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The map label for this gene is pilT [H]

Identifier: 116620707

GI number: 116620707

Start: 1946743

End: 1947894

Strand: Direct

Name: pilT [H]

Synonym: Acid_1587

Alternate gene names: 116620707

Gene position: 1946743-1947894 (Clockwise)

Preceding gene: 116620702

Following gene: 116620709

Centisome position: 19.53

GC content: 64.67

Gene sequence:

>1152_bases
ATGGGCGAGTACGATAACGAACTCTCCAAGATTGTCGCCGAGTTGAACCGCGCGGCGCCAAGCCAGAAGACGGTGCCGCC
GCGCGCGGGCCAGTCCACCGCCTCGCTGGATCAATTGCTCCATGCCGCCGCCGGTCGTGGGGCGTCCGATGTGCTGTTGA
TAGCGGGCGCACCGCCCATGCTGCGGGTCACTGGGAATCTCACCGCCGGCGGCGGCACCGTGCTGGATGCGGAGGACGTG
CGCAGCCTGGTACTCCCGCTGCTGGAGCATGCGCAACTGGAGGAATTGCAGAAGCGCAAGTCCGTGGACTTGAGCTTCGT
GCGCGAGAATATCGGCCGGTTCCGCGTGAATATTCATCACCAGCGCGGGACGCTCGCGGCCAGCATCCGGCTGCTGCCAT
CGCGAATTCCTTCGATCGAGTCTTTGCATTTGCCCGGATCGCTGGCGAAACTGGCCGACCGGCGGCAGGGGCTGGTGCTG
GTGACCGGGCCTACGGGATGCGGCAAGAGTTCCACGCTGGCCGCGCTCATCGACATCGTCAACACGCGGCGCGCTGCCCA
CGTGGTCACGATCGAAGATCCGGTGGAGTACCAGCATGCCAATCGCAGCGCGATCATCGAGCAAATCGAAGTAGGCCGCG
ACACTCCGGATTTTGCGGTGACGCTGCGCAGCATCATGCGGCAGACGCCGGACGTGATCCTGGTGGGCGAGATGCGGGAC
GCCGAGACGATCGCGACCGCGCTGACGGCGGCCGAGACCGGGCACCTGGTTTTCTCGACGCTGCATACCAACGACGCGAT
TCAGGCGACGTCGCGTATTCTCGATTCGTTTCCGGCGGGGAATCAGCCGCAGATTCGTCAGCAGCTTTCGCTCGCACTGG
CCGCAGTGATCGCGCAGCAACTCGTGCCCGGGCTGGATGGCGTCAGCCGCTGGCCGGCGACGGAGATCATGATCGCCACC
GACGCGGTCCGCGCGCTGATTCGGAAGGGCGACGACCACCAATTGCGCTCGCAGATTTCGGTGGGGCGTGCGGAGGGGAT
GATGACGATGGAGCAATCGCTCACGGAGCTGGTCAAGTCCAACAGGATCGCGCGCGATACGGCGTATGCGCACGGGTATC
GCACGGATGATTTGCAGCGGTATTTGGGGTAG

Upstream 100 bases:

>100_bases
CCACGCTGAGCAGTTGTTCCAGCGTCTTCCAATTGTTGTGAATGTCGGAGAATATAAGGAGCTTCACGGTAGAAATGGTA
ACCCAAGGGTAAAGAGGAAA

Downstream 100 bases:

>100_bases
GTGCGGCAGGGCCGCGAGTGGGAAACGGCTTGGATGGGGGGCGCGCGAAGCCGCCTGAAAGGCGGCTGCAGGCAAAATGC
CTGCCCCACAGTGGCAAGCC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MGEYDNELSKIVAELNRAAPSQKTVPPRAGQSTASLDQLLHAAAGRGASDVLLIAGAPPMLRVTGNLTAGGGTVLDAEDV
RSLVLPLLEHAQLEELQKRKSVDLSFVRENIGRFRVNIHHQRGTLAASIRLLPSRIPSIESLHLPGSLAKLADRRQGLVL
VTGPTGCGKSSTLAALIDIVNTRRAAHVVTIEDPVEYQHANRSAIIEQIEVGRDTPDFAVTLRSIMRQTPDVILVGEMRD
AETIATALTAAETGHLVFSTLHTNDAIQATSRILDSFPAGNQPQIRQQLSLALAAVIAQQLVPGLDGVSRWPATEIMIAT
DAVRALIRKGDDHQLRSQISVGRAEGMMTMEQSLTELVKSNRIARDTAYAHGYRTDDLQRYLG

Sequences:

>Translated_383_residues
MGEYDNELSKIVAELNRAAPSQKTVPPRAGQSTASLDQLLHAAAGRGASDVLLIAGAPPMLRVTGNLTAGGGTVLDAEDV
RSLVLPLLEHAQLEELQKRKSVDLSFVRENIGRFRVNIHHQRGTLAASIRLLPSRIPSIESLHLPGSLAKLADRRQGLVL
VTGPTGCGKSSTLAALIDIVNTRRAAHVVTIEDPVEYQHANRSAIIEQIEVGRDTPDFAVTLRSIMRQTPDVILVGEMRD
AETIATALTAAETGHLVFSTLHTNDAIQATSRILDSFPAGNQPQIRQQLSLALAAVIAQQLVPGLDGVSRWPATEIMIAT
DAVRALIRKGDDHQLRSQISVGRAEGMMTMEQSLTELVKSNRIARDTAYAHGYRTDDLQRYLG
>Mature_382_residues
GEYDNELSKIVAELNRAAPSQKTVPPRAGQSTASLDQLLHAAAGRGASDVLLIAGAPPMLRVTGNLTAGGGTVLDAEDVR
SLVLPLLEHAQLEELQKRKSVDLSFVRENIGRFRVNIHHQRGTLAASIRLLPSRIPSIESLHLPGSLAKLADRRQGLVLV
TGPTGCGKSSTLAALIDIVNTRRAAHVVTIEDPVEYQHANRSAIIEQIEVGRDTPDFAVTLRSIMRQTPDVILVGEMRDA
ETIATALTAAETGHLVFSTLHTNDAIQATSRILDSFPAGNQPQIRQQLSLALAAVIAQQLVPGLDGVSRWPATEIMIATD
AVRALIRKGDDHQLRSQISVGRAEGMMTMEQSLTELVKSNRIARDTAYAHGYRTDDLQRYLG

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=330, Percent_Identity=40.3030303030303, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1789723, Length=147, Percent_Identity=48.2993197278912, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786296, Length=162, Percent_Identity=43.8271604938272, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41369; Mature: 41238

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEYDNELSKIVAELNRAAPSQKTVPPRAGQSTASLDQLLHAAAGRGASDVLLIAGAPPM
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCE
LRVTGNLTAGGGTVLDAEDVRSLVLPLLEHAQLEELQKRKSVDLSFVRENIGRFRVNIHH
EEEECCEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEEEEEEE
QRGTLAASIRLLPSRIPSIESLHLPGSLAKLADRRQGLVLVTGPTGCGKSSTLAALIDIV
CCCCHHHHHHHHHHHCCCCCEECCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHH
NTRRAAHVVTIEDPVEYQHANRSAIIEQIEVGRDTPDFAVTLRSIMRQTPDVILVGEMRD
HCCCCEEEEEECCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
AETIATALTAAETGHLVFSTLHTNDAIQATSRILDSFPAGNQPQIRQQLSLALAAVIAQQ
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LVPGLDGVSRWPATEIMIATDAVRALIRKGDDHQLRSQISVGRAEGMMTMEQSLTELVKS
HCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NRIARDTAYAHGYRTDDLQRYLG
CCCHHHHHHHCCCCCHHHHHHCC
>Mature Secondary Structure 
GEYDNELSKIVAELNRAAPSQKTVPPRAGQSTASLDQLLHAAAGRGASDVLLIAGAPPM
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCE
LRVTGNLTAGGGTVLDAEDVRSLVLPLLEHAQLEELQKRKSVDLSFVRENIGRFRVNIHH
EEEECCEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEEEEEEE
QRGTLAASIRLLPSRIPSIESLHLPGSLAKLADRRQGLVLVTGPTGCGKSSTLAALIDIV
CCCCHHHHHHHHHHHCCCCCEECCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHH
NTRRAAHVVTIEDPVEYQHANRSAIIEQIEVGRDTPDFAVTLRSIMRQTPDVILVGEMRD
HCCCCEEEEEECCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
AETIATALTAAETGHLVFSTLHTNDAIQATSRILDSFPAGNQPQIRQQLSLALAAVIAQQ
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LVPGLDGVSRWPATEIMIATDAVRALIRKGDDHQLRSQISVGRAEGMMTMEQSLTELVKS
HCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NRIARDTAYAHGYRTDDLQRYLG
CCCHHHHHHHCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]