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The map label for this gene is oprM [H]

Identifier: 116622509

GI number: 116622509

Start: 4311073

End: 4312491

Strand: Reverse

Name: oprM [H]

Synonym: Acid_3407

Alternate gene names: 116622509

Gene position: 4312491-4311073 (Counterclockwise)

Preceding gene: 116622510

Following gene: 116622508

Centisome position: 43.27

GC content: 64.9

Gene sequence:

>1419_bases
GTGAGACTCGCGGTCGCGCTGCTCGCCCTTCTCGCCGTGGGCTGCTCGGTCGGTCCCAAATACCAGCGGCCAATGATCCA
GGCGCCCCCTGCCTACAAAGAGTCGCAAGGCTGGAAGACCGCGCAGCCCGCCGACACCGTGCTGCGCGGCAACTGGTGGA
CGCTCTTCGGAGATGCCGAACTCAACGCTCTCGAACAACAAATCGATCTCTCGAACCAGAATTTGAAAGCCGCCGAAGCG
CAGTTCCAACAGGCCCGCGCGCTGATTCGCTTCACCCAGTCTCAGAAGTACCCCACCGTAACGGTCGGCGCCGGCGTCAG
CGGCAACCGGAACTCGGCGACTTATGCGTACGCCAGTCCCAAGACCTCCAACACGTACGGCAATTTCGTCCTGCCCTTCG
ACGTCTCCTATGAAGTGGATGCATGGGGACGCGTGCGCCAGTCCATTGAAGCCGCCCGCACCAACGCGCAGGCGACCGAC
GCCGACCTCGAAACCCTGCGCCTCAGCTTCCATGCCGAGCTGGCCTTCGACTATTTCGAAATGCGCAGCGCCGATGCCGA
ACAGAAACTGCTCGACGATACCGTGGTCGCGTACCGCAAGGCTCTCGACTTGACCGAGAATCGCTTCGCGGGAGGTGTGG
CCGCGGGCGCGGAAGTCGCCCAGGCGCAGACTCAGCTGGAGTCCACCAAGACCCTGGCCACCGATGTCGCCATTCGCCGT
GCGCAATTTGAACACGCCATCGCGGTGCTGGCCGGCAAGAATCCTTCGGCGCTCACGATCGCACCCAAGCCGCTGGCCGC
CGAGCCTCCCGCGATCCCGGTCGGAGTGCCTTCCCAACTTCTGGAACGCCGGCCCGATATCGCCGCCAACGAGCGCCGCG
TCGCCGAGGCCAATGCCGAAGTCGGCATCGCGCGCACCGCCTTCTTCCCGCAGCTTCTTCTCGGCGCCACCGCCGGACTG
GAGGGCCAATCCATCACCGACTGGATCAATTGGCCGAGCCGCTTCTGGTCGATCGGGCCGAGCGCTATTCAGACGATCTT
CGATGGCGGCCGCCGCCGCGCCACTCAGAAGGCCGCGGAGTTCAACTACGACGCTACCGTCGCCACCTATCGCCAGACCG
CGCTCGACGCGTTCCAACAGGTAGAAGACAACCTGATCGCGCTGCGCGTGCTCGAGCAGGAAACGCAGTCGCAGCGCGCC
GCCGTGATCGCCGCCGAACGCTCGGTCGAACTTTCGACCAACCGTTACACCGGAGGCTTGGTGACGTACCTCGAAGTGGT
TACGGCGCAGAGCGCCGCGCTCAATAACGAACGCGCTGTCGTGGATATCCTGCGCCGCCGCATGGACGCCAGCGTCCTGC
TCATCAAAGCCCTCGGCGGCGGCTGGGACACCTCGCGCCTCCCAAAGCTGGCGAGCTAG

Upstream 100 bases:

>100_bases
AGATCATCTCCGGCTTGAATCCGCGCGATGAGGTGATCGCCAATCCCGCCGACTCGCTCACCTCCGGCGAGCCGGTCCGC
ATCGCCGCTGGAGGTGCCCG

Downstream 100 bases:

>100_bases
GAACGGACGCTCCCTCACGGTGGCGGCTCTGATAGGTCACGCTTATGCGAACCGCGACCGTCAGGGAGTAAGATAGCTGG
ATGAATCCAACCCGGCGTGA

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MRLAVALLALLAVGCSVGPKYQRPMIQAPPAYKESQGWKTAQPADTVLRGNWWTLFGDAELNALEQQIDLSNQNLKAAEA
QFQQARALIRFTQSQKYPTVTVGAGVSGNRNSATYAYASPKTSNTYGNFVLPFDVSYEVDAWGRVRQSIEAARTNAQATD
ADLETLRLSFHAELAFDYFEMRSADAEQKLLDDTVVAYRKALDLTENRFAGGVAAGAEVAQAQTQLESTKTLATDVAIRR
AQFEHAIAVLAGKNPSALTIAPKPLAAEPPAIPVGVPSQLLERRPDIAANERRVAEANAEVGIARTAFFPQLLLGATAGL
EGQSITDWINWPSRFWSIGPSAIQTIFDGGRRRATQKAAEFNYDATVATYRQTALDAFQQVEDNLIALRVLEQETQSQRA
AVIAAERSVELSTNRYTGGLVTYLEVVTAQSAALNNERAVVDILRRRMDASVLLIKALGGGWDTSRLPKLAS

