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The map label for this gene is pitA [H]

Identifier: 116622602

GI number: 116622602

Start: 4428853

End: 4430448

Strand: Direct

Name: pitA [H]

Synonym: Acid_3500

Alternate gene names: 116622602

Gene position: 4428853-4430448 (Clockwise)

Preceding gene: 116622601

Following gene: 116622603

Centisome position: 44.44

GC content: 62.97

Gene sequence:

>1596_bases
ATGGCAGATGCTCGCGTATCGGTGCTCGAGGCCAAGCTCGCGCGTAAGCCGGGGCGCCTCTCTTTGATCATTTTCGCCGC
CGTTCTGCTCGGCGGCATCGCCTACACCGGAGGCAGCCTGCTTTCGGACCTCTCCCAGCTGCGCAACACTCCCGGCTTCG
AATTTCTGCTCCTGGGGCTGACGCTCGCGATTGCGCTCGGCTTCGAATTCGTCAACGGTTTCCACGACACGGCCAACGCC
GTCGCGACCGTGATTTACACGCACTCCCTGGAACCGCACCTTGCCGTGGTCTGGTCCGGCCTGTGGAACTTCATCGGCGT
ACTGACGTCGAGCGGTGCCGTGGCCTTTGGCATCGTGTCCCTGCTGCCGGTCGAATTGATTCTCAATGCGGGAACCCACG
CCGGCTTTGCCATGATTTTCGCGCTCCTTTTCTCAGCGATCTTGTGGAATCTGGGCACCTGGTATTTCGGCTTGCCGAAC
TCCAGCTCCCATACCCTGATCGGGTCCATCCTCGGCGTCGGCCTGATGAACCAGTGGATGGCGCCGCTGCACTCCGGCGT
CAGCGGTGTGGATTGGACCCAGGCCATCGGCGTCGGCAAAAGCCTGTTTTTCTCTCCCCTCATCGGCTTCGTCTGCTCCG
CTCTTCTCTTTCTGCTGATTAAGCGCTGCGTGCGCGACCCTCGCCTTTACTCTGAACCGGTGGGCAAGGCGCCGCCGCCG
TTCTGGATTCGATCGATGCTGGTCTTGACCTGCACCGGGGTCAGCTACGCGCATGGCTCCAACGATGGACAGAAAGGCAT
GGGGTTGATCATGCTGATCCTGGTGGGAACGGTGCCCGCGGCCTATGCCTTGAATCATGCCGTCAGCGGTCCCGAAGTTC
AGGCGTTCCTCGCTATCTCCGAGCGCACCGGCCGCATACTCGAGCACTACAGGCCTGCCGGCGTCGAGGCTCCTGCCAAA
ATCAGGCCGGTCCTGGAGCGGCTCGTCAGCCAATCGTCCATGGAGCCGCAAACCGTGCCCGCATTGACGGCGGTGATCAG
TCGCATTGCGGCCGACGTGGCCCCTTACCGCGCACTGGCCAGCGTACCGATCGAGCTTTCCGGAAACATCCGCAACGATA
TGTACCTGGCCGACGAGGCCCTCGGCGTTGTCGAAGCCAAACACCTGTTGCCGCTCTCCGCGGCCGACGCCGAGGCCATC
CGGGACTACCGCCGCACGATCGACCGCGCCGCCAAATTTATACCGACTTGGGTGAAGGTCGCCGTGGCCCTGGCGCTCGG
ACTCGGCACCATGGTTGGCTGGAAGCGCATCGTAGTCACAGTCGGCGAGCGAATCGGCAAGACTCACCTGACCTACGCGC
AAGGAGCAGCCTCGGAAGTGATCGCGATGGCGACCATCCTGGCCGCCGATCGCGCTGGGATGCCCGTAAGCACGACTCAT
GTCTTGACCAGCGGCGTCGCCGGCACCATGGCAGCCAATCACTCCGGTCTGCAATGGAGCACGCTGCGCGCCCTGGCGGC
CGCCTGGCTGATGACGCTGCCTGCCTCGGTAGCATTGGCAGCCAGTCTCTTCTGGGTATTCCGTCACATCATGTAG

Upstream 100 bases:

>100_bases
GCTCCAGCCAAAAGACATCCCCGGGCAAAGCACCAGCAAAAAGCGGCCGAAATCGAAGTAACGGTTTTGTAACTCGCGCT
TGCTAGTCTTGGAACCAGCC

Downstream 100 bases:

>100_bases
TTTCACGCCCGCCATCGGCGAAAAAACCGCCGCGTCTCGCCCGATGTTTACAACCTTTTTACGGTTCTCTTACCTGATCG
CTGTCAATTCCGATCGCAAA

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MADARVSVLEAKLARKPGRLSLIIFAAVLLGGIAYTGGSLLSDLSQLRNTPGFEFLLLGLTLAIALGFEFVNGFHDTANA
VATVIYTHSLEPHLAVVWSGLWNFIGVLTSSGAVAFGIVSLLPVELILNAGTHAGFAMIFALLFSAILWNLGTWYFGLPN
SSSHTLIGSILGVGLMNQWMAPLHSGVSGVDWTQAIGVGKSLFFSPLIGFVCSALLFLLIKRCVRDPRLYSEPVGKAPPP
FWIRSMLVLTCTGVSYAHGSNDGQKGMGLIMLILVGTVPAAYALNHAVSGPEVQAFLAISERTGRILEHYRPAGVEAPAK
IRPVLERLVSQSSMEPQTVPALTAVISRIAADVAPYRALASVPIELSGNIRNDMYLADEALGVVEAKHLLPLSAADAEAI
RDYRRTIDRAAKFIPTWVKVAVALALGLGTMVGWKRIVVTVGERIGKTHLTYAQGAASEVIAMATILAADRAGMPVSTTH
VLTSGVAGTMAANHSGLQWSTLRALAAAWLMTLPASVALAASLFWVFRHIM

Sequences:

>Translated_531_residues
MADARVSVLEAKLARKPGRLSLIIFAAVLLGGIAYTGGSLLSDLSQLRNTPGFEFLLLGLTLAIALGFEFVNGFHDTANA
VATVIYTHSLEPHLAVVWSGLWNFIGVLTSSGAVAFGIVSLLPVELILNAGTHAGFAMIFALLFSAILWNLGTWYFGLPN
SSSHTLIGSILGVGLMNQWMAPLHSGVSGVDWTQAIGVGKSLFFSPLIGFVCSALLFLLIKRCVRDPRLYSEPVGKAPPP
FWIRSMLVLTCTGVSYAHGSNDGQKGMGLIMLILVGTVPAAYALNHAVSGPEVQAFLAISERTGRILEHYRPAGVEAPAK
IRPVLERLVSQSSMEPQTVPALTAVISRIAADVAPYRALASVPIELSGNIRNDMYLADEALGVVEAKHLLPLSAADAEAI
RDYRRTIDRAAKFIPTWVKVAVALALGLGTMVGWKRIVVTVGERIGKTHLTYAQGAASEVIAMATILAADRAGMPVSTTH
VLTSGVAGTMAANHSGLQWSTLRALAAAWLMTLPASVALAASLFWVFRHIM
>Mature_530_residues
ADARVSVLEAKLARKPGRLSLIIFAAVLLGGIAYTGGSLLSDLSQLRNTPGFEFLLLGLTLAIALGFEFVNGFHDTANAV
ATVIYTHSLEPHLAVVWSGLWNFIGVLTSSGAVAFGIVSLLPVELILNAGTHAGFAMIFALLFSAILWNLGTWYFGLPNS
SSHTLIGSILGVGLMNQWMAPLHSGVSGVDWTQAIGVGKSLFFSPLIGFVCSALLFLLIKRCVRDPRLYSEPVGKAPPPF
WIRSMLVLTCTGVSYAHGSNDGQKGMGLIMLILVGTVPAAYALNHAVSGPEVQAFLAISERTGRILEHYRPAGVEAPAKI
RPVLERLVSQSSMEPQTVPALTAVISRIAADVAPYRALASVPIELSGNIRNDMYLADEALGVVEAKHLLPLSAADAEAIR
DYRRTIDRAAKFIPTWVKVAVALALGLGTMVGWKRIVVTVGERIGKTHLTYAQGAASEVIAMATILAADRAGMPVSTTHV
LTSGVAGTMAANHSGLQWSTLRALAAAWLMTLPASVALAASLFWVFRHIM

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=478, Percent_Identity=40.3765690376569, Blast_Score=342, Evalue=4e-95,
Organism=Escherichia coli, GI1789360, Length=489, Percent_Identity=39.8773006134969, Blast_Score=334, Evalue=8e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 56407; Mature: 56276

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADARVSVLEAKLARKPGRLSLIIFAAVLLGGIAYTGGSLLSDLSQLRNTPGFEFLLLGL
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHH
TLAIALGFEFVNGFHDTANAVATVIYTHSLEPHLAVVWSGLWNFIGVLTSSGAVAFGIVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LLPVELILNAGTHAGFAMIFALLFSAILWNLGTWYFGLPNSSSHTLIGSILGVGLMNQWM
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
APLHSGVSGVDWTQAIGVGKSLFFSPLIGFVCSALLFLLIKRCVRDPRLYSEPVGKAPPP
HHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCC
FWIRSMLVLTCTGVSYAHGSNDGQKGMGLIMLILVGTVPAAYALNHAVSGPEVQAFLAIS
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEH
ERTGRILEHYRPAGVEAPAKIRPVLERLVSQSSMEPQTVPALTAVISRIAADVAPYRALA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SVPIELSGNIRNDMYLADEALGVVEAKHLLPLSAADAEAIRDYRRTIDRAAKFIPTWVKV
HCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AVALALGLGTMVGWKRIVVTVGERIGKTHLTYAQGAASEVIAMATILAADRAGMPVSTTH
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHH
VLTSGVAGTMAANHSGLQWSTLRALAAAWLMTLPASVALAASLFWVFRHIM
HHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ADARVSVLEAKLARKPGRLSLIIFAAVLLGGIAYTGGSLLSDLSQLRNTPGFEFLLLGL
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHH
TLAIALGFEFVNGFHDTANAVATVIYTHSLEPHLAVVWSGLWNFIGVLTSSGAVAFGIVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LLPVELILNAGTHAGFAMIFALLFSAILWNLGTWYFGLPNSSSHTLIGSILGVGLMNQWM
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
APLHSGVSGVDWTQAIGVGKSLFFSPLIGFVCSALLFLLIKRCVRDPRLYSEPVGKAPPP
HHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCC
FWIRSMLVLTCTGVSYAHGSNDGQKGMGLIMLILVGTVPAAYALNHAVSGPEVQAFLAIS
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEH
ERTGRILEHYRPAGVEAPAKIRPVLERLVSQSSMEPQTVPALTAVISRIAADVAPYRALA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SVPIELSGNIRNDMYLADEALGVVEAKHLLPLSAADAEAIRDYRRTIDRAAKFIPTWVKV
HCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AVALALGLGTMVGWKRIVVTVGERIGKTHLTYAQGAASEVIAMATILAADRAGMPVSTTH
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHH
VLTSGVAGTMAANHSGLQWSTLRALAAAWLMTLPASVALAASLFWVFRHIM
HHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]