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The map label for this gene is adaA [H]

Identifier: 116622729

GI number: 116622729

Start: 4594347

End: 4595150

Strand: Direct

Name: adaA [H]

Synonym: Acid_3628

Alternate gene names: 116622729

Gene position: 4594347-4595150 (Clockwise)

Preceding gene: 116622728

Following gene: 116622732

Centisome position: 46.1

GC content: 64.3

Gene sequence:

>804_bases
ATGAACCGCGATCCGGGTTTGTACGGAATGACCGTCAAGTCACGATCGCTCGGCGATTTCATGATCAGTGAACGCGCCTA
TCCCGCGCGCTACACGACTCCTGCGCATGCCCACGAGCGCCCGCTCTACTGCGTGGTGCTGGACGGTTCTTACGAAGAAA
TCCACGAGGGCCGCCGCCACGAATGCACCTCTTCCACCATGCTCTTTCACGCCGCCCACGAGGAGCACCTGGAACGCTTC
GGCGGCGAGGGCGGGCGCTCCCTAATTGTCGAGATCGAGCCTTCGTGGTACGACCGCGTCTGCGAACTGGCGCCCGGCGG
CGTTCACACCGCGGCCTGCGACGGCGGCTCTCTGCGCGTATCCGGCGCCAAGTTGTATCGCGAATTCCTCAGCGGCGACG
AGGCTTCGCGCCTGATCATAGAAGGTCTCCTGCTCGAAATCACGGGCGAATTTCTGCGCATCCGCCGGACTCCGGAGTCG
GCACCTCCGCGCTGGCTCGAAGATGTTCGCGAGTACGTGCGGGCCAATTTCGCCGGCCGCATTACGCTTGGCTCGATCGG
CAAGGCCGCTAACGTCCATCCGGTCCATGTGGCCCAGACCTTCCGCCGATTCCATCACTGCACCGTCGGCGATTACCTGC
GCAGCCTGCGCATCGAGTTTGCCTGCCGTGAACTGGCCGCCTCCGAAACTCCCGTTGCCGATATCGCGGCCGCCGCCGGT
TTCACTACGCCCAGCCACTTCCACCGCATCTTCAAGCGCGCCGTCGGGGTCTCCCCCACCCAATACCGGACCCACGCACG
GTAG

Upstream 100 bases:

>100_bases
GAGCAGTGTAATCCTCAGATGCCGCGGTCAGATCACGACCCTCACATTACGGCAGCATTCTTCTTGCAGTAGAATTGAAC
GAATGCTGGGAGCCGGCGGG

Downstream 100 bases:

>100_bases
CGAATTACAAATACGTCGGCATCACTTGCAGCTCTGGCACGGCCACGCGCGCCGGCATTTTAGCCACCGCCAGGATCGCT
TCCGCCACATCCGCCGGCTG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNRDPGLYGMTVKSRSLGDFMISERAYPARYTTPAHAHERPLYCVVLDGSYEEIHEGRRHECTSSTMLFHAAHEEHLERF
GGEGGRSLIVEIEPSWYDRVCELAPGGVHTAACDGGSLRVSGAKLYREFLSGDEASRLIIEGLLLEITGEFLRIRRTPES
APPRWLEDVREYVRANFAGRITLGSIGKAANVHPVHVAQTFRRFHHCTVGDYLRSLRIEFACRELAASETPVADIAAAAG
FTTPSHFHRIFKRAVGVSPTQYRTHAR

Sequences:

>Translated_267_residues
MNRDPGLYGMTVKSRSLGDFMISERAYPARYTTPAHAHERPLYCVVLDGSYEEIHEGRRHECTSSTMLFHAAHEEHLERF
GGEGGRSLIVEIEPSWYDRVCELAPGGVHTAACDGGSLRVSGAKLYREFLSGDEASRLIIEGLLLEITGEFLRIRRTPES
APPRWLEDVREYVRANFAGRITLGSIGKAANVHPVHVAQTFRRFHHCTVGDYLRSLRIEFACRELAASETPVADIAAAAG
FTTPSHFHRIFKRAVGVSPTQYRTHAR
>Mature_267_residues
MNRDPGLYGMTVKSRSLGDFMISERAYPARYTTPAHAHERPLYCVVLDGSYEEIHEGRRHECTSSTMLFHAAHEEHLERF
GGEGGRSLIVEIEPSWYDRVCELAPGGVHTAACDGGSLRVSGAKLYREFLSGDEASRLIIEGLLLEITGEFLRIRRTPES
APPRWLEDVREYVRANFAGRITLGSIGKAANVHPVHVAQTFRRFHHCTVGDYLRSLRIEFACRELAASETPVADIAAAAG
FTTPSHFHRIFKRAVGVSPTQYRTHAR

Specific function: Methylphosphotriester-DNA alkyltransferase is one of two genes required for the adaptive response of bacteria to alkylating agents. It accepts a methyl group from methylphospho-triesters and then acts as a transcriptional activator of the ada operon [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 2500 (L-broth) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR016220 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 29877; Mature: 29877

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRDPGLYGMTVKSRSLGDFMISERAYPARYTTPAHAHERPLYCVVLDGSYEEIHEGRRH
CCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCC
ECTSSTMLFHAAHEEHLERFGGEGGRSLIVEIEPSWYDRVCELAPGGVHTAACDGGSLRV
CCCCHHHEEEHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCCCCCEEEECCCCCEEE
SGAKLYREFLSGDEASRLIIEGLLLEITGEFLRIRRTPESAPPRWLEDVREYVRANFAGR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCE
ITLGSIGKAANVHPVHVAQTFRRFHHCTVGDYLRSLRIEFACRELAASETPVADIAAAAG
EEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
FTTPSHFHRIFKRAVGVSPTQYRTHAR
CCCHHHHHHHHHHHHCCCCHHHCCCCC
>Mature Secondary Structure
MNRDPGLYGMTVKSRSLGDFMISERAYPARYTTPAHAHERPLYCVVLDGSYEEIHEGRRH
CCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCC
ECTSSTMLFHAAHEEHLERFGGEGGRSLIVEIEPSWYDRVCELAPGGVHTAACDGGSLRV
CCCCHHHEEEHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCCCCCEEEECCCCCEEE
SGAKLYREFLSGDEASRLIIEGLLLEITGEFLRIRRTPESAPPRWLEDVREYVRANFAGR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCE
ITLGSIGKAANVHPVHVAQTFRRFHHCTVGDYLRSLRIEFACRELAASETPVADIAAAAG
EEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
FTTPSHFHRIFKRAVGVSPTQYRTHAR
CCCHHHHHHHHHHHHCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]