Click here to switch to the map view.

The map label for this gene is pgl [H]

Identifier: 116623081

GI number: 116623081

Start: 5053281

End: 5054702

Strand: Reverse

Name: pgl [H]

Synonym: Acid_3985

Alternate gene names: 116623081

Gene position: 5054702-5053281 (Counterclockwise)

Preceding gene: 116623083

Following gene: 116623080

Centisome position: 50.72

GC content: 63.85

Gene sequence:

>1422_bases
TTGTACACCGTGTATAGTATCGGTTTGACCGACCCGATGGAACATCTACTGAGCCGCCGCGCGGCCCTGCGATTGACGCC
CGCGCTGCTCCTGGCCCGTTCCGGCCGCGCGCAGACCAAAATCTTCGACGTACGCGATTACGGGGCCAAAGGCGATGGGG
TCACGCTCGATACCGCCGCCATCCAGCGCGCGATCGATGAAGCCGCCGCCGAAGGCAAAGGCGCCCAGGTGCTGGTGCGC
GGCGGGAAGAAGTACCTGATCGGCACTTTGGAATTGAAGGGCGCAATCGACTTTCACCTCGCCGACGACGCCGAACTCGT
AGTCACCGCCGATCCGGCGCAATATCGCGGCGCAGCCGTGATCGCGGCCACTGGCGCCGAAGGGTTGCGTATCACCGGCA
CCGGCAACATCAACGGCCGCGCGCGTGAATTCATGACGGGCTACGACCAACCGGGCGAGTGGTGGCTGCCCAAAACCTTC
CGCCCGAAGATGTTCGTGCTCACCGCCTGTAAGGGTCTCGAGGTCCACGGCATCAGCTTCAGTGAGGCGCCCGAATGGGG
CCTGCACATGGTGGGCTGCGAACACGTGCTGGTGGATGGCCTGCGCATACGCAACCTGCTCGACGTCCCCAACTGCGACG
GCATCGACCCCGACCATTGCCGCGACGTGGAGATCAAGAACTGCGACATCGTGTGCGGCGACGATGCGATCGTGGTCAAG
AGCACGCGCCAGGAGCGCGATTTCGGCGCCTGCTCCGGCATACGAGTACACGATTGCCTGATTCAGACGCAGGATTGCGG
CGTGAAGATCGGGACCGAGACCACCCAGGAGATCCACGACGTGGTCTTCGAACGCTGCGAGATCCGCTCCAGCTCGCGCG
GCATCGGTATTCAACTGCGAGATGAAGGCGCTGTCCACAATATCGAATTCCGGGACATCAAGCTGGTGTCGCGCTACCAC
TCCGACCCCTGGTGGGGCCGCGGCGAGGCGATATCGCTGACCGTGCATCCACGCAATCAGCAGACGAAGGTCGGCAAGCT
ATACGACATCCGCATCCGCAATATCACCGCCCGCGCGGAGAACAGCGCCCGCATCTGCGGCTGCCCGGAAAGCCCGATCG
CTGACGTGAAGCTGGAGAACGTCACGCTCACCCTGGACCGCTGGACGAAGTACCCAGGCGGAGTGTGGGACAACCGCCCC
GGCGGTGCCGAAGGCGGGATCGAGCACCACAACACTCCCGGATTCGCGGTTCGCAACGCGCGCAATGTGAGCCTGAGTGG
TTGCTCGGTGGTCTGGGGGACCAAGCCCGCGGAGAATTTCAGTAATGCGCTCGAGGTGGAACAGGTGACGGGCCTCGCGT
TGCGGCGCTTCTCCGGGACGGCGGCGCATCCGGATAACGACCCGGATATCGCGTTGCGTTAG

Upstream 100 bases:

>100_bases
GGCCGGGAATTGGAAAATCTTGAACGTGGTGCCCGGCCGGTCCACGGCAAACGCCGGCAGACTCAGGGCGAAAGCCAGAA
GGAGAACGTATCTCATAGGT

Downstream 100 bases:

>100_bases
CTTTCCTTTCTCGCAGAGGCGCAGAGCCGCAGAGTAACGCGAAGAACACCTTGAAACGCCGGCCTTCCTTTGCGGTTCTC
TGCGGCTCTGCGTCTTTGCG

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MYTVYSIGLTDPMEHLLSRRAALRLTPALLLARSGRAQTKIFDVRDYGAKGDGVTLDTAAIQRAIDEAAAEGKGAQVLVR
GGKKYLIGTLELKGAIDFHLADDAELVVTADPAQYRGAAVIAATGAEGLRITGTGNINGRAREFMTGYDQPGEWWLPKTF
RPKMFVLTACKGLEVHGISFSEAPEWGLHMVGCEHVLVDGLRIRNLLDVPNCDGIDPDHCRDVEIKNCDIVCGDDAIVVK
STRQERDFGACSGIRVHDCLIQTQDCGVKIGTETTQEIHDVVFERCEIRSSSRGIGIQLRDEGAVHNIEFRDIKLVSRYH
SDPWWGRGEAISLTVHPRNQQTKVGKLYDIRIRNITARAENSARICGCPESPIADVKLENVTLTLDRWTKYPGGVWDNRP
GGAEGGIEHHNTPGFAVRNARNVSLSGCSVVWGTKPAENFSNALEVEQVTGLALRRFSGTAAHPDNDPDIALR

Sequences:

>Translated_473_residues
MYTVYSIGLTDPMEHLLSRRAALRLTPALLLARSGRAQTKIFDVRDYGAKGDGVTLDTAAIQRAIDEAAAEGKGAQVLVR
GGKKYLIGTLELKGAIDFHLADDAELVVTADPAQYRGAAVIAATGAEGLRITGTGNINGRAREFMTGYDQPGEWWLPKTF
RPKMFVLTACKGLEVHGISFSEAPEWGLHMVGCEHVLVDGLRIRNLLDVPNCDGIDPDHCRDVEIKNCDIVCGDDAIVVK
STRQERDFGACSGIRVHDCLIQTQDCGVKIGTETTQEIHDVVFERCEIRSSSRGIGIQLRDEGAVHNIEFRDIKLVSRYH
SDPWWGRGEAISLTVHPRNQQTKVGKLYDIRIRNITARAENSARICGCPESPIADVKLENVTLTLDRWTKYPGGVWDNRP
GGAEGGIEHHNTPGFAVRNARNVSLSGCSVVWGTKPAENFSNALEVEQVTGLALRRFSGTAAHPDNDPDIALR
>Mature_473_residues
MYTVYSIGLTDPMEHLLSRRAALRLTPALLLARSGRAQTKIFDVRDYGAKGDGVTLDTAAIQRAIDEAAAEGKGAQVLVR
GGKKYLIGTLELKGAIDFHLADDAELVVTADPAQYRGAAVIAATGAEGLRITGTGNINGRAREFMTGYDQPGEWWLPKTF
RPKMFVLTACKGLEVHGISFSEAPEWGLHMVGCEHVLVDGLRIRNLLDVPNCDGIDPDHCRDVEIKNCDIVCGDDAIVVK
STRQERDFGACSGIRVHDCLIQTQDCGVKIGTETTQEIHDVVFERCEIRSSSRGIGIQLRDEGAVHNIEFRDIKLVSRYH
SDPWWGRGEAISLTVHPRNQQTKVGKLYDIRIRNITARAENSARICGCPESPIADVKLENVTLTLDRWTKYPGGVWDNRP
GGAEGGIEHHNTPGFAVRNARNVSLSGCSVVWGTKPAENFSNALEVEQVTGLALRRFSGTAAHPDNDPDIALR

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 51756; Mature: 51756

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTVYSIGLTDPMEHLLSRRAALRLTPALLLARSGRAQTKIFDVRDYGAKGDGVTLDTAA
CEEEEEECCCCHHHHHHHHHHHHEECHHHEEECCCCCCEEEEEEECCCCCCCCEEEEHHH
IQRAIDEAAAEGKGAQVLVRGGKKYLIGTLELKGAIDFHLADDAELVVTADPAQYRGAAV
HHHHHHHHHHCCCCCEEEEECCCEEEEEEEEECCEEEEEECCCCEEEEEECCHHHCCEEE
IAATGAEGLRITGTGNINGRAREFMTGYDQPGEWWLPKTFRPKMFVLTACKGLEVHGISF
EEECCCCCEEEEECCCCCCCHHHHHCCCCCCCCEECCCCCCCCEEEEEECCCCEEECCCC
SEAPEWGLHMVGCEHVLVDGLRIRNLLDVPNCDGIDPDHCRDVEIKNCDIVCGDDAIVVK
CCCCCCCEEEECHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCEECCCEEEECCCEEEEE
STRQERDFGACSGIRVHDCLIQTQDCGVKIGTETTQEIHDVVFERCEIRSSSRGIGIQLR
CCCCCCCCCCCCCCEEEHEEEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEC
DEGAVHNIEFRDIKLVSRYHSDPWWGRGEAISLTVHPRNQQTKVGKLYDIRIRNITARAE
CCCCEEEEEEHHEEEEHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEEECC
NSARICGCPESPIADVKLENVTLTLDRWTKYPGGVWDNRPGGAEGGIEHHNTPGFAVRNA
CCCEEECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECC
RNVSLSGCSVVWGTKPAENFSNALEVEQVTGLALRRFSGTAAHPDNDPDIALR
CEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEC
>Mature Secondary Structure
MYTVYSIGLTDPMEHLLSRRAALRLTPALLLARSGRAQTKIFDVRDYGAKGDGVTLDTAA
CEEEEEECCCCHHHHHHHHHHHHEECHHHEEECCCCCCEEEEEEECCCCCCCCEEEEHHH
IQRAIDEAAAEGKGAQVLVRGGKKYLIGTLELKGAIDFHLADDAELVVTADPAQYRGAAV
HHHHHHHHHHCCCCCEEEEECCCEEEEEEEEECCEEEEEECCCCEEEEEECCHHHCCEEE
IAATGAEGLRITGTGNINGRAREFMTGYDQPGEWWLPKTFRPKMFVLTACKGLEVHGISF
EEECCCCCEEEEECCCCCCCHHHHHCCCCCCCCEECCCCCCCCEEEEEECCCCEEECCCC
SEAPEWGLHMVGCEHVLVDGLRIRNLLDVPNCDGIDPDHCRDVEIKNCDIVCGDDAIVVK
CCCCCCCEEEECHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCEECCCEEEECCCEEEEE
STRQERDFGACSGIRVHDCLIQTQDCGVKIGTETTQEIHDVVFERCEIRSSSRGIGIQLR
CCCCCCCCCCCCCCEEEHEEEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEC
DEGAVHNIEFRDIKLVSRYHSDPWWGRGEAISLTVHPRNQQTKVGKLYDIRIRNITARAE
CCCCEEEEEEHHEEEEHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEEECC
NSARICGCPESPIADVKLENVTLTLDRWTKYPGGVWDNRPGGAEGGIEHHNTPGFAVRNA
CCCEEECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECC
RNVSLSGCSVVWGTKPAENFSNALEVEQVTGLALRRFSGTAAHPDNDPDIALR
CEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]