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The map label for this gene is dgoD [H]

Identifier: 116621006

GI number: 116621006

Start: 2345097

End: 2346380

Strand: Direct

Name: dgoD [H]

Synonym: Acid_1887

Alternate gene names: 116621006

Gene position: 2345097-2346380 (Clockwise)

Preceding gene: 116621005

Following gene: 116621007

Centisome position: 23.53

GC content: 63.08

Gene sequence:

>1284_bases
ATGGCGAATTGGACTCGAAGAGGTTTTCTTGGATCGCTTCCGGCGGCGGCCGTTACCTCGCTGGCGGCACAGCAGCGCGG
GCGCGCGCCGATCAAGATCACGGATCTGCGATGTGCGGTGATCGGACGAAACCCGGTAGTCCGCATCGTCACGGATCAGG
GCATCTCGGGCTACGGACAGGCCGAGTCCGCGAAACCGTATCTCAAGCCGATGGTCCTGTTCTACAAGGACTATCTTCTC
GGTGAAGACCCGACGGACGTAAACCGGGTCATGATGAAGATCCGGCGGATGGGCGCATTCAAACCGTGGGGCTCGGCGGT
GAGCGCGATCGAAATCGCGCTGTGGGATATCGCGGGACAGGCGGCCGGAGTGCCCGTATACAAGTTGCTGGGCGGCAAGA
TCCGGAGCCGCGTCCGCGCCTACAACGGCGGCGTACGCTTCCCGATGACCGGCCAATCGCCGCAGGACTACGCGGAGAAC
GCGGCCAAGATGAAGGAAGCCAAGGAAGGCTTCACGCTGATCAAGCAGGCGGTCGGCTTTCACAATCCCGCGATGATGCG
CGCCACGCCCAACGGCTGGTACGACGAGCCGCGCACGGGACCGCCGCACTCGGACCGGGGCCTGCTGACCGAGCGCGGCC
TCAAAGAGATCATCGCGCGCGTCGAGGCCATAAAGAAGGTCCTCGGGGATGAGATCGGCCTGGCGCTGGACTGCGGTCCG
GGGTGGACGGTAAAGGACGCGATCACGTTTGCGCGGGCATTGGAACCGCTCCACATCACGTGGCTCGAAGACATCATCAC
CGGCGACTATTCGCCGTATCCCAACGCCGAAGTCTTCCGGGAGGTGACGCAAAGCACTTCGCTGCCGATTCACACCGGCG
AGCAGATTTATCTGCGGGAGAACTTCAAGGATCTGATCGAGAAGCAGGCTGTCAACGTGATCGGACCGGATCCGGAGGAC
GTCGGTGGACTGGCGGAGTTGAAGTGGATCGCGGAGTACGCCGACCTGCACGGCATCCTGGTGGCTCCGCACGGGATCTT
CGACGGCTTGATCGGGCTGGCCGCGCATGTTCATCTGGCGGCCGCACTCCCGCAAAACTATATCGCGTTCGAATATCCGG
TGGGCCAGCCGGAATGGTGGTACGACATCGTCGATGGCTTGCCGAATCCGATCATGAAGCAAGGCTTCATCGACGTGTGG
GACCGGCCGGGGCTCGGTGTCACGTTCCGCGTGCCAGCCGCGAAGGCGCGGCTGGCGGAAGAGGATCGCGGCTTCTTCGA
CTAG

Upstream 100 bases:

>100_bases
GGGCATCTGGTTAGCGTTCCTTAAGAACGTGCGGCTTGCGCCACGAAACGTGGGGCAAGCCATACGAATCCGGAGGGTGT
AGACTGGGTTCGATTCAGCC

Downstream 100 bases:

>100_bases
GGCGTCGCACTGCAGCATATGTTGCGCGCGCTTGCGATCTTTGGGGCCGGCTTTCTACTGCAGGCGCCGCCCTCCGCGAA
CGGACAGGCGTTCGATGTCG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MANWTRRGFLGSLPAAAVTSLAAQQRGRAPIKITDLRCAVIGRNPVVRIVTDQGISGYGQAESAKPYLKPMVLFYKDYLL
GEDPTDVNRVMMKIRRMGAFKPWGSAVSAIEIALWDIAGQAAGVPVYKLLGGKIRSRVRAYNGGVRFPMTGQSPQDYAEN
AAKMKEAKEGFTLIKQAVGFHNPAMMRATPNGWYDEPRTGPPHSDRGLLTERGLKEIIARVEAIKKVLGDEIGLALDCGP
GWTVKDAITFARALEPLHITWLEDIITGDYSPYPNAEVFREVTQSTSLPIHTGEQIYLRENFKDLIEKQAVNVIGPDPED
VGGLAELKWIAEYADLHGILVAPHGIFDGLIGLAAHVHLAAALPQNYIAFEYPVGQPEWWYDIVDGLPNPIMKQGFIDVW
DRPGLGVTFRVPAAKARLAEEDRGFFD

Sequences:

