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The map label for this gene is metG [H]

Identifier: 116622455

GI number: 116622455

Start: 4241246

End: 4243171

Strand: Reverse

Name: metG [H]

Synonym: Acid_3353

Alternate gene names: 116622455

Gene position: 4243171-4241246 (Counterclockwise)

Preceding gene: 116622456

Following gene: 116622454

Centisome position: 42.58

GC content: 61.16

Gene sequence:

>1926_bases
ATGAAATACTACCTGACGACTCCTCTGTACTACGTAAATGCGGCGCCGCACATCGGGCACACCTACACCACGATGGCTGC
CGAGACGATCGCGCGATTCAAGCGCATGCAGGGATATGACGCGGTCATGTTCACCGGCACGGATGAACACGGCCAGAAAG
TGGAGCGCTCCGCCGAGGCGGCCGGGAAGACGCCGCAGGAGTTCACGGACACGATCGCGCGCGAGTTCCAACAGCAGTGG
GAGAAGCTCAACATCCGCGTGGACCGGACCATCCGCACCACCGATCCGCGTCACCACAAGGTGGTTCAACGCCTGTTCCA
GATGTGCATGGACAACGGCTACATCTATAAGGGCAGCTACAGCGGTCAGTATTGCGTCTACGATGAACTTTACGCCAACG
ACGCCAAGCCCGGCGATCCTTGCCCGATCTGCGGACGCATCACGGAGACCGTCACCGAAGAGAACTATTTCTTCAAGCTG
TCGGCATTCACGGAGAAGCTGCTCGAGCTCTACGAATCCCAGCCCGATTTCATTCAACCGGAGGCACGGCGCAATGAGGT
CATCTCGTTCGTGAAGCAGGGGCTCAACGATCTCTCGATCAGCCGCACGACAATCAAGTGGGGAATCCCGCTGCCGGTCG
AAGGCAACCACGTCTTCTACGTCTGGTTCGACGCGCTGATCGGTTACATGAGCGCCGTGGATGGCGAGGATTTGTGGCCG
GCCGATTTGCATCTGATGGGCAAGGAGATCACGCGCTTCCACGCCGTCTTCTGGCCGGCGTTCCTCATGGCGGGCGGCGT
GCCGCTGCCCAAGCGCATTGTGGCCCACGGCTGGCTGCTTTTCGAGAACGACAAGATGAGCAAGTCGCGCGGCAACATCG
TGCGCTCCGAACCGATCCGGCAGGTGATGGGCGCCGACGCGCTGCGCTACTTCCTGTTGCGCGAAATCGTTTTCGGACAG
GACGGCAGCTTTAGCTACGACGCGCTGATTGGCCGCTACAATTCCGATCTCGCCAACGGGCTGGGCAACCTGGCGAGCCG
CACGCTGACCATGATCAACCAGTATCGCGGCGGCGCCGTTCCGGAAGGCAGCGACGCGGAAATCGCGGGGATTGCGAATC
AGACCATCCGGACGGTACAGGAAACCTTCGACCGCTTCGAATTCTCAAAGGGCCTCGAAGCGGTGTGGGGCCTGATCTCC
GCGGTGGATAAATTCATCGTGCAAAACGCGCCCTGGAAACTCGCGCGCCAGAACGACCAGGAATCGCAGACCTTGTTGAG
CACGACTTTATATACGGCGGCCGAAGCGCTGCGCGTGGCGACGGCGCTGCTCTCGCCGGTGCTTCCCCAGTCGGCGCCGA
AGATTTGGGCGCAGCTTGGCCTGCCCGAATCGATCGAGGCGGTGCGGTTCGCCACGCTCGAGTGGGGCAGCCTGCAGCCG
GGCCAGAAAATTGGTGAAATCGCCGGAGTCTTCCCGCGCATCGAACTGAAGGAAGCGGTAGGCAAGATGCGCGAGTTGGA
AGAACAGGTCACGGCCGAACAGAACAAGCTACTCGGTAAGACGGCGGCGCCCGTGGAACCGGCTGCCCCGGCGACTCCCA
AAATCGCGATCGACGATTTCGTGAAGGTCGATTTGCGCGTGGGACAGGTGGTCTCGGCCGAGCGCGTGAAGGGCTCGGAC
AAGCTGATGCACATGAAAGTGGATATCGGCGAACCGGAGCCGCGGACGATCGTGGCCGGGATCGCCGAGGCTTATTCGCC
CGAGCAGCTTCTCCATCGCAAGGTGGTGATTGTCGCCAACCTGCAGCCGCGCAAACTGAAGGGCATCGAGTCCAATGGGA
TGATCGTCGCGGCATCGGTGGAAGGCGGCAAGCCCGTGCTTACCGGATTCCATGAAGAAATCCCGGTGGGAGCGCGCCTG
AAGTGA

Upstream 100 bases:

>100_bases
CTCCCGAATACGAGGATTGCCGGCGGCTGGCTGCGCAGTCCGGCGTAGCGCTCAAGCACATCATCGCCGAAGCCAATTTC
GCATACCTCAAACAATCCAG

Downstream 100 bases:

>100_bases
CGCTGGTCGATTCCCATTGTCATCTCGATGACGAAAAGTTCGATGCCGACCGCGAGCAGGTGATGGAGCGCGCCCTCGCG
GCCGGTGTGGAGACGATGAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MKYYLTTPLYYVNAAPHIGHTYTTMAAETIARFKRMQGYDAVMFTGTDEHGQKVERSAEAAGKTPQEFTDTIAREFQQQW
EKLNIRVDRTIRTTDPRHHKVVQRLFQMCMDNGYIYKGSYSGQYCVYDELYANDAKPGDPCPICGRITETVTEENYFFKL
SAFTEKLLELYESQPDFIQPEARRNEVISFVKQGLNDLSISRTTIKWGIPLPVEGNHVFYVWFDALIGYMSAVDGEDLWP
ADLHLMGKEITRFHAVFWPAFLMAGGVPLPKRIVAHGWLLFENDKMSKSRGNIVRSEPIRQVMGADALRYFLLREIVFGQ
DGSFSYDALIGRYNSDLANGLGNLASRTLTMINQYRGGAVPEGSDAEIAGIANQTIRTVQETFDRFEFSKGLEAVWGLIS
AVDKFIVQNAPWKLARQNDQESQTLLSTTLYTAAEALRVATALLSPVLPQSAPKIWAQLGLPESIEAVRFATLEWGSLQP
GQKIGEIAGVFPRIELKEAVGKMRELEEQVTAEQNKLLGKTAAPVEPAAPATPKIAIDDFVKVDLRVGQVVSAERVKGSD
KLMHMKVDIGEPEPRTIVAGIAEAYSPEQLLHRKVVIVANLQPRKLKGIESNGMIVAASVEGGKPVLTGFHEEIPVGARL
K

Sequences:

>Translated_641_residues
MKYYLTTPLYYVNAAPHIGHTYTTMAAETIARFKRMQGYDAVMFTGTDEHGQKVERSAEAAGKTPQEFTDTIAREFQQQW
EKLNIRVDRTIRTTDPRHHKVVQRLFQMCMDNGYIYKGSYSGQYCVYDELYANDAKPGDPCPICGRITETVTEENYFFKL
SAFTEKLLELYESQPDFIQPEARRNEVISFVKQGLNDLSISRTTIKWGIPLPVEGNHVFYVWFDALIGYMSAVDGEDLWP
ADLHLMGKEITRFHAVFWPAFLMAGGVPLPKRIVAHGWLLFENDKMSKSRGNIVRSEPIRQVMGADALRYFLLREIVFGQ
DGSFSYDALIGRYNSDLANGLGNLASRTLTMINQYRGGAVPEGSDAEIAGIANQTIRTVQETFDRFEFSKGLEAVWGLIS
AVDKFIVQNAPWKLARQNDQESQTLLSTTLYTAAEALRVATALLSPVLPQSAPKIWAQLGLPESIEAVRFATLEWGSLQP
GQKIGEIAGVFPRIELKEAVGKMRELEEQVTAEQNKLLGKTAAPVEPAAPATPKIAIDDFVKVDLRVGQVVSAERVKGSD
KLMHMKVDIGEPEPRTIVAGIAEAYSPEQLLHRKVVIVANLQPRKLKGIESNGMIVAASVEGGKPVLTGFHEEIPVGARL
K
>Mature_641_residues
MKYYLTTPLYYVNAAPHIGHTYTTMAAETIARFKRMQGYDAVMFTGTDEHGQKVERSAEAAGKTPQEFTDTIAREFQQQW
EKLNIRVDRTIRTTDPRHHKVVQRLFQMCMDNGYIYKGSYSGQYCVYDELYANDAKPGDPCPICGRITETVTEENYFFKL
SAFTEKLLELYESQPDFIQPEARRNEVISFVKQGLNDLSISRTTIKWGIPLPVEGNHVFYVWFDALIGYMSAVDGEDLWP
ADLHLMGKEITRFHAVFWPAFLMAGGVPLPKRIVAHGWLLFENDKMSKSRGNIVRSEPIRQVMGADALRYFLLREIVFGQ
DGSFSYDALIGRYNSDLANGLGNLASRTLTMINQYRGGAVPEGSDAEIAGIANQTIRTVQETFDRFEFSKGLEAVWGLIS
AVDKFIVQNAPWKLARQNDQESQTLLSTTLYTAAEALRVATALLSPVLPQSAPKIWAQLGLPESIEAVRFATLEWGSLQP
GQKIGEIAGVFPRIELKEAVGKMRELEEQVTAEQNKLLGKTAAPVEPAAPATPKIAIDDFVKVDLRVGQVVSAERVKGSD
KLMHMKVDIGEPEPRTIVAGIAEAYSPEQLLHRKVVIVANLQPRKLKGIESNGMIVAASVEGGKPVLTGFHEEIPVGARL
K

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=540, Percent_Identity=34.4444444444444, Blast_Score=310, Evalue=3e-84,
Organism=Homo sapiens, GI14043022, Length=608, Percent_Identity=25.9868421052632, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI4507947, Length=96, Percent_Identity=40.625, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI215490011, Length=81, Percent_Identity=37.037037037037, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI215490009, Length=81, Percent_Identity=37.037037037037, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI45006986, Length=81, Percent_Identity=37.037037037037, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788432, Length=680, Percent_Identity=28.3823529411765, Blast_Score=271, Evalue=9e-74,
Organism=Escherichia coli, GI1789455, Length=107, Percent_Identity=32.7102803738318, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786861, Length=189, Percent_Identity=26.4550264550265, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI212645096, Length=519, Percent_Identity=32.7552986512524, Blast_Score=286, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI71992781, Length=410, Percent_Identity=31.4634146341463, Blast_Score=203, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17540794, Length=545, Percent_Identity=26.9724770642202, Blast_Score=183, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321610, Length=535, Percent_Identity=30.6542056074766, Blast_Score=271, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6321704, Length=554, Percent_Identity=25.4512635379061, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21358483, Length=490, Percent_Identity=31.0204081632653, Blast_Score=256, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24655311, Length=548, Percent_Identity=28.2846715328467, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI21357223, Length=78, Percent_Identity=39.7435897435897, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24651924, Length=81, Percent_Identity=39.5061728395062, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 71513; Mature: 71513

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYYLTTPLYYVNAAPHIGHTYTTMAAETIARFKRMQGYDAVMFTGTDEHGQKVERSAEA
CCEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
AGKTPQEFTDTIAREFQQQWEKLNIRVDRTIRTTDPRHHKVVQRLFQMCMDNGYIYKGSY
CCCCHHHHHHHHHHHHHHHHHHCCEEEECEEECCCCHHHHHHHHHHHHHHCCCEEEECCC
SGQYCVYDELYANDAKPGDPCPICGRITETVTEENYFFKLSAFTEKLLELYESQPDFIQP
CCCEEEEHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCC
EARRNEVISFVKQGLNDLSISRTTIKWGIPLPVEGNHVFYVWFDALIGYMSAVDGEDLWP
HHHHHHHHHHHHHCCHHCEEEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCC
ADLHLMGKEITRFHAVFWPAFLMAGGVPLPKRIVAHGWLLFENDKMSKSRGNIVRSEPIR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCEEEEECCCCCHHCCCCCHHHHHH
QVMGADALRYFLLREIVFGQDGSFSYDALIGRYNSDLANGLGNLASRTLTMINQYRGGAV
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
PEGSDAEIAGIANQTIRTVQETFDRFEFSKGLEAVWGLISAVDKFIVQNAPWKLARQNDQ
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCH
ESQTLLSTTLYTAAEALRVATALLSPVLPQSAPKIWAQLGLPESIEAVRFATLEWGSLQP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHEEEEEECCCCCC
GQKIGEIAGVFPRIELKEAVGKMRELEEQVTAEQNKLLGKTAAPVEPAAPATPKIAIDDF
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHH
VKVDLRVGQVVSAERVKGSDKLMHMKVDIGEPEPRTIVAGIAEAYSPEQLLHRKVVIVAN
HEEHHHHCCHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCHHHHHCCEEEEEEC
LQPRKLKGIESNGMIVAASVEGGKPVLTGFHEEIPVGARLK
CCCHHHCCCCCCCEEEEEECCCCCCCEECCHHHCCCCCCCC
>Mature Secondary Structure
MKYYLTTPLYYVNAAPHIGHTYTTMAAETIARFKRMQGYDAVMFTGTDEHGQKVERSAEA
CCEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
AGKTPQEFTDTIAREFQQQWEKLNIRVDRTIRTTDPRHHKVVQRLFQMCMDNGYIYKGSY
CCCCHHHHHHHHHHHHHHHHHHCCEEEECEEECCCCHHHHHHHHHHHHHHCCCEEEECCC
SGQYCVYDELYANDAKPGDPCPICGRITETVTEENYFFKLSAFTEKLLELYESQPDFIQP
CCCEEEEHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCC
EARRNEVISFVKQGLNDLSISRTTIKWGIPLPVEGNHVFYVWFDALIGYMSAVDGEDLWP
HHHHHHHHHHHHHCCHHCEEEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCC
ADLHLMGKEITRFHAVFWPAFLMAGGVPLPKRIVAHGWLLFENDKMSKSRGNIVRSEPIR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCEEEEECCCCCHHCCCCCHHHHHH
QVMGADALRYFLLREIVFGQDGSFSYDALIGRYNSDLANGLGNLASRTLTMINQYRGGAV
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
PEGSDAEIAGIANQTIRTVQETFDRFEFSKGLEAVWGLISAVDKFIVQNAPWKLARQNDQ
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCH
ESQTLLSTTLYTAAEALRVATALLSPVLPQSAPKIWAQLGLPESIEAVRFATLEWGSLQP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHEEEEEECCCCCC
GQKIGEIAGVFPRIELKEAVGKMRELEEQVTAEQNKLLGKTAAPVEPAAPATPKIAIDDF
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHH
VKVDLRVGQVVSAERVKGSDKLMHMKVDIGEPEPRTIVAGIAEAYSPEQLLHRKVVIVAN
HEEHHHHCCHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCHHHHHCCEEEEEEC
LQPRKLKGIESNGMIVAASVEGGKPVLTGFHEEIPVGARLK
CCCHHHCCCCCCCEEEEEECCCCCCCEECCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]