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The map label for this gene is ybfF [C]

Identifier: 116620162

GI number: 116620162

Start: 1281126

End: 1281839

Strand: Direct

Name: ybfF [C]

Synonym: Acid_1035

Alternate gene names: 116620162

Gene position: 1281126-1281839 (Clockwise)

Preceding gene: 116620159

Following gene: 116620166

Centisome position: 12.86

GC content: 60.5

Gene sequence:

>714_bases
TTGAACCGATCCATTTCCATTATCATTCCTGCGTACAACGAAGAGAAACGATTGCCCGCGACTCTTATTAAAGTTCGCGA
GTATCTCGACGCTGCAAAGTGGGATTTTGCCGAGATCCTGGTGGTGGACGACGGCTCGCGCGACGGCACCACCAAGGTGG
CATACGGCGCAGGTGTGCGCCTCTTGCGCAATCCCGGCAATCGCGGCAAAGGCTACAGCGTGAAGCACGGCATGCTCGAA
GCCAAAGGCGAATGGTGCTTGTTCACCGACGCCGACCTCTCCTCACCCATCGGCGAATTGGAAAAGTTATGGAACTCCGC
GCAGCGCGAACGCGCCAGCGTGGCCGTCGGTTCCCGCGGCGTCGATCGCTCGCTGGTCGGCGTGCACCAGTCGCCGCTTC
GCGAACTCTCCGGCCGCATTTTTAATCTCGCCATGCGGATCGTGACCGGCCTGCCCTTTAAAGACACGCAGTGCGGTTTC
AAGCTCTTCGAATCCAAAGCCGCCCGCGACGTCTTCTCGCGCCAGCAGCTCGATGGCTTCGGCTTCGACGTCGAGGTGCT
CTTCATCGCCCGCAAGCTGAAGTACAAAGCGATCGAGGTCCCTGTCCGCTGGGACAACGTGGAAGGCACCAAGGTGAGCC
TCTTCCTCGGGGTCACCGCCTTCCTCGACCCCCTCCGCGTCCGCTGGCACGCCTTGACCGGAAAGTACAAGTAG

Upstream 100 bases:

>100_bases
GCTTCGATTTCGCGAATGCGCGTCAAACGCTGTTTGAGCAAGTCGTCTTCCAGGGACAAATAGAACTCCTAACCGGAAAC
AAGCTATTATAAAGCTTCGC

Downstream 100 bases:

>100_bases
AGGAATACTCTAAGCTTTCCTCAGACTTTAATAATCACTTCTCTCAGCCGTTCGCGCGTCAGGAAGAACTTCACGCTCTC
AAACCGTCCGAACAACTTCT

Product: glycosyl transferase family protein

Products: UDP; dolichyl beta-D-glucosyl phosphate

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MNRSISIIIPAYNEEKRLPATLIKVREYLDAAKWDFAEILVVDDGSRDGTTKVAYGAGVRLLRNPGNRGKGYSVKHGMLE
AKGEWCLFTDADLSSPIGELEKLWNSAQRERASVAVGSRGVDRSLVGVHQSPLRELSGRIFNLAMRIVTGLPFKDTQCGF
KLFESKAARDVFSRQQLDGFGFDVEVLFIARKLKYKAIEVPVRWDNVEGTKVSLFLGVTAFLDPLRVRWHALTGKYK

Sequences:

>Translated_237_residues
MNRSISIIIPAYNEEKRLPATLIKVREYLDAAKWDFAEILVVDDGSRDGTTKVAYGAGVRLLRNPGNRGKGYSVKHGMLE
AKGEWCLFTDADLSSPIGELEKLWNSAQRERASVAVGSRGVDRSLVGVHQSPLRELSGRIFNLAMRIVTGLPFKDTQCGF
KLFESKAARDVFSRQQLDGFGFDVEVLFIARKLKYKAIEVPVRWDNVEGTKVSLFLGVTAFLDPLRVRWHALTGKYK
>Mature_237_residues
MNRSISIIIPAYNEEKRLPATLIKVREYLDAAKWDFAEILVVDDGSRDGTTKVAYGAGVRLLRNPGNRGKGYSVKHGMLE
AKGEWCLFTDADLSSPIGELEKLWNSAQRERASVAVGSRGVDRSLVGVHQSPLRELSGRIFNLAMRIVTGLPFKDTQCGF
KLFESKAARDVFSRQQLDGFGFDVEVLFIARKLKYKAIEVPVRWDNVEGTKVSLFLGVTAFLDPLRVRWHALTGKYK

Specific function: Unknown

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI7019323, Length=253, Percent_Identity=35.5731225296443, Blast_Score=131, Evalue=4e-31,
Organism=Homo sapiens, GI215276969, Length=243, Percent_Identity=30.8641975308642, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI4503363, Length=213, Percent_Identity=28.169014084507, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25146207, Length=228, Percent_Identity=34.6491228070175, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71999402, Length=208, Percent_Identity=27.8846153846154, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325029, Length=256, Percent_Identity=31.25, Blast_Score=119, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24582769, Length=228, Percent_Identity=36.8421052631579, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24585265, Length=214, Percent_Identity=28.9719626168224, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: 2.4.1.117

Molecular weight: Translated: 26564; Mature: 26564

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSISIIIPAYNEEKRLPATLIKVREYLDAAKWDFAEILVVDDGSRDGTTKVAYGAGVR
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEECCCEE
LLRNPGNRGKGYSVKHGMLEAKGEWCLFTDADLSSPIGELEKLWNSAQRERASVAVGSRG
EEECCCCCCCCCEECCCCEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
VDRSLVGVHQSPLRELSGRIFNLAMRIVTGLPFKDTQCGFKLFESKAARDVFSRQQLDGF
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GFDVEVLFIARKLKYKAIEVPVRWDNVEGTKVSLFLGVTAFLDPLRVRWHALTGKYK
CCCHHHEEEHHHHCCEEEEEEEEECCCCCCEEEEEEEHHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure
MNRSISIIIPAYNEEKRLPATLIKVREYLDAAKWDFAEILVVDDGSRDGTTKVAYGAGVR
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEECCCEE
LLRNPGNRGKGYSVKHGMLEAKGEWCLFTDADLSSPIGELEKLWNSAQRERASVAVGSRG
EEECCCCCCCCCEECCCCEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
VDRSLVGVHQSPLRELSGRIFNLAMRIVTGLPFKDTQCGFKLFESKAARDVFSRQQLDGF
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GFDVEVLFIARKLKYKAIEVPVRWDNVEGTKVSLFLGVTAFLDPLRVRWHALTGKYK
CCCHHHEEEHHHHCCEEEEEEEEECCCCCCEEEEEEEHHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; dolichyl phosphate

Specific reaction: UDP-glucose + dolichyl phosphate = UDP + dolichyl beta-D-glucosyl phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]