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The map label for this gene is yafV [C]

Identifier: 116620675

GI number: 116620675

Start: 1911970

End: 1912809

Strand: Direct

Name: yafV [C]

Synonym: Acid_1555

Alternate gene names: 116620675

Gene position: 1911970-1912809 (Clockwise)

Preceding gene: 116620672

Following gene: 116620676

Centisome position: 19.19

GC content: 64.17

Gene sequence:

>840_bases
ATGGCCTGCTCGCTGGACCCGAATGAGAATCTCGCGAAGGCCGAATGGCGCATTCGCGATGCGGCCGGCAAAGGCGCGCA
GATCGTCTGCGTGCAGGAACTCTTCCGCTCGCAGTATTTCTGCCAGACCGAGAACATCGCCACCTTCGACCTGGCCGAAA
CCATCCCGGGCCCCACCACGGAGAGCTTCACCAGGCTCGCGCGCGAACTCGATGTGGTCATCGTCGGCTCCATTTTCGAA
CGTCGCATGGCGGGAGTCTTCCACAATACGGCGGTGATCATCGATGCCGGCGGCGAACTGCTCGGCCTCTATCGCAAGAT
GCACATCCCCGATGATCCGCGCTTCTACGAGAAGTACTACTTCACGCCCGGCGATCTCGGCTTCCGCTGTTTCGATACCA
AATACGCGCGCATCGCGCCGCTGATCTGCTGGGATCAGTGGTTCCCCGAAGGCGCCCGCATGGCCGCGCTCGGCGGCGCG
CAGATTCTCTTTTACCCCACCGCGATCGGCTTCCACCACTCTGATGCCGCGGAGGCCGGCACTCAGCATAATGCCTGGGA
GACCGTCCAGCGTTCCCACGCCATCGCCAACGGCGTGTACGTCGCCGCCGTCAATCGTGTCGGCCACGAAGGTCCCGAAG
GCGAGGGACTGCAGTTCTGGGGCGGCTCCTTCCTCAGCGATCCCCAGGGCCGCATGCTGGCCAAGGCCGGCGACGCCGAA
GAGACGCTGGTCGTGGAGTGCGACCCGCGCGTGATCGAATCGGTGCGGCGCAACTGGCCCTTCCTCCGCGATCGCCGCAT
CGATGCATACGGGCCCATCGTCAACCGGGTGATCGATTGA

Upstream 100 bases:

>100_bases
AACAGGACGAGCGCACGATGCATGATGCAACTGAAATGATACCAAGGGTCAGTACAATGGAGGAGTGAAACACTCGAAAT
TTCGGGTAGGACTGGTACAA

Downstream 100 bases:

>100_bases
GGCCTCAGGATTTTCGCATGCCCGCGGAGTGGGAGCCGCACCTCGCCACCTGGATCGCCTGGCCCCACAATCGCGCCGAT
TGGCCGGGACGCTTCGCGCC

Product: nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MACSLDPNENLAKAEWRIRDAAGKGAQIVCVQELFRSQYFCQTENIATFDLAETIPGPTTESFTRLARELDVVIVGSIFE
RRMAGVFHNTAVIIDAGGELLGLYRKMHIPDDPRFYEKYYFTPGDLGFRCFDTKYARIAPLICWDQWFPEGARMAALGGA
QILFYPTAIGFHHSDAAEAGTQHNAWETVQRSHAIANGVYVAAVNRVGHEGPEGEGLQFWGGSFLSDPQGRMLAKAGDAE
ETLVVECDPRVIESVRRNWPFLRDRRIDAYGPIVNRVID

Sequences:

>Translated_279_residues
MACSLDPNENLAKAEWRIRDAAGKGAQIVCVQELFRSQYFCQTENIATFDLAETIPGPTTESFTRLARELDVVIVGSIFE
RRMAGVFHNTAVIIDAGGELLGLYRKMHIPDDPRFYEKYYFTPGDLGFRCFDTKYARIAPLICWDQWFPEGARMAALGGA
QILFYPTAIGFHHSDAAEAGTQHNAWETVQRSHAIANGVYVAAVNRVGHEGPEGEGLQFWGGSFLSDPQGRMLAKAGDAE
ETLVVECDPRVIESVRRNWPFLRDRRIDAYGPIVNRVID
>Mature_278_residues
ACSLDPNENLAKAEWRIRDAAGKGAQIVCVQELFRSQYFCQTENIATFDLAETIPGPTTESFTRLARELDVVIVGSIFER
RMAGVFHNTAVIIDAGGELLGLYRKMHIPDDPRFYEKYYFTPGDLGFRCFDTKYARIAPLICWDQWFPEGARMAALGGAQ
ILFYPTAIGFHHSDAAEAGTQHNAWETVQRSHAIANGVYVAAVNRVGHEGPEGEGLQFWGGSFLSDPQGRMLAKAGDAEE
TLVVECDPRVIESVRRNWPFLRDRRIDAYGPIVNRVID

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=269, Percent_Identity=33.457249070632, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI7706509, Length=269, Percent_Identity=32.7137546468402, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI297632350, Length=276, Percent_Identity=28.2608695652174, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI297632348, Length=276, Percent_Identity=28.2608695652174, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI5031947, Length=276, Percent_Identity=28.2608695652174, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI297632346, Length=175, Percent_Identity=29.1428571428571, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17533173, Length=277, Percent_Identity=32.8519855595668, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17556280, Length=283, Percent_Identity=28.2685512367491, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6323383, Length=280, Percent_Identity=28.9285714285714, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322335, Length=305, Percent_Identity=25.2459016393443, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21358471, Length=276, Percent_Identity=32.9710144927536, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17933642, Length=284, Percent_Identity=27.112676056338, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21355835, Length=229, Percent_Identity=29.6943231441048, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 31192; Mature: 31060

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACSLDPNENLAKAEWRIRDAAGKGAQIVCVQELFRSQYFCQTENIATFDLAETIPGPTT
CCCCCCCCCCCHHHHEEEECCCCCCCEEEEHHHHHHHHCEECCCCEEEEEHHHHCCCCCH
ESFTRLARELDVVIVGSIFERRMAGVFHNTAVIIDAGGELLGLYRKMHIPDDPRFYEKYY
HHHHHHHHHCCEEEEHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCCHHHEEE
FTPGDLGFRCFDTKYARIAPLICWDQWFPEGARMAALGGAQILFYPTAIGFHHSDAAEAG
ECCCCCCEEEECCCHHHHHHEEEHHHHCCCCCEEEECCCCEEEEEEEEECCCCCCHHHCC
TQHNAWETVQRSHAIANGVYVAAVNRVGHEGPEGEGLQFWGGSFLSDPQGRMLAKAGDAE
CCCCHHHHHHHHHHHHCCEEEEEEHHHCCCCCCCCCEEEECCCCCCCCCCCEEEECCCCC
ETLVVECDPRVIESVRRNWPFLRDRRIDAYGPIVNRVID
CEEEEECCHHHHHHHHHCCCCHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
ACSLDPNENLAKAEWRIRDAAGKGAQIVCVQELFRSQYFCQTENIATFDLAETIPGPTT
CCCCCCCCCCHHHHEEEECCCCCCCEEEEHHHHHHHHCEECCCCEEEEEHHHHCCCCCH
ESFTRLARELDVVIVGSIFERRMAGVFHNTAVIIDAGGELLGLYRKMHIPDDPRFYEKYY
HHHHHHHHHCCEEEEHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCCHHHEEE
FTPGDLGFRCFDTKYARIAPLICWDQWFPEGARMAALGGAQILFYPTAIGFHHSDAAEAG
ECCCCCCEEEECCCHHHHHHEEEHHHHCCCCCEEEECCCCEEEEEEEEECCCCCCHHHCC
TQHNAWETVQRSHAIANGVYVAAVNRVGHEGPEGEGLQFWGGSFLSDPQGRMLAKAGDAE
CCCCHHHHHHHHHHHHCCEEEEEEHHHCCCCCCCCCEEEECCCCCCCCCCCEEEECCCCC
ETLVVECDPRVIESVRRNWPFLRDRRIDAYGPIVNRVID
CEEEEECCHHHHHHHHHCCCCHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8526497 [H]