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The map label for this gene is rlmI [H]

Identifier: 116621453

GI number: 116621453

Start: 2979567

End: 2980730

Strand: Direct

Name: rlmI [H]

Synonym: Acid_2335

Alternate gene names: 116621453

Gene position: 2979567-2980730 (Clockwise)

Preceding gene: 116621452

Following gene: 116621456

Centisome position: 29.9

GC content: 65.21

Gene sequence:

>1164_bases
ATGAACGAAGTCCGGGTGAACCGCAAGGCGGCCGGCCGGGTCGCCGGCGGCCATCCCTGGATTTTCGCCAGTGATATTGA
CGATCGCGATGGCGCGCAGGGCGGCCACGCGGTCAAAGTCGCCGACCAGCGCGGCCATCCGCTGGGGACAGCTCATTACA
GCTCGGCTTCGCAGATCACCTTGCGGATGCTATCGCGGCAGATCGAAGAGATCGGCCGCGATTTCTATTTGCACCGGCTG
CGCGCCGCCGAACAGCACCGCCGCACCGTAGTCCACGATAGCGATGCCTACCGCGTGGTCCACGGCGAGGCCGACCTTTT
GCCCGCGCTGGTGGTGGATCGCTATGGCGATTACCTGGTGATGCAGACGCTCGACCAGGGCATGGACGCGGCCAAGGCCG
AGATCGCATCGTGCCTGGAAGAGATCTTCCGGCCCAAGGGAATCGTCGCGCGCAACGACGTCTCGGTGCGCACGAAGGAA
GAGCTGCCGCTCGAATCCGGGATCGTCGCGGGCGATGTTCCAGAATCGGTGACGGTGCGGATGAACGGGCTGACGCTGCG
CGCCGACCTGCTTCACGGACAGAAGACCGGGATTTTTCTGGATCAGCGGCAGAACTATCGTGCGGCCGCGCAGTACGCAC
GGGGAGGACGGGCGCTCGATTGCTTCACCTCCACGGGCGGGTTCGCGCTCCATCTCGCGCCCAAGTGTGAACACGTGGAG
GCCGTGGATTCCTCGGGCCCCGCGCTGGAGGCGGCCCGCGCTAATTCCACGGCCAACGGTATCGACAACATCGATTGGAA
CGAGGCCGACGTCTTCGACCTGCTCTCCGGCTACGCGAACGCGCGGCGACAGTTCTCCATGGTGGTGCTGGACCCGCCCG
CGTTCGCCAAGAATAAGCGCAGTCTCGACCCGGCCATCAGCGCGTACAAGGAGATCAACCTTCGGGCGATGCGCCTGTTG
CAGCCCGGCGGAATCCTGGTGACCTGCTCCTGCTCGCACCACCTCAGTGAAGCCTCGCTGCTCGAACTCGTGGCCGGGGC
CGGCCTGGACACGCACCGCACCCTGCGGGTGCTCGAGCGCCGCTCGCAGAGCCAGGATCATCCCATCCTGCTGACCGTGC
CGGAGACCCTCTATCTGAAGTGCCTGATTCTGGAAGTGGTGTAG

Upstream 100 bases:

>100_bases
ACATGTTCATCATCAAGCCCTTCAGCGGACAGAGCCTCATGAAGCTCTTCTCCACGCACCCCGCCACGGAAGATCGCATC
GCCCGCCTTCAGGAGATGCG

Downstream 100 bases:

>100_bases
GACGAACCGCGACCGCAGTCGAGCGAAGCTCGCTGGGTCCGGCTCGTCGGCGCTACTGCTCGCTGTAGTTCCAGAGGAGG
CCGCCATCGACGACGATGGT

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MNEVRVNRKAAGRVAGGHPWIFASDIDDRDGAQGGHAVKVADQRGHPLGTAHYSSASQITLRMLSRQIEEIGRDFYLHRL
RAAEQHRRTVVHDSDAYRVVHGEADLLPALVVDRYGDYLVMQTLDQGMDAAKAEIASCLEEIFRPKGIVARNDVSVRTKE
ELPLESGIVAGDVPESVTVRMNGLTLRADLLHGQKTGIFLDQRQNYRAAAQYARGGRALDCFTSTGGFALHLAPKCEHVE
AVDSSGPALEAARANSTANGIDNIDWNEADVFDLLSGYANARRQFSMVVLDPPAFAKNKRSLDPAISAYKEINLRAMRLL
QPGGILVTCSCSHHLSEASLLELVAGAGLDTHRTLRVLERRSQSQDHPILLTVPETLYLKCLILEVV

Sequences:

>Translated_387_residues
MNEVRVNRKAAGRVAGGHPWIFASDIDDRDGAQGGHAVKVADQRGHPLGTAHYSSASQITLRMLSRQIEEIGRDFYLHRL
RAAEQHRRTVVHDSDAYRVVHGEADLLPALVVDRYGDYLVMQTLDQGMDAAKAEIASCLEEIFRPKGIVARNDVSVRTKE
ELPLESGIVAGDVPESVTVRMNGLTLRADLLHGQKTGIFLDQRQNYRAAAQYARGGRALDCFTSTGGFALHLAPKCEHVE
AVDSSGPALEAARANSTANGIDNIDWNEADVFDLLSGYANARRQFSMVVLDPPAFAKNKRSLDPAISAYKEINLRAMRLL
QPGGILVTCSCSHHLSEASLLELVAGAGLDTHRTLRVLERRSQSQDHPILLTVPETLYLKCLILEVV
>Mature_387_residues
MNEVRVNRKAAGRVAGGHPWIFASDIDDRDGAQGGHAVKVADQRGHPLGTAHYSSASQITLRMLSRQIEEIGRDFYLHRL
RAAEQHRRTVVHDSDAYRVVHGEADLLPALVVDRYGDYLVMQTLDQGMDAAKAEIASCLEEIFRPKGIVARNDVSVRTKE
ELPLESGIVAGDVPESVTVRMNGLTLRADLLHGQKTGIFLDQRQNYRAAAQYARGGRALDCFTSTGGFALHLAPKCEHVE
AVDSSGPALEAARANSTANGIDNIDWNEADVFDLLSGYANARRQFSMVVLDPPAFAKNKRSLDPAISAYKEINLRAMRLL
QPGGILVTCSCSHHLSEASLLELVAGAGLDTHRTLRVLERRSQSQDHPILLTVPETLYLKCLILEVV

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=378, Percent_Identity=37.037037037037, Blast_Score=253, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 42429; Mature: 42429

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVRVNRKAAGRVAGGHPWIFASDIDDRDGAQGGHAVKVADQRGHPLGTAHYSSASQIT
CCCCCCCHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHH
LRMLSRQIEEIGRDFYLHRLRAAEQHRRTVVHDSDAYRVVHGEADLLPALVVDRYGDYLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEECCHHHHHHHHHHHCCCEEE
MQTLDQGMDAAKAEIASCLEEIFRPKGIVARNDVSVRTKEELPLESGIVAGDVPESVTVR
HHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCEEECCCCCHHCCEEECCCCCCEEEE
MNGLTLRADLLHGQKTGIFLDQRQNYRAAAQYARGGRALDCFTSTGGFALHLAPKCEHVE
ECCEEEEEEHHCCCCCCEEEECCCCHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCHHH
AVDSSGPALEAARANSTANGIDNIDWNEADVFDLLSGYANARRQFSMVVLDPPAFAKNKR
HCCCCCCCEEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCC
SLDPAISAYKEINLRAMRLLQPGGILVTCSCSHHLSEASLLELVAGAGLDTHRTLRVLER
CCCHHHHHHHHHCHHHEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
RSQSQDHPILLTVPETLYLKCLILEVV
HCCCCCCCEEEECCHHHHHHHHEEECC
>Mature Secondary Structure
MNEVRVNRKAAGRVAGGHPWIFASDIDDRDGAQGGHAVKVADQRGHPLGTAHYSSASQIT
CCCCCCCHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHH
LRMLSRQIEEIGRDFYLHRLRAAEQHRRTVVHDSDAYRVVHGEADLLPALVVDRYGDYLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEECCHHHHHHHHHHHCCCEEE
MQTLDQGMDAAKAEIASCLEEIFRPKGIVARNDVSVRTKEELPLESGIVAGDVPESVTVR
HHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCEEECCCCCHHCCEEECCCCCCEEEE
MNGLTLRADLLHGQKTGIFLDQRQNYRAAAQYARGGRALDCFTSTGGFALHLAPKCEHVE
ECCEEEEEEHHCCCCCCEEEECCCCHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCHHH
AVDSSGPALEAARANSTANGIDNIDWNEADVFDLLSGYANARRQFSMVVLDPPAFAKNKR
HCCCCCCCEEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCC
SLDPAISAYKEINLRAMRLLQPGGILVTCSCSHHLSEASLLELVAGAGLDTHRTLRVLER
CCCHHHHHHHHHCHHHEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
RSQSQDHPILLTVPETLYLKCLILEVV
HCCCCCCCEEEECCHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA