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The map label for this gene is lutR [H]

Identifier: 116623075

GI number: 116623075

Start: 5046124

End: 5046852

Strand: Direct

Name: lutR [H]

Synonym: Acid_3979

Alternate gene names: 116623075

Gene position: 5046124-5046852 (Clockwise)

Preceding gene: 116623074

Following gene: 116623076

Centisome position: 50.64

GC content: 65.29

Gene sequence:

>729_bases
GTGAGTTCGCAGGCGGAGACCATCCGCCGCAAGCCGCGCCGCCTGGCCACGGAGCTGGTGGATTCGCTCTCCGAACAGAT
CCGCGGCCGGGCCCTCAAACCCGGCGATAAGCTGCCCACCGAGTCGGCCATTATGCAGAACTTTGGCGTCAGCCGCACCG
TGGTGCGCGAAGCCATCTCCGCCCTGCAGGCCGCCGGCTTCGTGGAGACCCGCCACGGCATCGGCACTTTCGTGCTGGAT
TCGCCCAGCGGCTTTGAGTTCCGAGTCGAACCGCGCAGCATCCCCACCGTCCGCGATATTCTCGCCATGCTGGAGCTCCG
CATCAGCCTGGAATCCGAGGCGGCCGCGCTCGCCGCCGAGCGCCGCACCGAAGCCCAACTCGCCGAGCTGCGCCGCGTGC
TTGACGCCTACCAAGTGGCACTGCACAGCGGCGGCGATACCGCCGAGCCCGATTTTCAATTCCACCTGCGCGTGGCCGAA
GCCACCGGCAACCGTTATTTCCCCGAGGTGCTCGCCCGCTTCGGCACCACCACCATTCCGCGCACCCGCGTCAGCCTTCT
GCAATCGCCCGGCGGCCAGACGGCCTTTCTTAGCACTTTGGGAAAAGAACACGAACAGATCTACGCCGCTATCTTGAAGG
GCGATCCCACCGCCGCATCGCGCCACATGCGCACGCATCTCACCCAAAGCCGCGATCGTTTTCGAAGAGCCCAAGATGTC
GCAAAATAA

Upstream 100 bases:

>100_bases
ACTACGAAGCTCCCATGTTCGCCTGTGCCGTCTTCCTCGTCCTGGGCGTGCTCTCCTACATCTTCATGGTCAAAGAGCGC
TACGCGCCGGAGATGGCGTC

Downstream 100 bases:

>100_bases
TGCCGCCCTGCGGCCCAACCAATTCAAGCGCGCCCTTGTCGAAGGCCGCCCTCAGATCGGCCTGTGGAGTTCCCTGTGCA
GCAACATCGCCGCCGAGATC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSSQAETIRRKPRRLATELVDSLSEQIRGRALKPGDKLPTESAIMQNFGVSRTVVREAISALQAAGFVETRHGIGTFVLD
SPSGFEFRVEPRSIPTVRDILAMLELRISLESEAAALAAERRTEAQLAELRRVLDAYQVALHSGGDTAEPDFQFHLRVAE
ATGNRYFPEVLARFGTTTIPRTRVSLLQSPGGQTAFLSTLGKEHEQIYAAILKGDPTAASRHMRTHLTQSRDRFRRAQDV
AK

Sequences:

>Translated_242_residues
MSSQAETIRRKPRRLATELVDSLSEQIRGRALKPGDKLPTESAIMQNFGVSRTVVREAISALQAAGFVETRHGIGTFVLD
SPSGFEFRVEPRSIPTVRDILAMLELRISLESEAAALAAERRTEAQLAELRRVLDAYQVALHSGGDTAEPDFQFHLRVAE
ATGNRYFPEVLARFGTTTIPRTRVSLLQSPGGQTAFLSTLGKEHEQIYAAILKGDPTAASRHMRTHLTQSRDRFRRAQDV
AK
>Mature_241_residues
SSQAETIRRKPRRLATELVDSLSEQIRGRALKPGDKLPTESAIMQNFGVSRTVVREAISALQAAGFVETRHGIGTFVLDS
PSGFEFRVEPRSIPTVRDILAMLELRISLESEAAALAAERRTEAQLAELRRVLDAYQVALHSGGDTAEPDFQFHLRVAEA
TGNRYFPEVLARFGTTTIPRTRVSLLQSPGGQTAFLSTLGKEHEQIYAAILKGDPTAASRHMRTHLTQSRDRFRRAQDVA
K

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=214, Percent_Identity=32.2429906542056, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082208, Length=223, Percent_Identity=30.4932735426009, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790032, Length=233, Percent_Identity=28.3261802575107, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789353, Length=234, Percent_Identity=26.0683760683761, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786303, Length=208, Percent_Identity=31.7307692307692, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789621, Length=238, Percent_Identity=26.890756302521, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI48994955, Length=225, Percent_Identity=26.6666666666667, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26809; Mature: 26678

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQAETIRRKPRRLATELVDSLSEQIRGRALKPGDKLPTESAIMQNFGVSRTVVREAIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
ALQAAGFVETRHGIGTFVLDSPSGFEFRVEPRSIPTVRDILAMLELRISLESEAAALAAE
HHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RRTEAQLAELRRVLDAYQVALHSGGDTAEPDFQFHLRVAEATGNRYFPEVLARFGTTTIP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCC
RTRVSLLQSPGGQTAFLSTLGKEHEQIYAAILKGDPTAASRHMRTHLTQSRDRFRRAQDV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
AK
CC
>Mature Secondary Structure 
SSQAETIRRKPRRLATELVDSLSEQIRGRALKPGDKLPTESAIMQNFGVSRTVVREAIS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
ALQAAGFVETRHGIGTFVLDSPSGFEFRVEPRSIPTVRDILAMLELRISLESEAAALAAE
HHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RRTEAQLAELRRVLDAYQVALHSGGDTAEPDFQFHLRVAEATGNRYFPEVLARFGTTTIP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCC
RTRVSLLQSPGGQTAFLSTLGKEHEQIYAAILKGDPTAASRHMRTHLTQSRDRFRRAQDV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
AK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]