Click here to switch to the map view.

The map label for this gene is hprA [H]

Identifier: 116623800

GI number: 116623800

Start: 5964550

End: 5965506

Strand: Reverse

Name: hprA [H]

Synonym: Acid_4712

Alternate gene names: 116623800

Gene position: 5965506-5964550 (Counterclockwise)

Preceding gene: 116623801

Following gene: 116623799

Centisome position: 59.86

GC content: 61.55

Gene sequence:

>957_bases
ATGAAGATTGTGGTGCTGGACGGATATTGTCTGAATCCCGGCGATCTTTCGTGGGACACGTTGCGCGGGTTTGGCGAGGT
CGAGATTTTCGACCGCACTCCCGCCGATCAGGTCGCCGCGCGCGCCAGGGGCGCTGCCATCGCGCTCACCAATAAGACGC
CGTTCAAGGCCGATACCCTCGCCGGGCTGCCCGATTTGAAATACATCGGCGTGCTGGCCACTGGCTACAACATCGTGGAC
GTAGAAGCCGCACGCCGCTACGGCATTACGGTCACCAATATTCCGACTTACGGCACCGCATCGGTCGCGCAGTTTGTGTT
CGCGCTCCTGCTGGAGATGTGCCACAACGTCAGGCTCCACGCCGATGCCGTGCGGGCCGGCGAGTGGTCGCGCAACGCCG
ATTGGTCGTTCTGGAAATCGCCGCTCGTGGAACTGGCGGGAAAGACAATGGGCGTAATCGGCTTTGGGCGGATCGGCCGC
TCGGTGGGCCGCATCGCCGACGCCATGGGGATGAAGGTGATCGCCAACGATACCTTTCAGGGCAATCCGCCGGACTACGC
GGAATTTCGCTGGGCTACGGTCGAAGAACTGCTCCGTGAAAGCGACGTGGTCAGCCTGCATTCGCCGCTCTTTCCGGAAA
CCCAGGGGATGATCAACGCGCGCAGTCTCGGGTTGATGAAGCCCTCCGCGTTTTTGATCAATACGTCGCGCGGTCCGCTA
GTGGTGGATCAGGATCTGGCCGACGCTCTCAATGCCGGCACCATCGCCGGCGCCGCGCTCGATGTACTCAGCGTCGAGCC
TCCGGCGGAGAGCAATCCGCTGCTCTCGGCGCGCAATTGCCTGGTCACTCCGCACATCGCCTGGGCCACCCGCGAAGCCC
GCGCGCGGCTGATGGATCTGGCCATGAGTAACATTTCAGGTTATTTAAGCGGAAATCCCCTGAATGTGATAAAGTAA

Upstream 100 bases:

>100_bases
GAGCGTGCACGATCTCGACGCCGGACCCGGCTTCGCCGCCGCTGTCGCCGCGCCCGGCGAAGGCTACTCGGTGGCGGTTT
ACGATTACGGAGAAGACTAG

Downstream 100 bases:

>100_bases
CGGTCGGTAGCGCTAAAGGTAACGTATGGATCGACGAAATTTCTTCATCAGCACGGCGGCCGCGGCAGCCGCCTCCACCA
CCGTTTTTGGCAGCCCGAGC

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKIVVLDGYCLNPGDLSWDTLRGFGEVEIFDRTPADQVAARARGAAIALTNKTPFKADTLAGLPDLKYIGVLATGYNIVD
VEAARRYGITVTNIPTYGTASVAQFVFALLLEMCHNVRLHADAVRAGEWSRNADWSFWKSPLVELAGKTMGVIGFGRIGR
SVGRIADAMGMKVIANDTFQGNPPDYAEFRWATVEELLRESDVVSLHSPLFPETQGMINARSLGLMKPSAFLINTSRGPL
VVDQDLADALNAGTIAGAALDVLSVEPPAESNPLLSARNCLVTPHIAWATREARARLMDLAMSNISGYLSGNPLNVIK

Sequences:

>Translated_318_residues
MKIVVLDGYCLNPGDLSWDTLRGFGEVEIFDRTPADQVAARARGAAIALTNKTPFKADTLAGLPDLKYIGVLATGYNIVD
VEAARRYGITVTNIPTYGTASVAQFVFALLLEMCHNVRLHADAVRAGEWSRNADWSFWKSPLVELAGKTMGVIGFGRIGR
SVGRIADAMGMKVIANDTFQGNPPDYAEFRWATVEELLRESDVVSLHSPLFPETQGMINARSLGLMKPSAFLINTSRGPL
VVDQDLADALNAGTIAGAALDVLSVEPPAESNPLLSARNCLVTPHIAWATREARARLMDLAMSNISGYLSGNPLNVIK
>Mature_318_residues
MKIVVLDGYCLNPGDLSWDTLRGFGEVEIFDRTPADQVAARARGAAIALTNKTPFKADTLAGLPDLKYIGVLATGYNIVD
VEAARRYGITVTNIPTYGTASVAQFVFALLLEMCHNVRLHADAVRAGEWSRNADWSFWKSPLVELAGKTMGVIGFGRIGR
SVGRIADAMGMKVIANDTFQGNPPDYAEFRWATVEELLRESDVVSLHSPLFPETQGMINARSLGLMKPSAFLINTSRGPL
VVDQDLADALNAGTIAGAALDVLSVEPPAESNPLLSARNCLVTPHIAWATREARARLMDLAMSNISGYLSGNPLNVIK

