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The map label for this gene is afsK [H]

Identifier: 116623623

GI number: 116623623

Start: 5732633

End: 5733919

Strand: Direct

Name: afsK [H]

Synonym: Acid_4533

Alternate gene names: 116623623

Gene position: 5732633-5733919 (Clockwise)

Preceding gene: 116623622

Following gene: 116623635

Centisome position: 57.52

GC content: 64.41

Gene sequence:

>1287_bases
ATGCGCCTCTCCCTCACCGTACTGGCTGCGGCGGCCTGCTTCGGGCAAGCGACATTCCGCGGTGATGCGGCGCACTCCGG
CGCGTTCTCCGGCGAAGGGCCGCGCGCGTTCCATGGCGTGAAGTGGAAGTTCGCGACCGGAGGCCGGGTTGTCTCTTCGG
CGGTGGCAAGCAACGGCGCCATCTACTTCGGCAGCGACGATGGCAACATCTACGCGGTCGATCCCGCGAGCGGCAGGCAA
CTCTGGCAATTCACTACCGGCGGACCCATCGATTCCACTCCCGCGATTGCCGGCGGCGTGGTCTATTTCGGCAGCTACGA
TGCCAAGTTTTACGCCGTGGACGCCAAATCGGGCGCGCTGCGGTGGAAGTTCGCAGCTGGCAGTGAGCGGCGCTTCGAAG
CCAGGGGCCTGCACGGCATGCAGCCCTTAACGCAGACCTTCCTCGATCCTTACGACGTTTTTCTGTCCAGCCCGGTGGTG
GCCGAAGGCGCAGTCTACTTCGGCTCCGGCGATGGCAACCTCTACGCGCTTGACGCCGCCTCGGGCGCCCTGCGGTGGAA
GTTCCATACCGGCGACGTAGTTCACGCCTCCCCCGCCTACGCCGCCGGGACCCTCTTCTTCGGCAGTTGGGACAGCCGCT
TCTACGCCGTCGAGGCCACCACCGGGAAGGAGAAGTGGCGCTTCCAGGCCGGCACAGACGACCTGACCCACAACCAGATT
GGCTTTCAATCTTCACCGGCCGTGGTGGATGGCGTGGTTTACACCGGCTGCCGCGATTCCAACGTCTACGCGCTCGATGC
CGCCACCGGCAAGGAGAAATGGCGCGTCAACAATCAGGGCAGCTGGGTGAACAGCTCGCCCGCCGTCGCCGAGGGCAAAG
TGTACTTCGCGACGGCGGACTCCAGCCTCTTCCACGCGGTGGATGCGGCCACGGGCAAGTCCGCATTCAAGCACCAGCTG
AAGGGCTACGTCTTTTCCTCGCCCTCGGTCGCGGGAGACGTGGTCTACCTGGGGATTCTCAACGGCACGATGGAAGCGCG
CGATCGCAAGACCGGCGAGTTACTGTGGACCTTCCAGACCGAGACGTCCAAACAGAATCGCGACTGGGTGCTGACCGCGG
AGGGCAAGCTGAATCCCGCGATGTTCTTCCGTTCGAACTGGCTGGAGGGTGGAATTCTGGCCGCCAATCGCGAGTTCGCG
ATCGGCTCTATTTTCAGCGCGCCGCTGATCTCGGACGGCACGGTCTACGTGGGCAGCGCCGACGGCTATCTCTACGCCCT
GCAGTAG

Upstream 100 bases:

>100_bases
GCATCAGCTTCGAAGGGCGGTGGCAGAAAGACGAATTGAACGGCACGTTCGCGCAGGGTCCGATCGAAAGCCCGCTGAGC
TTACGGAGGGCGCGGTGAGG

Downstream 100 bases:

>100_bases
CGCGGGGCCGGCTTCTTCCGCCCGCCCCGTCCGCCGCAAATTTGGCAGGCCGGAGGAGCCTGCCCAACGCAGTCCGGGGT
TGTGCTGGCGTCATCCCGCG

Product: pyrrolo-quinoline quinone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MRLSLTVLAAAACFGQATFRGDAAHSGAFSGEGPRAFHGVKWKFATGGRVVSSAVASNGAIYFGSDDGNIYAVDPASGRQ
LWQFTTGGPIDSTPAIAGGVVYFGSYDAKFYAVDAKSGALRWKFAAGSERRFEARGLHGMQPLTQTFLDPYDVFLSSPVV
AEGAVYFGSGDGNLYALDAASGALRWKFHTGDVVHASPAYAAGTLFFGSWDSRFYAVEATTGKEKWRFQAGTDDLTHNQI
GFQSSPAVVDGVVYTGCRDSNVYALDAATGKEKWRVNNQGSWVNSSPAVAEGKVYFATADSSLFHAVDAATGKSAFKHQL
KGYVFSSPSVAGDVVYLGILNGTMEARDRKTGELLWTFQTETSKQNRDWVLTAEGKLNPAMFFRSNWLEGGILAANREFA
IGSIFSAPLISDGTVYVGSADGYLYALQ

Sequences:

>Translated_428_residues
MRLSLTVLAAAACFGQATFRGDAAHSGAFSGEGPRAFHGVKWKFATGGRVVSSAVASNGAIYFGSDDGNIYAVDPASGRQ
LWQFTTGGPIDSTPAIAGGVVYFGSYDAKFYAVDAKSGALRWKFAAGSERRFEARGLHGMQPLTQTFLDPYDVFLSSPVV
AEGAVYFGSGDGNLYALDAASGALRWKFHTGDVVHASPAYAAGTLFFGSWDSRFYAVEATTGKEKWRFQAGTDDLTHNQI
GFQSSPAVVDGVVYTGCRDSNVYALDAATGKEKWRVNNQGSWVNSSPAVAEGKVYFATADSSLFHAVDAATGKSAFKHQL
KGYVFSSPSVAGDVVYLGILNGTMEARDRKTGELLWTFQTETSKQNRDWVLTAEGKLNPAMFFRSNWLEGGILAANREFA
IGSIFSAPLISDGTVYVGSADGYLYALQ
>Mature_428_residues
MRLSLTVLAAAACFGQATFRGDAAHSGAFSGEGPRAFHGVKWKFATGGRVVSSAVASNGAIYFGSDDGNIYAVDPASGRQ
LWQFTTGGPIDSTPAIAGGVVYFGSYDAKFYAVDAKSGALRWKFAAGSERRFEARGLHGMQPLTQTFLDPYDVFLSSPVV
AEGAVYFGSGDGNLYALDAASGALRWKFHTGDVVHASPAYAAGTLFFGSWDSRFYAVEATTGKEKWRFQAGTDDLTHNQI
GFQSSPAVVDGVVYTGCRDSNVYALDAATGKEKWRVNNQGSWVNSSPAVAEGKVYFATADSSLFHAVDAATGKSAFKHQL
KGYVFSSPSVAGDVVYLGILNGTMEARDRKTGELLWTFQTETSKQNRDWVLTAEGKLNPAMFFRSNWLEGGILAANREFA
IGSIFSAPLISDGTVYVGSADGYLYALQ

Specific function: Involved in the regulation of secondary metabolism by phosphorylating, on both Ser and Thr, the AfsR global regulatory protein involved in the control of secondary metabolism [H]

COG id: COG1520

COG function: function code S; FOG: WD40-like repeat

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 protein kinase domain [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=401, Percent_Identity=23.1920199501247, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1788859, Length=281, Percent_Identity=21.7081850533808, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR018391
- InterPro:   IPR002372
- InterPro:   IPR000719
- InterPro:   IPR017441
- InterPro:   IPR011047
- InterPro:   IPR017442
- InterPro:   IPR008271 [H]

Pfam domain/function: PF00069 Pkinase; PF01011 PQQ [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 45710; Mature: 45710

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSLTVLAAAACFGQATFRGDAAHSGAFSGEGPRAFHGVKWKFATGGRVVSSAVASNGA
CEEEEHHHHHHHHHCCCEECCCCCCCCCCCCCCCCEECCEEEEEECCCHHHHHHHHCCCE
IYFGSDDGNIYAVDPASGRQLWQFTTGGPIDSTPAIAGGVVYFGSYDAKFYAVDAKSGAL
EEEECCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCE
RWKFAAGSERRFEARGLHGMQPLTQTFLDPYDVFLSSPVVAEGAVYFGSGDGNLYALDAA
EEEEECCCCCEEHHCCCCCCCHHHHHHCCHHHHHHCCCCEECCEEEEECCCCCEEEEECC
SGALRWKFHTGDVVHASPAYAAGTLFFGSWDSRFYAVEATTGKEKWRFQAGTDDLTHNQI
CCEEEEEEECCCEEECCCCCEEEEEEEECCCCEEEEEEECCCCCEEEEECCCCCCCCCCC
GFQSSPAVVDGVVYTGCRDSNVYALDAATGKEKWRVNNQGSWVNSSPAVAEGKVYFATAD
CCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCEECCEEEEEECC
SSLFHAVDAATGKSAFKHQLKGYVFSSPSVAGDVVYLGILNGTMEARDRKTGELLWTFQT
CCEEEEECCCCCHHHHHHHHCCEEEECCCCCCCEEEEEEECCCCHHCCCCCCEEEEEEEC
ETSKQNRDWVLTAEGKLNPAMFFRSNWLEGGILAANREFAIGSIFSAPLISDGTVYVGSA
CCCCCCCCEEEEECCCCCCEEEEECCCCCCCEEEECCCEEEEEEECCCEECCCEEEEECC
DGYLYALQ
CCEEEEEC
>Mature Secondary Structure
MRLSLTVLAAAACFGQATFRGDAAHSGAFSGEGPRAFHGVKWKFATGGRVVSSAVASNGA
CEEEEHHHHHHHHHCCCEECCCCCCCCCCCCCCCCEECCEEEEEECCCHHHHHHHHCCCE
IYFGSDDGNIYAVDPASGRQLWQFTTGGPIDSTPAIAGGVVYFGSYDAKFYAVDAKSGAL
EEEECCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCE
RWKFAAGSERRFEARGLHGMQPLTQTFLDPYDVFLSSPVVAEGAVYFGSGDGNLYALDAA
EEEEECCCCCEEHHCCCCCCCHHHHHHCCHHHHHHCCCCEECCEEEEECCCCCEEEEECC
SGALRWKFHTGDVVHASPAYAAGTLFFGSWDSRFYAVEATTGKEKWRFQAGTDDLTHNQI
CCEEEEEEECCCEEECCCCCEEEEEEEECCCCEEEEEEECCCCCEEEEECCCCCCCCCCC
GFQSSPAVVDGVVYTGCRDSNVYALDAATGKEKWRVNNQGSWVNSSPAVAEGKVYFATAD
CCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCEECCEEEEEECC
SSLFHAVDAATGKSAFKHQLKGYVFSSPSVAGDVVYLGILNGTMEARDRKTGELLWTFQT
CCEEEEECCCCCHHHHHHHHCCEEEECCCCCCCEEEEEEECCCCHHCCCCCCEEEEEEEC
ETSKQNRDWVLTAEGKLNPAMFFRSNWLEGGILAANREFAIGSIFSAPLISDGTVYVGSA
CCCCCCCCEEEEECCCCCCEEEEECCCCCCCEEEECCCEEEEEEECCCEECCCEEEEECC
DGYLYALQ
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8063104; 8635757; 12000953 [H]