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The map label for this gene is polA [C]

Identifier: 116621877

GI number: 116621877

Start: 3495711

End: 3496556

Strand: Direct

Name: polA [C]

Synonym: Acid_2760

Alternate gene names: 116621877

Gene position: 3495711-3496556 (Clockwise)

Preceding gene: 116621873

Following gene: 116621878

Centisome position: 35.08

GC content: 66.67

Gene sequence:

>846_bases
GTGGACGTTCACCTCATCGATGGCACCTACGAATTGTTCCGCCACTACTATGCCCTTCCGTCCGCGCGCGACGCGCACGG
CCTCGAAGTAGCGGCGGCGCGCGGCGTCCTCCGCTCGGTCCTCGGCATGATCCAGGCCGGCGCCACCCACGTTGCGGTCG
CCACCGACCAGGTCATCGAGTCTTTTCGTAATGGGCTATGGCCCGGCTACAAGACAGGAGACGGAATCGAACCCGACCTC
CTCGCCCAGTTCCCACTGCTCGAAGAAGTGCTCGCCGCCGCCGGGGTCGTCGTCTGGCCCATGGTGGAGTTCGAGGCCGA
CGACGCGCTGGCCGCTGGCGCCTCCGTCGCCGCCCGCGACCCTCGCGTGGACCAGGTCATCATCTGCACACCCGATAAGG
ACCTCGGCCAATCCGTGCGCGGCACTCGCGTCGTGCAGTTGAATCGCCGTACCCGCGTGACCTTCGATGAAGCCGCCATC
GTCGCCAAATTTGGCGTCTCGCCCGAGTCGATTCCCGACTATCTCGCGCTTGTAGGTGACGCCGCGGATGGCTATCCGGG
ACTGCCCGGCTGGGGAGCGAAATCGTCGGCCGCCGTCCTCGCCAAATTCCGCCACCTCGAATCCATCCCGCTCGACTGGC
GCGAATGGCGCGTCAATGCATCCAACGCGGGCGCCCTCGCGCACACGCTGGCGGAGCAGCGCGAGCAGGCCATGCTGTTC
CGGACCCTCGCCACCCTCTGCACCGACATACGGCTCTTCGAAGATGTCGATGAACTGCAATGGCGCGGCCCCACCCCGGC
ATTTACGGCCCTCGGCGCGCGCCTCGACGCCGCCAAGGCTTCATAA

Upstream 100 bases:

>100_bases
TCTCCGATCTCTGCGAGAGTAGCACACTAGTTGCCGCTCTTAATTCCGTAAGCGCCGCTGCGATTTGAACTCGACGTCCT
CAATCCGGTAGAGTTGCCCT

Downstream 100 bases:

>100_bases
ACCGGCTCGCTGCCGGCGACCTCTCGATTATTGTTTGGCCCCTCCGCAATGTACTACTGCGACGATTTGTGGGGCAGGCA
TTTTGCCTGCAGCCGCCTTT

Product: 5'-3' exonuclease

Products: diphosphate; DNAn+1

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MDVHLIDGTYELFRHYYALPSARDAHGLEVAAARGVLRSVLGMIQAGATHVAVATDQVIESFRNGLWPGYKTGDGIEPDL
LAQFPLLEEVLAAAGVVVWPMVEFEADDALAAGASVAARDPRVDQVIICTPDKDLGQSVRGTRVVQLNRRTRVTFDEAAI
VAKFGVSPESIPDYLALVGDAADGYPGLPGWGAKSSAAVLAKFRHLESIPLDWREWRVNASNAGALAHTLAEQREQAMLF
RTLATLCTDIRLFEDVDELQWRGPTPAFTALGARLDAAKAS

Sequences:

>Translated_281_residues
MDVHLIDGTYELFRHYYALPSARDAHGLEVAAARGVLRSVLGMIQAGATHVAVATDQVIESFRNGLWPGYKTGDGIEPDL
LAQFPLLEEVLAAAGVVVWPMVEFEADDALAAGASVAARDPRVDQVIICTPDKDLGQSVRGTRVVQLNRRTRVTFDEAAI
VAKFGVSPESIPDYLALVGDAADGYPGLPGWGAKSSAAVLAKFRHLESIPLDWREWRVNASNAGALAHTLAEQREQAMLF
RTLATLCTDIRLFEDVDELQWRGPTPAFTALGARLDAAKAS
>Mature_281_residues
MDVHLIDGTYELFRHYYALPSARDAHGLEVAAARGVLRSVLGMIQAGATHVAVATDQVIESFRNGLWPGYKTGDGIEPDL
LAQFPLLEEVLAAAGVVVWPMVEFEADDALAAGASVAARDPRVDQVIICTPDKDLGQSVRGTRVVQLNRRTRVTFDEAAI
VAKFGVSPESIPDYLALVGDAADGYPGLPGWGAKSSAAVLAKFRHLESIPLDWREWRVNASNAGALAHTLAEQREQAMLF
RTLATLCTDIRLFEDVDELQWRGPTPAFTALGARLDAAKAS

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=263, Percent_Identity=33.0798479087452, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI226510970, Length=225, Percent_Identity=30.6666666666667, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918
- InterPro:   IPR003583 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N [H]

EC number: 2.7.7.7

Molecular weight: Translated: 30333; Mature: 30333

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVHLIDGTYELFRHYYALPSARDAHGLEVAAARGVLRSVLGMIQAGATHVAVATDQVIE
CCEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
SFRNGLWPGYKTGDGIEPDLLAQFPLLEEVLAAAGVVVWPMVEFEADDALAAGASVAARD
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHCCCCHHCCC
PRVDQVIICTPDKDLGQSVRGTRVVQLNRRTRVTFDEAAIVAKFGVSPESIPDYLALVGD
CCCCEEEEECCCHHHHHCCCCCEEEEECCCEEEEEHHHHHHHHCCCCHHHHHHHHHHHCC
AADGYPGLPGWGAKSSAAVLAKFRHLESIPLDWREWRVNASNAGALAHTLAEQREQAMLF
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHEEECCCCCHHHHHHHHHHHHHHHHH
RTLATLCTDIRLFEDVDELQWRGPTPAFTALGARLDAAKAS
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDVHLIDGTYELFRHYYALPSARDAHGLEVAAARGVLRSVLGMIQAGATHVAVATDQVIE
CCEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
SFRNGLWPGYKTGDGIEPDLLAQFPLLEEVLAAAGVVVWPMVEFEADDALAAGASVAARD
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHCCCCHHCCC
PRVDQVIICTPDKDLGQSVRGTRVVQLNRRTRVTFDEAAIVAKFGVSPESIPDYLALVGD
CCCCEEEEECCCHHHHHCCCCCEEEEECCCEEEEEHHHHHHHHCCCCHHHHHHHHHHHCC
AADGYPGLPGWGAKSSAAVLAKFRHLESIPLDWREWRVNASNAGALAHTLAEQREQAMLF
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHEEECCCCCHHHHHHHHHHHHHHHHH
RTLATLCTDIRLFEDVDELQWRGPTPAFTALGARLDAAKAS
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]