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The map label for this gene is gutB [H]

Identifier: 116621341

GI number: 116621341

Start: 2844869

End: 2845888

Strand: Direct

Name: gutB [H]

Synonym: Acid_2222

Alternate gene names: 116621341

Gene position: 2844869-2845888 (Clockwise)

Preceding gene: 116621340

Following gene: 116621342

Centisome position: 28.55

GC content: 67.55

Gene sequence:

>1020_bases
ATGCGTGCGGCATTCTTTGAAGGCAATCGGACCTTGCGCGTCGGCGACTGCGCGGAGGTGGCTCCTGGCGCGGGACAAGT
GCAGATAAACGTGGAATTCTGCGGCATCTGCGGCACGGACCTTCACCTGTTTCACGGCGCCATGGCGCACCGGCTCACGC
TGCCCCACGTGATGGGACACGAGATGTCGGGCACGCTGGCGGCGGTGGGGGAAGGCGTTGCCGGATGGCAGGCGGGTGAT
CGCGTCACCGTGCGGCCGCTGGACCCGTGCGGCACATGTCCGGCCTGCCGGATGGGCCACAGCCACATTTGCCACAATCT
CAAATTCATCGGCATCGATACGCCGGGCGCGCTGCAGGGCATGTGGACGGTGCCTGCGCACACGCTCCACCGGCTGCCCG
ATTCGCTCTCGCTGCGCGAGGGCGCGATGGTGGAACCGATCGCGGTGGCCTGCCACGACGTGCGCATGAGCGAACTGCAG
GCCGGCGAGTTCGCGGTCGTGATCGGCGGCGGACCGATCGGGATCCTGATCGCGCTCGTGGCGAAGGCGCGCGGCGCGCG
AGTGCTGCAGGCGGAAGTGAACCCGTTCCGGCTGCAATTGGCCCGCGATCTCGGAATCGACGCGGTGAACCCGGCGGAGA
CGGACCTGCCGGCGCTGGTGAACCAGCACACGGCCGGCGCGGGCGCGGACGTGGTCTTCGAAGTTTCGGGGTCCTCAGCG
GGCTGCGAAATGATGACCAGGCTGCCGCGCACGCGCGGGCGCATCGTGGTGGTGGCGATCTACGCCGAGCCGCAGAAGGT
GGACCTGTTCCGCTTTTTCTGGCGGGAGCTCCGACTCGCCGGAGCGCGGGTCTACGAGCCCGAGGATTTCGACGGCGCGA
TCGCCCTCGCAGCATCAGGCAAACTGCCGCTCGACCGCCTGATTACGGACGTGCTGCCCCTCAGCCGGCTGGAAGAAGCC
ATGCACCGGATGGAGCGCGGCGGCGACTGCATGAAGATCCTGGTAAGTTGTACGGACTAA

Upstream 100 bases:

>100_bases
CCATGCGGCTTCCGCACGGCGCTACCATCGCGGTCGGCGCGCTTTTCTTTCTGGCCATGAGCGCGCGATTTACCCGGTAA
ACAAAGGACACGGCGAAAAT

Downstream 100 bases:

>100_bases
TTACGTGAGCATACTCGATAAATTCAAGGTGAGCGGTAAGACCGCACTCGTCACCGGCGCCCGTCGCGGCATCGGCAAGG
CAATGGCGGTGGCCCTGGCG

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MRAAFFEGNRTLRVGDCAEVAPGAGQVQINVEFCGICGTDLHLFHGAMAHRLTLPHVMGHEMSGTLAAVGEGVAGWQAGD
RVTVRPLDPCGTCPACRMGHSHICHNLKFIGIDTPGALQGMWTVPAHTLHRLPDSLSLREGAMVEPIAVACHDVRMSELQ
AGEFAVVIGGGPIGILIALVAKARGARVLQAEVNPFRLQLARDLGIDAVNPAETDLPALVNQHTAGAGADVVFEVSGSSA
GCEMMTRLPRTRGRIVVVAIYAEPQKVDLFRFFWRELRLAGARVYEPEDFDGAIALAASGKLPLDRLITDVLPLSRLEEA
MHRMERGGDCMKILVSCTD

Sequences:

>Translated_339_residues
MRAAFFEGNRTLRVGDCAEVAPGAGQVQINVEFCGICGTDLHLFHGAMAHRLTLPHVMGHEMSGTLAAVGEGVAGWQAGD
RVTVRPLDPCGTCPACRMGHSHICHNLKFIGIDTPGALQGMWTVPAHTLHRLPDSLSLREGAMVEPIAVACHDVRMSELQ
AGEFAVVIGGGPIGILIALVAKARGARVLQAEVNPFRLQLARDLGIDAVNPAETDLPALVNQHTAGAGADVVFEVSGSSA
GCEMMTRLPRTRGRIVVVAIYAEPQKVDLFRFFWRELRLAGARVYEPEDFDGAIALAASGKLPLDRLITDVLPLSRLEEA
MHRMERGGDCMKILVSCTD
>Mature_339_residues
MRAAFFEGNRTLRVGDCAEVAPGAGQVQINVEFCGICGTDLHLFHGAMAHRLTLPHVMGHEMSGTLAAVGEGVAGWQAGD
RVTVRPLDPCGTCPACRMGHSHICHNLKFIGIDTPGALQGMWTVPAHTLHRLPDSLSLREGAMVEPIAVACHDVRMSELQ
AGEFAVVIGGGPIGILIALVAKARGARVLQAEVNPFRLQLARDLGIDAVNPAETDLPALVNQHTAGAGADVVFEVSGSSA
GCEMMTRLPRTRGRIVVVAIYAEPQKVDLFRFFWRELRLAGARVYEPEDFDGAIALAASGKLPLDRLITDVLPLSRLEEA
MHRMERGGDCMKILVSCTD

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=335, Percent_Identity=30.1492537313433, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI4501929, Length=371, Percent_Identity=26.4150943396226, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI156523966, Length=354, Percent_Identity=25.7062146892655, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI34577061, Length=368, Percent_Identity=26.0869565217391, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI4501939, Length=346, Percent_Identity=26.0115606936416, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI4501933, Length=370, Percent_Identity=24.8648648648649, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=23.5135135135135, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=23.5135135135135, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI71565154, Length=265, Percent_Identity=24.5283018867925, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1790045, Length=320, Percent_Identity=32.1875, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI226510992, Length=304, Percent_Identity=31.25, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788073, Length=337, Percent_Identity=28.7833827893175, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787863, Length=343, Percent_Identity=26.530612244898, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI87082125, Length=357, Percent_Identity=29.4117647058824, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788407, Length=320, Percent_Identity=25.625, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788075, Length=320, Percent_Identity=27.8125, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1790718, Length=297, Percent_Identity=28.2828282828283, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI87081918, Length=307, Percent_Identity=28.6644951140065, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786825, Length=276, Percent_Identity=27.1739130434783, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562876, Length=345, Percent_Identity=32.7536231884058, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17562878, Length=345, Percent_Identity=34.2028985507246, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71988145, Length=320, Percent_Identity=24.6875, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17562584, Length=322, Percent_Identity=24.8447204968944, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17562582, Length=321, Percent_Identity=25.8566978193146, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6322619, Length=323, Percent_Identity=30.3405572755418, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319955, Length=323, Percent_Identity=30.3405572755418, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323099, Length=298, Percent_Identity=33.5570469798658, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319257, Length=343, Percent_Identity=28.5714285714286, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323961, Length=331, Percent_Identity=31.1178247734139, Blast_Score=129, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6319258, Length=347, Percent_Identity=26.2247838616715, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6324486, Length=334, Percent_Identity=31.7365269461078, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6323729, Length=328, Percent_Identity=27.4390243902439, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6319621, Length=331, Percent_Identity=26.8882175226586, Blast_Score=106, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17137530, Length=321, Percent_Identity=30.5295950155763, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17737897, Length=321, Percent_Identity=31.4641744548287, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17737895, Length=372, Percent_Identity=25.5376344086022, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI221457811, Length=347, Percent_Identity=23.6311239193084, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45550770, Length=347, Percent_Identity=23.6311239193084, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45551930, Length=347, Percent_Identity=23.6311239193084, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 36309; Mature: 36309

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00028 ZINC_FINGER_C2H2_1 ; PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAAFFEGNRTLRVGDCAEVAPGAGQVQINVEFCGICGTDLHLFHGAMAHRLTLPHVMGH
CCCEEECCCCEEEECCHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHCCCCHHHCC
EMSGTLAAVGEGVAGWQAGDRVTVRPLDPCGTCPACRMGHSHICHNLKFIGIDTPGALQG
CCCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCHHHHCCCEEEEECCCCCCCC
MWTVPAHTLHRLPDSLSLREGAMVEPIAVACHDVRMSELQAGEFAVVIGGGPIGILIALV
EEECCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
AKARGARVLQAEVNPFRLQLARDLGIDAVNPAETDLPALVNQHTAGAGADVVFEVSGSSA
HHCCCCEEEEECCCCEEEEEHHHCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCC
GCEMMTRLPRTRGRIVVVAIYAEPQKVDLFRFFWRELRLAGARVYEPEDFDGAIALAASG
CHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEEECC
KLPLDRLITDVLPLSRLEEAMHRMERGGDCMKILVSCTD
CCCHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEECC
>Mature Secondary Structure
MRAAFFEGNRTLRVGDCAEVAPGAGQVQINVEFCGICGTDLHLFHGAMAHRLTLPHVMGH
CCCEEECCCCEEEECCHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHCCCCHHHCC
EMSGTLAAVGEGVAGWQAGDRVTVRPLDPCGTCPACRMGHSHICHNLKFIGIDTPGALQG
CCCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCHHHHCCCEEEEECCCCCCCC
MWTVPAHTLHRLPDSLSLREGAMVEPIAVACHDVRMSELQAGEFAVVIGGGPIGILIALV
EEECCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
AKARGARVLQAEVNPFRLQLARDLGIDAVNPAETDLPALVNQHTAGAGADVVFEVSGSSA
HHCCCCEEEEECCCCEEEEEHHHCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCC
GCEMMTRLPRTRGRIVVVAIYAEPQKVDLFRFFWRELRLAGARVYEPEDFDGAIALAASG
CHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEEECC
KLPLDRLITDVLPLSRLEEAMHRMERGGDCMKILVSCTD
CCCHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]