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The map label for this gene is gatA [H]

Identifier: 116620200

GI number: 116620200

Start: 1326021

End: 1327454

Strand: Reverse

Name: gatA [H]

Synonym: Acid_1073

Alternate gene names: 116620200

Gene position: 1327454-1326021 (Counterclockwise)

Preceding gene: 116620201

Following gene: 116620199

Centisome position: 13.32

GC content: 63.25

Gene sequence:

>1434_bases
ATGGATATCCCATCCCTGACCATCGACCGCGTGAGGCAAGGACTGTTCTCGCGCGAGTTCAGCGCCACCGAACTCGCCGC
AGAGGCATTGCGCTTCGCCGAAAACGAGAACCCCAAGACCAACGCGTATCTGCACTTTTCGCCGGAGCGGGCGCTGGCCG
CCGCCGGACGCGTGGACGCCGCGGTGGCGCGCGGCGAAGACCCGGGCGCGCTCGCCGGAGTGCCGGTGGCCGTGAAGGAC
GTGATCGTCACCCAGGGCGTGAAGACCACTTGCGGGTCCCGCCTGCTGGAGCACTACATTCCGCCGTATGACGCCACCGC
CGTCATCCGCATCGAAGAAGCGGGCGGCGTGATCCTGGGCAAGACCAATTGCGATGAGTTCGCCATGGGGTCTTCCAACG
AGAATTCGGCGTTCCGCCCGGTGCGCAACCCGGTCGCGCTCGAACGTGTACCCGGCGGCTCCAGCGGCGGCTCGGCGGCG
GCCGTGGCGCAGGGCACTGCAGTGGTCTCGCTCGGCTCCGACACCGGCGGCTCCATTCGCCAGCCGGCCTCCTTCTGCGG
AATTGTCGGCGTCACGCCGACCTATGGACGCGTCTCGCGCTACGGCCTCACGGCCTTCGCAAGTTCGCTCGATCACATCG
GCCCGTTCGCACGCAACGTGAAGGATGCGGCGACCCTGCTGCGCGTAATGGCCGGCCGTGACCTGCGCGATTCCACCTCC
GCCCCGGCGCCGGTACCGGATTATTCGGCAGACCTGAATAAAAATGTCAAAGGCCTGAAGCTTGGACTCCCCGTCGAGTA
TCTAAGACACTTGACGAGCGAAACCGGTGAACCGCTGAACCGCGGCCTGGAAGTTCTAAAGGCGCTCGGCTGCGAGGTGC
GTGAGATTAGTTTGCCCGCCACCGACTACGCCATTGCGTGCTATTACATCATCGCCACCGCGGAGGCCAGTTCCAACCTC
GCGCGCTATGACGGCGTTCGTTACACCACCCGCTCTGGCGGCGAAACCCTTGCCGATATGTATCGCAACACACGCGGAGA
GGGGTTCGGAGCGGAGTGCAAAAGACGCATCATGTTGGGCACCTATGTGCTCAGTGCGGGATACTATGATGCGTACTATT
TGAAGGCGCAGAAGGTGCGCGCCCTGATCGCGCGCGATTTTGCGAACGCTTTTGGGGAGGTAGATGCCATCGTGGCGCCG
GTGTCGCCTTTCCCGGCCTTTCCGCTGGGAGAAAAAGTCGACGACCCGCTGGCCATGTACCTTTCGGATATTTTTACCAT
CACCGGGAGCCTGGCGGGAATTCCGTGCATGAGCGTACCGTGCGGCAATACTGCCGCGGGCCTGCCGGTGGGGATGCAGA
TTTTGACGAAACATTTCGACGAGTCGACGATGTTCCGCCTCGCCGACGCCTTCGAACACGCACGCAGAGAATAA

Upstream 100 bases:

>100_bases
CATTCCCCCGCTCGGCAATCAGGCCGCGATGGCCAATGCCCCGCAATCGGGCGCGGGCTATTTCAAAGTTCCCAAAGTCA
TCGAACGTTAAAAGACGCGC

Downstream 100 bases:

>100_bases
ACAAGGAGAGATCGCTATGCCATTCACACTTCCCCCGCTGCCCTACGCCCCCGACGCGTTGGAGCCTCACATTGACAAGA
TGACTATGGAGATTCATCAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MDIPSLTIDRVRQGLFSREFSATELAAEALRFAENENPKTNAYLHFSPERALAAAGRVDAAVARGEDPGALAGVPVAVKD
VIVTQGVKTTCGSRLLEHYIPPYDATAVIRIEEAGGVILGKTNCDEFAMGSSNENSAFRPVRNPVALERVPGGSSGGSAA
AVAQGTAVVSLGSDTGGSIRQPASFCGIVGVTPTYGRVSRYGLTAFASSLDHIGPFARNVKDAATLLRVMAGRDLRDSTS
APAPVPDYSADLNKNVKGLKLGLPVEYLRHLTSETGEPLNRGLEVLKALGCEVREISLPATDYAIACYYIIATAEASSNL
ARYDGVRYTTRSGGETLADMYRNTRGEGFGAECKRRIMLGTYVLSAGYYDAYYLKAQKVRALIARDFANAFGEVDAIVAP
VSPFPAFPLGEKVDDPLAMYLSDIFTITGSLAGIPCMSVPCGNTAAGLPVGMQILTKHFDESTMFRLADAFEHARRE

Sequences:

>Translated_477_residues
MDIPSLTIDRVRQGLFSREFSATELAAEALRFAENENPKTNAYLHFSPERALAAAGRVDAAVARGEDPGALAGVPVAVKD
VIVTQGVKTTCGSRLLEHYIPPYDATAVIRIEEAGGVILGKTNCDEFAMGSSNENSAFRPVRNPVALERVPGGSSGGSAA
AVAQGTAVVSLGSDTGGSIRQPASFCGIVGVTPTYGRVSRYGLTAFASSLDHIGPFARNVKDAATLLRVMAGRDLRDSTS
APAPVPDYSADLNKNVKGLKLGLPVEYLRHLTSETGEPLNRGLEVLKALGCEVREISLPATDYAIACYYIIATAEASSNL
ARYDGVRYTTRSGGETLADMYRNTRGEGFGAECKRRIMLGTYVLSAGYYDAYYLKAQKVRALIARDFANAFGEVDAIVAP
VSPFPAFPLGEKVDDPLAMYLSDIFTITGSLAGIPCMSVPCGNTAAGLPVGMQILTKHFDESTMFRLADAFEHARRE
>Mature_477_residues
MDIPSLTIDRVRQGLFSREFSATELAAEALRFAENENPKTNAYLHFSPERALAAAGRVDAAVARGEDPGALAGVPVAVKD
VIVTQGVKTTCGSRLLEHYIPPYDATAVIRIEEAGGVILGKTNCDEFAMGSSNENSAFRPVRNPVALERVPGGSSGGSAA
AVAQGTAVVSLGSDTGGSIRQPASFCGIVGVTPTYGRVSRYGLTAFASSLDHIGPFARNVKDAATLLRVMAGRDLRDSTS
APAPVPDYSADLNKNVKGLKLGLPVEYLRHLTSETGEPLNRGLEVLKALGCEVREISLPATDYAIACYYIIATAEASSNL
ARYDGVRYTTRSGGETLADMYRNTRGEGFGAECKRRIMLGTYVLSAGYYDAYYLKAQKVRALIARDFANAFGEVDAIVAP
VSPFPAFPLGEKVDDPLAMYLSDIFTITGSLAGIPCMSVPCGNTAAGLPVGMQILTKHFDESTMFRLADAFEHARRE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=493, Percent_Identity=37.525354969574, Blast_Score=310, Evalue=3e-84,
Organism=Homo sapiens, GI195972892, Length=483, Percent_Identity=25.6728778467909, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI166795287, Length=238, Percent_Identity=31.5126050420168, Blast_Score=72, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=408, Percent_Identity=41.9117647058824, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17537465, Length=493, Percent_Identity=25.7606490872211, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71990152, Length=326, Percent_Identity=28.8343558282209, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=259, Percent_Identity=32.046332046332, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=197, Percent_Identity=27.9187817258883, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=189, Percent_Identity=32.2751322751323, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=189, Percent_Identity=32.2751322751323, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=427, Percent_Identity=34.192037470726, Blast_Score=220, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6319685, Length=455, Percent_Identity=28.3516483516484, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=225, Percent_Identity=29.7777777777778, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648113, Length=479, Percent_Identity=36.9519832985386, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24648435, Length=501, Percent_Identity=25.1497005988024, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648437, Length=501, Percent_Identity=25.1497005988024, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI45550774, Length=480, Percent_Identity=25, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161078093, Length=499, Percent_Identity=26.0521042084168, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24644968, Length=497, Percent_Identity=23.1388329979879, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652985, Length=485, Percent_Identity=24.5360824742268, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922090, Length=485, Percent_Identity=24.5360824742268, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652981, Length=485, Percent_Identity=24.5360824742268, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652983, Length=485, Percent_Identity=24.5360824742268, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648441, Length=434, Percent_Identity=25.8064516129032, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648439, Length=434, Percent_Identity=25.8064516129032, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21356731, Length=426, Percent_Identity=24.6478873239437, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 50716; Mature: 50716

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00571 AMIDASES ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIPSLTIDRVRQGLFSREFSATELAAEALRFAENENPKTNAYLHFSPERALAAAGRVDA
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHCCHHH
AVARGEDPGALAGVPVAVKDVIVTQGVKTTCGSRLLEHYIPPYDATAVIRIEEAGGVILG
HHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEE
KTNCDEFAMGSSNENSAFRPVRNPVALERVPGGSSGGSAAAVAQGTAVVSLGSDTGGSIR
CCCCCHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEECCEEEEEECCCCCCCCC
QPASFCGIVGVTPTYGRVSRYGLTAFASSLDHIGPFARNVKDAATLLRVMAGRDLRDSTS
CCHHHHCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
APAPVPDYSADLNKNVKGLKLGLPVEYLRHLTSETGEPLNRGLEVLKALGCEVREISLPA
CCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCC
TDYAIACYYIIATAEASSNLARYDGVRYTTRSGGETLADMYRNTRGEGFGAECKRRIMLG
CHHHEEEEEEEEECCCCCCHHHHCCEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
TYVLSAGYYDAYYLKAQKVRALIARDFANAFGEVDAIVAPVSPFPAFPLGEKVDDPLAMY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHH
LSDIFTITGSLAGIPCMSVPCGNTAAGLPVGMQILTKHFDESTMFRLADAFEHARRE
HHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDIPSLTIDRVRQGLFSREFSATELAAEALRFAENENPKTNAYLHFSPERALAAAGRVDA
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHCCHHH
AVARGEDPGALAGVPVAVKDVIVTQGVKTTCGSRLLEHYIPPYDATAVIRIEEAGGVILG
HHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEE
KTNCDEFAMGSSNENSAFRPVRNPVALERVPGGSSGGSAAAVAQGTAVVSLGSDTGGSIR
CCCCCHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEECCEEEEEECCCCCCCCC
QPASFCGIVGVTPTYGRVSRYGLTAFASSLDHIGPFARNVKDAATLLRVMAGRDLRDSTS
CCHHHHCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
APAPVPDYSADLNKNVKGLKLGLPVEYLRHLTSETGEPLNRGLEVLKALGCEVREISLPA
CCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCC
TDYAIACYYIIATAEASSNLARYDGVRYTTRSGGETLADMYRNTRGEGFGAECKRRIMLG
CHHHEEEEEEEEECCCCCCHHHHCCEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
TYVLSAGYYDAYYLKAQKVRALIARDFANAFGEVDAIVAPVSPFPAFPLGEKVDDPLAMY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHH
LSDIFTITGSLAGIPCMSVPCGNTAAGLPVGMQILTKHFDESTMFRLADAFEHARRE
HHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA