Percent_Identity=31.25, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1787207, Length=125, Percent_Identity=30.4, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.1.2 [H]

Molecular weight: Translated: 52986; Mature: 52854

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRQKIVIDPVTRIEGHSKITIHLDEEGHVEEAYFHVTQFRGFEKFCEGRPYYELPSLMA
CCCCCEEECCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHH
RICGICPVSHLIASAKACDAILAVRIPDAAIRLRRVLNLAQILQSHALSFFYLSSPDLLL
HHHCCCHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEE
GMDADPADRNIFGVLRANPAVAKDGIRLRQFGQQVIEWLAGKRIHPAWVVPGGVSNPLTA
ECCCCCCCCCEEEEEECCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCC
ENRDRILAGIPEVMAIATRTLDWFKSELEGFREEIRSFANFPTLFMGLVGEDGGLEHYNG
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEECC
KLRLVDAGGHIVADLPATRFPEYLAEIAHPWTYLKTSFYKPLGYPDGVYRVGPLARLNVI
EEEEEECCCEEEECCCHHCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCEECCCCCEEEHH
DGIGTPRASQEWAEFRELDRGPVLSSFYFHYARLIELLYAVEKIEMLLTHADALDSNVRA
HCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
HASVNNSEGIGVSEAPRGTLMHHYKVDRDGLITWANLIIATGHNNAAMNRGVLQVARHFV
EEECCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHH
HGNKLTQGMLNRVEAVIRTFDPCLSCSTHSDGRLALEVELRSHEGEMLDRCRTL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHCCC
>Mature Secondary Structure 
PRQKIVIDPVTRIEGHSKITIHLDEEGHVEEAYFHVTQFRGFEKFCEGRPYYELPSLMA
CCCCEEECCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHH
RICGICPVSHLIASAKACDAILAVRIPDAAIRLRRVLNLAQILQSHALSFFYLSSPDLLL
HHHCCCHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEE
GMDADPADRNIFGVLRANPAVAKDGIRLRQFGQQVIEWLAGKRIHPAWVVPGGVSNPLTA
ECCCCCCCCCEEEEEECCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCC
ENRDRILAGIPEVMAIATRTLDWFKSELEGFREEIRSFANFPTLFMGLVGEDGGLEHYNG
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEECC
KLRLVDAGGHIVADLPATRFPEYLAEIAHPWTYLKTSFYKPLGYPDGVYRVGPLARLNVI
EEEEEECCCEEEECCCHHCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCEECCCCCEEEHH
DGIGTPRASQEWAEFRELDRGPVLSSFYFHYARLIELLYAVEKIEMLLTHADALDSNVRA
HCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
HASVNNSEGIGVSEAPRGTLMHHYKVDRDGLITWANLIIATGHNNAAMNRGVLQVARHFV
EEECCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHH
HGNKLTQGMLNRVEAVIRTFDPCLSCSTHSDGRLALEVELRSHEGEMLDRCRTL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2188945; 12948488; 2496982 [H]