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=276, Percent_Identity=31.1594202898551, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI6912396, Length=256, Percent_Identity=33.984375, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI145580578, Length=258, Percent_Identity=33.7209302325581, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI4557499, Length=258, Percent_Identity=33.7209302325581, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI145580575, Length=250, Percent_Identity=34.4, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4557497, Length=258, Percent_Identity=31.7829457364341, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI61743967, Length=265, Percent_Identity=31.3207547169811, Blast_Score=122, Evalue=3e-28,
Organism=Escherichia coli, GI1789279, Length=289, Percent_Identity=36.6782006920415, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI87082289, Length=304, Percent_Identity=31.5789473684211, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787645, Length=305, Percent_Identity=30.1639344262295, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788660, Length=282, Percent_Identity=28.7234042553192, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081824, Length=165, Percent_Identity=33.9393939393939, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=303, Percent_Identity=31.3531353135314, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25147481, Length=298, Percent_Identity=30.5369127516779, Blast_Score=118, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=329, Percent_Identity=31.6109422492401, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6322116, Length=295, Percent_Identity=32.5423728813559, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324055, Length=324, Percent_Identity=29.320987654321, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=320, Percent_Identity=28.125, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6324980, Length=194, Percent_Identity=25.7731958762887, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321253, Length=182, Percent_Identity=26.9230769230769, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646446, Length=270, Percent_Identity=34.4444444444444, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24646448, Length=270, Percent_Identity=34.4444444444444, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24646452, Length=270, Percent_Identity=34.4444444444444, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24646450, Length=270, Percent_Identity=34.4444444444444, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI62472511, Length=262, Percent_Identity=35.1145038167939, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI28574286, Length=296, Percent_Identity=30.4054054054054, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19921140, Length=276, Percent_Identity=30.4347826086957, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45552429, Length=266, Percent_Identity=31.5789473684211, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28574284, Length=266, Percent_Identity=31.5789473684211, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45551003, Length=266, Percent_Identity=31.5789473684211, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24585514, Length=266, Percent_Identity=31.5789473684211, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28574282, Length=266, Percent_Identity=31.5789473684211, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28571528, Length=273, Percent_Identity=34.0659340659341, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24585516, Length=284, Percent_Identity=27.8169014084507, Blast_Score=114, Evalue=9e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34288; Mature: 34288

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVVLDGYCLNPGDLSWDTLRGFGEVEIFDRTPADQVAARARGAAIALTNKTPFKADTL
CEEEEEECEECCCCCCCHHHHCCCCCEEEECCCCHHHHHHHHCCCEEEEECCCCCCHHHH
AGLPDLKYIGVLATGYNIVDVEAARRYGITVTNIPTYGTASVAQFVFALLLEMCHNVRLH
CCCCCCCEEEEEECCCEEEEEHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCEEE
ADAVRAGEWSRNADWSFWKSPLVELAGKTMGVIGFGRIGRSVGRIADAMGMKVIANDTFQ
EHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCEEEECCCCC
GNPPDYAEFRWATVEELLRESDVVSLHSPLFPETQGMINARSLGLMKPSAFLINTSRGPL
CCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEHHHCCCCCCCEEEEECCCCCE
VVDQDLADALNAGTIAGAALDVLSVEPPAESNPLLSARNCLVTPHIAWATREARARLMDL
EECHHHHHHHCCCHHHHHHHHEEECCCCCCCCCCEECCCEEECCCHHHHHHHHHHHHHHH
AMSNISGYLSGNPLNVIK
HHHHCCCEECCCCCCCCC
>Mature Secondary Structure
MKIVVLDGYCLNPGDLSWDTLRGFGEVEIFDRTPADQVAARARGAAIALTNKTPFKADTL
CEEEEEECEECCCCCCCHHHHCCCCCEEEECCCCHHHHHHHHCCCEEEEECCCCCCHHHH
AGLPDLKYIGVLATGYNIVDVEAARRYGITVTNIPTYGTASVAQFVFALLLEMCHNVRLH
CCCCCCCEEEEEECCCEEEEEHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCEEE
ADAVRAGEWSRNADWSFWKSPLVELAGKTMGVIGFGRIGRSVGRIADAMGMKVIANDTFQ
EHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCEEEECCCCC
GNPPDYAEFRWATVEELLRESDVVSLHSPLFPETQGMINARSLGLMKPSAFLINTSRGPL
CCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEHHHCCCCCCCEEEEECCCCCE
VVDQDLADALNAGTIAGAALDVLSVEPPAESNPLLSARNCLVTPHIAWATREARARLMDL
EECHHHHHHHCCCHHHHHHHHEEECCCCCCCCCCEECCCEEECCCHHHHHHHHHHHHHHH
AMSNISGYLSGNPLNVIK
HHHHCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]