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The map label for this gene is purA

Identifier: 116621141

GI number: 116621141

Start: 2557219

End: 2558523

Strand: Direct

Name: purA

Synonym: Acid_2022

Alternate gene names: 116621141

Gene position: 2557219-2558523 (Clockwise)

Preceding gene: 116621135

Following gene: 116621142

Centisome position: 25.66

GC content: 62.38

Gene sequence:

>1305_bases
ATGGGAAATGTAATCATCCTCGGCTCTCAGTGGGGCGATGAGGGTAAAGGCAAAATCGTCGATCTCTTCGCCGAAAGGTT
CGATATCGTCGCGCGCTATCAAGGCGGACACAACGCGGGCCACACGGTCTTCATTGGCGAAAAAAAATTCGTCCTCAAGC
TGATTCCGAGCGGCATTCTCCGCCCCGGAAAGAAAGCTGTCATCGGTAATGGCCTGGTGATCGACCCCGCGGCGCTGCTC
AGCGAGATCGATACTCTTCAGGCGGCCGGCGTGCCCGTGATGGGCAACCTGTTCATCAGCAATCGCGCCCATGTCCTGTT
TCCAGCGCACCGCATGATGGAAAAAATGTCCGAGGGTCGCGAAGGCCGCGTCTCCATCGGCACCACCTCGCGCGGCATCG
GTCCCTGCTATGAGGACAAAATCGCGCGCCGCGGCATCCGCATCGCCGACCTGCTGGACACGGAGTTTTTCCGCGCGCAA
TACGCTTCCGTGATGGAAGAAAAGGTCTTGATCGCCAAAGCGCTGGGAATTTACAGCGAGCTCGATCTACGCGCCATTCG
CGACGAGTACGAAGCCTTCGCCGAACGCATCCGGCCGATGGTGTGCGACACCTCGGTGCTGCTCAACGACGCGATCCGCT
CCGGCAAGACCGTGATGTTCGAGGGCGCCCAGGGCACCATGCTCGATATCGACCACGGGACCTACCCCTTCGTGACCTCC
TCCAGCGCCAGCGCCGGCGGAGCCTGCACCGGCACCGGCGTGGCCCCGACCCGCATCAGCGGCGTGCTCGGAGTTTCCAA
AGCCTACATCACCCGCGTCGGCGGCGGGCCCTTCCCCACCGAGGCCTTCGACGGCGCGGGCGACCGCATTCGCGAGCGCG
GCAAGGAATTCGGTGCGGTCACCGGCCGGCCCCGGCGCTGCGGATGGTTCGACGTGCCGCTCTTGCGCTACACCGCCAAC
ATCAACGGCTTCGATAGCCTCGTAATTACCAAACTCGATGTGCTCGACGAATTCGACCAGATTCCTGTCTGCGTCTCCTA
CCGGATCGGAAACCGCGAAGTTGTCGATATGCCGCCGACCGTCGCCGAGATGGAAAAAGTCGAGCCGGTCTACGAGTGCG
TGCCGGGCTGGAACACCTCGACGTTCGGAATATCCCAATTCGGTGAGCTCCCGGCCAAGGCCAAGGAATACCTGGCCTAC
CTCGAGAACCGCACTGGCGTGGAAGTGGGCTGCGTCTCTACCGGCCCGGAGCGCAACCAGACCATCGTCCGCGCCGGCTC
CCGCTTCGAATCCCTGATCGGGTAA

Upstream 100 bases:

>100_bases
CGTATCATGCGCGGGGCATTTCCTGGGAGCAGTAGGTCCGCTTTTCCGTTTCGGGTCACTTTACCTCCCGACTGCGGTAG
ACTAGTTAGGTTTGGTCAAA

Downstream 100 bases:

>100_bases
AACCAACTAACCGCGACCGCAAGGGAACGAGCTCGATCTGCCTTGGCACTCCGCCTGCTCTTTCGCCCCCGGTAGGCATC
GAAAATCATGAATATTCGGC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MGNVIILGSQWGDEGKGKIVDLFAERFDIVARYQGGHNAGHTVFIGEKKFVLKLIPSGILRPGKKAVIGNGLVIDPAALL
SEIDTLQAAGVPVMGNLFISNRAHVLFPAHRMMEKMSEGREGRVSIGTTSRGIGPCYEDKIARRGIRIADLLDTEFFRAQ
YASVMEEKVLIAKALGIYSELDLRAIRDEYEAFAERIRPMVCDTSVLLNDAIRSGKTVMFEGAQGTMLDIDHGTYPFVTS
SSASAGGACTGTGVAPTRISGVLGVSKAYITRVGGGPFPTEAFDGAGDRIRERGKEFGAVTGRPRRCGWFDVPLLRYTAN
INGFDSLVITKLDVLDEFDQIPVCVSYRIGNREVVDMPPTVAEMEKVEPVYECVPGWNTSTFGISQFGELPAKAKEYLAY
LENRTGVEVGCVSTGPERNQTIVRAGSRFESLIG

Sequences:

>Translated_434_residues
MGNVIILGSQWGDEGKGKIVDLFAERFDIVARYQGGHNAGHTVFIGEKKFVLKLIPSGILRPGKKAVIGNGLVIDPAALL
SEIDTLQAAGVPVMGNLFISNRAHVLFPAHRMMEKMSEGREGRVSIGTTSRGIGPCYEDKIARRGIRIADLLDTEFFRAQ
YASVMEEKVLIAKALGIYSELDLRAIRDEYEAFAERIRPMVCDTSVLLNDAIRSGKTVMFEGAQGTMLDIDHGTYPFVTS
SSASAGGACTGTGVAPTRISGVLGVSKAYITRVGGGPFPTEAFDGAGDRIRERGKEFGAVTGRPRRCGWFDVPLLRYTAN
INGFDSLVITKLDVLDEFDQIPVCVSYRIGNREVVDMPPTVAEMEKVEPVYECVPGWNTSTFGISQFGELPAKAKEYLAY
LENRTGVEVGCVSTGPERNQTIVRAGSRFESLIG
>Mature_433_residues
GNVIILGSQWGDEGKGKIVDLFAERFDIVARYQGGHNAGHTVFIGEKKFVLKLIPSGILRPGKKAVIGNGLVIDPAALLS
EIDTLQAAGVPVMGNLFISNRAHVLFPAHRMMEKMSEGREGRVSIGTTSRGIGPCYEDKIARRGIRIADLLDTEFFRAQY
ASVMEEKVLIAKALGIYSELDLRAIRDEYEAFAERIRPMVCDTSVLLNDAIRSGKTVMFEGAQGTMLDIDHGTYPFVTSS
SASAGGACTGTGVAPTRISGVLGVSKAYITRVGGGPFPTEAFDGAGDRIRERGKEFGAVTGRPRRCGWFDVPLLRYTANI
NGFDSLVITKLDVLDEFDQIPVCVSYRIGNREVVDMPPTVAEMEKVEPVYECVPGWNTSTFGISQFGELPAKAKEYLAYL
ENRTGVEVGCVSTGPERNQTIVRAGSRFESLIG

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=425, Percent_Identity=42.8235294117647, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI34577063, Length=429, Percent_Identity=42.1911421911422, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=41.1616161616162, Blast_Score=302, Evalue=4e-82,
Organism=Escherichia coli, GI1790620, Length=422, Percent_Identity=48.3412322274881, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25146553, Length=428, Percent_Identity=42.5233644859813, Blast_Score=337, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI25146555, Length=428, Percent_Identity=42.5233644859813, Blast_Score=336, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=43.0232558139535, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=45.1306413301663, Blast_Score=372, Evalue=1e-103,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_SOLUE (Q026Q7)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_823297.1
- ProteinModelPortal:   Q026Q7
- SMR:   Q026Q7
- STRING:   Q026Q7
- GeneID:   4429803
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_2022
- NMPDR:   fig|234267.9.peg.1924
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- PhylomeDB:   Q026Q7
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47104; Mature: 46972

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 129-129 BINDING 143-143 BINDING 224-224 BINDING 239-239 BINDING 303-303 BINDING 305-305

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNVIILGSQWGDEGKGKIVDLFAERFDIVARYQGGHNAGHTVFIGEKKFVLKLIPSGIL
CCCEEEEECCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCCEEEEECCHHHC
RPGKKAVIGNGLVIDPAALLSEIDTLQAAGVPVMGNLFISNRAHVLFPAHRMMEKMSEGR
CCCCCEEECCCEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECHHHHHHHHHCCC
EGRVSIGTTSRGIGPCYEDKIARRGIRIADLLDTEFFRAQYASVMEEKVLIAKALGIYSE
CCEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDLRAIRDEYEAFAERIRPMVCDTSVLLNDAIRSGKTVMFEGAQGTMLDIDHGTYPFVTS
HHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEEC
SSASAGGACTGTGVAPTRISGVLGVSKAYITRVGGGPFPTEAFDGAGDRIRERGKEFGAV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCHHCCCCCHHHHHHHHHCCCC
TGRPRRCGWFDVPLLRYTANINGFDSLVITKLDVLDEFDQIPVCVSYRIGNREVVDMPPT
CCCCCCCCEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCC
VAEMEKVEPVYECVPGWNTSTFGISQFGELPAKAKEYLAYLENRTGVEVGCVSTGPERNQ
HHHHHHCCHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCC
TIVRAGSRFESLIG
CEECCCHHHHHHCC
>Mature Secondary Structure 
GNVIILGSQWGDEGKGKIVDLFAERFDIVARYQGGHNAGHTVFIGEKKFVLKLIPSGIL
CCEEEEECCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCCEEEEECCHHHC
RPGKKAVIGNGLVIDPAALLSEIDTLQAAGVPVMGNLFISNRAHVLFPAHRMMEKMSEGR
CCCCCEEECCCEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECHHHHHHHHHCCC
EGRVSIGTTSRGIGPCYEDKIARRGIRIADLLDTEFFRAQYASVMEEKVLIAKALGIYSE
CCEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDLRAIRDEYEAFAERIRPMVCDTSVLLNDAIRSGKTVMFEGAQGTMLDIDHGTYPFVTS
HHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEEC
SSASAGGACTGTGVAPTRISGVLGVSKAYITRVGGGPFPTEAFDGAGDRIRERGKEFGAV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCHHCCCCCHHHHHHHHHCCCC
TGRPRRCGWFDVPLLRYTANINGFDSLVITKLDVLDEFDQIPVCVSYRIGNREVVDMPPT
CCCCCCCCEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCC
VAEMEKVEPVYECVPGWNTSTFGISQFGELPAKAKEYLAYLENRTGVEVGCVSTGPERNQ
HHHHHHCCHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCC
TIVRAGSRFESLIG
CEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA