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The map label for this gene is hpcH [H]

Identifier: 116623076

GI number: 116623076

Start: 5046839

End: 5047702

Strand: Direct

Name: hpcH [H]

Synonym: Acid_3980

Alternate gene names: 116623076

Gene position: 5046839-5047702 (Clockwise)

Preceding gene: 116623075

Following gene: 116623082

Centisome position: 50.64

GC content: 67.48

Gene sequence:

>864_bases
ATGTCGCAAAATAATGCCGCCCTGCGGCCCAACCAATTCAAGCGCGCCCTTGTCGAAGGCCGCCCTCAGATCGGCCTGTG
GAGTTCCCTGTGCAGCAACATCGCCGCCGAGATCATCTGTAATTCCGGCTTCGATTGGATCGTTGTCGATACCGAGCACG
CCCCCAACGAAGTCCCCGGCCTCGTCGCGCAATTGCAGGCCATGGTCAACGGCACCGCCGAGCCCGTGGTGCGCTGCGCC
TGGAATGACGCCGTCCTGATCAAGCGCATTCTCGATATCGGCGCCCGCGGCCTGCTGGTCCCCTTCGTGCAGAATGCGGA
CGAAGCGCGCCGCGCGGTCGCCGCTACCCGCTATCCGCCCCTCGGAATCCGCGGCGTCTCGGTCGCCCCGCGCGCGAATT
TGTACGGCCGCGTGCCCAACTACCACGCCGCCGCGCATGACGAAATCTGTGTGCTGGTGCAGGTGGAGACCGCCGCCGCT
GTCAACGAAATCGAAGCCATCGCCGCCGTCGAAGGCGTGGACGGAATCTTCATCGGCCCCAGCGACCTGGCCGCCGACCT
CGGGCACCTCGGCAATCCCAATCACCCCGATGTGCAGTCCGCCATCGCGTCCGCCGGCCGCCGCATCCGCGCCGCCGGCA
AAGCCTCCGGCATCCTCACCGGCGACGCCGAGTCCGCCGCCCGCTTCTTCCAACTCGGCTTCACCTTCGTCGCCGCAGGC
AGCGACGTCGGCACCCTCGCCCGCGGCACCTCCACCCTCGCCGCCCAGTGCCGCGAGAAGTTAGTGGGATCGGCCTCCCG
GCCGTCTCTTACCGCCCAAGAAGACACCTCATCGGAGCACCGACCGTCAGGGAGCGGTCGCTAA

Upstream 100 bases:

>100_bases
CAGATCTACGCCGCTATCTTGAAGGGCGATCCCACCGCCGCATCGCGCCACATGCGCACGCATCTCACCCAAAGCCGCGA
TCGTTTTCGAAGAGCCCAAG

Downstream 100 bases:

>100_bases
TTTGCCGGTTGATTACCGACCGCTTCCTCACGGTCGCGGCTTCGTCGGCGACGCCTACCTCACCAGAGACGGCCGCTTAT
TGTCGAACTTCCAGCCGGGG

Product: 2-dehydro-3-deoxyglucarate aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSQNNAALRPNQFKRALVEGRPQIGLWSSLCSNIAAEIICNSGFDWIVVDTEHAPNEVPGLVAQLQAMVNGTAEPVVRCA
WNDAVLIKRILDIGARGLLVPFVQNADEARRAVAATRYPPLGIRGVSVAPRANLYGRVPNYHAAAHDEICVLVQVETAAA
VNEIEAIAAVEGVDGIFIGPSDLAADLGHLGNPNHPDVQSAIASAGRRIRAAGKASGILTGDAESAARFFQLGFTFVAAG
SDVGTLARGTSTLAAQCREKLVGSASRPSLTAQEDTSSEHRPSGSGR

Sequences:

>Translated_287_residues
MSQNNAALRPNQFKRALVEGRPQIGLWSSLCSNIAAEIICNSGFDWIVVDTEHAPNEVPGLVAQLQAMVNGTAEPVVRCA
WNDAVLIKRILDIGARGLLVPFVQNADEARRAVAATRYPPLGIRGVSVAPRANLYGRVPNYHAAAHDEICVLVQVETAAA
VNEIEAIAAVEGVDGIFIGPSDLAADLGHLGNPNHPDVQSAIASAGRRIRAAGKASGILTGDAESAARFFQLGFTFVAAG
SDVGTLARGTSTLAAQCREKLVGSASRPSLTAQEDTSSEHRPSGSGR
>Mature_286_residues
SQNNAALRPNQFKRALVEGRPQIGLWSSLCSNIAAEIICNSGFDWIVVDTEHAPNEVPGLVAQLQAMVNGTAEPVVRCAW
NDAVLIKRILDIGARGLLVPFVQNADEARRAVAATRYPPLGIRGVSVAPRANLYGRVPNYHAAAHDEICVLVQVETAAAV
NEIEAIAAVEGVDGIFIGPSDLAADLGHLGNPNHPDVQSAIASAGRRIRAAGKASGILTGDAESAARFFQLGFTFVAAGS
DVGTLARGTSTLAAQCREKLVGSASRPSLTAQEDTSSEHRPSGSGR

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=255, Percent_Identity=50.5882352941176, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1788578, Length=238, Percent_Identity=49.1596638655462, Blast_Score=222, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 30032; Mature: 29901

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNNAALRPNQFKRALVEGRPQIGLWSSLCSNIAAEIICNSGFDWIVVDTEHAPNEVPG
CCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHH
LVAQLQAMVNGTAEPVVRCAWNDAVLIKRILDIGARGLLVPFVQNADEARRAVAATRYPP
HHHHHHHHHCCCCCHHEEECCCCHHHHHHHHHHCCCCEEHHHHCCHHHHHHHHHHCCCCC
LGIRGVSVAPRANLYGRVPNYHAAAHDEICVLVQVETAAAVNEIEAIAAVEGVDGIFIGP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCEEEECC
SDLAADLGHLGNPNHPDVQSAIASAGRRIRAAGKASGILTGDAESAARFFQLGFTFVAAG
HHHHHHHHHCCCCCCCHHHHHHHHCCHHEECCCCCCCEEECCHHHHHHHHHHHHHEEECC
SDVGTLARGTSTLAAQCREKLVGSASRPSLTAQEDTSSEHRPSGSGR
CCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQNNAALRPNQFKRALVEGRPQIGLWSSLCSNIAAEIICNSGFDWIVVDTEHAPNEVPG
CCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHH
LVAQLQAMVNGTAEPVVRCAWNDAVLIKRILDIGARGLLVPFVQNADEARRAVAATRYPP
HHHHHHHHHCCCCCHHEEECCCCHHHHHHHHHHCCCCEEHHHHCCHHHHHHHHHHCCCCC
LGIRGVSVAPRANLYGRVPNYHAAAHDEICVLVQVETAAAVNEIEAIAAVEGVDGIFIGP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCEEEECC
SDLAADLGHLGNPNHPDVQSAIASAGRRIRAAGKASGILTGDAESAARFFQLGFTFVAAG
HHHHHHHHHCCCCCCCHHHHHHHHCCHHEECCCCCCCEEECCHHHHHHHHHHHHHEEECC
SDVGTLARGTSTLAAQCREKLVGSASRPSLTAQEDTSSEHRPSGSGR
CCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA