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The map label for this gene is tgs1 [H]

Identifier: 116620904

GI number: 116620904

Start: 2221142

End: 2222608

Strand: Reverse

Name: tgs1 [H]

Synonym: Acid_1785

Alternate gene names: 116620904

Gene position: 2222608-2221142 (Counterclockwise)

Preceding gene: 116620906

Following gene: 116620903

Centisome position: 22.3

GC content: 65.51

Gene sequence:

>1467_bases
ATGGACAAGAAACCGCCGGTCCTGAGCCGCCGTCTGTCTGGGATCGACGCGGCCTTCCTTTACCTGGAGCGGAAAGAGAT
TCCGCTGAACATCGCCGCCGTCGCGCTTTTCGACGGACCGATCCCGTTCCGTGAGTTCGTCGCCAACATCGAATCCAAGC
TGGACCTGATTCCGCGCTACCGGCAGGTGGCGACGCCGCCGCCGTTTGGCCTGGGCTATCCGACGTGGGAGTACGATCCG
GATTTCGACATCCGGCGGCATATTCTCCGGGTAAAGCTGAAGGCTCCCGGCGGCGAGGCGGAACTGGAAGCGCTGGCCGG
GCGGTTGTTGAGCCAGACGCTGGACCGCGGCAAGCCGCTGTGGGAGATCCACGTAGTGCAGGGCTTGCAGGAGGGGCGCG
GGGCACTGATCGTGCGCATTCACCACGCGCTGGCGGACGGCGTAGCGGGAGCGGCGATGCTGAATATCATGCTGGATCCG
ACGCCGGAAAATTCGCATCCGATTCGCAAGCCGCGGGCGCGGAACCCGGCGGCTCCGCGACCCGAGCCCACGCTCACGGA
GCAGATCAGCGGCGCGATTCAAAGCGCGCTGGAAAACGCGGTGGCGGCGGAGGCGGGCCTGCTCAGCATGGCGGACGGGC
TGCTCAGCGATCAGGGCAAGAGCGCCCTGCAGGAGCTCACCACTCTATTACCCGAGCTATTGCTGCCGCCAGAGCGATTT
CCGTTCAACAAGCCTTGTTCAGGCGACCGTATGTTCTGCTGGGCGGAGGCGGATTTCCAGGAAGTGCGCGAAATTCGTGC
CGCATTGGGCGGCAAAGTCAACGACGTGATCCTGAGCGTGGTGGTGCGTGCGATCGCGCGCTACATCCGGCTGCACGGGG
AGAAAACGGCGGGCCGCTTCCTGCGTGTGGTCTGCCCGGTCAACTTGCGCGGCGGGGAGAACGGCGAATCGCTGGGCAAC
CGGATCACGTTTCTGCCGGTGGTGCTGCCGCTGGACGTGCGCAACCCGGTGAAGATGCTGCACGCGGTGGCGAGCCGGAT
GGAGATCATGAAGAGCGTGCACGCGGCCGAGCTGGTGGCGATCGCGGCCAGTTTCCTGGGGACGGCGCCGGCGCCGATGC
AGGAACTTTTCTGGCGCGGGATCCCGCTGGTTCCGCTGCCGCTGCCGCTGCTCAACATTATCTGCACCAACGTACCGGGC
TCTGCGACGCCGCTGTATACGGTTGGGCGAAGACTGGTGGCGTCGTATCCGCACGTGCCCACCGGGTACGAACTCGGGCT
GGGAGTCGCGGTGCAGAGCTATGACGGGAAGCTCTTTTTCGGGCTGACCGCGGACGCGCACGTGGCGCCGGATGTGGGAC
GGCTGCGCGATTACATCCGGGAGTGCTTTCAGGAGTTGGTGAAGGCGTCGCGCCCGAAGAAAGCGCGGAGGGCTTCGGCG
GAGCAGGCGGAACCGGCGTTAGTGTAG

Upstream 100 bases:

>100_bases
GACCTCAACGAAGCCGATGAGTGGGAAGACGAACATTGCACGGTAGCCGGGCGCGCAAGCTGCCTGTAGGCGCCGCCCGA
GATCTGGAGCCGACCGCCAA

Downstream 100 bases:

>100_bases
AGTATGGGCGAATCCAAATCGGTGCTGCATCCCGGCCTGCACGGGCGCGTCGCGGTGGTCACGGGAGCCAGCCGCGGGAT
CGGCAAGGCACTGGCGATCC

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: TGS1; Probable triacylglycerol synthase tgs1 [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MDKKPPVLSRRLSGIDAAFLYLERKEIPLNIAAVALFDGPIPFREFVANIESKLDLIPRYRQVATPPPFGLGYPTWEYDP
DFDIRRHILRVKLKAPGGEAELEALAGRLLSQTLDRGKPLWEIHVVQGLQEGRGALIVRIHHALADGVAGAAMLNIMLDP
TPENSHPIRKPRARNPAAPRPEPTLTEQISGAIQSALENAVAAEAGLLSMADGLLSDQGKSALQELTTLLPELLLPPERF
PFNKPCSGDRMFCWAEADFQEVREIRAALGGKVNDVILSVVVRAIARYIRLHGEKTAGRFLRVVCPVNLRGGENGESLGN
RITFLPVVLPLDVRNPVKMLHAVASRMEIMKSVHAAELVAIAASFLGTAPAPMQELFWRGIPLVPLPLPLLNIICTNVPG
SATPLYTVGRRLVASYPHVPTGYELGLGVAVQSYDGKLFFGLTADAHVAPDVGRLRDYIRECFQELVKASRPKKARRASA
EQAEPALV

Sequences:

>Translated_488_residues
MDKKPPVLSRRLSGIDAAFLYLERKEIPLNIAAVALFDGPIPFREFVANIESKLDLIPRYRQVATPPPFGLGYPTWEYDP
DFDIRRHILRVKLKAPGGEAELEALAGRLLSQTLDRGKPLWEIHVVQGLQEGRGALIVRIHHALADGVAGAAMLNIMLDP
TPENSHPIRKPRARNPAAPRPEPTLTEQISGAIQSALENAVAAEAGLLSMADGLLSDQGKSALQELTTLLPELLLPPERF
PFNKPCSGDRMFCWAEADFQEVREIRAALGGKVNDVILSVVVRAIARYIRLHGEKTAGRFLRVVCPVNLRGGENGESLGN
RITFLPVVLPLDVRNPVKMLHAVASRMEIMKSVHAAELVAIAASFLGTAPAPMQELFWRGIPLVPLPLPLLNIICTNVPG
SATPLYTVGRRLVASYPHVPTGYELGLGVAVQSYDGKLFFGLTADAHVAPDVGRLRDYIRECFQELVKASRPKKARRASA
EQAEPALV
>Mature_488_residues
MDKKPPVLSRRLSGIDAAFLYLERKEIPLNIAAVALFDGPIPFREFVANIESKLDLIPRYRQVATPPPFGLGYPTWEYDP
DFDIRRHILRVKLKAPGGEAELEALAGRLLSQTLDRGKPLWEIHVVQGLQEGRGALIVRIHHALADGVAGAAMLNIMLDP
TPENSHPIRKPRARNPAAPRPEPTLTEQISGAIQSALENAVAAEAGLLSMADGLLSDQGKSALQELTTLLPELLLPPERF
PFNKPCSGDRMFCWAEADFQEVREIRAALGGKVNDVILSVVVRAIARYIRLHGEKTAGRFLRVVCPVNLRGGENGESLGN
RITFLPVVLPLDVRNPVKMLHAVASRMEIMKSVHAAELVAIAASFLGTAPAPMQELFWRGIPLVPLPLPLLNIICTNVPG
SATPLYTVGRRLVASYPHVPTGYELGLGVAVQSYDGKLFFGLTADAHVAPDVGRLRDYIRECFQELVKASRPKKARRASA
EQAEPALV

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Prefers C(26:0)-CoA over C(18:1)-CoA. TG synthesis activity increases in M.tuberculosis upon oxygen depletion and NO

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 53343; Mature: 53343

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKPPVLSRRLSGIDAAFLYLERKEIPLNIAAVALFDGPIPFREFVANIESKLDLIPRY
CCCCCCHHHHHHCCCCHHHHEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
RQVATPPPFGLGYPTWEYDPDFDIRRHILRVKLKAPGGEAELEALAGRLLSQTLDRGKPL
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCE
WEIHVVQGLQEGRGALIVRIHHALADGVAGAAMLNIMLDPTPENSHPIRKPRARNPAAPR
EEEHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCC
PEPTLTEQISGAIQSALENAVAAEAGLLSMADGLLSDQGKSALQELTTLLPELLLPPERF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PFNKPCSGDRMFCWAEADFQEVREIRAALGGKVNDVILSVVVRAIARYIRLHGEKTAGRF
CCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCE
LRVVCPVNLRGGENGESLGNRITFLPVVLPLDVRNPVKMLHAVASRMEIMKSVHAAELVA
EEEEECEECCCCCCHHHHCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAASFLGTAPAPMQELFWRGIPLVPLPLPLLNIICTNVPGSATPLYTVGRRLVASYPHVP
HHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
TGYELGLGVAVQSYDGKLFFGLTADAHVAPDVGRLRDYIRECFQELVKASRPKKARRASA
CCCCCCCEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCH
EQAEPALV
HCCCCCCC
>Mature Secondary Structure
MDKKPPVLSRRLSGIDAAFLYLERKEIPLNIAAVALFDGPIPFREFVANIESKLDLIPRY
CCCCCCHHHHHHCCCCHHHHEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
RQVATPPPFGLGYPTWEYDPDFDIRRHILRVKLKAPGGEAELEALAGRLLSQTLDRGKPL
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCE
WEIHVVQGLQEGRGALIVRIHHALADGVAGAAMLNIMLDPTPENSHPIRKPRARNPAAPR
EEEHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCC
PEPTLTEQISGAIQSALENAVAAEAGLLSMADGLLSDQGKSALQELTTLLPELLLPPERF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PFNKPCSGDRMFCWAEADFQEVREIRAALGGKVNDVILSVVVRAIARYIRLHGEKTAGRF
CCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCE
LRVVCPVNLRGGENGESLGNRITFLPVVLPLDVRNPVKMLHAVASRMEIMKSVHAAELVA
EEEEECEECCCCCCHHHHCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAASFLGTAPAPMQELFWRGIPLVPLPLPLLNIICTNVPGSATPLYTVGRRLVASYPHVP
HHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
TGYELGLGVAVQSYDGKLFFGLTADAHVAPDVGRLRDYIRECFQELVKASRPKKARRASA
CCCCCCCEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCH
EQAEPALV
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]