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The map label for this gene is sacA [H]

Identifier: 116621198

GI number: 116621198

Start: 2642927

End: 2644423

Strand: Direct

Name: sacA [H]

Synonym: Acid_2079

Alternate gene names: 116621198

Gene position: 2642927-2644423 (Clockwise)

Preceding gene: 116621197

Following gene: 116621205

Centisome position: 26.52

GC content: 62.86

Gene sequence:

>1497_bases
ATGGATCGGCGCCGGTTTCTCCAAACCGCGGGTGCGGTGGGTGCATCGAAACGGATCCTGCAAGCCGCCGAAGCCGATCC
GGCTCTCGTCAAGGCGACCGAAGCCGTACTTGCCGCCGTGCCCACCGCGGAAGCGGATCCGGAGCGGCCGATTTACCACT
TTCGCCCTCCGGCAAACTGGACCAACGATCCCAACGGCACCATATACTACAAGGGCTGGCACCATCTCTTCTATCAGCTC
AACCCGTTCGTGGCGCGAATCGGGAGCCAGCACTGGGGACATGCCCGCAGCCGCGATCTGGTGAATTGGGAGCATCTGCC
GATCGCCATCTGGCCTTCCGAAGAGCGCGGCGAACGAGCTATCTTTTCCGGGAGCGCGGCGATCGCGGCCGATGGGCGTC
CGCGAATCCTCTACACCAGCATCGGCCAGGCGCAGCCCGAGCAGTGGCTGGCGATCCCAAAGGACGATGATCTCCTCAGC
TGGGAGAAGTTCCCCGGCAATCCCGTCCTCACCCAGGCCGCACACATAGCCGGTCCGATCAGCCAATGGCGCGATCCCTT
CCTGTTTACAGAAGGCGGCGCGACTTATATGGTCTGCGGCGGCGGGACCGCCGCGGGACGCGCCCAGGTGCAGTTGTACA
AGGCAGCCAAGCCCGATTTCACGGAATGGAGACACCTGGGCGCCGTCTTCCAGACTCTAGACCGGGAGAGCCGCAACTTC
GAGTGTCCCAACCTGTTCCCGCTCGCCGGCAAGTGGGTCATGATCGTATCTCCGAACCGGGTCTGCGAGTATTGGATCGG
CGATCTCGATATCGCGAAGGTACAGTTCACACCGCAGGCTCACGGCATTCTGGACGCGGGTGATGCCTACGCGAGCAACA
TCTCCGTGGATGACAAGGGACGCACGATCCTCTGGCTTTGGGGCCGCACCAACACCCCGCAGGGGAAGGGCTGGGGCAGC
GTGATGACCATGCCGCGAATCCTCTCGATTGGACCGGATGGGTATCTATTGCAGAGACCCGCTCCCGAATTCGAGAGCTT
GCGCGGAGCGGCCAGGAGCTTCCCCGCGAGGGGGCTCGAAACGCCATTCGTTCCAGAGGATGTCGCGGTGGATGCCGCAG
AGATTGAGGCCGAGTTCACCGGCACTGGCGTCTTCGGATTGGAACTGCGGCGGTCGTCCGCGGGGAAGCCGGGAGTGACG
GTTACGATCCAGGGCGGCTATGTGAACGTGGGAAACGCGCGCGCGTTCGTTGGCAATTCGGGACGCTACAAACTGCGCGT
TTTCCTCGATCGTCGTTGCCTTGAAGTGAATGTCAACGATGGCGTCGCCGCGGTCTACAACTGGTTCGACGCCGGACTGA
ACGATCTCGGGATTGCAGCCTTTGGCCGGGCCGGCAACGGGCGCGGCGGGATTGCCCCAGTACCGCCGCGCCTGGAATCG
CTAAGAGTTTGGCCCATGAAACCCGCGAAATTCAGCCTGGACCGTTTCCACGTCTAA

Upstream 100 bases:

>100_bases
ATATGCCGCCCCGCGACATCCTGGTGGACTTCCACCTGGTAGTGCGCGAATCCTGCGGCGCGCGGATGGCGCGGGATCGC
TCGTCGAGTTAGGATTCTGC

Downstream 100 bases:

>100_bases
ATCGTTAGTCGCCGGATGAGGCTTTATACTTAGGGCTGCTGACGCGCCACAGGTCATAGAACATCGACGTCATGTGGTAA
GGACTGAGTTCGGGTCCGTC

Product: glycosyl hydrolase family 32 protein

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MDRRRFLQTAGAVGASKRILQAAEADPALVKATEAVLAAVPTAEADPERPIYHFRPPANWTNDPNGTIYYKGWHHLFYQL
NPFVARIGSQHWGHARSRDLVNWEHLPIAIWPSEERGERAIFSGSAAIAADGRPRILYTSIGQAQPEQWLAIPKDDDLLS
WEKFPGNPVLTQAAHIAGPISQWRDPFLFTEGGATYMVCGGGTAAGRAQVQLYKAAKPDFTEWRHLGAVFQTLDRESRNF
ECPNLFPLAGKWVMIVSPNRVCEYWIGDLDIAKVQFTPQAHGILDAGDAYASNISVDDKGRTILWLWGRTNTPQGKGWGS
VMTMPRILSIGPDGYLLQRPAPEFESLRGAARSFPARGLETPFVPEDVAVDAAEIEAEFTGTGVFGLELRRSSAGKPGVT
VTIQGGYVNVGNARAFVGNSGRYKLRVFLDRRCLEVNVNDGVAAVYNWFDAGLNDLGIAAFGRAGNGRGGIAPVPPRLES
LRVWPMKPAKFSLDRFHV

Sequences:

>Translated_498_residues
MDRRRFLQTAGAVGASKRILQAAEADPALVKATEAVLAAVPTAEADPERPIYHFRPPANWTNDPNGTIYYKGWHHLFYQL
NPFVARIGSQHWGHARSRDLVNWEHLPIAIWPSEERGERAIFSGSAAIAADGRPRILYTSIGQAQPEQWLAIPKDDDLLS
WEKFPGNPVLTQAAHIAGPISQWRDPFLFTEGGATYMVCGGGTAAGRAQVQLYKAAKPDFTEWRHLGAVFQTLDRESRNF
ECPNLFPLAGKWVMIVSPNRVCEYWIGDLDIAKVQFTPQAHGILDAGDAYASNISVDDKGRTILWLWGRTNTPQGKGWGS
VMTMPRILSIGPDGYLLQRPAPEFESLRGAARSFPARGLETPFVPEDVAVDAAEIEAEFTGTGVFGLELRRSSAGKPGVT
VTIQGGYVNVGNARAFVGNSGRYKLRVFLDRRCLEVNVNDGVAAVYNWFDAGLNDLGIAAFGRAGNGRGGIAPVPPRLES
LRVWPMKPAKFSLDRFHV
>Mature_498_residues
MDRRRFLQTAGAVGASKRILQAAEADPALVKATEAVLAAVPTAEADPERPIYHFRPPANWTNDPNGTIYYKGWHHLFYQL
NPFVARIGSQHWGHARSRDLVNWEHLPIAIWPSEERGERAIFSGSAAIAADGRPRILYTSIGQAQPEQWLAIPKDDDLLS
WEKFPGNPVLTQAAHIAGPISQWRDPFLFTEGGATYMVCGGGTAAGRAQVQLYKAAKPDFTEWRHLGAVFQTLDRESRNF
ECPNLFPLAGKWVMIVSPNRVCEYWIGDLDIAKVQFTPQAHGILDAGDAYASNISVDDKGRTILWLWGRTNTPQGKGWGS
VMTMPRILSIGPDGYLLQRPAPEFESLRGAARSFPARGLETPFVPEDVAVDAAEIEAEFTGTGVFGLELRRSSAGKPGVT
VTIQGGYVNVGNARAFVGNSGRYKLRVFLDRRCLEVNVNDGVAAVYNWFDAGLNDLGIAAFGRAGNGRGGIAPVPPRLES
LRVWPMKPAKFSLDRFHV

Specific function: Unknown

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=288, Percent_Identity=28.8194444444444, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 54730; Mature: 54730

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRRRFLQTAGAVGASKRILQAAEADPALVKATEAVLAAVPTAEADPERPIYHFRPPANW
CCHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCCCCEEEECCCCCC
TNDPNGTIYYKGWHHLFYQLNPFVARIGSQHWGHARSRDLVNWEHLPIAIWPSEERGERA
CCCCCCEEEECCCEEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEE
IFSGSAAIAADGRPRILYTSIGQAQPEQWLAIPKDDDLLSWEKFPGNPVLTQAAHIAGPI
EEECCEEEEECCCCEEEEEECCCCCCHHEEECCCCCCCCCHHCCCCCCHHHHHHHHHCCH
SQWRDPFLFTEGGATYMVCGGGTAAGRAQVQLYKAAKPDFTEWRHLGAVFQTLDRESRNF
HHHCCCEEEECCCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
ECPNLFPLAGKWVMIVSPNRVCEYWIGDLDIAKVQFTPQAHGILDAGDAYASNISVDDKG
CCCCEEECCCCEEEEECCCCEEHHCCCCEEEEEEEECCCCCCCEECCCCHHCCCEECCCC
RTILWLWGRTNTPQGKGWGSVMTMPRILSIGPDGYLLQRPAPEFESLRGAARSFPARGLE
CEEEEEECCCCCCCCCCCCHHHCCCEEEEECCCCEEECCCCCHHHHHHHHHHCCCCCCCC
TPFVPEDVAVDAAEIEAEFTGTGVFGLELRRSSAGKPGVTVTIQGGYVNVGNARAFVGNS
CCCCCCHHCCCHHHEEEEECCCEEEEEEEECCCCCCCCEEEEEECCEEEECCCEEEECCC
GRYKLRVFLDRRCLEVNVNDGVAAVYNWFDAGLNDLGIAAFGRAGNGRGGIAPVPPRLES
CCEEEEEEEECEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
LRVWPMKPAKFSLDRFHV
EEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MDRRRFLQTAGAVGASKRILQAAEADPALVKATEAVLAAVPTAEADPERPIYHFRPPANW
CCHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCCCCEEEECCCCCC
TNDPNGTIYYKGWHHLFYQLNPFVARIGSQHWGHARSRDLVNWEHLPIAIWPSEERGERA
CCCCCCEEEECCCEEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEE
IFSGSAAIAADGRPRILYTSIGQAQPEQWLAIPKDDDLLSWEKFPGNPVLTQAAHIAGPI
EEECCEEEEECCCCEEEEEECCCCCCHHEEECCCCCCCCCHHCCCCCCHHHHHHHHHCCH
SQWRDPFLFTEGGATYMVCGGGTAAGRAQVQLYKAAKPDFTEWRHLGAVFQTLDRESRNF
HHHCCCEEEECCCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
ECPNLFPLAGKWVMIVSPNRVCEYWIGDLDIAKVQFTPQAHGILDAGDAYASNISVDDKG
CCCCEEECCCCEEEEECCCCEEHHCCCCEEEEEEEECCCCCCCEECCCCHHCCCEECCCC
RTILWLWGRTNTPQGKGWGSVMTMPRILSIGPDGYLLQRPAPEFESLRGAARSFPARGLE
CEEEEEECCCCCCCCCCCCHHHCCCEEEEECCCCEEECCCCCHHHHHHHHHHCCCCCCCC
TPFVPEDVAVDAAEIEAEFTGTGVFGLELRRSSAGKPGVTVTIQGGYVNVGNARAFVGNS
CCCCCCHHCCCHHHEEEEECCCEEEEEEEECCCCCCCCEEEEEECCEEEECCCEEEECCC
GRYKLRVFLDRRCLEVNVNDGVAAVYNWFDAGLNDLGIAAFGRAGNGRGGIAPVPPRLES
CCEEEEEEEECEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
LRVWPMKPAKFSLDRFHV
EEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3100393; 7934828; 9384377; 3122206 [H]