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The map label for this gene is macA [H]

Identifier: 116623245

GI number: 116623245

Start: 5267047

End: 5268180

Strand: Reverse

Name: macA [H]

Synonym: Acid_4152

Alternate gene names: 116623245

Gene position: 5268180-5267047 (Counterclockwise)

Preceding gene: 116623246

Following gene: 116623244

Centisome position: 52.86

GC content: 67.46

Gene sequence:

>1134_bases
ATGACTCGATATCTCACCCTTCTCCCTGTCCTGCTTCTCGCCTCCTGCGGCGGCGATCCGCCCCATCCGGCCATCGCGCT
ACAACCCGCGCCCGTCCCCGTGGGGATCGCCACCGTCGCAACCCAGGATTGGCCAGCCGCCTACGAGGCCACAGGCACCG
TCCGCGCGCGCACCACCGCCGTGCTTGCCAGCAAGGTGATGGGCTACGTCCTCAAGGTCGGAGCCCAGACCGGCGATCAC
GTTCGTCAGGGCCAGCCCCTCGTCACCCTCGACTCCCGCGATCTCGATGTCAACGTCCGCCGTGCCGACGCAGGCCGCGC
TGAAATCCTCAGCGCCGTACCGGAGCTCGACCATGCCACCGCCGCAGCCAAAGCCAATCTCGATCTCGCCCAGGCCACCT
TCCACCGCATGGAGGAACTCGCCGCCAAAAAGTCCACCTCCAACCAGGAGTTCGACGAAGCCTCCGCCCGCCTCAAGTCG
GCCCAGGCCAACTACGAAATGGCCCGCGCACGCCGCGCCCAAGTCAACGCCAGGCTTGCGCAGGTCGATCAGGATGCCAG
TGCCGCCGCCATCGTGCGCGATTACGCGACCCTCTCCGCTCCCTTTGCCGGTGTCGTGATCGCTCGCTCCGTCGAACCCG
GCAATCTTGCCTCCCCCGGCGTCCCCCTGCTCACGCTGGAACAGGACGGCCTCTACCGCCTCGAAGTCGCGGTCGATGAA
TCCAAACTTTCCGCCGTCAAGACCGGCCAGTCCGTCGAGACCATGGTCGAGGTTTCCAGCCGCGCGCGTGTCGCCGAAAT
CGTTCCCGCGATCGACCCCGCCTCCCGGACTTACATCGTCAAACTCGATCTTCCTCCCGCGCCGCATCTGCGCACCGGCA
TGTTCGGCCGCGCCTTCTTCCCGCTCGCCCCACGCAAGGTCGTCGGCATTCCCGCCGCCGCCCTGATCGAGCGTGGCCAG
CTTCAATCCGTGTTCGTCGTCGAGTCGGGCGTCGCCCGCACCCGCATCCTCACCACCGGTGGGCGCTGTGACGACTCCGT
CGAAGTGCTCTCCGGTCTCAACCCTGGCGAAAAGGTCGTCTCTCCGGTGCCGCCCGCACTGCAGGACGGCGCCCGAGTCG
AGGTCCGCCCGTGA

Upstream 100 bases:

>100_bases
GTACTCACTATCTCGCCGCCATCCACGATCAGCGCGTCGCGGCCACCATGCTCGAACTTGCCGCGGGACGCCTCAATGCC
GATTCGGAGGCCCTCAACTA

Downstream 100 bases:

>100_bases
ACTCGCCCCGACCCCTGGGTCCCGCCGGCCGCCTCGCCCATGCCTGGATCTCCTCCAAGCTCACGCCTCTCCTCATTTGC
GCCGCTCTCCTCATCGGCGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MTRYLTLLPVLLLASCGGDPPHPAIALQPAPVPVGIATVATQDWPAAYEATGTVRARTTAVLASKVMGYVLKVGAQTGDH
VRQGQPLVTLDSRDLDVNVRRADAGRAEILSAVPELDHATAAAKANLDLAQATFHRMEELAAKKSTSNQEFDEASARLKS
AQANYEMARARRAQVNARLAQVDQDASAAAIVRDYATLSAPFAGVVIARSVEPGNLASPGVPLLTLEQDGLYRLEVAVDE
SKLSAVKTGQSVETMVEVSSRARVAEIVPAIDPASRTYIVKLDLPPAPHLRTGMFGRAFFPLAPRKVVGIPAAALIERGQ
LQSVFVVESGVARTRILTTGGRCDDSVEVLSGLNPGEKVVSPVPPALQDGARVEVRP

Sequences:

>Translated_377_residues
MTRYLTLLPVLLLASCGGDPPHPAIALQPAPVPVGIATVATQDWPAAYEATGTVRARTTAVLASKVMGYVLKVGAQTGDH
VRQGQPLVTLDSRDLDVNVRRADAGRAEILSAVPELDHATAAAKANLDLAQATFHRMEELAAKKSTSNQEFDEASARLKS
AQANYEMARARRAQVNARLAQVDQDASAAAIVRDYATLSAPFAGVVIARSVEPGNLASPGVPLLTLEQDGLYRLEVAVDE
SKLSAVKTGQSVETMVEVSSRARVAEIVPAIDPASRTYIVKLDLPPAPHLRTGMFGRAFFPLAPRKVVGIPAAALIERGQ
LQSVFVVESGVARTRILTTGGRCDDSVEVLSGLNPGEKVVSPVPPALQDGARVEVRP
>Mature_376_residues
TRYLTLLPVLLLASCGGDPPHPAIALQPAPVPVGIATVATQDWPAAYEATGTVRARTTAVLASKVMGYVLKVGAQTGDHV
RQGQPLVTLDSRDLDVNVRRADAGRAEILSAVPELDHATAAAKANLDLAQATFHRMEELAAKKSTSNQEFDEASARLKSA
QANYEMARARRAQVNARLAQVDQDASAAAIVRDYATLSAPFAGVVIARSVEPGNLASPGVPLLTLEQDGLYRLEVAVDES
KLSAVKTGQSVETMVEVSSRARVAEIVPAIDPASRTYIVKLDLPPAPHLRTGMFGRAFFPLAPRKVVGIPAAALIERGQL
QSVFVVESGVARTRILTTGGRCDDSVEVLSGLNPGEKVVSPVPPALQDGARVEVRP

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=327, Percent_Identity=25.3822629969419, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39835; Mature: 39704

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYLTLLPVLLLASCGGDPPHPAIALQPAPVPVGIATVATQDWPAAYEATGTVRARTTA
CCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHH
VLASKVMGYVLKVGAQTGDHVRQGQPLVTLDSRDLDVNVRRADAGRAEILSAVPELDHAT
HHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCCEEEEEECCCCHHHHHHHCCCHHHHH
AAAKANLDLAQATFHRMEELAAKKSTSNQEFDEASARLKSAQANYEMARARRAQVNARLA
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVDQDASAAAIVRDYATLSAPFAGVVIARSVEPGNLASPGVPLLTLEQDGLYRLEVAVDE
HCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCEEEEEEEECH
SKLSAVKTGQSVETMVEVSSRARVAEIVPAIDPASRTYIVKLDLPPAPHLRTGMFGRAFF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHCCCCCCEEC
PLAPRKVVGIPAAALIERGQLQSVFVVESGVARTRILTTGGRCDDSVEVLSGLNPGEKVV
CCCCCCEECCCHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHCCCCCHHHC
SPVPPALQDGARVEVRP
CCCCHHHCCCCEEEECC
>Mature Secondary Structure 
TRYLTLLPVLLLASCGGDPPHPAIALQPAPVPVGIATVATQDWPAAYEATGTVRARTTA
CHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHH
VLASKVMGYVLKVGAQTGDHVRQGQPLVTLDSRDLDVNVRRADAGRAEILSAVPELDHAT
HHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCCEEEEEECCCCHHHHHHHCCCHHHHH
AAAKANLDLAQATFHRMEELAAKKSTSNQEFDEASARLKSAQANYEMARARRAQVNARLA
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVDQDASAAAIVRDYATLSAPFAGVVIARSVEPGNLASPGVPLLTLEQDGLYRLEVAVDE
HCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCEEEEEEEECH
SKLSAVKTGQSVETMVEVSSRARVAEIVPAIDPASRTYIVKLDLPPAPHLRTGMFGRAFF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHCCCCCCEEC
PLAPRKVVGIPAAALIERGQLQSVFVVESGVARTRILTTGGRCDDSVEVLSGLNPGEKVV
CCCCCCEECCCHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHCCCCCHHHC
SPVPPALQDGARVEVRP
CCCCHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]