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The map label for this gene is lipA [H]

Identifier: 116620622

GI number: 116620622

Start: 1839022

End: 1839948

Strand: Direct

Name: lipA [H]

Synonym: Acid_1501

Alternate gene names: 116620622

Gene position: 1839022-1839948 (Clockwise)

Preceding gene: 116620621

Following gene: 116620623

Centisome position: 18.45

GC content: 64.62

Gene sequence:

>927_bases
GTGACCGATGGGCTGGTGCAGATCGGCGGCAGTGCGCCGCGACCCGCGCGGGCCCGCCTGCCGGAATGGGCGCGCAAGAG
CAAGACGCATTTCCAATCGCTCAACCATTTGAAGCGAGGCCTGCGCGAACTGAAACTCCACACCGTGTGCGAGGAGGCGC
GCTGTCCCAACATCCACGAATGCTTCCACCGCGGAGCGGCAACGTTCATGATATTGGGCGACCTCTGCACTCGCGGCTGC
GGCTTCTGCAGCGTTCGCAAATTGAATCCGCGGAAACACGATGTGCGGCTGGATGAGAACGAGCCGGCCAACGTGGCGCG
AATGGCGCGGGAGATGAAGCTGCGCTACGTGGTGATCACCAGCGTAAATCGCGACGATCTGCCGGACGGCGGGTCGCGGC
ATTTCGCGGAGACTGTCCGCGAGGTGCGGAAGGCGCTGCCCGAGGCGCGCGTGGAAGTGTTGACGCCGGATTTCTGCGGC
GACCTGGACGCGGTCGCGCGCGTGCTGGACGCCGGGCCTCACGTGTTCAACCACAATATGGAAACGGTGCCGCGGCTCTA
CGCGCGAGTTCGTCCGCAGGCGGAATATCGACAGTCGCTGGACGTGCTGGCGTTCGCCCGGAGATTCGCGGAAGGCGTGC
TGGTGAAATCCGGATTCATGGTCGGGTTGGGGGAGCGCGCGGATGAGGTGGAGGCGCTGCTGCGCGACCTGCACGAGGCG
GGCACACAAATCGCGACCATAGGACAGTACCTGCAGCCGACGCGGCGCAACTTGCCGGTGGAGGAATTTGTGGAGCCGCA
TCAATTCGAAGCGTGGCGCGACTACGGCATGGGGCTGGGATTCCAGGCGGTATTCAGCGGTCCGTTGGTGCGTAGTTCGT
ATATGGCCGATCTGGTCAGCGAGGAGGCCCAGCGGGGTCTCGGATGA

Upstream 100 bases:

>100_bases
TCACGCGCGTGCTGGTGGGTCCTTTCGGGGACACGAATGCGATGGGCCGCGCGAAGACGGAACTGGAGAATGCGGGCTTC
CGGCCCGTGCGGTACAAGCC

Downstream 100 bases:

>100_bases
ACTGGCTCTTGGCGCTGGCGTCCGCCGGGATGCTGGTGCTGGCGTTTCCGCAATTCTCCCTGGTGTGGCTGGCTCCGGTG
GCATTGGCGCCGCTGCTGGT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTDGLVQIGGSAPRPARARLPEWARKSKTHFQSLNHLKRGLRELKLHTVCEEARCPNIHECFHRGAATFMILGDLCTRGC
GFCSVRKLNPRKHDVRLDENEPANVARMAREMKLRYVVITSVNRDDLPDGGSRHFAETVREVRKALPEARVEVLTPDFCG
DLDAVARVLDAGPHVFNHNMETVPRLYARVRPQAEYRQSLDVLAFARRFAEGVLVKSGFMVGLGERADEVEALLRDLHEA
GTQIATIGQYLQPTRRNLPVEEFVEPHQFEAWRDYGMGLGFQAVFSGPLVRSSYMADLVSEEAQRGLG

Sequences:

>Translated_308_residues
MTDGLVQIGGSAPRPARARLPEWARKSKTHFQSLNHLKRGLRELKLHTVCEEARCPNIHECFHRGAATFMILGDLCTRGC
GFCSVRKLNPRKHDVRLDENEPANVARMAREMKLRYVVITSVNRDDLPDGGSRHFAETVREVRKALPEARVEVLTPDFCG
DLDAVARVLDAGPHVFNHNMETVPRLYARVRPQAEYRQSLDVLAFARRFAEGVLVKSGFMVGLGERADEVEALLRDLHEA
GTQIATIGQYLQPTRRNLPVEEFVEPHQFEAWRDYGMGLGFQAVFSGPLVRSSYMADLVSEEAQRGLG
>Mature_307_residues
TDGLVQIGGSAPRPARARLPEWARKSKTHFQSLNHLKRGLRELKLHTVCEEARCPNIHECFHRGAATFMILGDLCTRGCG
FCSVRKLNPRKHDVRLDENEPANVARMAREMKLRYVVITSVNRDDLPDGGSRHFAETVREVRKALPEARVEVLTPDFCGD
LDAVARVLDAGPHVFNHNMETVPRLYARVRPQAEYRQSLDVLAFARRFAEGVLVKSGFMVGLGERADEVEALLRDLHEAG
TQIATIGQYLQPTRRNLPVEEFVEPHQFEAWRDYGMGLGFQAVFSGPLVRSSYMADLVSEEAQRGLG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=287, Percent_Identity=49.4773519163763, Blast_Score=272, Evalue=2e-73,
Organism=Homo sapiens, GI37577164, Length=251, Percent_Identity=49.003984063745, Blast_Score=231, Evalue=4e-61,
Organism=Escherichia coli, GI1786846, Length=290, Percent_Identity=47.2413793103448, Blast_Score=263, Evalue=7e-72,
Organism=Caenorhabditis elegans, GI32564533, Length=289, Percent_Identity=45.3287197231834, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324770, Length=284, Percent_Identity=43.3098591549296, Blast_Score=239, Evalue=3e-64,
Organism=Drosophila melanogaster, GI221513272, Length=289, Percent_Identity=49.4809688581315, Blast_Score=269, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34658; Mature: 34527

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGLVQIGGSAPRPARARLPEWARKSKTHFQSLNHLKRGLRELKLHTVCEEARCPNIHE
CCCCCEECCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
CFHRGAATFMILGDLCTRGCGFCSVRKLNPRKHDVRLDENEPANVARMAREMKLRYVVIT
HHHCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEE
SVNRDDLPDGGSRHFAETVREVRKALPEARVEVLTPDFCGDLDAVARVLDAGPHVFNHNM
ECCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCCCHHCCCHHHHHHHHHCCCHHHCCCH
ETVPRLYARVRPQAEYRQSLDVLAFARRFAEGVLVKSGFMVGLGERADEVEALLRDLHEA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHH
GTQIATIGQYLQPTRRNLPVEEFVEPHQFEAWRDYGMGLGFQAVFSGPLVRSSYMADLVS
CHHHHHHHHHHCHHHCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHH
EEAQRGLG
HHHHHCCC
>Mature Secondary Structure 
TDGLVQIGGSAPRPARARLPEWARKSKTHFQSLNHLKRGLRELKLHTVCEEARCPNIHE
CCCCEECCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
CFHRGAATFMILGDLCTRGCGFCSVRKLNPRKHDVRLDENEPANVARMAREMKLRYVVIT
HHHCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEE
SVNRDDLPDGGSRHFAETVREVRKALPEARVEVLTPDFCGDLDAVARVLDAGPHVFNHNM
ECCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCCCHHCCCHHHHHHHHHCCCHHHCCCH
ETVPRLYARVRPQAEYRQSLDVLAFARRFAEGVLVKSGFMVGLGERADEVEALLRDLHEA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHH
GTQIATIGQYLQPTRRNLPVEEFVEPHQFEAWRDYGMGLGFQAVFSGPLVRSSYMADLVS
CHHHHHHHHHHCHHHCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHH
EEAQRGLG
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA