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The map label for this gene is pehX [H]

Identifier: 116620373

GI number: 116620373

Start: 1547608

End: 1548948

Strand: Direct

Name: pehX [H]

Synonym: Acid_1250

Alternate gene names: 116620373

Gene position: 1547608-1548948 (Clockwise)

Preceding gene: 116620371

Following gene: 116620374

Centisome position: 15.53

GC content: 63.46

Gene sequence:

>1341_bases
ATGAAATCGACTTGCCGGGTTGCGCTGGTCGGCCTCCTGGCCGCATGCGCGTGGAGCGCGGAATTCGACGTGAAAACGTT
CGGTGCGGCCGGAGACGGGAAGAAGAAAGACACGGCTGCGATCGCGCGCGCCATCGACGCGGCCGCCAAGGCGGGCGGCG
GCACGGTGGTGGTATCGCCGGGACGCTACCTGACCGGCGCGCTGACGCTGAAGAGCAATGTGACGCTGGACGTGGAGGCG
GGCGCGACCCTGCTGGGCAGTCCGGACCCCGAGGACTATCCGCTGCGCGAGAACGTGTGGGGCGAAAAGAAGGAATACAG
CTCGCTGATCTACGCGGACGGCGCGGTGCACATCACGATTCGCGGGCGTGGCACGATCGACGGGCAGGGGCAGGCCTGGT
GGAAACGCATGGGCTGGCCGGACCGCCGCAAAATCGCGCCGGAGCAGCGTACGGCGGCGGAGCGCGCGGAACTGGCGAAG
CTGGAATACGGCCGTCCGCACATGATCAAGCTGGTGCGGTCGAAACACGTGGTAATCGAGGGGCTGCATCTGATCAACTC
GGCGTCGTGGACGGTGAATCCGCTGTTGTGCGAGTTCGTGCGGATCGACGGGATCACGATCGAAAACCCGGTGCCTTCGC
CGAATACCGATGGAATTAATCCGGAGAGCTGCCGCAATGTGCAGATTCTGAATTCGCGGATCGATGTGGGCGACGATTGC
GTCACCTTGAAATCGGGGAAGGACGAGGCCGGCCGGCGCGTGGGGCGGCCGGACGAGAACATCACGATCACGAATTGCGT
CATGCTCAAGGGCCACGGCGCGGTCACGATCGGCAGCGAGATGTCGGGCGGCGTGCGCAACGTGGTGGTGAGCAACTGCG
TGTTCCAGGGGACCGACGTGGGCATCCGGGTGAAATCGCAGCGCGGGCGCGGCGGCATCGTCGAGGGCTTCGTGGTCTCG
AACGTGGTCATGCAGGATGTGGCGAGCGCCTTTACGCTCACCAGCTTTTACGCTGGCACGGACAAGCCGGGCGATCTGTT
TCCGGTTGGCGAGGGGACGCCGCGGCTGCGCGATTTCCGTTTCAGCAACATCACCGCGCGCGGATCGAAGACGGCAGGGC
AAATCACTGGGTTGAAAGAGATGCCGATTGAGAACATCACATTTACAGGCGTGCGGATTCAAGCGGAGACGGGCATGAAG
ATCACGAACGCTAAAGACGTCACATTTCAGGACGTGATCATCGAGGCTGCAAAGGGAGACGCGGTTTCGGTGGTCGATAG
CGTGGGGATCGAACTGGGAAGATTGAAGGGCCGAGCCGCGACCGTCAGGGAGCGTCCTTAG

Upstream 100 bases:

>100_bases
GGACCATCGGGGGCGACAACGAATTCGCGATCGGCGGCGCGCACCGTAATGGCAAATAATAAGACCGCAATGACCTTCAT
CTGCCCGTTATTATAGACGC

Downstream 100 bases:

>100_bases
GTAATCCGGTACCAACGGTTCAAGAGCGCCAAAATCTTGCCGATATGGGAACCGGATGCATGCATTGATCGGGATTGCGG
TAGTGTTCGCCGCTGTGATT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Exo-PG [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKSTCRVALVGLLAACAWSAEFDVKTFGAAGDGKKKDTAAIARAIDAAAKAGGGTVVVSPGRYLTGALTLKSNVTLDVEA
GATLLGSPDPEDYPLRENVWGEKKEYSSLIYADGAVHITIRGRGTIDGQGQAWWKRMGWPDRRKIAPEQRTAAERAELAK
LEYGRPHMIKLVRSKHVVIEGLHLINSASWTVNPLLCEFVRIDGITIENPVPSPNTDGINPESCRNVQILNSRIDVGDDC
VTLKSGKDEAGRRVGRPDENITITNCVMLKGHGAVTIGSEMSGGVRNVVVSNCVFQGTDVGIRVKSQRGRGGIVEGFVVS
NVVMQDVASAFTLTSFYAGTDKPGDLFPVGEGTPRLRDFRFSNITARGSKTAGQITGLKEMPIENITFTGVRIQAETGMK
ITNAKDVTFQDVIIEAAKGDAVSVVDSVGIELGRLKGRAATVRERP

Sequences:

>Translated_446_residues
MKSTCRVALVGLLAACAWSAEFDVKTFGAAGDGKKKDTAAIARAIDAAAKAGGGTVVVSPGRYLTGALTLKSNVTLDVEA
GATLLGSPDPEDYPLRENVWGEKKEYSSLIYADGAVHITIRGRGTIDGQGQAWWKRMGWPDRRKIAPEQRTAAERAELAK
LEYGRPHMIKLVRSKHVVIEGLHLINSASWTVNPLLCEFVRIDGITIENPVPSPNTDGINPESCRNVQILNSRIDVGDDC
VTLKSGKDEAGRRVGRPDENITITNCVMLKGHGAVTIGSEMSGGVRNVVVSNCVFQGTDVGIRVKSQRGRGGIVEGFVVS
NVVMQDVASAFTLTSFYAGTDKPGDLFPVGEGTPRLRDFRFSNITARGSKTAGQITGLKEMPIENITFTGVRIQAETGMK
ITNAKDVTFQDVIIEAAKGDAVSVVDSVGIELGRLKGRAATVRERP
>Mature_446_residues
MKSTCRVALVGLLAACAWSAEFDVKTFGAAGDGKKKDTAAIARAIDAAAKAGGGTVVVSPGRYLTGALTLKSNVTLDVEA
GATLLGSPDPEDYPLRENVWGEKKEYSSLIYADGAVHITIRGRGTIDGQGQAWWKRMGWPDRRKIAPEQRTAAERAELAK
LEYGRPHMIKLVRSKHVVIEGLHLINSASWTVNPLLCEFVRIDGITIENPVPSPNTDGINPESCRNVQILNSRIDVGDDC
VTLKSGKDEAGRRVGRPDENITITNCVMLKGHGAVTIGSEMSGGVRNVVVSNCVFQGTDVGIRVKSQRGRGGIVEGFVVS
NVVMQDVASAFTLTSFYAGTDKPGDLFPVGEGTPRLRDFRFSNITARGSKTAGQITGLKEMPIENITFTGVRIQAETGMK
ITNAKDVTFQDVIIEAAKGDAVSVVDSVGIELGRLKGRAATVRERP

Specific function: Contributes significantly to bacterial utilization of polygalacturonate and the induction of pectate lyase in the presence of extracellular pectic polymers [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR000743
- InterPro:   IPR013783
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00041 fn3; PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.82 [H]

Molecular weight: Translated: 47721; Mature: 47721

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00502 POLYGALACTURONASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTCRVALVGLLAACAWSAEFDVKTFGAAGDGKKKDTAAIARAIDAAAKAGGGTVVVSP
CCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECC
GRYLTGALTLKSNVTLDVEAGATLLGSPDPEDYPLRENVWGEKKEYSSLIYADGAVHITI
CCEEEEEEEEECCEEEEECCCEEEECCCCCCCCCCCCCCCCCCHHHCEEEEECCEEEEEE
RGRGTIDGQGQAWWKRMGWPDRRKIAPEQRTAAERAELAKLEYGRPHMIKLVRSKHVVIE
ECCCCCCCCCHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCEEEEE
GLHLINSASWTVNPLLCEFVRIDGITIENPVPSPNTDGINPESCRNVQILNSRIDVGDDC
EEHHHCCCCCEECHHHHHHHEECCEEECCCCCCCCCCCCCHHHCCCEEEEECCCCCCCCE
VTLKSGKDEAGRRVGRPDENITITNCVMLKGHGAVTIGSEMSGGVRNVVVSNCVFQGTDV
EEEECCCCHHHHCCCCCCCCEEEEEEEEEECCCEEEECCCCCCCHHHHHHHHEEEECCCC
GIRVKSQRGRGGIVEGFVVSNVVMQDVASAFTLTSFYAGTDKPGDLFPVGEGTPRLRDFR
EEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCCCCCCCEEEE
FSNITARGSKTAGQITGLKEMPIENITFTGVRIQAETGMKITNAKDVTFQDVIIEAAKGD
ECCEEECCCCCCHHCCCHHHCCCCCEEEEEEEEEECCCEEEECCCCCCHHHHEEECCCCC
AVSVVDSVGIELGRLKGRAATVRERP
EEEHHHHCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKSTCRVALVGLLAACAWSAEFDVKTFGAAGDGKKKDTAAIARAIDAAAKAGGGTVVVSP
CCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECC
GRYLTGALTLKSNVTLDVEAGATLLGSPDPEDYPLRENVWGEKKEYSSLIYADGAVHITI
CCEEEEEEEEECCEEEEECCCEEEECCCCCCCCCCCCCCCCCCHHHCEEEEECCEEEEEE
RGRGTIDGQGQAWWKRMGWPDRRKIAPEQRTAAERAELAKLEYGRPHMIKLVRSKHVVIE
ECCCCCCCCCHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCEEEEE
GLHLINSASWTVNPLLCEFVRIDGITIENPVPSPNTDGINPESCRNVQILNSRIDVGDDC
EEHHHCCCCCEECHHHHHHHEECCEEECCCCCCCCCCCCCHHHCCCEEEEECCCCCCCCE
VTLKSGKDEAGRRVGRPDENITITNCVMLKGHGAVTIGSEMSGGVRNVVVSNCVFQGTDV
EEEECCCCHHHHCCCCCCCCEEEEEEEEEECCCEEEECCCCCCCHHHHHHHHEEEECCCC
GIRVKSQRGRGGIVEGFVVSNVVMQDVASAFTLTSFYAGTDKPGDLFPVGEGTPRLRDFR
EEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCCCCCCCEEEE
FSNITARGSKTAGQITGLKEMPIENITFTGVRIQAETGMKITNAKDVTFQDVIIEAAKGD
ECCEEECCCCCCHHCCCHHHCCCCCEEEEEEEEEECCCEEEECCCCCCHHHHEEECCCCC
AVSVVDSVGIELGRLKGRAATVRERP
EEEHHHHCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2168372; 1409594 [H]