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The map label for this gene is gph [H]

Identifier: 116623547

GI number: 116623547

Start: 5644029

End: 5644694

Strand: Reverse

Name: gph [H]

Synonym: Acid_4457

Alternate gene names: 116623547

Gene position: 5644694-5644029 (Counterclockwise)

Preceding gene: 116623552

Following gene: 116623545

Centisome position: 56.64

GC content: 66.97

Gene sequence:

>666_bases
ATGCCTGCATTCCTCTTCGACCTTGACGGCACCCTGGTGGACAGTGTCTATCAACACGTGCTCGCCTGGCGCGACGCGCT
CGAGATCGCCGGACTTCCGCTCTCTGTCTGGCGCATACACCGGCGCATCGGAATGAGCGGCGGCCTGTTCGTGAATGCGC
TCGCCCGCGAAACGGGTCATAGACTCAGCGAAGGCGAGGTCGCCCGCATGCTCGAGCTGCACAGCGAGGCGTACCGCAAT
TACGCGGGACAGGTGCGGCCTCTTCCTGGCGCGGCGGCGCTGTTGGCCTATCTGGCGCAGCAAAAAGTTCCCCACGCCAT
CGCGACCAGCGGCTACCGCGATAGCGCCGCGCCGGCGCTGGCCGCGCTCGGAGTCGATCCGAATCTCCCCATCGTGACCC
GCGATCAGGTGGCGCATGCCAAGCCCGATCCCGATCTCTTCCTCGCCGCGGCGGACCGGCTTGGCGTGGCGATCGAAGAT
TCCGTTGTCGTCGGCGACAGCATATGGGATCTGCTCGCCGCGCGGCGCGCCCGCGCCCTCAGCATCGGCCTGCTCTCGGG
CGGATACGGCGAAGAGGAGTTGGAGCGCGCCGGCGCCTATCGAATTTATCAGGACCCTGCCGACCTTCTGGCCCATCTGG
ATGAGGTCGGCGTCCGCGGGCGCTAG

Upstream 100 bases:

>100_bases
GGAAACGGCTGCGATCCACGCGATTGCGCATAAGAGCAGAAAACGCATAAAGGAAGCGACAGGGCTCCGCAAGAATTATA
TCAGTGAGAGAAACTAAAGC

Downstream 100 bases:

>100_bases
CCGATGCTGGGCATCCTGGTTCATGGAGACAATCACTTCATCGTGCGCGGCCCCTGCCCGGACCGTGACACGGCATACGC
CCTGGTGCAACACTGGTCCC

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MPAFLFDLDGTLVDSVYQHVLAWRDALEIAGLPLSVWRIHRRIGMSGGLFVNALARETGHRLSEGEVARMLELHSEAYRN
YAGQVRPLPGAAALLAYLAQQKVPHAIATSGYRDSAAPALAALGVDPNLPIVTRDQVAHAKPDPDLFLAAADRLGVAIED
SVVVGDSIWDLLAARRARALSIGLLSGGYGEEELERAGAYRIYQDPADLLAHLDEVGVRGR

Sequences:

>Translated_221_residues
MPAFLFDLDGTLVDSVYQHVLAWRDALEIAGLPLSVWRIHRRIGMSGGLFVNALARETGHRLSEGEVARMLELHSEAYRN
YAGQVRPLPGAAALLAYLAQQKVPHAIATSGYRDSAAPALAALGVDPNLPIVTRDQVAHAKPDPDLFLAAADRLGVAIED
SVVVGDSIWDLLAARRARALSIGLLSGGYGEEELERAGAYRIYQDPADLLAHLDEVGVRGR
>Mature_220_residues
PAFLFDLDGTLVDSVYQHVLAWRDALEIAGLPLSVWRIHRRIGMSGGLFVNALARETGHRLSEGEVARMLELHSEAYRNY
AGQVRPLPGAAALLAYLAQQKVPHAIATSGYRDSAAPALAALGVDPNLPIVTRDQVAHAKPDPDLFLAAADRLGVAIEDS
VVVGDSIWDLLAARRARALSIGLLSGGYGEEELERAGAYRIYQDPADLLAHLDEVGVRGR

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789046, Length=188, Percent_Identity=31.9148936170213, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1789787, Length=228, Percent_Identity=29.3859649122807, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23821; Mature: 23690

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAFLFDLDGTLVDSVYQHVLAWRDALEIAGLPLSVWRIHRRIGMSGGLFVNALARETGH
CCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCC
RLSEGEVARMLELHSEAYRNYAGQVRPLPGAAALLAYLAQQKVPHAIATSGYRDSAAPAL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCHHH
AALGVDPNLPIVTRDQVAHAKPDPDLFLAAADRLGVAIEDSVVVGDSIWDLLAARRARAL
HHCCCCCCCCEEECCCHHCCCCCCCEEEEEHHHCCEEEECCEEECHHHHHHHHHHHHHHH
SIGLLSGGYGEEELERAGAYRIYQDPADLLAHLDEVGVRGR
EEEECCCCCCHHHHHHCCCCEEECCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PAFLFDLDGTLVDSVYQHVLAWRDALEIAGLPLSVWRIHRRIGMSGGLFVNALARETGH
CCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCC
RLSEGEVARMLELHSEAYRNYAGQVRPLPGAAALLAYLAQQKVPHAIATSGYRDSAAPAL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCHHH
AALGVDPNLPIVTRDQVAHAKPDPDLFLAAADRLGVAIEDSVVVGDSIWDLLAARRARAL
HHCCCCCCCCEEECCCHHCCCCCCCEEEEEHHHCCEEEECCEEECHHHHHHHHHHHHHHH
SIGLLSGGYGEEELERAGAYRIYQDPADLLAHLDEVGVRGR
EEEECCCCCCHHHHHHCCCCEEECCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]