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The map label for this gene is ntrX [H]

Identifier: 116622515

GI number: 116622515

Start: 4319365

End: 4320726

Strand: Reverse

Name: ntrX [H]

Synonym: Acid_3413

Alternate gene names: 116622515

Gene position: 4320726-4319365 (Counterclockwise)

Preceding gene: 116622516

Following gene: 116622514

Centisome position: 43.36

GC content: 62.48

Gene sequence:

>1362_bases
ATGAAGGCTCACCGGGTCCTGGTGGTGGATGACGAGCCCGCCATCCGCCAGTCCTTGAGCGGCGTCCTCGAGGACGAAGG
TTACGCCGTGGAATCGGCCGAAAGCGGCGAGGCATGTCTGGAGGCATTGCCCGCCGGCAATTTCGAACTCGTACTGCTCG
ATATCTGGCTCCCCGGCATGGACGGCATGGAAGTCCTCTCGCGCATTCAGGAGATCCCGTTCGGCGAACGCCCCGTGGTC
GTGGTGATCAGCGGGCACGGCTCCATCGAAGCCGCCGTGAAAGCCACCAAACTGGGCGCATTCGACTTCCTCGAAAAACC
GCTCTCCATCGAAAAGGTCTCGGTAGTCACCAAGAACGCCCTCAACCACCGCCGCCTCGAACTCGAGAACAGCCGCCTCA
AAGAGGATACCGGCTCGCGCTGGCGCATCATCGGCGAAAGCGTGCCCATGAAGGCGCTCCGCCAGCAGCTCACACTCATG
GCCGGCACCAATGGCCGCGTCCTGATCTATGGCGAAAGCGGCACCGGCAAGGAACTGGTCGCCCACGCCCTGCACGCGCT
CAGCCCGCGCGCCGCCGAGCCCTTCGTCGAAGTCAACTGCGCCGCCATTCCCGAAGAGCTGATCGAAAGCGAAATGTTCG
GTCACATCAGGGGCAGCTTCACCAGCGCCCAGGAGGACAAGGTCGGCAAATTCCAGAAGGCCGATGGCGGCACGCTCTTC
CTGGACGAAGTCGGCGACATGAGCCTGCGCACCCAGAGCAAAGTGCTCCGCGCCCTCGAAGAACAGCGCTTCGAACCCGT
CGGAGCCCCGCAATCCGTACTCGTGGACGTGCGCGTGGTCGCGGCCACCAACAAGAACCTGGAAGAGGAGATCGAACGCG
GCAACTTCCGCGAAGACCTCTTCTACCGGCTCAACGTCATCCCGTTTTTCGTCCCGCCCCTGCGCGACCGGCAGGAGGAT
ATCCCGCTGCTCGCCGATCACTTCCTGCGCGAGTTCACCACCGCCTACGGCCGCAAACCGAAAGAGCTCACGCCGGAGGC
TTACCGCGTCCTCATCGAATATCACTGGCCGGGCAATGTCCGCGAATTGAAAAACCTGATCGAACGCATCGTAATTTTGA
ACCCGCAAGTCCGCGTCGATGCCCGTCACATCCCCTTGCAGACCGCCCGGCGCCAGCCCGACCGGCCACTCGATCGCTTC
GGCAGCTTGCAGGAAGTGCGCGAAGCGGCCGAGCGCGAGTACATCCTCAAGAAGCTCGAGGAGACCAATGGCAATGTCAC
CCGGACCGCGGAATTGCTGGGCCTGGAGAGAAGCAATCTCTATCGCAAGATGAAGACGCTCGGCATCGGACCCAAGGAAT
AA

Upstream 100 bases:

>100_bases
TGGAGGACAACCAGCCCGTGGGAGCCCGTTTTACGGTGGAGCTTCCCGCCATCGTGGAAAACGAGGCGGCCGAGCCCCCC
CTGCGCGCGGCCACCAGAGT

Downstream 100 bases:

>100_bases
ATTTCCGGTCAACACTATACTATTGACTCTGCCAAAGTAAACATCCTACTATTGACTCATGGGAAGTAAACAAAAACTTG
GTCGCGCTTTAGGCCCCTAC

Product: two component sigma54-specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKAHRVLVVDDEPAIRQSLSGVLEDEGYAVESAESGEACLEALPAGNFELVLLDIWLPGMDGMEVLSRIQEIPFGERPVV
VVISGHGSIEAAVKATKLGAFDFLEKPLSIEKVSVVTKNALNHRRLELENSRLKEDTGSRWRIIGESVPMKALRQQLTLM
AGTNGRVLIYGESGTGKELVAHALHALSPRAAEPFVEVNCAAIPEELIESEMFGHIRGSFTSAQEDKVGKFQKADGGTLF
LDEVGDMSLRTQSKVLRALEEQRFEPVGAPQSVLVDVRVVAATNKNLEEEIERGNFREDLFYRLNVIPFFVPPLRDRQED
IPLLADHFLREFTTAYGRKPKELTPEAYRVLIEYHWPGNVRELKNLIERIVILNPQVRVDARHIPLQTARRQPDRPLDRF
GSLQEVREAAEREYILKKLEETNGNVTRTAELLGLERSNLYRKMKTLGIGPKE

Sequences:

>Translated_453_residues
MKAHRVLVVDDEPAIRQSLSGVLEDEGYAVESAESGEACLEALPAGNFELVLLDIWLPGMDGMEVLSRIQEIPFGERPVV
VVISGHGSIEAAVKATKLGAFDFLEKPLSIEKVSVVTKNALNHRRLELENSRLKEDTGSRWRIIGESVPMKALRQQLTLM
AGTNGRVLIYGESGTGKELVAHALHALSPRAAEPFVEVNCAAIPEELIESEMFGHIRGSFTSAQEDKVGKFQKADGGTLF
LDEVGDMSLRTQSKVLRALEEQRFEPVGAPQSVLVDVRVVAATNKNLEEEIERGNFREDLFYRLNVIPFFVPPLRDRQED
IPLLADHFLREFTTAYGRKPKELTPEAYRVLIEYHWPGNVRELKNLIERIVILNPQVRVDARHIPLQTARRQPDRPLDRF
GSLQEVREAAEREYILKKLEETNGNVTRTAELLGLERSNLYRKMKTLGIGPKE
>Mature_453_residues
MKAHRVLVVDDEPAIRQSLSGVLEDEGYAVESAESGEACLEALPAGNFELVLLDIWLPGMDGMEVLSRIQEIPFGERPVV
VVISGHGSIEAAVKATKLGAFDFLEKPLSIEKVSVVTKNALNHRRLELENSRLKEDTGSRWRIIGESVPMKALRQQLTLM
AGTNGRVLIYGESGTGKELVAHALHALSPRAAEPFVEVNCAAIPEELIESEMFGHIRGSFTSAQEDKVGKFQKADGGTLF
LDEVGDMSLRTQSKVLRALEEQRFEPVGAPQSVLVDVRVVAATNKNLEEEIERGNFREDLFYRLNVIPFFVPPLRDRQED
IPLLADHFLREFTTAYGRKPKELTPEAYRVLIEYHWPGNVRELKNLIERIVILNPQVRVDARHIPLQTARRQPDRPLDRF
GSLQEVREAAEREYILKKLEETNGNVTRTAELLGLERSNLYRKMKTLGIGPKE

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=461, Percent_Identity=39.6963123644252, Blast_Score=348, Evalue=5e-97,
Organism=Escherichia coli, GI1790437, Length=443, Percent_Identity=42.6636568848758, Blast_Score=339, Evalue=3e-94,
Organism=Escherichia coli, GI1790299, Length=469, Percent_Identity=41.36460554371, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=40.3153153153153, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI87082117, Length=343, Percent_Identity=44.3148688046647, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1789087, Length=354, Percent_Identity=42.3728813559322, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1789233, Length=318, Percent_Identity=42.7672955974843, Blast_Score=247, Evalue=9e-67,
Organism=Escherichia coli, GI87082152, Length=318, Percent_Identity=42.7672955974843, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1786524, Length=323, Percent_Identity=38.6996904024768, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1787583, Length=329, Percent_Identity=38.2978723404255, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI87081872, Length=309, Percent_Identity=39.8058252427184, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI87081858, Length=285, Percent_Identity=30.8771929824561, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1789828, Length=222, Percent_Identity=34.2342342342342, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1790552, Length=109, Percent_Identity=30.2752293577982, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789149, Length=111, Percent_Identity=31.5315315315315, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789402, Length=209, Percent_Identity=25.8373205741627, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI87082012, Length=239, Percent_Identity=25.1046025104602, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50897; Mature: 50897

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAHRVLVVDDEPAIRQSLSGVLEDEGYAVESAESGEACLEALPAGNFELVLLDIWLPGM
CCCCEEEEECCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHCCCCCEEEEEEEEECCCC
DGMEVLSRIQEIPFGERPVVVVISGHGSIEAAVKATKLGAFDFLEKPLSIEKVSVVTKNA
CHHHHHHHHHHCCCCCCCEEEEEECCCCEEHHHHHHHCCHHHHHHCCCCHHHHHHHHHHH
LNHRRLELENSRLKEDTGSRWRIIGESVPMKALRQQLTLMAGTNGRVLIYGESGTGKELV
HCHHHEECCHHHHCCCCCCEEEEECCCCCHHHHHHHHHHEECCCCEEEEEECCCCCHHHH
AHALHALSPRAAEPFVEVNCAAIPEELIESEMFGHIRGSFTSAQEDKVGKFQKADGGTLF
HHHHHHCCCCCCCCCEEEEHHHCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEE
LDEVGDMSLRTQSKVLRALEEQRFEPVGAPQSVLVDVRVVAATNKNLEEEIERGNFREDL
EECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEEEEEECCCCHHHHHHCCCCHHHH
FYRLNVIPFFVPPLRDRQEDIPLLADHFLREFTTAYGRKPKELTPEAYRVLIEYHWPGNV
HHHEEEHEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEECCCCH
RELKNLIERIVILNPQVRVDARHIPLQTARRQPDRPLDRFGSLQEVREAAEREYILKKLE
HHHHHHHHHHHCCCCCEEECCCCCCHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHH
ETNGNVTRTAELLGLERSNLYRKMKTLGIGPKE
HCCCCEEEHHHHHCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKAHRVLVVDDEPAIRQSLSGVLEDEGYAVESAESGEACLEALPAGNFELVLLDIWLPGM
CCCCEEEEECCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHCCCCCEEEEEEEEECCCC
DGMEVLSRIQEIPFGERPVVVVISGHGSIEAAVKATKLGAFDFLEKPLSIEKVSVVTKNA
CHHHHHHHHHHCCCCCCCEEEEEECCCCEEHHHHHHHCCHHHHHHCCCCHHHHHHHHHHH
LNHRRLELENSRLKEDTGSRWRIIGESVPMKALRQQLTLMAGTNGRVLIYGESGTGKELV
HCHHHEECCHHHHCCCCCCEEEEECCCCCHHHHHHHHHHEECCCCEEEEEECCCCCHHHH
AHALHALSPRAAEPFVEVNCAAIPEELIESEMFGHIRGSFTSAQEDKVGKFQKADGGTLF
HHHHHHCCCCCCCCCEEEEHHHCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEE
LDEVGDMSLRTQSKVLRALEEQRFEPVGAPQSVLVDVRVVAATNKNLEEEIERGNFREDL
EECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEEEEEECCCCHHHHHHCCCCHHHH
FYRLNVIPFFVPPLRDRQEDIPLLADHFLREFTTAYGRKPKELTPEAYRVLIEYHWPGNV
HHHEEEHEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEECCCCH
RELKNLIERIVILNPQVRVDARHIPLQTARRQPDRPLDRFGSLQEVREAAEREYILKKLE
HHHHHHHHHHHCCCCCEEECCCCCCHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHH
ETNGNVTRTAELLGLERSNLYRKMKTLGIGPKE
HCCCCEEEHHHHHCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]