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The map label for this gene is clpB [H]

Identifier: 116621825

GI number: 116621825

Start: 3432887

End: 3434062

Strand: Reverse

Name: clpB [H]

Synonym: Acid_2707

Alternate gene names: 116621825

Gene position: 3434062-3432887 (Counterclockwise)

Preceding gene: 116621826

Following gene: 116621824

Centisome position: 34.46

GC content: 56.55

Gene sequence:

>1176_bases
ATGGGGACCTTGAGAAGACAGTTGGATCCGGCGCAGACCGGGACGCTCGCATCGAAGTTGGATTCAGACCTGAGAAAGAG
GATTGTTGGACAAGAGGAAGCGATCCAACAAATCATTCACATTTATCAGACTCATCTCGCCGGCATGAGCAGTCCGGGAC
GGCCCATCGGCAATCTCTTATTTTTGGGGCCGACCGGCTCGGGAAAGACCCGGCTCGTCGAGGCGACCGCCGAAAGTCTG
TTAGGTGACTCTCGTGCTGTGATCAAAATTGATTGCGCCGAGTTCCAGCACAGCCATGAAATTGCGAAATTGATCGGTTC
GCCTCCCGGATATCTCGGACATCGCGAAACACATCCCCTGCTCAGTCAGGAAGTCCTCGACCAATTTCATACCGATCGCA
TCAAGCTAAGCTTTGTGCTTTTCGATGAGATTGAAAAAGCCAGCGACGCCCTCTGGAATCTGCTGCTCGGTATCCTCGAC
AAGGCCACCTTGACGCTCGGCGACAATCGCCACGTCGATTTCACCAATGCCTTGATCTTCATGACCAGCAACTTGGGCGC
GGCCGAAATGAATTCGATCCTCCGGCCGAACCTGGGATTCGCCGCCGGCGAGTGCGAACGCCGTCACGCGGCGGGCATCC
TCGACACCGCGGTCTCCAATAAAGTGGCGCAGGCCGGCATCGAAGCCGCGCGCCGGAAATTTACTCCGGAGTTCATCAAC
CGCATCGATAAGACTGTGGTGTTTCACCCGCTTTGTGCCGACGACCTGCGCAAAATTTTAACCATTGAGTTAAATATGGT
ACAGCAGCGGGTCTTCAGTGCAGCCAATGCGGCGCCTTTCGTCTTTAATCTGACCGAACCCGCAAAGGATTTCCTGCTGC
GCGAAGGCACGGACATGAAGTACGGCGCCCGCCATCTCAAGCGTGCCGTGGATCGCCTTCTCGTGCACCCCATGTCGAAT
CTGATCGCCACCGGCCAGGTACGCGGCGGCGATTTGATCCGCGTCGATTACGATAGCGCGGGCATCCTAACCTTCTTCAA
AGACCAGGAAGACATGCCTGCCCACGTGATGGCACAGATGGTGGACACTTCCATCGGTCTGCCCACCGGAACGTATTCCA
TGGGCGCCGTGGCTGAACCGGCTCGAGGCATCCACGCCAAAACCACCAAACGCTGA

Upstream 100 bases:

>100_bases
AGTAAACAGGCGCACGCGAATTCGGCTTTTAGTTTCAGCCCTCCGTGTCGTCTCGTTCTGGCCATTAACTTGCTAGTCAA
TGATTGTGAGGGGAGCCGAT

Downstream 100 bases:

>100_bases
TTGCGCTCCATCAATTCCGCTCCCTTACGGAAGCGCTCCCTCAGAGCCGCGACCGTAAGGGAGCGATCTAATACCACGTA
AATCTTTGTTTTCATAACAC

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MGTLRRQLDPAQTGTLASKLDSDLRKRIVGQEEAIQQIIHIYQTHLAGMSSPGRPIGNLLFLGPTGSGKTRLVEATAESL
LGDSRAVIKIDCAEFQHSHEIAKLIGSPPGYLGHRETHPLLSQEVLDQFHTDRIKLSFVLFDEIEKASDALWNLLLGILD
KATLTLGDNRHVDFTNALIFMTSNLGAAEMNSILRPNLGFAAGECERRHAAGILDTAVSNKVAQAGIEAARRKFTPEFIN
RIDKTVVFHPLCADDLRKILTIELNMVQQRVFSAANAAPFVFNLTEPAKDFLLREGTDMKYGARHLKRAVDRLLVHPMSN
LIATGQVRGGDLIRVDYDSAGILTFFKDQEDMPAHVMAQMVDTSIGLPTGTYSMGAVAEPARGIHAKTTKR

Sequences:

>Translated_391_residues
MGTLRRQLDPAQTGTLASKLDSDLRKRIVGQEEAIQQIIHIYQTHLAGMSSPGRPIGNLLFLGPTGSGKTRLVEATAESL
LGDSRAVIKIDCAEFQHSHEIAKLIGSPPGYLGHRETHPLLSQEVLDQFHTDRIKLSFVLFDEIEKASDALWNLLLGILD
KATLTLGDNRHVDFTNALIFMTSNLGAAEMNSILRPNLGFAAGECERRHAAGILDTAVSNKVAQAGIEAARRKFTPEFIN
RIDKTVVFHPLCADDLRKILTIELNMVQQRVFSAANAAPFVFNLTEPAKDFLLREGTDMKYGARHLKRAVDRLLVHPMSN
LIATGQVRGGDLIRVDYDSAGILTFFKDQEDMPAHVMAQMVDTSIGLPTGTYSMGAVAEPARGIHAKTTKR
>Mature_390_residues
GTLRRQLDPAQTGTLASKLDSDLRKRIVGQEEAIQQIIHIYQTHLAGMSSPGRPIGNLLFLGPTGSGKTRLVEATAESLL
GDSRAVIKIDCAEFQHSHEIAKLIGSPPGYLGHRETHPLLSQEVLDQFHTDRIKLSFVLFDEIEKASDALWNLLLGILDK
ATLTLGDNRHVDFTNALIFMTSNLGAAEMNSILRPNLGFAAGECERRHAAGILDTAVSNKVAQAGIEAARRKFTPEFINR
IDKTVVFHPLCADDLRKILTIELNMVQQRVFSAANAAPFVFNLTEPAKDFLLREGTDMKYGARHLKRAVDRLLVHPMSNL
IATGQVRGGDLIRVDYDSAGILTFFKDQEDMPAHVMAQMVDTSIGLPTGTYSMGAVAEPARGIHAKTTKR

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=327, Percent_Identity=30.2752293577982, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1788943, Length=325, Percent_Identity=34.1538461538462, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787109, Length=319, Percent_Identity=28.5266457680251, Blast_Score=135, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6320464, Length=316, Percent_Identity=39.873417721519, Blast_Score=210, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6323002, Length=316, Percent_Identity=30.6962025316456, Blast_Score=158, Evalue=1e-39,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 42924; Mature: 42793

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTLRRQLDPAQTGTLASKLDSDLRKRIVGQEEAIQQIIHIYQTHLAGMSSPGRPIGNLL
CCCHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
FLGPTGSGKTRLVEATAESLLGDSRAVIKIDCAEFQHSHEIAKLIGSPPGYLGHRETHPL
EECCCCCCCCEEHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
LSQEVLDQFHTDRIKLSFVLFDEIEKASDALWNLLLGILDKATLTLGDNRHVDFTNALIF
HHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEHHCEEEE
MTSNLGAAEMNSILRPNLGFAAGECERRHAAGILDTAVSNKVAQAGIEAARRKFTPEFIN
EECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RIDKTVVFHPLCADDLRKILTIELNMVQQRVFSAANAAPFVFNLTEPAKDFLLREGTDMK
HHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCH
YGARHLKRAVDRLLVHPMSNLIATGQVRGGDLIRVDYDSAGILTFFKDQEDMPAHVMAQM
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHH
VDTSIGLPTGTYSMGAVAEPARGIHAKTTKR
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GTLRRQLDPAQTGTLASKLDSDLRKRIVGQEEAIQQIIHIYQTHLAGMSSPGRPIGNLL
CCHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
FLGPTGSGKTRLVEATAESLLGDSRAVIKIDCAEFQHSHEIAKLIGSPPGYLGHRETHPL
EECCCCCCCCEEHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
LSQEVLDQFHTDRIKLSFVLFDEIEKASDALWNLLLGILDKATLTLGDNRHVDFTNALIF
HHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEHHCEEEE
MTSNLGAAEMNSILRPNLGFAAGECERRHAAGILDTAVSNKVAQAGIEAARRKFTPEFIN
EECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RIDKTVVFHPLCADDLRKILTIELNMVQQRVFSAANAAPFVFNLTEPAKDFLLREGTDMK
HHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCH
YGARHLKRAVDRLLVHPMSNLIATGQVRGGDLIRVDYDSAGILTFFKDQEDMPAHVMAQM
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHH
VDTSIGLPTGTYSMGAVAEPARGIHAKTTKR
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA