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The map label for this gene is araD [H]

Identifier: 116620353

GI number: 116620353

Start: 1520251

End: 1520940

Strand: Reverse

Name: araD [H]

Synonym: Acid_1230

Alternate gene names: 116620353

Gene position: 1520940-1520251 (Counterclockwise)

Preceding gene: 116620354

Following gene: 116620352

Centisome position: 15.26

GC content: 66.52

Gene sequence:

>690_bases
GTGCTGGAAACGCTGCGAGCTGAAGTCCTGGAAGCCAATCTCGAACTCGTCCGCCGCGGCCTGGTGGTCTACACCTTCGG
CAACGCCAGCGCCGTATCGCGCGAGCACGGCCTCTGCGTGATCAAACCGAGCGGCGTCCCGTACGAGAAGATGACCCCCG
CCGACATGGTGATCACGGACCTTCACGGCAAGATCGTCGAAGGAGTCCTGCGCCCCTCATCGGATCTGGCGACCCATGTC
GCGCTCTATCGCGCCTTCCCCACCATCGGCGGCGTGGTTCACACGCACTCCCGCCACGCCACCGCATGGGCTCAGGCCCA
GCGCGAGATCCCCTGCTTCGGCACCACCCACGCGGACTACTTCCACGGCCCCATCCCGCTGACCCCGACCCTGACCCGTG
AAGAGATCGAAGGCGAGTACGAGGCCAACACCGGCACCGCCATCATTCGCCGCATGCAAGGCCTGGACCCGCTGGCCTGC
CCGGCCTGCCTGGTGGCCGGCCATGCCCCCTTCTGCTGGGACAAGACCGTGACCGACGCCGCCCACATGGCCGTCATCGT
CGAAGAAATCGCGGCCATGGCCTGGCAAACCGTCACCATCAACCCCCACGCCGCCGCAATCGCCGAGCCTCTCCGCGACA
AACACCACTTCCGCAAACACGGCCCCACCGCCTACTACGGCCAGAAGTAG

Upstream 100 bases:

>100_bases
GAACTCTTTCACCTTTACAATAAATTGTACTTTGCATTCGGCAACGGCCAGGAGCTGCCCCAATTGCGCCGCATCGCCGC
CCACACCCGAGGAGGAAAAA

Downstream 100 bases:

>100_bases
CTTTCCGCCGGCGTCGCGTTAAAATTGGAGTTTGCCCGAAGCCCAGTCCTTCTCCGTCAAACGCGCCCAGGTCGAATTCC
ATAACTTCGCCTCCCTCGGC

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: L-ascorbate utilization protein F; Phosphoribulose isomerase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MLETLRAEVLEANLELVRRGLVVYTFGNASAVSREHGLCVIKPSGVPYEKMTPADMVITDLHGKIVEGVLRPSSDLATHV
ALYRAFPTIGGVVHTHSRHATAWAQAQREIPCFGTTHADYFHGPIPLTPTLTREEIEGEYEANTGTAIIRRMQGLDPLAC
PACLVAGHAPFCWDKTVTDAAHMAVIVEEIAAMAWQTVTINPHAAAIAEPLRDKHHFRKHGPTAYYGQK

Sequences:

>Translated_229_residues
MLETLRAEVLEANLELVRRGLVVYTFGNASAVSREHGLCVIKPSGVPYEKMTPADMVITDLHGKIVEGVLRPSSDLATHV
ALYRAFPTIGGVVHTHSRHATAWAQAQREIPCFGTTHADYFHGPIPLTPTLTREEIEGEYEANTGTAIIRRMQGLDPLAC
PACLVAGHAPFCWDKTVTDAAHMAVIVEEIAAMAWQTVTINPHAAAIAEPLRDKHHFRKHGPTAYYGQK
>Mature_229_residues
MLETLRAEVLEANLELVRRGLVVYTFGNASAVSREHGLCVIKPSGVPYEKMTPADMVITDLHGKIVEGVLRPSSDLATHV
ALYRAFPTIGGVVHTHSRHATAWAQAQREIPCFGTTHADYFHGPIPLTPTLTREEIEGEYEANTGTAIIRRMQGLDPLAC
PACLVAGHAPFCWDKTVTDAAHMAVIVEEIAAMAWQTVTINPHAAAIAEPLRDKHHFRKHGPTAYYGQK

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790642, Length=228, Percent_Identity=57.4561403508772, Blast_Score=276, Evalue=6e-76,
Organism=Escherichia coli, GI1786247, Length=231, Percent_Identity=53.2467532467532, Blast_Score=244, Evalue=4e-66,
Organism=Escherichia coli, GI1790008, Length=231, Percent_Identity=52.8138528138528, Blast_Score=237, Evalue=5e-64,
Organism=Escherichia coli, GI1789164, Length=128, Percent_Identity=38.28125, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: =5.1.3.4 [H]

Molecular weight: Translated: 25062; Mature: 25062

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLETLRAEVLEANLELVRRGLVVYTFGNASAVSREHGLCVIKPSGVPYEKMTPADMVITD
CCHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHCCEEEECCCCCCCCCCCCHHHEEHH
LHGKIVEGVLRPSSDLATHVALYRAFPTIGGVVHTHSRHATAWAQAQREIPCFGTTHADY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHCCCCCCCCCCCCC
FHGPIPLTPTLTREEIEGEYEANTGTAIIRRMQGLDPLACPACLVAGHAPFCWDKTVTDA
CCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHH
AHMAVIVEEIAAMAWQTVTINPHAAAIAEPLRDKHHFRKHGPTAYYGQK
HHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLETLRAEVLEANLELVRRGLVVYTFGNASAVSREHGLCVIKPSGVPYEKMTPADMVITD
CCHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHCCEEEECCCCCCCCCCCCHHHEEHH
LHGKIVEGVLRPSSDLATHVALYRAFPTIGGVVHTHSRHATAWAQAQREIPCFGTTHADY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHCCCCCCCCCCCCC
FHGPIPLTPTLTREEIEGEYEANTGTAIIRRMQGLDPLACPACLVAGHAPFCWDKTVTDA
CCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHH
AHMAVIVEEIAAMAWQTVTINPHAAAIAEPLRDKHHFRKHGPTAYYGQK
HHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA