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The map label for this gene is dapF [H]

Identifier: 116623686

GI number: 116623686

Start: 5813261

End: 5814067

Strand: Reverse

Name: dapF [H]

Synonym: Acid_4598

Alternate gene names: 116623686

Gene position: 5814067-5813261 (Counterclockwise)

Preceding gene: 116623687

Following gene: 116623685

Centisome position: 58.34

GC content: 64.56

Gene sequence:

>807_bases
ATGAAAATTCCTTTCACCAAGACGCACGGCGCTAAGAACGATTTTCTCTTCACGTGGCGGCGGGACGTGCCGCCGGAAAC
GGATCACGAGGCGCTCGCGATCGCCATTTGCGACCGCCATACCGGGATCGGCGCGGATGGCTGGATGCTGGTGGACCCGC
CCGCCGACGCCGAGGCGGAAGGCACGATCCAACTCTACAACTCCGATGGCAGTATCGCCGAGATGTCGGGCAACGGAACG
CGCTGCGCGGCGGCGTTCCTGATCAAGCACGGAATGGCCGCGGGCGTGGTCCGGGTGCGGACCGGCGCGGGGGTCAAGAG
CATGCGCGTGCTGGAGCGTAAGGGGCTCCTCTTCCAGTTCGAGATGAATATGGGGCGCGCGGAAATCGCGGAGAAGCGCT
TTGAGTTGCAGGTTACCGGCGAGCGGCGCGACGTCACGCTGCTGTGGGTCGGCAATCCGCAATGCGCGGTGCCGGTGGAG
CATTTCGATTTCGACTGGCGGACATTGGGCGCCGAGATCGAACGGCATCCGCACTTTCCCAATCGCACGAATGTATCGTT
CGTAAGAGCCGTGGACCAGCACACTATCGACGTGCGGTTTTGGGAGCGCGGGGCGGGGGAGACCATGAGCAGCGGCACGG
GATCCACCGGCGCGGCCGCGATGGCGGTGGCGCGCGGACTTGCGAGTAGTCCGGTGCGCGTGCAGACCCCGGCGGGTGCG
ATGGATCTGCGCTTCGACGGCGATATTTTCCTCACCGGTCCGGCGGAGATTGTGGCCGAAGGCGAGTTTTTCGCGCCGTC
AACTTAA

Upstream 100 bases:

>100_bases
ACGCCGTGATCGGCATGCAGTATGACGCGACGGAGATCATGCAAGGCGTCACGGAAGTTCTGGCCTACGGAACGGCCGTC
GAGGTGGTACCGGCATCCAA

Downstream 100 bases:

>100_bases
TCCGCCGCTATGCGAAAATCGAGTTTGCATGATTGAGCCACAGATTGTCTCGCCGACGGCGACCACGGAAATTCTTGCCG
AGAAGATCAGGACGCGGAAG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKIPFTKTHGAKNDFLFTWRRDVPPETDHEALAIAICDRHTGIGADGWMLVDPPADAEAEGTIQLYNSDGSIAEMSGNGT
RCAAAFLIKHGMAAGVVRVRTGAGVKSMRVLERKGLLFQFEMNMGRAEIAEKRFELQVTGERRDVTLLWVGNPQCAVPVE
HFDFDWRTLGAEIERHPHFPNRTNVSFVRAVDQHTIDVRFWERGAGETMSSGTGSTGAAAMAVARGLASSPVRVQTPAGA
MDLRFDGDIFLTGPAEIVAEGEFFAPST

Sequences:

>Translated_268_residues
MKIPFTKTHGAKNDFLFTWRRDVPPETDHEALAIAICDRHTGIGADGWMLVDPPADAEAEGTIQLYNSDGSIAEMSGNGT
RCAAAFLIKHGMAAGVVRVRTGAGVKSMRVLERKGLLFQFEMNMGRAEIAEKRFELQVTGERRDVTLLWVGNPQCAVPVE
HFDFDWRTLGAEIERHPHFPNRTNVSFVRAVDQHTIDVRFWERGAGETMSSGTGSTGAAAMAVARGLASSPVRVQTPAGA
MDLRFDGDIFLTGPAEIVAEGEFFAPST
>Mature_268_residues
MKIPFTKTHGAKNDFLFTWRRDVPPETDHEALAIAICDRHTGIGADGWMLVDPPADAEAEGTIQLYNSDGSIAEMSGNGT
RCAAAFLIKHGMAAGVVRVRTGAGVKSMRVLERKGLLFQFEMNMGRAEIAEKRFELQVTGERRDVTLLWVGNPQCAVPVE
HFDFDWRTLGAEIERHPHFPNRTNVSFVRAVDQHTIDVRFWERGAGETMSSGTGSTGAAAMAVARGLASSPVRVQTPAGA
MDLRFDGDIFLTGPAEIVAEGEFFAPST

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=277, Percent_Identity=33.2129963898917, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 29219; Mature: 29219

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIPFTKTHGAKNDFLFTWRRDVPPETDHEALAIAICDRHTGIGADGWMLVDPPADAEAE
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCEEECCCCCCCCC
GTIQLYNSDGSIAEMSGNGTRCAAAFLIKHGMAAGVVRVRTGAGVKSMRVLERKGLLFQF
CEEEEECCCCCEEEECCCCCHHHHHHHHHCCCHHCEEEEECCCCHHHHHHHHHCCCEEEE
EMNMGRAEIAEKRFELQVTGERRDVTLLWVGNPQCAVPVEHFDFDWRTLGAEIERHPHFP
ECCCCHHHHHHCEEEEEECCCCCEEEEEEECCCCEEEEHHHCCCCHHHHCCHHHCCCCCC
NRTNVSFVRAVDQHTIDVRFWERGAGETMSSGTGSTGAAAMAVARGLASSPVRVQTPAGA
CCCCEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCE
MDLRFDGDIFLTGPAEIVAEGEFFAPST
EEEEECCCEEEECCHHEEECCCEECCCC
>Mature Secondary Structure
MKIPFTKTHGAKNDFLFTWRRDVPPETDHEALAIAICDRHTGIGADGWMLVDPPADAEAE
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCEEECCCCCCCCC
GTIQLYNSDGSIAEMSGNGTRCAAAFLIKHGMAAGVVRVRTGAGVKSMRVLERKGLLFQF
CEEEEECCCCCEEEECCCCCHHHHHHHHHCCCHHCEEEEECCCCHHHHHHHHHCCCEEEE
EMNMGRAEIAEKRFELQVTGERRDVTLLWVGNPQCAVPVEHFDFDWRTLGAEIERHPHFP
ECCCCHHHHHHCEEEEEECCCCCEEEEEEECCCCEEEEHHHCCCCHHHHCCHHHCCCCCC
NRTNVSFVRAVDQHTIDVRFWERGAGETMSSGTGSTGAAAMAVARGLASSPVRVQTPAGA
CCCCEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCE
MDLRFDGDIFLTGPAEIVAEGEFFAPST
EEEEECCCEEEECCHHEEECCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA