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The map label for this gene is mazG [H]

Identifier: 116620964

GI number: 116620964

Start: 2297027

End: 2297818

Strand: Direct

Name: mazG [H]

Synonym: Acid_1845

Alternate gene names: 116620964

Gene position: 2297027-2297818 (Clockwise)

Preceding gene: 116620961

Following gene: 116620965

Centisome position: 23.05

GC content: 63.01

Gene sequence:

>792_bases
ATGAGTACTGAAAACGCCGGCGAGAAGTTTCAAAAACTGGTCGCGATCATGGCCAGGCTCCGCGCGCCCGATGGCTGCCC
GTGGGATCGCGAGCAGACCTTCGACACGATCAAGCCCTATACGCTCGAAGAAACCTACGAAGTGCTGGACGCGATCGATC
GCCGCCAGTGGGACGAGCTTGCCGAAGAGCTCGGCGATTTCATGCTGCAGGCCGTGTTCTACGCGCAGATGGCCGCCGAG
CAGAAACTCTTCCGCATAGACGACGCGCTCGACGCGATTAACGAGAAACTGGTGCGGCGCCATCCCCATGTCTTCGGCGA
GGAATCCGCCGCGTCGGCTGACGACGTGATGGCCATCTGGGGACGCGTCAAAGGCTCGGAGCAGAAGAAGAAGGACAAGC
CCGCCGGCCTGCTGGGCGGCGTGCCGCGCACCCTTCCCGCGCTGGTCGAAGCGCAGCAGATCGCGTCCCGCGCCGCCGGC
GTGGGCTTCGATTGGGAGAACCCCGAGCAGGTGCTCGAGAAACTTCACGAGGAGCTCGCCGAGTTTGCGGTGGCGCGCAC
CAACGCGCAGCACGATGAGATCGAAGACGAGCTGGGCGATATGCTGTTCGTGATGGTCAACCTGGCGCGCTTCGTCAAGG
TGGATCCCGAACAAGCGCTGCGGCGCACCAACGCCAAATTTCGCAAGCGCTGGAGCCACATCGAAGCCAGGCTCGCCGAA
CAAGGCAGGAAACCGGAGGACGCGAAGATCGACGAAATGGAGGCCCTGTGGCAGGAAGCGAAGCGCAAGTAG

Upstream 100 bases:

>100_bases
CGGCCGTAATACGGCGCGTGCTCGGATTGATCGGGCTTGGAAATCAAAAGGTCGGGCATATTGGTATGTTACATTGAGGG
AAATCGCTCCCCCAAACAGA

Downstream 100 bases:

>100_bases
CGCGGATCGAAATTCGCCAGCTTTTTCAGCTGCCGGAGTTTGAAGAAGTTCTTCAGATGCAGAAGACCATCTGGAGCTTC
GACGATATCGAACTGCTCCC

Product: MazG family protein

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSTENAGEKFQKLVAIMARLRAPDGCPWDREQTFDTIKPYTLEETYEVLDAIDRRQWDELAEELGDFMLQAVFYAQMAAE
QKLFRIDDALDAINEKLVRRHPHVFGEESAASADDVMAIWGRVKGSEQKKKDKPAGLLGGVPRTLPALVEAQQIASRAAG
VGFDWENPEQVLEKLHEELAEFAVARTNAQHDEIEDELGDMLFVMVNLARFVKVDPEQALRRTNAKFRKRWSHIEARLAE
QGRKPEDAKIDEMEALWQEAKRK

Sequences:

>Translated_263_residues
MSTENAGEKFQKLVAIMARLRAPDGCPWDREQTFDTIKPYTLEETYEVLDAIDRRQWDELAEELGDFMLQAVFYAQMAAE
QKLFRIDDALDAINEKLVRRHPHVFGEESAASADDVMAIWGRVKGSEQKKKDKPAGLLGGVPRTLPALVEAQQIASRAAG
VGFDWENPEQVLEKLHEELAEFAVARTNAQHDEIEDELGDMLFVMVNLARFVKVDPEQALRRTNAKFRKRWSHIEARLAE
QGRKPEDAKIDEMEALWQEAKRK
>Mature_262_residues
STENAGEKFQKLVAIMARLRAPDGCPWDREQTFDTIKPYTLEETYEVLDAIDRRQWDELAEELGDFMLQAVFYAQMAAEQ
KLFRIDDALDAINEKLVRRHPHVFGEESAASADDVMAIWGRVKGSEQKKKDKPAGLLGGVPRTLPALVEAQQIASRAAGV
GFDWENPEQVLEKLHEELAEFAVARTNAQHDEIEDELGDMLFVMVNLARFVKVDPEQALRRTNAKFRKRWSHIEARLAEQ
GRKPEDAKIDEMEALWQEAKRK

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=258, Percent_Identity=46.5116279069767, Blast_Score=231, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 30077; Mature: 29946

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTENAGEKFQKLVAIMARLRAPDGCPWDREQTFDTIKPYTLEETYEVLDAIDRRQWDEL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AEELGDFMLQAVFYAQMAAEQKLFRIDDALDAINEKLVRRHPHVFGEESAASADDVMAIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
GRVKGSEQKKKDKPAGLLGGVPRTLPALVEAQQIASRAAGVGFDWENPEQVLEKLHEELA
HHHCCCHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
EFAVARTNAQHDEIEDELGDMLFVMVNLARFVKVDPEQALRRTNAKFRKRWSHIEARLAE
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QGRKPEDAKIDEMEALWQEAKRK
CCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STENAGEKFQKLVAIMARLRAPDGCPWDREQTFDTIKPYTLEETYEVLDAIDRRQWDEL
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AEELGDFMLQAVFYAQMAAEQKLFRIDDALDAINEKLVRRHPHVFGEESAASADDVMAIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
GRVKGSEQKKKDKPAGLLGGVPRTLPALVEAQQIASRAAGVGFDWENPEQVLEKLHEELA
HHHCCCHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
EFAVARTNAQHDEIEDELGDMLFVMVNLARFVKVDPEQALRRTNAKFRKRWSHIEARLAE
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QGRKPEDAKIDEMEALWQEAKRK
CCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]