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The map label for this gene is ywlF [H]

Identifier: 116621323

GI number: 116621323

Start: 2825103

End: 2825603

Strand: Direct

Name: ywlF [H]

Synonym: Acid_2204

Alternate gene names: 116621323

Gene position: 2825103-2825603 (Clockwise)

Preceding gene: 116621321

Following gene: 116621324

Centisome position: 28.35

GC content: 61.48

Gene sequence:

>501_bases
GTGGCGGAAGACCAGTTGACGGCGCTGGCGCCGCCGGAGCGGACCATCGCGATCGGCAGCGATCACGGCGGGTTCCGGCT
GAAGGAAGCGTTGAAACCGCTGCTCGAAGGGCTGGGACTGCAAGTGCGCGACGTGGGCGTAAACGAAGAGAAGCCGGCGG
ACTATCCGGATATCGCGCACGCGGTGGCGAAACTGGTGGCGGGCGGGACGGCGGCGCGCGGTGTGATTATCGATGGCGCG
GGGATCGGCTCGTGCATGGCGGCGAACAAGGTTCCGGGCATTCGCGCGGCCCTGTGTTATGATAAAGCTTCCGCTAAGAA
TGGCCGCGAACATAATGATTCCAATGTACTTACGTTGGGTGCGCGGCTCCTGACACAGACCCAGGCGGAAGAGGTTTTAC
GCACCTGGCTGGAGACGCCGTTTGCCGGTGGAAGGCATCAGGCGCGGGTCCAAAAAATCATGGACGTAGAACAGCAATAT
TCAAAGAAGGCATCCTCGTGA

Upstream 100 bases:

>100_bases
GGATGTGCCGGCGGGCGGTGAGTTACGCGTTCCGGTGGGAACGATCGTGACCTCGTCGGCGCGCGAGGTGGCGGCGGGCC
GTGGAGTGCGAATCGTCGAG

Downstream 100 bases:

>100_bases
CCCCTACCGAACTCGATCGACTGGTGGCGCAAATCGGAGAAGAGATTCTGGCCCGTGTGGGTCAGGGCGGCGGCGCGCCG
AAGAAGGGCGAAGGGCTGAA

Product: ribose-5-phosphate isomerase

Products: D-Ribulose 5-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MAEDQLTALAPPERTIAIGSDHGGFRLKEALKPLLEGLGLQVRDVGVNEEKPADYPDIAHAVAKLVAGGTAARGVIIDGA
GIGSCMAANKVPGIRAALCYDKASAKNGREHNDSNVLTLGARLLTQTQAEEVLRTWLETPFAGGRHQARVQKIMDVEQQY
SKKASS

Sequences:

>Translated_166_residues
MAEDQLTALAPPERTIAIGSDHGGFRLKEALKPLLEGLGLQVRDVGVNEEKPADYPDIAHAVAKLVAGGTAARGVIIDGA
GIGSCMAANKVPGIRAALCYDKASAKNGREHNDSNVLTLGARLLTQTQAEEVLRTWLETPFAGGRHQARVQKIMDVEQQY
SKKASS
>Mature_165_residues
AEDQLTALAPPERTIAIGSDHGGFRLKEALKPLLEGLGLQVRDVGVNEEKPADYPDIAHAVAKLVAGGTAARGVIIDGAG
IGSCMAANKVPGIRAALCYDKASAKNGREHNDSNVLTLGARLLTQTQAEEVLRTWLETPFAGGRHQARVQKIMDVEQQYS
KKASS

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=146, Percent_Identity=42.4657534246575, Blast_Score=117, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6 [C]

Molecular weight: Translated: 17612; Mature: 17481

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDQLTALAPPERTIAIGSDHGGFRLKEALKPLLEGLGLQVRDVGVNEEKPADYPDIAH
CCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHH
AVAKLVAGGTAARGVIIDGAGIGSCMAANKVPGIRAALCYDKASAKNGREHNDSNVLTLG
HHHHHHHCCCCCCCEEEECCCCCHHHHHCCCCCCHHHHHHCHHHCCCCCCCCCCCEEHHH
ARLLTQTQAEEVLRTWLETPFAGGRHQARVQKIMDVEQQYSKKASS
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEDQLTALAPPERTIAIGSDHGGFRLKEALKPLLEGLGLQVRDVGVNEEKPADYPDIAH
CCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHH
AVAKLVAGGTAARGVIIDGAGIGSCMAANKVPGIRAALCYDKASAKNGREHNDSNVLTLG
HHHHHHHCCCCCCCEEEECCCCCHHHHHCCCCCCHHHHHHCHHHCCCCCCCCCCCEEHHH
ARLLTQTQAEEVLRTWLETPFAGGRHQARVQKIMDVEQQYSKKASS
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: alpha-D-Ribose 5-phosphate [C]

Specific reaction: alpha-D-Ribose 5-phosphate <==> D-Ribulose 5-phosphate [C]

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]