>Translated_427_residues
MANWTRRGFLGSLPAAAVTSLAAQQRGRAPIKITDLRCAVIGRNPVVRIVTDQGISGYGQAESAKPYLKPMVLFYKDYLL
GEDPTDVNRVMMKIRRMGAFKPWGSAVSAIEIALWDIAGQAAGVPVYKLLGGKIRSRVRAYNGGVRFPMTGQSPQDYAEN
AAKMKEAKEGFTLIKQAVGFHNPAMMRATPNGWYDEPRTGPPHSDRGLLTERGLKEIIARVEAIKKVLGDEIGLALDCGP
GWTVKDAITFARALEPLHITWLEDIITGDYSPYPNAEVFREVTQSTSLPIHTGEQIYLRENFKDLIEKQAVNVIGPDPED
VGGLAELKWIAEYADLHGILVAPHGIFDGLIGLAAHVHLAAALPQNYIAFEYPVGQPEWWYDIVDGLPNPIMKQGFIDVW
DRPGLGVTFRVPAAKARLAEEDRGFFD
>Mature_426_residues
ANWTRRGFLGSLPAAAVTSLAAQQRGRAPIKITDLRCAVIGRNPVVRIVTDQGISGYGQAESAKPYLKPMVLFYKDYLLG
EDPTDVNRVMMKIRRMGAFKPWGSAVSAIEIALWDIAGQAAGVPVYKLLGGKIRSRVRAYNGGVRFPMTGQSPQDYAENA
AKMKEAKEGFTLIKQAVGFHNPAMMRATPNGWYDEPRTGPPHSDRGLLTERGLKEIIARVEAIKKVLGDEIGLALDCGPG
WTVKDAITFARALEPLHITWLEDIITGDYSPYPNAEVFREVTQSTSLPIHTGEQIYLRENFKDLIEKQAVNVIGPDPEDV
GGLAELKWIAEYADLHGILVAPHGIFDGLIGLAAHVHLAAALPQNYIAFEYPVGQPEWWYDIVDGLPNPIMKQGFIDVWD
RPGLGVTFRVPAAKARLAEEDRGFFD

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=371, Percent_Identity=25.6064690026954, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1787864, Length=386, Percent_Identity=29.5336787564767, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI226510960, Length=311, Percent_Identity=27.0096463022508, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 47031; Mature: 46899

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANWTRRGFLGSLPAAAVTSLAAQQRGRAPIKITDLRCAVIGRNPVVRIVTDQGISGYGQ
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCEEEEEECCCCCCCCC
AESAKPYLKPMVLFYKDYLLGEDPTDVNRVMMKIRRMGAFKPWGSAVSAIEIALWDIAGQ
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
AAGVPVYKLLGGKIRSRVRAYNGGVRFPMTGQSPQDYAENAAKMKEAKEGFTLIKQAVGF
CCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
HNPAMMRATPNGWYDEPRTGPPHSDRGLLTERGLKEIIARVEAIKKVLGDEIGLALDCGP
CCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
GWTVKDAITFARALEPLHITWLEDIITGDYSPYPNAEVFREVTQSTSLPIHTGEQIYLRE
CCCHHHHHHHHHHCCCEEHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEHH
NFKDLIEKQAVNVIGPDPEDVGGLAELKWIAEYADLHGILVAPHGIFDGLIGLAAHVHLA
HHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHH
AALPQNYIAFEYPVGQPEWWYDIVDGLPNPIMKQGFIDVWDRPGLGVTFRVPAAKARLAE
HHCCCCCEEEECCCCCCHHHHHHHHCCCCHHHHCCCHHHCCCCCCCEEEECCHHHHHHHH
EDRGFFD
HCCCCCC
>Mature Secondary Structure 
ANWTRRGFLGSLPAAAVTSLAAQQRGRAPIKITDLRCAVIGRNPVVRIVTDQGISGYGQ
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCEEEEEECCCCCCCCC
AESAKPYLKPMVLFYKDYLLGEDPTDVNRVMMKIRRMGAFKPWGSAVSAIEIALWDIAGQ
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
AAGVPVYKLLGGKIRSRVRAYNGGVRFPMTGQSPQDYAENAAKMKEAKEGFTLIKQAVGF
CCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
HNPAMMRATPNGWYDEPRTGPPHSDRGLLTERGLKEIIARVEAIKKVLGDEIGLALDCGP
CCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
GWTVKDAITFARALEPLHITWLEDIITGDYSPYPNAEVFREVTQSTSLPIHTGEQIYLRE
CCCHHHHHHHHHHCCCEEHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEHH
NFKDLIEKQAVNVIGPDPEDVGGLAELKWIAEYADLHGILVAPHGIFDGLIGLAAHVHLA
HHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHH
AALPQNYIAFEYPVGQPEWWYDIVDGLPNPIMKQGFIDVWDRPGLGVTFRVPAAKARLAE
HHCCCCCEEEECCCCCCHHHHHHHHCCCCHHHHCCCHHHCCCCCCCEEEECCHHHHHHHH
EDRGFFD
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA