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The map label for this gene is ispB [H]

Identifier: 116622453

GI number: 116622453

Start: 4239460

End: 4240461

Strand: Reverse

Name: ispB [H]

Synonym: Acid_3351

Alternate gene names: 116622453

Gene position: 4240461-4239460 (Counterclockwise)

Preceding gene: 116622454

Following gene: 116622450

Centisome position: 42.55

GC content: 63.87

Gene sequence:

>1002_bases
ATGAATTTAGGCAAGCTGGGGCAGGCTCTCACAGCTCGGGAAATCTTCGACCTCATCCAGGACGATTTGGAGCAGGTCGA
AAAGAAGATCACGGCGGAATCCGTGGCGTCGGTAGACGCCGTCACGGCGATCGGCCAGTACCTGCAATCGGCGGGCGGTA
AACGCCTGCGCCCCGCGCTGCTGCTGCTTTCGGCGAAGCTCGTGGGTAATGGAGGACCATCGGCGATCCAGTTGGGCGCC
GTGGTGGAACTGATCCACGCCGCGACTCTGGTGCACGACGACGTCATCGATACCGCGCAGACGCGCCGCGGACGTCCCTC
GACCAACGTGAAGTGGGGTAACCACACCTGCGTGCTCGCCGGCGACTGGCTCTACATGCAGGCCTTCCAGATCGCCCTTC
GGGAACGCAGTTTTCAGATGCTGGACCTGCTGATCGGGCTCACGCAGATGATGGTGGAGGGCGAGTTGCTTCAGTTGGAG
CGCATCGGCCGCATCAACGTCAGCGAGGCCGATTGCATGGAGCTGGTGGACCGCAAGACGGCCTGCCTGTTTAGCGTATG
CGCCCGGCTCGGCGGCCTCTGCGGACACACCGACCCGCAAACTCAGGAAAAGCTCGGCGAATACGCGTGGAATCTCGGCA
TGGCCTTCCAGCTGGTGGACGACGTGCTCGACTTCACCTCGCGCGAGAAAGTGCTCGGCAAGCCGGTGGGCGGCGATCTG
CGCGAGGGCAAGGTGACCCTGCCGCTGGTCTACGCGCTGGAGCGCGCCGCACCCGAGGAGCGGAAGCTGGTGGAGACGAT
CCTGGAAGATCGGACCTACGACCGCGTGCCCTTTCAACGAATCCTGGCGCTGCTCGAAAAGTACCAGGGCATCGAGCGCG
TCAAAGAGCGCGCCCAGGCCTTCACGGATAAGGCACGGCAGACGATCGCCGAGTTCCCGGAGTCGCCATACCAGCGAGCC
CTCCTGGCCGTGACGGAGCTGGTGACCGAGCGCGATCACTAG

Upstream 100 bases:

>100_bases
GCGGCACCACAATCGATGCGATTGCCGGGATCACCAGCCGCAATTTTCGGAGCCTGTGTTTGCACGCGCCGATGGCGAAC
AGTTAAACTGGTTAACTTAC

Downstream 100 bases:

>100_bases
GAGCCCAAAAAGCTCTCGCCGCTTGGCGTGCTATTGGGGATGCGTGGACCAGTCCGAAGTTTGCAACCCTTCAATCCGGT
CAAAGTGATGATCTCGCGTG

Product: geranyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNLGKLGQALTAREIFDLIQDDLEQVEKKITAESVASVDAVTAIGQYLQSAGGKRLRPALLLLSAKLVGNGGPSAIQLGA
VVELIHAATLVHDDVIDTAQTRRGRPSTNVKWGNHTCVLAGDWLYMQAFQIALRERSFQMLDLLIGLTQMMVEGELLQLE
RIGRINVSEADCMELVDRKTACLFSVCARLGGLCGHTDPQTQEKLGEYAWNLGMAFQLVDDVLDFTSREKVLGKPVGGDL
REGKVTLPLVYALERAAPEERKLVETILEDRTYDRVPFQRILALLEKYQGIERVKERAQAFTDKARQTIAEFPESPYQRA
LLAVTELVTERDH

Sequences:

>Translated_333_residues
MNLGKLGQALTAREIFDLIQDDLEQVEKKITAESVASVDAVTAIGQYLQSAGGKRLRPALLLLSAKLVGNGGPSAIQLGA
VVELIHAATLVHDDVIDTAQTRRGRPSTNVKWGNHTCVLAGDWLYMQAFQIALRERSFQMLDLLIGLTQMMVEGELLQLE
RIGRINVSEADCMELVDRKTACLFSVCARLGGLCGHTDPQTQEKLGEYAWNLGMAFQLVDDVLDFTSREKVLGKPVGGDL
REGKVTLPLVYALERAAPEERKLVETILEDRTYDRVPFQRILALLEKYQGIERVKERAQAFTDKARQTIAEFPESPYQRA
LLAVTELVTERDH
>Mature_333_residues
MNLGKLGQALTAREIFDLIQDDLEQVEKKITAESVASVDAVTAIGQYLQSAGGKRLRPALLLLSAKLVGNGGPSAIQLGA
VVELIHAATLVHDDVIDTAQTRRGRPSTNVKWGNHTCVLAGDWLYMQAFQIALRERSFQMLDLLIGLTQMMVEGELLQLE
RIGRINVSEADCMELVDRKTACLFSVCARLGGLCGHTDPQTQEKLGEYAWNLGMAFQLVDDVLDFTSREKVLGKPVGGDL
REGKVTLPLVYALERAAPEERKLVETILEDRTYDRVPFQRILALLEKYQGIERVKERAQAFTDKARQTIAEFPESPYQRA
LLAVTELVTERDH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=325, Percent_Identity=32, Blast_Score=145, Evalue=4e-35,
Organism=Escherichia coli, GI1789578, Length=323, Percent_Identity=33.7461300309598, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI1786623, Length=235, Percent_Identity=34.468085106383, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17505681, Length=316, Percent_Identity=30.0632911392405, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6319475, Length=304, Percent_Identity=29.6052631578947, Blast_Score=125, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24651612, Length=329, Percent_Identity=32.2188449848024, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI281365769, Length=285, Percent_Identity=27.3684210526316, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24660002, Length=285, Percent_Identity=27.3684210526316, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 37002; Mature: 37002

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]