Sequences:

>Translated_472_residues
MRLAVALLALLAVGCSVGPKYQRPMIQAPPAYKESQGWKTAQPADTVLRGNWWTLFGDAELNALEQQIDLSNQNLKAAEA
QFQQARALIRFTQSQKYPTVTVGAGVSGNRNSATYAYASPKTSNTYGNFVLPFDVSYEVDAWGRVRQSIEAARTNAQATD
ADLETLRLSFHAELAFDYFEMRSADAEQKLLDDTVVAYRKALDLTENRFAGGVAAGAEVAQAQTQLESTKTLATDVAIRR
AQFEHAIAVLAGKNPSALTIAPKPLAAEPPAIPVGVPSQLLERRPDIAANERRVAEANAEVGIARTAFFPQLLLGATAGL
EGQSITDWINWPSRFWSIGPSAIQTIFDGGRRRATQKAAEFNYDATVATYRQTALDAFQQVEDNLIALRVLEQETQSQRA
AVIAAERSVELSTNRYTGGLVTYLEVVTAQSAALNNERAVVDILRRRMDASVLLIKALGGGWDTSRLPKLAS
>Mature_472_residues
MRLAVALLALLAVGCSVGPKYQRPMIQAPPAYKESQGWKTAQPADTVLRGNWWTLFGDAELNALEQQIDLSNQNLKAAEA
QFQQARALIRFTQSQKYPTVTVGAGVSGNRNSATYAYASPKTSNTYGNFVLPFDVSYEVDAWGRVRQSIEAARTNAQATD
ADLETLRLSFHAELAFDYFEMRSADAEQKLLDDTVVAYRKALDLTENRFAGGVAAGAEVAQAQTQLESTKTLATDVAIRR
AQFEHAIAVLAGKNPSALTIAPKPLAAEPPAIPVGVPSQLLERRPDIAANERRVAEANAEVGIARTAFFPQLLLGATAGL
EGQSITDWINWPSRFWSIGPSAIQTIFDGGRRRATQKAAEFNYDATVATYRQTALDAFQQVEDNLIALRVLEQETQSQRA
AVIAAERSVELSTNRYTGGLVTYLEVVTAQSAALNNERAVVDILRRRMDASVLLIKALGGGWDTSRLPKLAS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=462, Percent_Identity=29.004329004329, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI2367348, Length=470, Percent_Identity=24.0425531914894, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51362; Mature: 51362

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAVALLALLAVGCSVGPKYQRPMIQAPPAYKESQGWKTAQPADTVLRGNWWTLFGDAE
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEECCEEEEECCCH
LNALEQQIDLSNQNLKAAEAQFQQARALIRFTQSQKYPTVTVGAGVSGNRNSATYAYASP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEECC
KTSNTYGNFVLPFDVSYEVDAWGRVRQSIEAARTNAQATDADLETLRLSFHAELAFDYFE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
MRSADAEQKLLDDTVVAYRKALDLTENRFAGGVAAGAEVAQAQTQLESTKTLATDVAIRR
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQFEHAIAVLAGKNPSALTIAPKPLAAEPPAIPVGVPSQLLERRPDIAANERRVAEANAE
HHHHHEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHCCCC
VGIARTAFFPQLLLGATAGLEGQSITDWINWPSRFWSIGPSAIQTIFDGGRRRATQKAAE
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHCCCHHHHHHHHHCHHHHHHHHHHH
FNYDATVATYRQTALDAFQQVEDNLIALRVLEQETQSQRAAVIAAERSVELSTNRYTGGL
CCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHEEEEEECCCEEECCCCCCCHH
VTYLEVVTAQSAALNNERAVVDILRRRMDASVLLIKALGGGWDTSRLPKLAS
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHCCCCCCC
>Mature Secondary Structure
MRLAVALLALLAVGCSVGPKYQRPMIQAPPAYKESQGWKTAQPADTVLRGNWWTLFGDAE
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEECCEEEEECCCH
LNALEQQIDLSNQNLKAAEAQFQQARALIRFTQSQKYPTVTVGAGVSGNRNSATYAYASP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEECC
KTSNTYGNFVLPFDVSYEVDAWGRVRQSIEAARTNAQATDADLETLRLSFHAELAFDYFE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
MRSADAEQKLLDDTVVAYRKALDLTENRFAGGVAAGAEVAQAQTQLESTKTLATDVAIRR
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQFEHAIAVLAGKNPSALTIAPKPLAAEPPAIPVGVPSQLLERRPDIAANERRVAEANAE
HHHHHEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHCCCC
VGIARTAFFPQLLLGATAGLEGQSITDWINWPSRFWSIGPSAIQTIFDGGRRRATQKAAE
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHCCCHHHHHHHHHCHHHHHHHHHHH
FNYDATVATYRQTALDAFQQVEDNLIALRVLEQETQSQRAAVIAAERSVELSTNRYTGGL
CCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHEEEEEECCCEEECCCCCCCHH
VTYLEVVTAQSAALNNERAVVDILRRRMDASVLLIKALGGGWDTSRLPKLAS
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]