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The map label for this gene is hemB [H]

Identifier: 116621576

GI number: 116621576

Start: 3124043

End: 3125017

Strand: Reverse

Name: hemB [H]

Synonym: Acid_2458

Alternate gene names: 116621576

Gene position: 3125017-3124043 (Counterclockwise)

Preceding gene: 116621577

Following gene: 116621575

Centisome position: 31.36

GC content: 62.77

Gene sequence:

>975_bases
GTGATGCCGTTTCCTATTCATCGATTACGCCGATTGAGGGCTACCGAGCCGCTGCGCAACCTGGTGCGGGAGACGCACCT
GAATCCGGCGCAGTTTATTTTGCCGCTGTTCGTGTGCCCGGGCGAGGGCGTGAAGCGCGAGATCAGCTCGATGCCGGACA
ACTACCAGATGTCGATCGATGAGATCGTTCGCGAGTGCGAGGTAGTGGAAGCGCTGGGGATCGGCGGGGTGATTCTGTTC
GGGCTGCCCGAGACCAAGGACGAGATGGCGTCTGGGGCGTACGACGATAACGGAATCGTGCAGCACGCGATCCGGGCGAT
CAAGCGCGAGACCAAGAAGCTGCTGGTGATCACGGATGTGTGCAACTGCGAGTACACGAGCCACGGGCATTGCGGATATG
TGAAAGACGGCGATGTGGATAACGACACAACGCTGCAATGGCTGGCGAAAGCGGCGCTGTCGCACGCGCGGGCGGGGGCG
GACATTGTGGCGCCCTCGGACATGATGGACGGGCGGGTGTGGGCGATCCGGCAGGCGCTGGACGCGAACGGGTTCGACCG
GACGCCGATCATGAGCTACGCGGCGAAGTTCGCGAGCGTGTTTTACGGACCGTTCCGCGAGGCGGCGGAATCGGCGCCGC
AGTTCGGGGACCGGCGGAGCTACCAGATGGATCCGGCGAACGGGCGGGAAGCGATGCGGGAGATCGAGCTGGATCTGGAG
GAAGGCGCGGACATGATCATGGTGAAGCCGGCGCTTTCGTATCTGGATTTGATCGCGGAGGCGCGGCGGCGCTTCGACGT
CCCGATCGCGGCCTACCAGGTGAGCGGCGAGTACTCGATGATCATCGCGGCGGCGCGCAATGGATGGATCGACCTCGACC
GGGCCATGATGGAGACCTTAACTTCGATTACGCGGGCGGGAGCGAAGATCATATTGACGTATTTCGCAAAGCCGGCGGCA
AGGATGTTGGGATGA

Upstream 100 bases:

>100_bases
GCGGATATTCCGGTGCGGACGTTGCGGCCGGCGAGCAAACAAGAGCAGTGTAATGGTGAGATGAGCGGGAGGCGTATGCT
ACCAGGAGTTACAATCCTGA

Downstream 100 bases:

>100_bases
TTTCGCGACGGATGTTTGTGGGAGCGATGGCGGCGGCGCCCTTAGTGACGACGCCGTTAATGGCGGCCGACGAGCCGTGG
ATCCCGTTATTCAACGGGAA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MMPFPIHRLRRLRATEPLRNLVRETHLNPAQFILPLFVCPGEGVKREISSMPDNYQMSIDEIVRECEVVEALGIGGVILF
GLPETKDEMASGAYDDNGIVQHAIRAIKRETKKLLVITDVCNCEYTSHGHCGYVKDGDVDNDTTLQWLAKAALSHARAGA
DIVAPSDMMDGRVWAIRQALDANGFDRTPIMSYAAKFASVFYGPFREAAESAPQFGDRRSYQMDPANGREAMREIELDLE
EGADMIMVKPALSYLDLIAEARRRFDVPIAAYQVSGEYSMIIAAARNGWIDLDRAMMETLTSITRAGAKIILTYFAKPAA
RMLG

Sequences:

>Translated_324_residues
MMPFPIHRLRRLRATEPLRNLVRETHLNPAQFILPLFVCPGEGVKREISSMPDNYQMSIDEIVRECEVVEALGIGGVILF
GLPETKDEMASGAYDDNGIVQHAIRAIKRETKKLLVITDVCNCEYTSHGHCGYVKDGDVDNDTTLQWLAKAALSHARAGA
DIVAPSDMMDGRVWAIRQALDANGFDRTPIMSYAAKFASVFYGPFREAAESAPQFGDRRSYQMDPANGREAMREIELDLE
EGADMIMVKPALSYLDLIAEARRRFDVPIAAYQVSGEYSMIIAAARNGWIDLDRAMMETLTSITRAGAKIILTYFAKPAA
RMLG
>Mature_324_residues
MMPFPIHRLRRLRATEPLRNLVRETHLNPAQFILPLFVCPGEGVKREISSMPDNYQMSIDEIVRECEVVEALGIGGVILF
GLPETKDEMASGAYDDNGIVQHAIRAIKRETKKLLVITDVCNCEYTSHGHCGYVKDGDVDNDTTLQWLAKAALSHARAGA
DIVAPSDMMDGRVWAIRQALDANGFDRTPIMSYAAKFASVFYGPFREAAESAPQFGDRRSYQMDPANGREAMREIELDLE
EGADMIMVKPALSYLDLIAEARRRFDVPIAAYQVSGEYSMIIAAARNGWIDLDRAMMETLTSITRAGAKIILTYFAKPAA
RMLG

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=296, Percent_Identity=43.9189189189189, Blast_Score=245, Evalue=5e-65,
Organism=Escherichia coli, GI87081728, Length=311, Percent_Identity=50.1607717041801, Blast_Score=286, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6321398, Length=305, Percent_Identity=43.2786885245902, Blast_Score=234, Evalue=1e-62,
Organism=Drosophila melanogaster, GI21358291, Length=290, Percent_Identity=41.7241379310345, Blast_Score=236, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36041; Mature: 36041

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPFPIHRLRRLRATEPLRNLVRETHLNPAQFILPLFVCPGEGVKREISSMPDNYQMSID
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHH
EIVRECEVVEALGIGGVILFGLPETKDEMASGAYDDNGIVQHAIRAIKRETKKLLVITDV
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEEEC
CNCEYTSHGHCGYVKDGDVDNDTTLQWLAKAALSHARAGADIVAPSDMMDGRVWAIRQAL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHH
DANGFDRTPIMSYAAKFASVFYGPFREAAESAPQFGDRRSYQMDPANGREAMREIELDLE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
EGADMIMVKPALSYLDLIAEARRRFDVPIAAYQVSGEYSMIIAAARNGWIDLDRAMMETL
CCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCHHHHHHHHHH
TSITRAGAKIILTYFAKPAARMLG
HHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMPFPIHRLRRLRATEPLRNLVRETHLNPAQFILPLFVCPGEGVKREISSMPDNYQMSID
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHH
EIVRECEVVEALGIGGVILFGLPETKDEMASGAYDDNGIVQHAIRAIKRETKKLLVITDV
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEEEC
CNCEYTSHGHCGYVKDGDVDNDTTLQWLAKAALSHARAGADIVAPSDMMDGRVWAIRQAL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHH
DANGFDRTPIMSYAAKFASVFYGPFREAAESAPQFGDRRSYQMDPANGREAMREIELDLE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
EGADMIMVKPALSYLDLIAEARRRFDVPIAAYQVSGEYSMIIAAARNGWIDLDRAMMETL
CCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCHHHHHHHHHH
TSITRAGAKIILTYFAKPAARMLG
HHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8123787 [H]