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The map label for this gene is garD [H]

Identifier: 116620859

GI number: 116620859

Start: 2161101

End: 2162585

Strand: Direct

Name: garD [H]

Synonym: Acid_1740

Alternate gene names: 116620859

Gene position: 2161101-2162585 (Clockwise)

Preceding gene: 116620858

Following gene: 116620861

Centisome position: 21.69

GC content: 66.4

Gene sequence:

>1485_bases
ATGTACATACGGGTTAATGATCAGGACAACGTCGGGATTATTGTGGAGCCGGAGGGAGTCGTCACGGCGCGGGAGAGGAT
TCCGCAGGCGCACAAGATGGCGCTGTGCGACATCGATGCGGGGACGCCGATTATCAGGTACGGGCAGACTATCGGGATCG
CGAACCGGGCGATCGCCAACGGGAGTTGGGTGCGCGAGGAGTTGATGGACATGCCGGCGGCGCCGCCGTTGGAGGAGCTC
GCGCTCGCGACAGAGATCCCGGTGAAATTGCCGCCGCTCGACGGGTACACGTTCGAGGGCTATCGGAATTCGGACGGCAG
CGTGGGGACGAAGAACATCCTCGGGATCGCGACCACGGTGCAGTGCGTGGCGCCCACGGTGGATTACGCGGTGCGGAGGA
TTCAGGCGGAGTTGCTGCCGCGATTTCCGAATGTGGACGGCGCGGTGGCGGTGACACATTCGTACGGGTGCGGCGTCGCG
ATTGACGCGCCGGGTGCGGCGGTGCCGATTCAGACGCTGAAGCACATCGGGCTGCACGCGAACATGGGCGGGGCGCCGAT
GATCGTCAGCCTGGGTTGCGAGAAGCTGCAGCCGGCGCGACTGTTCGGCAGCGAGCTGCCGATATTGGGCGAGGCCAACG
TGATCCGCCTTCAGGACGAGCGCGGCTTTGCAGCGAGCGTGTCGGCGATTATGGAAGCGGCGGAGCGGCGCCTTGCGGTG
TTGAACCGGCGGCAGCGGCAGACGGTGCCGGCGAGCGAGCTCGTGGTCGGCCTGCAGTGCGGCGGGAGCGATGCGTTCTC
GGGCGTGACGTGCAATCCGGCGGTGGGCTTTGCGGCGGACCTGCTGGTGCGGGCCGGGGCGACCGTGATGTTCTCGGAAG
TCACCGAAGTGCGCGATGCGATCCACCTGCTGACGCCGCGGGCGGAGACGACCGAAGTGGCGCGCGCGCTGATTCGCGAG
ATGCGCTGGTACGACGAATACCTGGCGCGCGGAGGCGCGGACCGAAGCGCCAACACGACGCCGGGGAACAAGCGGGGCGG
ATTGAACAATATCGTGGAGAAGGCGCTGGGCAGCATCGTGAAATCGGGGAGTACGGCCATCCGGGCGGTGGCTTCGCATG
GCGAGAAAGTCACGGCGAAGGGACTGGTTTTCGCGGCCACGCCGGCGAGCGATTTCATTTGCGGCACGCAGCAGCTGGCG
TCGATGAACCTGCACGTGTTCACGACCGGCGAGGGCTCGCCATACGGATTGGCGCTAGTGCCGGTGATCAAGGTCAGTTC
GCGGACGGCGCTGGCGGAGAAGTGGGCGGACCTGATCGATTTGGACGCGGGACGGATCGCGACGGGGCAGGCGACGATCG
CAGAGGTGGGCTGGGAATTGTTCCGGATGATGCTGGAGGTCGCGAGCGGGCGGCGGAAGACGTGGGCGGAGAAGTGGGGG
CTTGGCAACGCGCTCGCGCCGTTCAATCCGGGGCCGGTGACGTAA

Upstream 100 bases:

>100_bases
TTCTCTGGCTGCTCTCCGACGAGGCGTCGTACGTAACGGGCACGTTTATCGATGTGGCGGGTGGGCGTTAGTAAAAAGGG
GTATAAATAGCAGGAGCTAG

Downstream 100 bases:

>100_bases
GAGCCTGTCTTACTGGATCGAGTTGAGGTAGGCGATCAGGTTGACGAGTTCCTGGGGATTGGCGAATTGCGGCCAGGGGA
TGCCGGCCTGTTTCATCCGG

Product: galactarate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MYIRVNDQDNVGIIVEPEGVVTARERIPQAHKMALCDIDAGTPIIRYGQTIGIANRAIANGSWVREELMDMPAAPPLEEL
ALATEIPVKLPPLDGYTFEGYRNSDGSVGTKNILGIATTVQCVAPTVDYAVRRIQAELLPRFPNVDGAVAVTHSYGCGVA
IDAPGAAVPIQTLKHIGLHANMGGAPMIVSLGCEKLQPARLFGSELPILGEANVIRLQDERGFAASVSAIMEAAERRLAV
LNRRQRQTVPASELVVGLQCGGSDAFSGVTCNPAVGFAADLLVRAGATVMFSEVTEVRDAIHLLTPRAETTEVARALIRE
MRWYDEYLARGGADRSANTTPGNKRGGLNNIVEKALGSIVKSGSTAIRAVASHGEKVTAKGLVFAATPASDFICGTQQLA
SMNLHVFTTGEGSPYGLALVPVIKVSSRTALAEKWADLIDLDAGRIATGQATIAEVGWELFRMMLEVASGRRKTWAEKWG
LGNALAPFNPGPVT

Sequences:

>Translated_494_residues
MYIRVNDQDNVGIIVEPEGVVTARERIPQAHKMALCDIDAGTPIIRYGQTIGIANRAIANGSWVREELMDMPAAPPLEEL
ALATEIPVKLPPLDGYTFEGYRNSDGSVGTKNILGIATTVQCVAPTVDYAVRRIQAELLPRFPNVDGAVAVTHSYGCGVA
IDAPGAAVPIQTLKHIGLHANMGGAPMIVSLGCEKLQPARLFGSELPILGEANVIRLQDERGFAASVSAIMEAAERRLAV
LNRRQRQTVPASELVVGLQCGGSDAFSGVTCNPAVGFAADLLVRAGATVMFSEVTEVRDAIHLLTPRAETTEVARALIRE
MRWYDEYLARGGADRSANTTPGNKRGGLNNIVEKALGSIVKSGSTAIRAVASHGEKVTAKGLVFAATPASDFICGTQQLA
SMNLHVFTTGEGSPYGLALVPVIKVSSRTALAEKWADLIDLDAGRIATGQATIAEVGWELFRMMLEVASGRRKTWAEKWG
LGNALAPFNPGPVT
>Mature_494_residues
MYIRVNDQDNVGIIVEPEGVVTARERIPQAHKMALCDIDAGTPIIRYGQTIGIANRAIANGSWVREELMDMPAAPPLEEL
ALATEIPVKLPPLDGYTFEGYRNSDGSVGTKNILGIATTVQCVAPTVDYAVRRIQAELLPRFPNVDGAVAVTHSYGCGVA
IDAPGAAVPIQTLKHIGLHANMGGAPMIVSLGCEKLQPARLFGSELPILGEANVIRLQDERGFAASVSAIMEAAERRLAV
LNRRQRQTVPASELVVGLQCGGSDAFSGVTCNPAVGFAADLLVRAGATVMFSEVTEVRDAIHLLTPRAETTEVARALIRE
MRWYDEYLARGGADRSANTTPGNKRGGLNNIVEKALGSIVKSGSTAIRAVASHGEKVTAKGLVFAATPASDFICGTQQLA
SMNLHVFTTGEGSPYGLALVPVIKVSSRTALAEKWADLIDLDAGRIATGQATIAEVGWELFRMMLEVASGRRKTWAEKWG
LGNALAPFNPGPVT

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=511, Percent_Identity=60.665362035225, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI1789475, Length=499, Percent_Identity=30.8617234468938, Blast_Score=196, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 52440; Mature: 52440

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIRVNDQDNVGIIVEPEGVVTARERIPQAHKMALCDIDAGTPIIRYGQTIGIANRAIAN
CEEEECCCCCEEEEECCCCCCHHHHHCCHHHHEEEEECCCCCCCEECCCEECCCHHHHCC
GSWVREELMDMPAAPPLEELALATEIPVKLPPLDGYTFEGYRNSDGSVGTKNILGIATTV
CHHHHHHHHCCCCCCCHHHHHHHHCCCEECCCCCCEEECCEECCCCCCCCCHHHHHHHHH
QCVAPTVDYAVRRIQAELLPRFPNVDGAVAVTHSYGCGVAIDAPGAAVPIQTLKHIGLHA
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEECCCCCCCHHHHHHHCCCCC
NMGGAPMIVSLGCEKLQPARLFGSELPILGEANVIRLQDERGFAASVSAIMEAAERRLAV
CCCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
LNRRQRQTVPASELVVGLQCGGSDAFSGVTCNPAVGFAADLLVRAGATVMFSEVTEVRDA
HHHHHHCCCCHHHEEEEEEECCCCCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
IHLLTPRAETTEVARALIREMRWYDEYLARGGADRSANTTPGNKRGGLNNIVEKALGSIV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KSGSTAIRAVASHGEKVTAKGLVFAATPASDFICGTQQLASMNLHVFTTGEGSPYGLALV
HCCCHHHHHHHHCCCEEEECCEEEEECCCCHHHCCCHHHHHCEEEEEECCCCCCCCEEEE
PVIKVSSRTALAEKWADLIDLDAGRIATGQATIAEVGWELFRMMLEVASGRRKTWAEKWG
EEEEECCHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
LGNALAPFNPGPVT
CCCCCCCCCCCCCC
>Mature Secondary Structure
MYIRVNDQDNVGIIVEPEGVVTARERIPQAHKMALCDIDAGTPIIRYGQTIGIANRAIAN
CEEEECCCCCEEEEECCCCCCHHHHHCCHHHHEEEEECCCCCCCEECCCEECCCHHHHCC
GSWVREELMDMPAAPPLEELALATEIPVKLPPLDGYTFEGYRNSDGSVGTKNILGIATTV
CHHHHHHHHCCCCCCCHHHHHHHHCCCEECCCCCCEEECCEECCCCCCCCCHHHHHHHHH
QCVAPTVDYAVRRIQAELLPRFPNVDGAVAVTHSYGCGVAIDAPGAAVPIQTLKHIGLHA
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEECCCCCCCHHHHHHHCCCCC
NMGGAPMIVSLGCEKLQPARLFGSELPILGEANVIRLQDERGFAASVSAIMEAAERRLAV
CCCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
LNRRQRQTVPASELVVGLQCGGSDAFSGVTCNPAVGFAADLLVRAGATVMFSEVTEVRDA
HHHHHHCCCCHHHEEEEEEECCCCCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
IHLLTPRAETTEVARALIREMRWYDEYLARGGADRSANTTPGNKRGGLNNIVEKALGSIV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KSGSTAIRAVASHGEKVTAKGLVFAATPASDFICGTQQLASMNLHVFTTGEGSPYGLALV
HCCCHHHHHHHHCCCEEEECCEEEEECCCCHHHCCCHHHHHCEEEEEECCCCCCCCEEEE
PVIKVSSRTALAEKWADLIDLDAGRIATGQATIAEVGWELFRMMLEVASGRRKTWAEKWG
EEEEECCHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
LGNALAPFNPGPVT
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]