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The map label for this gene is mnmE

Identifier: 116620213

GI number: 116620213

Start: 1341495

End: 1342829

Strand: Reverse

Name: mnmE

Synonym: Acid_1088

Alternate gene names: 116620213

Gene position: 1342829-1341495 (Counterclockwise)

Preceding gene: 116620214

Following gene: 116620208

Centisome position: 13.47

GC content: 64.79

Gene sequence:

>1335_bases
ATGCTGGGAGCTGAGACCATTGTCGCGATTGCAACACCGCCGGGGCGGGCGGGGATTGGGATGGTGCGCGTTTCGGGGCC
GGATGCGCGAGCGATTGTTTCCCGATTCGTAACTTCGATCGCCTGGCAGCCATGGCAGGCGCATCTCGCCAAGTTGCTTG
ACCAGCAGGGGCACGAGATCGACCAGGTGATCGTCACCTTCTTTGAACGCCCGCGGTCATACACCGCGGAAGACCTGGTC
GAGATCACGTGCCATGGATCGCCGGTGGTTCTTCGCCACGCGGTAGAGCGCGCGCTCGAGGCCGGCGCACGTCTGGCACG
GCCCGGCGAATTTACGCTGCGCGCCTTCCTCAACGGCCGCATCGACCTGCCGCGCGCGGAAGCAGTCCGCGACCTGATCG
ATGCCACCACGCTCTACCAGGCACGCATTGCCGCGCAGCAGGTGGAAGGCTCGGTTTCGCGGCGGATCGCGCCGCTCAAG
GAGCAGCTGCTCGAACTGATCGCGCTCCTGGAAGCCGGCATCGATTTTGCCGAGGACGACATTTCGGTGGCTCCGCCGGC
CGAAATTCTGCGCCGGCTGTCGCCGGTGCTGGAAGGCACGGAGCGGCTCGCCGCCAGTTTTCAATATGGCGGTCTCGTGC
ACCAGGGGCTTACGCTGGCAATTGTCGGCCGCCCCAACGTCGGCAAGAGCAGCCTGTTCAACCGGTTGCTGGAACAGGAC
CGCGCCATCGTCACCGAGATCCCCGGCACTACGCGCGACGTGGTCAGCGAAACGGCTGCGATCGGCGGCATTCCGGTGAA
GCTCTACGACACCGCGGGCATTCGCGAGACCGAAGAGCGAGTGGAATCGCTGGGCATCGAGCGTAGCTACCAGGCGATCG
TAGATTCCGACCTCGCGCTGGTGGTGCTCGATCTCACCCGTCCTGTGACTGCGGAAGACGCGAGCCTGATTGCGCGCGCC
GGCGCTCGCGCTCTGATCGTGGCCAACAAGTGCGATCTGCCGGCGGCGGGAGATGTTCCCGCTGAGGCGATCCCGGTTTC
GGCGCTTACCGGGCAAGGCGTGCCGGAGTTGCGCTCCGCGATTCTGGATGCAGTTGCGCCAAAGGGCGTGCTGGAGCACG
AGACCGGCTTCATCACCAGCCTGCGACACGAGCAATTGTTGCGCGAGGCCGTGGAATATCTGCAGAAGGCGCGCGAGGCG
GTGGCATCGATCCCGCACGAGATGCTGCTGCTGGATCTCTACGCCGCGCTCCGGCCGATCGACGCCATCACGGGCGCCAC
TACCGCCGACGATATCCTTAATCGCATTTTCTCGACGTTTTGCATCGGCAAGTGA

Upstream 100 bases:

>100_bases
GATGTTTGCGGCCTGCGAGCAAACAAGGGCTGTGTAATACAAGATCGGTCTTGGTGGCGCATGCTTTAAGCTTGCGCTGT
AATCAGGTATTTGAAACATC

Downstream 100 bases:

>100_bases
GAGTTCTCGCATGGACCTTCACACGTGATGTACGTATAGTGGCCTTGTCCCCGCTAAAGCTGGCGGCGCTCATCATGGAT
CCAGGCGATTGCGTCCGCTT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MLGAETIVAIATPPGRAGIGMVRVSGPDARAIVSRFVTSIAWQPWQAHLAKLLDQQGHEIDQVIVTFFERPRSYTAEDLV
EITCHGSPVVLRHAVERALEAGARLARPGEFTLRAFLNGRIDLPRAEAVRDLIDATTLYQARIAAQQVEGSVSRRIAPLK
EQLLELIALLEAGIDFAEDDISVAPPAEILRRLSPVLEGTERLAASFQYGGLVHQGLTLAIVGRPNVGKSSLFNRLLEQD
RAIVTEIPGTTRDVVSETAAIGGIPVKLYDTAGIRETEERVESLGIERSYQAIVDSDLALVVLDLTRPVTAEDASLIARA
GARALIVANKCDLPAAGDVPAEAIPVSALTGQGVPELRSAILDAVAPKGVLEHETGFITSLRHEQLLREAVEYLQKAREA
VASIPHEMLLLDLYAALRPIDAITGATTADDILNRIFSTFCIGK

Sequences:

>Translated_444_residues
MLGAETIVAIATPPGRAGIGMVRVSGPDARAIVSRFVTSIAWQPWQAHLAKLLDQQGHEIDQVIVTFFERPRSYTAEDLV
EITCHGSPVVLRHAVERALEAGARLARPGEFTLRAFLNGRIDLPRAEAVRDLIDATTLYQARIAAQQVEGSVSRRIAPLK
EQLLELIALLEAGIDFAEDDISVAPPAEILRRLSPVLEGTERLAASFQYGGLVHQGLTLAIVGRPNVGKSSLFNRLLEQD
RAIVTEIPGTTRDVVSETAAIGGIPVKLYDTAGIRETEERVESLGIERSYQAIVDSDLALVVLDLTRPVTAEDASLIARA
GARALIVANKCDLPAAGDVPAEAIPVSALTGQGVPELRSAILDAVAPKGVLEHETGFITSLRHEQLLREAVEYLQKAREA
VASIPHEMLLLDLYAALRPIDAITGATTADDILNRIFSTFCIGK
>Mature_444_residues
MLGAETIVAIATPPGRAGIGMVRVSGPDARAIVSRFVTSIAWQPWQAHLAKLLDQQGHEIDQVIVTFFERPRSYTAEDLV
EITCHGSPVVLRHAVERALEAGARLARPGEFTLRAFLNGRIDLPRAEAVRDLIDATTLYQARIAAQQVEGSVSRRIAPLK
EQLLELIALLEAGIDFAEDDISVAPPAEILRRLSPVLEGTERLAASFQYGGLVHQGLTLAIVGRPNVGKSSLFNRLLEQD
RAIVTEIPGTTRDVVSETAAIGGIPVKLYDTAGIRETEERVESLGIERSYQAIVDSDLALVVLDLTRPVTAEDASLIARA
GARALIVANKCDLPAAGDVPAEAIPVSALTGQGVPELRSAILDAVAPKGVLEHETGFITSLRHEQLLREAVEYLQKAREA
VASIPHEMLLLDLYAALRPIDAITGATTADDILNRIFSTFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=469, Percent_Identity=34.1151385927505, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI193082993, Length=457, Percent_Identity=34.1356673960613, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=32.0717131474104, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI2367268, Length=457, Percent_Identity=39.6061269146608, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI87082120, Length=122, Percent_Identity=40.1639344262295, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17507259, Length=454, Percent_Identity=30.6167400881057, Blast_Score=179, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=489, Percent_Identity=30.4703476482618, Blast_Score=189, Evalue=9e-49,
Organism=Drosophila melanogaster, GI45550813, Length=470, Percent_Identity=30.8510638297872, Blast_Score=182, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_SOLUE (Q02A42)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_822369.1
- ProteinModelPortal:   Q02A42
- STRING:   Q02A42
- GeneID:   4430738
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_1088
- NMPDR:   fig|234267.9.peg.1030
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q02A42
- ProtClustDB:   CLSK775264
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 47798; Mature: 47798

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 81-81 BINDING 120-120 BINDING 444-444

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGAETIVAIATPPGRAGIGMVRVSGPDARAIVSRFVTSIAWQPWQAHLAKLLDQQGHEI
CCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHH
DQVIVTFFERPRSYTAEDLVEITCHGSPVVLRHAVERALEAGARLARPGEFTLRAFLNGR
HHHHHHHHHCCCCCCHHHHEEEEECCCHHHHHHHHHHHHHHCHHCCCCCHHHHHHHHCCC
IDLPRAEAVRDLIDATTLYQARIAAQQVEGSVSRRIAPLKEQLLELIALLEAGIDFAEDD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ISVAPPAEILRRLSPVLEGTERLAASFQYGGLVHQGLTLAIVGRPNVGKSSLFNRLLEQD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEEECCCCCHHHHHHHHHHHC
RAIVTEIPGTTRDVVSETAAIGGIPVKLYDTAGIRETEERVESLGIERSYQAIVDSDLAL
CCEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHCCCCEE
VVLDLTRPVTAEDASLIARAGARALIVANKCDLPAAGDVPAEAIPVSALTGQGVPELRSA
EEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHCCCHHHCCCCCHHHHHH
ILDAVAPKGVLEHETGFITSLRHEQLLREAVEYLQKAREAVASIPHEMLLLDLYAALRPI
HHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
DAITGATTADDILNRIFSTFCIGK
HHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLGAETIVAIATPPGRAGIGMVRVSGPDARAIVSRFVTSIAWQPWQAHLAKLLDQQGHEI
CCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHH
DQVIVTFFERPRSYTAEDLVEITCHGSPVVLRHAVERALEAGARLARPGEFTLRAFLNGR
HHHHHHHHHCCCCCCHHHHEEEEECCCHHHHHHHHHHHHHHCHHCCCCCHHHHHHHHCCC
IDLPRAEAVRDLIDATTLYQARIAAQQVEGSVSRRIAPLKEQLLELIALLEAGIDFAEDD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ISVAPPAEILRRLSPVLEGTERLAASFQYGGLVHQGLTLAIVGRPNVGKSSLFNRLLEQD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEEECCCCCHHHHHHHHHHHC
RAIVTEIPGTTRDVVSETAAIGGIPVKLYDTAGIRETEERVESLGIERSYQAIVDSDLAL
CCEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHCCCCEE
VVLDLTRPVTAEDASLIARAGARALIVANKCDLPAAGDVPAEAIPVSALTGQGVPELRSA
EEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHCCCHHHCCCCCHHHHHH
ILDAVAPKGVLEHETGFITSLRHEQLLREAVEYLQKAREAVASIPHEMLLLDLYAALRPI
HHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
DAITGATTADDILNRIFSTFCIGK
HHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA