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The map label for this gene is tig

Identifier: 116621144

GI number: 116621144

Start: 2563424

End: 2564692

Strand: Direct

Name: tig

Synonym: Acid_2025

Alternate gene names: 116621144

Gene position: 2563424-2564692 (Clockwise)

Preceding gene: 116621142

Following gene: 116621145

Centisome position: 25.72

GC content: 59.65

Gene sequence:

>1269_bases
TTGGCATTAGTAGAAGGTTGCAAGCACTCGCTCGAAATCTCCATCCCAGCCGCTGAAGTCGAGACCGAGACAAACCGCGT
CGTTTTGGACGTACAGAAGCGCGCCAAACTGCCGGGCTTCCGCCCGGGAAAGTCGCCCGCATCGATTATTCGCAAACAGT
TTGCCGGCGATATCCGGCAGCAGGTGTTGGAGAGTCTCATCCCGACTCACCTGCAGAAGCAGTTGGAGGCGGAGAACCTC
AACGTGGTCGGTACGCCCGATATTAGCGACGTGCACTTCCACGATAACGAACCGCTGCGCTTTAAGGCGACTTTCGAAGT
GGTACCGGAGATCGAACTCGGCGAGTACACCAACATCGAAGTCCCTTACCAGGACCCCGAAGTCACGGACGAGGACGTCA
CCAGCCGCATCGACGAGATCCGCGAGCAGAAAGCGCAGTACGTGAATATCGATCCGCGCCCGCTGGCCGATGGCGATTTC
GCCGTGGTTTCCCTCGAAAGCCTGGAAGGCGTCTCCGGCGACCCCGTCAAGACCGACGAGATGCAGTTGGAAATCGGCGC
CAAGGAGACCTTTGAGGCCTTCAACGAGAATCTCCGCGGCCTCTCGCCCGGCGACGAAAAGGATTTCGAAGTCGAGTACC
CGGCCGATTACGGCAGCGAAAAACTGGCCGGCCGCAAGGTGAAATTCCACGCGGTCGTCAAAGGGCTGCGCAAGAAGGAA
TTGCCGGAATTGGACGATGAGTTCGCCCAGGAGCTCGGCGATTACCGCACCGTGGACGAGTTGAAGGAAGCCGTCCGCAA
GGCGATCTTCTCCCAGCGCCAGTACGAGGCCCAGCAGGAAGCCAAGAATAAGATCGTCGATAAGCTGGTGGACGCCCACG
AATTTCCGGTGCCGGAAGTCTTCGTGGAACGCCAGATCCGTAACCGCGTGGAACAGAGCCTGCGCGCCATGGCCGAGCAG
GGCGTGGACCCGAGCAAGCTGAAACTGGATTGGGAAAAGGTGAAGGAGGCCCAGCGCGAAAAGGCGACGCGCGAGGTCAA
AGCCTCCCTCCTGCTGGGCAAGGTCAGCGATCGCGAAGCCATCCGCGCCACCCGCGATGAGGTGGATGCCGAAGTGGAGA
AGGCTGCGCGCCAGCAGCGCAAACCCGTAGCGGCGGTCCACATGGAATTCGAAAAAGACGGAACGCTGGGCAGAATCGCC
AGCCACATTCAAACCGACAAAACGTTGAGCTTCTTGTTCGAACGGGCGCGCAAGACGGCGGAATCGTAA

Upstream 100 bases:

>100_bases
GGAAGCCGCCATACCGGAGCACCAGCAAGCCGGTAGCCGGACCGAAAGCTCGTGTTATAATTTGGTTTAACCCTTACTCT
GTCCCCGAGGTCAAATCCAC

Downstream 100 bases:

>100_bases
ATCCGCCGTCGCGCGTCTAAATTGGTGTACGATCCGGAGAGCCGGCGTAACCCTTAACCAAAGTAACTGCACAGGACGCC
CCGCACCCTTCAGGCAACAT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MALVEGCKHSLEISIPAAEVETETNRVVLDVQKRAKLPGFRPGKSPASIIRKQFAGDIRQQVLESLIPTHLQKQLEAENL
NVVGTPDISDVHFHDNEPLRFKATFEVVPEIELGEYTNIEVPYQDPEVTDEDVTSRIDEIREQKAQYVNIDPRPLADGDF
AVVSLESLEGVSGDPVKTDEMQLEIGAKETFEAFNENLRGLSPGDEKDFEVEYPADYGSEKLAGRKVKFHAVVKGLRKKE
LPELDDEFAQELGDYRTVDELKEAVRKAIFSQRQYEAQQEAKNKIVDKLVDAHEFPVPEVFVERQIRNRVEQSLRAMAEQ
GVDPSKLKLDWEKVKEAQREKATREVKASLLLGKVSDREAIRATRDEVDAEVEKAARQQRKPVAAVHMEFEKDGTLGRIA
SHIQTDKTLSFLFERARKTAES

Sequences:

>Translated_422_residues
MALVEGCKHSLEISIPAAEVETETNRVVLDVQKRAKLPGFRPGKSPASIIRKQFAGDIRQQVLESLIPTHLQKQLEAENL
NVVGTPDISDVHFHDNEPLRFKATFEVVPEIELGEYTNIEVPYQDPEVTDEDVTSRIDEIREQKAQYVNIDPRPLADGDF
AVVSLESLEGVSGDPVKTDEMQLEIGAKETFEAFNENLRGLSPGDEKDFEVEYPADYGSEKLAGRKVKFHAVVKGLRKKE
LPELDDEFAQELGDYRTVDELKEAVRKAIFSQRQYEAQQEAKNKIVDKLVDAHEFPVPEVFVERQIRNRVEQSLRAMAEQ
GVDPSKLKLDWEKVKEAQREKATREVKASLLLGKVSDREAIRATRDEVDAEVEKAARQQRKPVAAVHMEFEKDGTLGRIA
SHIQTDKTLSFLFERARKTAES
>Mature_421_residues
ALVEGCKHSLEISIPAAEVETETNRVVLDVQKRAKLPGFRPGKSPASIIRKQFAGDIRQQVLESLIPTHLQKQLEAENLN
VVGTPDISDVHFHDNEPLRFKATFEVVPEIELGEYTNIEVPYQDPEVTDEDVTSRIDEIREQKAQYVNIDPRPLADGDFA
VVSLESLEGVSGDPVKTDEMQLEIGAKETFEAFNENLRGLSPGDEKDFEVEYPADYGSEKLAGRKVKFHAVVKGLRKKEL
PELDDEFAQELGDYRTVDELKEAVRKAIFSQRQYEAQQEAKNKIVDKLVDAHEFPVPEVFVERQIRNRVEQSLRAMAEQG
VDPSKLKLDWEKVKEAQREKATREVKASLLLGKVSDREAIRATRDEVDAEVEKAARQQRKPVAAVHMEFEKDGTLGRIAS
HIQTDKTLSFLFERARKTAES

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=379, Percent_Identity=27.9683377308707, Blast_Score=142, Evalue=4e-35,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_SOLUE (Q026Q4)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_823300.1
- ProteinModelPortal:   Q026Q4
- SMR:   Q026Q4
- STRING:   Q026Q4
- GeneID:   4429771
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_2025
- NMPDR:   fig|234267.9.peg.1927
- eggNOG:   COG0544
- HOGENOM:   HBG290822
- OMA:   RFIPGFE
- PhylomeDB:   Q026Q4
- ProtClustDB:   CLSK774579
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 47770; Mature: 47639

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVEGCKHSLEISIPAAEVETETNRVVLDVQKRAKLPGFRPGKSPASIIRKQFAGDIRQ
CCCHHCCCCCEEEECCHHHHCCCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
QVLESLIPTHLQKQLEAENLNVVGTPDISDVHFHDNEPLRFKATFEVVPEIELGEYTNIE
HHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEECCCCCEEEEEEEEECCCCCCCCCCCCC
VPYQDPEVTDEDVTSRIDEIREQKAQYVNIDPRPLADGDFAVVSLESLEGVSGDPVKTDE
CCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEHHHCCCCCCCCCCCCC
MQLEIGAKETFEAFNENLRGLSPGDEKDFEVEYPADYGSEKLAGRKVKFHAVVKGLRKKE
EEEECCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCHHHCCCCCHHHHHHHHHHHHC
LPELDDEFAQELGDYRTVDELKEAVRKAIFSQRQYEAQQEAKNKIVDKLVDAHEFPVPEV
CCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FVERQIRNRVEQSLRAMAEQGVDPSKLKLDWEKVKEAQREKATREVKASLLLGKVSDREA
HHHHHHHHHHHHHHHHHHHCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IRATRDEVDAEVEKAARQQRKPVAAVHMEFEKDGTLGRIASHIQTDKTLSFLFERARKTA
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
ES
CC
>Mature Secondary Structure 
ALVEGCKHSLEISIPAAEVETETNRVVLDVQKRAKLPGFRPGKSPASIIRKQFAGDIRQ
CCHHCCCCCEEEECCHHHHCCCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
QVLESLIPTHLQKQLEAENLNVVGTPDISDVHFHDNEPLRFKATFEVVPEIELGEYTNIE
HHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEECCCCCEEEEEEEEECCCCCCCCCCCCC
VPYQDPEVTDEDVTSRIDEIREQKAQYVNIDPRPLADGDFAVVSLESLEGVSGDPVKTDE
CCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEHHHCCCCCCCCCCCCC
MQLEIGAKETFEAFNENLRGLSPGDEKDFEVEYPADYGSEKLAGRKVKFHAVVKGLRKKE
EEEECCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCHHHCCCCCHHHHHHHHHHHHC
LPELDDEFAQELGDYRTVDELKEAVRKAIFSQRQYEAQQEAKNKIVDKLVDAHEFPVPEV
CCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FVERQIRNRVEQSLRAMAEQGVDPSKLKLDWEKVKEAQREKATREVKASLLLGKVSDREA
HHHHHHHHHHHHHHHHHHHCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IRATRDEVDAEVEKAARQQRKPVAAVHMEFEKDGTLGRIASHIQTDKTLSFLFERARKTA
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
ES
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA