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The map label for this gene is rho [H]

Identifier: 116621148

GI number: 116621148

Start: 2569775

End: 2570998

Strand: Direct

Name: rho [H]

Synonym: Acid_2029

Alternate gene names: 116621148

Gene position: 2569775-2570998 (Clockwise)

Preceding gene: 116621147

Following gene: 116621149

Centisome position: 25.79

GC content: 60.7

Gene sequence:

>1224_bases
ATGAGCATTCAGAAGCTGAATGACGTGGCCAAGGGAATGAATATCGCCGGCGCCGCCGGACTTCGCAAACAGGAACTGAT
CTTCAAGATCCTGCAGACCCAGGCCGAGAAGAGCGGCCTGATCTTCAGCGAAGGCGTGCTCGAATGCCTGCCCGATGGCT
TCGGCTTCCTGCGCGCGCCCGAGTACAACTACCTTCCCGGCCCCGACGACGTCTATGTTTCGCCCAGCCAGATCCGCCGT
TTCGACCTGCGCACCGGCGACACCATCAGCGGCCAGATCCGCCCGCCCAAGGAAGGCGAGCGCTACTTCGCGCTGATCAA
AGTAGACGCTATCAACTTCGAACCGCCCGAGGAAGCGCGCAACAAGATCTTCTTCGACAACCTGACGCCGCTGTATCCCA
ACGAGCGGCTCAAGCTCGAGACCGCGAAGGACAACTATTCGGGCCGCGTGATGGACTTGCTGACTCCCATCGGCAAGGGA
CAGCGCGGACTGATCGTTTCCCCTCCGCGCACCGGTAAGACGATGCTGCTGCAAAGCATCGCGAACTCGGTGACCACCAA
TCATCCCGAAGTCACGCTGATCGTACTCCTGATCGACGAGCGTCCCGAGGAAGTCACCGATATGCAGCGCAGCGTGCGCG
GCGAAGTCATCAGTTCCACCTTCGACGAGCCCGCAACGCGCCACGTACAGGTGGCCGAGATGGTGATTGAGAAGGCCAAG
CGCCTTGTCGAACACAAGCGCGACGTCGTAATCCTGCTGGATTCCATCACGCGCCTGGCGCGCGCCTACAACACCATCGT
CCCGCCGAGCGGCAAGGTGCTCTCGGGCGGCGTGGATTCGAATGCGCTGCAGCGTCCGAAGCGGTTTTTCGGCGCGGCGC
GCAACATCGAAGAGGGCGGCTCGCTGACCATCATCGCGACCGCGCTGATCGATACCGGCAGCCGCATGGACGACGTGATT
TTCGAAGAGTTCAAGGGCACCGGCAACATGGAAATCCACCTGGAACGCAAACTGGTGGACAAGCGCGTGTTCCCGGCGAT
CGATATCAACAAGAGCGGCACGCGTAAGGAAGAGCTGCTGCTGCCCAAGGACGAACTCAATCGCGTGTGGATCCTGCGCA
AGGTGCTGAACCCCTTGTCGCCTGTCGAGACTATGGAGTTACTGCTCGATAAGCTCAGCAAGACCATGAGCAACGCGCAG
TTCCTCGGCTCGATGAGCGGCTAG

Upstream 100 bases:

>100_bases
CTCGCTCGAAAGCCATCCGGGAGCGCAGCGCCGCAAGCAGGGTTCGGCGCAGGGCGCCAAGACCGGTACCCCGACACTCG
ACCTCGTCGAGTTGAAGGAC

Downstream 100 bases:

>100_bases
AGCGGCGCATATTTCGAAAATCGACGGCGGGTGGCTTCGGCTGCCCGCCGTTTTCTTTTTCGGGGGTATCATATTCCCAT
GCTGACCCGACGAAACTTTG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSIQKLNDVAKGMNIAGAAGLRKQELIFKILQTQAEKSGLIFSEGVLECLPDGFGFLRAPEYNYLPGPDDVYVSPSQIRR
FDLRTGDTISGQIRPPKEGERYFALIKVDAINFEPPEEARNKIFFDNLTPLYPNERLKLETAKDNYSGRVMDLLTPIGKG
QRGLIVSPPRTGKTMLLQSIANSVTTNHPEVTLIVLLIDERPEEVTDMQRSVRGEVISSTFDEPATRHVQVAEMVIEKAK
RLVEHKRDVVILLDSITRLARAYNTIVPPSGKVLSGGVDSNALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVI
FEEFKGTGNMEIHLERKLVDKRVFPAIDINKSGTRKEELLLPKDELNRVWILRKVLNPLSPVETMELLLDKLSKTMSNAQ
FLGSMSG

Sequences:

>Translated_407_residues
MSIQKLNDVAKGMNIAGAAGLRKQELIFKILQTQAEKSGLIFSEGVLECLPDGFGFLRAPEYNYLPGPDDVYVSPSQIRR
FDLRTGDTISGQIRPPKEGERYFALIKVDAINFEPPEEARNKIFFDNLTPLYPNERLKLETAKDNYSGRVMDLLTPIGKG
QRGLIVSPPRTGKTMLLQSIANSVTTNHPEVTLIVLLIDERPEEVTDMQRSVRGEVISSTFDEPATRHVQVAEMVIEKAK
RLVEHKRDVVILLDSITRLARAYNTIVPPSGKVLSGGVDSNALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVI
FEEFKGTGNMEIHLERKLVDKRVFPAIDINKSGTRKEELLLPKDELNRVWILRKVLNPLSPVETMELLLDKLSKTMSNAQ
FLGSMSG
>Mature_406_residues
SIQKLNDVAKGMNIAGAAGLRKQELIFKILQTQAEKSGLIFSEGVLECLPDGFGFLRAPEYNYLPGPDDVYVSPSQIRRF
DLRTGDTISGQIRPPKEGERYFALIKVDAINFEPPEEARNKIFFDNLTPLYPNERLKLETAKDNYSGRVMDLLTPIGKGQ
RGLIVSPPRTGKTMLLQSIANSVTTNHPEVTLIVLLIDERPEEVTDMQRSVRGEVISSTFDEPATRHVQVAEMVIEKAKR
LVEHKRDVVILLDSITRLARAYNTIVPPSGKVLSGGVDSNALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIF
EEFKGTGNMEIHLERKLVDKRVFPAIDINKSGTRKEELLLPKDELNRVWILRKVLNPLSPVETMELLLDKLSKTMSNAQF
LGSMSG

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=406, Percent_Identity=67.2413793103448, Blast_Score=584, Evalue=1e-168,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 45414; Mature: 45283

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQKLNDVAKGMNIAGAAGLRKQELIFKILQTQAEKSGLIFSEGVLECLPDGFGFLRAP
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCHHHHCCCCCCCCCCC
EYNYLPGPDDVYVSPSQIRRFDLRTGDTISGQIRPPKEGERYFALIKVDAINFEPPEEAR
CCCCCCCCCCEEECHHHHHEEECCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHH
NKIFFDNLTPLYPNERLKLETAKDNYSGRVMDLLTPIGKGQRGLIVSPPRTGKTMLLQSI
CEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHH
ANSVTTNHPEVTLIVLLIDERPEEVTDMQRSVRGEVISSTFDEPATRHVQVAEMVIEKAK
HHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RLVEHKRDVVILLDSITRLARAYNTIVPPSGKVLSGGVDSNALQRPKRFFGAARNIEEGG
HHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCC
SLTIIATALIDTGSRMDDVIFEEFKGTGNMEIHLERKLVDKRVFPAIDINKSGTRKEELL
CEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCEEECCCCCCCHHHCC
LPKDELNRVWILRKVLNPLSPVETMELLLDKLSKTMSNAQFLGSMSG
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SIQKLNDVAKGMNIAGAAGLRKQELIFKILQTQAEKSGLIFSEGVLECLPDGFGFLRAP
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCHHHHCCCCCCCCCCC
EYNYLPGPDDVYVSPSQIRRFDLRTGDTISGQIRPPKEGERYFALIKVDAINFEPPEEAR
CCCCCCCCCCEEECHHHHHEEECCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHH
NKIFFDNLTPLYPNERLKLETAKDNYSGRVMDLLTPIGKGQRGLIVSPPRTGKTMLLQSI
CEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHH
ANSVTTNHPEVTLIVLLIDERPEEVTDMQRSVRGEVISSTFDEPATRHVQVAEMVIEKAK
HHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RLVEHKRDVVILLDSITRLARAYNTIVPPSGKVLSGGVDSNALQRPKRFFGAARNIEEGG
HHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCC
SLTIIATALIDTGSRMDDVIFEEFKGTGNMEIHLERKLVDKRVFPAIDINKSGTRKEELL
CEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCEEECCCCCCCHHHCC
LPKDELNRVWILRKVLNPLSPVETMELLLDKLSKTMSNAQFLGSMSG
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]